The mass spectrometric data were matched against the in-house data library and the raw data were analyzed usingtidyverse. Metabolites with |log2FoldChange| > 1 and P-value < 0.05 were considered significantly differentially expressed metabolites.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Gas Chromatography MS - positive"],"chromatography_protocol":["The processed samples were analyzed using Agilent 8890/LECO Pegasus BT GC-TOF MS. A 1 μL sample was separated on an Rxi-5ms column (30 m x 0.25 mm x 0.25 μm) with helium flowing at 1 mL/min. The inlet operated in splitless mode at 270 °C. The GC temperature program initiated at 50 °C for 0.5 min, followed by a ramping rate of 5 °C/min until reaching 300 °C over a duration of 6 min. The transfer line and ion source temperatures were 280 and 250 °C, respectively. Mass analysis ranged from 60-700 m/z with an electron energy of 70 eV.
"],"publication":["Metabolomics analysis of antibacterial mechanism."],"submitter_name":["Li Zhang"],"submitter_affiliation":["Sichuan university"],"organism_part":["Whole Organism"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The treated bacterial suspension was centrifuged and washed twice with PBS. Then, 100 μL of pre-chilled methanol: water (8:2) and 10 μL of internal standard were added. The mixture underwent flash-freezing in liquid nitrogen followed by thawing on ice, a process repeated thrice. The sample was then sonicated in an ice bath for 15 min. Following this, 400 μL of pre-chilled methanol:water (8:2) was added, and the mixture was vortexed for 3 min at 4 °C and 1500 rpm. After quenching at -80 °C for 20 min, the sample underwent further sonication for 5 min at 4 °C and centrifugation at 13,000 rpm for 20 min at 4 °C. The resulting supernatant (200 μL) was collected, vacuum-dried and prepared for GC-MS.
"],"organism":["Staphylococcus Aureus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS11717"],"author":["Wei Cheng. chengweilab@163.com.","Zhang Li. Sichuan University. zhangli01@stu.scu.edu.cn."],"data_transformation_protocol":["The mass spectrometric data were matched against the in-house data library and the raw data were analyzed using tidyverse
"],"study_factor":["Timepoint"],"submitter_email":["zhangli01@stu.scu.edu.cn"],"sample_collection_protocol":["Staphylococcus aureus Newman cells cultured overnight were diluted to OD600 = 0.1 using 10 mL TSB medium, and 6-Seleno-GTP with a final concentration of 4 μg/mL was added to the drug treatment groups, and cultured in a flask of 220 rpm at 37 °C until the mid-exponential phase. Among these, 10 duplicate samples were prepared for metabolomics
"],"omics_type":["Metabolomics"],"study_design":["Bacteria","untargeted metabolites","drug"],"curator_keywords":["Bacteria","untargeted metabolites","drug"],"mass_spectrometry_protocol":["The processed samples were analyzed using Agilent 8890/LECO Pegasus BT GC-TOF MS. A 1 μL sample was separated on an Rxi-5ms column (30 m x 0.25 mm x 0.25 μm) with helium flowing at 1 mL/min. The inlet operated in splitless mode at 270 °C. The GC temperature program initiated at 50 °C for 0.5 min, followed by a ramping rate of 5 °C/min until reaching 300 °C over a duration of 6 min. The transfer line and ion source temperatures were 280 and 250 °C, respectively. Mass analysis ranged from 60-700 m/z with an electron energy of 70 eV.
"],"additional_accession":[]},"is_claimable":false,"name":"Metabolomics analysis of antibacterial mechanism","description":"Metabolomics, as a high-throughput analytical technique, enables comprehensive analysis of the metabolic changes in microorganisms under different environmental conditions, revealing the molecular basis of their antibacterial mechanisms. This study employs metabolomics analysis to investigate the antibacterial effects and mechanisms of specific natural products against common pathogenic bacteria.","dates":{"publication":"2025-04-25","submission":"2025-04-25"},"accession":"MTBLS11717","cross_references":{}}