MetaboLights

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2022 was used for peak extraction, alignment and retention time correction of the original data, and the primary and secondary mass errors were controlled within 20 ppm to ensure the accuracy of the identification results. The structure and annotation information of metabolites were obtained by spectrogram comparison of NIST, HMDB, own database and integrated public database.MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phaseWaters ACQUITY UPLC ultra high performance liquid phase systemWaters ACQUITY UPLC BEH C18 Column (1.7 µm, 2.1 mm x 100 mm)Integrated multi-omics analysis and experimental verification reveal the involvement of the PI3K/Akt signaling pathway in myometrial fibrosis of adenomyosis. 10.1038/s41598-025-98369-2. PMID:40254638qiaomei yangDepartment of Gynecology, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, Fujian Province, Chinablankmyometrial tissuemass spectrometry assaySamples were retrieved from -80 °C storage, weighed and ground into powder using liquid nitrogen in a mortar. Each group was then treated with a quadruple volume of MeOH/ACN buffer (1:1, v/v), vortexed and sonicated for lysis. Subsequently, samples were sedimented at -20 °C for 1 h, then centrifuged at 18,000 x g for 15 min at 4 °C to remove cell debris and protein precipitates. The resulting supernatant was transferred to a new tube. After concentration, the insoluble fraction was resuspended with an equal volume of ACN:H2O (1:1, v/v) and centrifuged again.blankHomo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS11965Yang Mei. Fujian Maternity and Child Health Hospital. Department of Gynecology, Fujian Maternity and Child Health Hospital College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, Fujian Province, China.. FJFY2014@163.com. 8.61836E+12.The data acquisition mode uses parallel accumulation serial fragmentation (PASEF) mode. After one level one mass spectrum is acquired, two PASEF mode acquisitions are performed to obtain secondary spectra with parent ion charges within the range of 0-1. The dynamic exclusion time for tandem mass spectrometry scanning is set to 6 s to avoid repeated scans of parent ions.Cohortyangqmf55@yeah.netBriefly, 10 pairs of fresh myometrial tissue samples, with and without adenomyotic lesions, were collected from AM patients who underwent hysterectomiesMetabolomicsFibrosisAdenomyosistargeted metabolitesFibrosisAdenomyosistargeted metabolitesThe data acquisition mode uses parallel accumulation serial fragmentation (PASEF) mode. After one level one mass spectrum is acquired, two PASEF mode acquisitions are performed to obtain secondary spectra with parent ion charges within the range of 0-1. The dynamic exclusion time for tandem mass spectrometry scanning is set to 6 s to avoid repeated scans of parent ions.(+)-IgmesineAdenomyosis (AM) is characterized as a chronic and progressive disorder with limited therapeutic strategies available. Myometrial fibrosis is a prominent pathological feature of AM, yet the underlying molecular mechanisms remain elusive. The present study conducted a comparative analysis using proteomics and metabolomics to investigate myometrial fibrosis and its underlying mechanisms. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) was utilized to analyze adenomyotic and normal myometrial tissues from ten AM patients who underwent hysterectomy with myometrial fibrosis confirmed by Masson staining. This analysis established comprehensive proteomic and metabolomic profiles of AM patients and revealed widespread alterations in the proteome and metabolome within normal and fibrotic myometrium. Key proteins and signaling pathways linked to myometrial fibrogenesis were identified based on proteomic data. The integrated analysis showed significant associations between proteomic and metabolomic data and highlighted the critical role of the PI3K/AKT signaling pathway. Immunohistochemistry and Spearman's correlation analysis suggested a relationship between myometrial fibrosis and the metaplasia of myometrial stromal cells into myofibroblasts. Subsequent experiments identified crucial proteins and signaling pathways involved in myometrial fibrosis, indicating an association with the activation of the PI3K/AKT signaling pathway in myofibroblasts. Notably, PI3K/AKT inhibitors may contribute to the effective alleviation of myometrial fibrosis. This study is the first to demonstrate that myometrial fibrosis represents a critical pathological mechanism in AM through multi-omics methods and to elucidate the crucial role of the PI3K/AKT signaling pathway in this process. These findings provide valuable insights into the pathophysiology of AM and suggest antifibrotic treatment as a promising therapeutic strategy.Integrated multi-omics analysis and experimental verification reveal the involvement of the PI3K/Akt signaling pathway in myometrial fibrosis of adenomyosis.Yang Qiaomei Q, Hong Jingxuan J, Fu Jianhui J, Liu Xianhua X, Zheng Xinye X, Jiang Junying J, Zhu An A, Chen Li L, Lin Hao H, Sun PengMing PfalseIntegrated multi-omics analysis and experimental verification reveal the involvement of the PI3K/Akt signaling pathway in myometrial fibrosis of adenomyosis.This analysis established comprehensive metabolomic profiles of adenomyosis patients and revealed widespread alterations in the metabolome within normal and fibrotic myometrium. Integrated analysis shown significant associations between metabolomic data and fibrosis . This study is the first to demonstrate that myometrial fibrosis represents a critical pathological mechanism in adenomyosis. These findings provide valuable insights into the pathophysiology of zdenomyosis and suggest antifibrotic treatment as a promising therapeutic strategy.2025-05-122025-02-10MTBLS1196540254638