MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/m_MTBLS12373_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/m_MTBLS12373_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/s_MTBLS12373.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/a_MTBLS12373_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/a_MTBLS12373_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_3.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_6.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_2.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_MOCK_5.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/NEG/negative_PRV_1.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_PRV_4.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373/FILES/RAW_FILES/POS/positive_MOCK_3.rawprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12373<p>The data matrix obtained by searching database was uploaded to the Majorbio cloud platform (https://cloud.majorbio.com) for data analysis. Fistly, the data matrix was pre-processed, as follows: At least 80% of the metabolic features detected in any set of samples were retained. After filtering, for specific samples with metabolite levels below the lower limit of quantification, the minimum metabolite value was estimated and each metabolic signature was normalized to the sum. To reduce the errors caused by sample preparation and instrument instability, the response intensities of the sample mass spectrometry peaks were normalized using the sum normalization method, to obtain the normalized data matrix. Meanwhile, the variables of QC samples with relative standard deviation (RSD) &gt; 30% were excluded and log10 logarithmicized, to obtain the final data matrix for subsequent analysis.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>The LC-MS/MS analysis of sample was conducted on a Thermo UHPLC-Q Exactive HF-X system equipped with an ACQUITY HSS T3 column (100 mm x 2.1 mm i.d., 1.8 μm; Waters, USA) at Majorbio Bio-Pharm Technology Co. Ltd (Shanghai, China). The mobile phases consisted of 0.1% formic acid in water:acetonitrile (95:5, v/v) (solvent A) and 0.1% formic acid in acetonitrile:isopropanol:water (47.5:47.5, v/v) (solvent B). The flow rate was 0.40 mL/min and the column temperature was 40 °C.</p>Pseudorabies Virus Triggers Ferritinophagy-Mediated Ferroptosis and Neuroinflammation in Viral Encephalitis.Jiali SunChina Agricultural Universitybrainmass spectrometry assay<p>Briefly, 50 mg solid sample was added to a 2 mL centrifuge tube and a 6 mm diameter grinding bead was added. Next 400 μL of extraction solution (methanol:water, 4:1, v:v) containing 0.02 mg/mL of internal standard (L-2-chlorophenylalanine) was used for metabolite extraction. Samples were ground by the Wonbio-96c (Shanghai wanbo biotechnology co., LTD) frozen tissue grinder for 6 min (-10 °C, 50 Hz), followed by low-temperature ultrasonic extraction for 30 min (5 °C, 40 kHz). The samples were left at -20 °C for 30 min, centrifuged for 15 min (4 °C, 13,000 x g), and the supernatant was transferred to the injection vial for LC-MS/MS analysis.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS12373Yanxin Hu. China Agricultural University. huyx@cau.edu.cn.Jiali Sun. China Agricultural University. 1964606968@qq.com.<p>The pretreatment of LC-MS raw data was performed by Progenesis QI (Waters Corporation, Milford, USA) software, and a 3D data matrix in CSV format was exported. The information in this 3D matrix included: sample information, metabolite name and mass spectral response intensity. Internal standard peaks, as well as any known false positive peaks (including noise, column bleed and derivatized reagent peaks), were removed from the data matrix, deredundant and peak pooled. At the same time, the metabolites were identified by searching database, and the main databases were the HMDB (http://www.hmdb.ca), Metlin (https://metlin.scripps.edu) and Majorbio Database.</p>Group1964606968@qq.com<p>Neuro-2a (N2a) cells were infected with pseudorabies virus (PRV) at a multiplicity of infection (MOI) of 1 for 48 h. Following infection, both PRV-infected and control cells were collected. Specifically, the cells were first digested with trypsin. After trypsin digestion, cells were resuspended in PBS and counted to ensure a density of 10,000,000 cells per sample. The samples were then centrifuged at 1000 x g for 5 min, and the supernatant was discarded. The cell pellets were flash-frozen in liquid nitrogen for 5 min.</p>Metabolomicsuntargeted metabolitesCellvirusPathogenic mechanismuntargeted metabolitesCellvirusPathogenic mechanism<p>The mass spectrometric data were collected using a Thermo UHPLC-Q Exactive HF-X Mass Spectrometer equipped with an electrospray ionization (ESI) source operating in positive mode and negative mode. The optimal conditions were set as followed: source temperature at 425 °C; sheath gas flow rate at 50 arb; Aux gas flow rate at 13 arb; ion-spray voltage floating (ISVF) at -3500 V in negative mode and 3500 V in positive mode, respectively; Normalized collision energy, 20-40-60 V rolling for MS/MS. Full MS resolution was 60000 and MS/MS resolution was 7500. Data acquisition was performed with the Data Dependent Acquisition (DDA) mode. The detection was carried out over a mass range of 70-1050 m/z.</p>Retrofractamide D(+)-Gamma-Hydroxy-L-HomoarginineThreonyllysinePramipexole DihydrochlorideCapric AcidDelta-Hydroxylysylnorleucine8-[(Aminomethyl)Sulfanyl]-6-Sulfanyloctanoic AcidNeoconvallatoxoloside(E)-8-(2-(4-(Diphenylamino)Benzylidene)Hydrazinyl)-N-Hydroxy-8-OxooctanamideXestoaminol CQuingestanolEthyl-1-(2,4,6-Trihydroxy-3-Isobutyrylphenyl)Butyl]-4-Cyclohexene-1,3-DioneProstaglandin D2-1-Glyceryl Ester(2S)-2-Butanol O-[B-D-Apiofuranosyl-(1->6)-B-D-Glucopyranoside]Ophiopogonin CPantothenol1-Nonadecanoyl-Glycero-3-PhosphoserineN-(2,4-Difluorophenyl)-7-Propan-2-Ylpyrazolo[1,5-A]Pyrimidine-5-Carboxamide(3S,4R,5R)-1,3,4,5,6-Pentahydroxy-1-Morpholin-4-Ylhexan-2-One7,8-Diaminopelargonate3-O-Demethylfortimicin A33-Deoxy-33-HydroperoxyfurohyperforinTelocinobufaginAnhydrocinnzeylanine2-Chloro-6-Fluoro-N-(2-Hydroxy-2-Naphthalen-1-Ylethyl)Benzamide4-Amino-N'-Hydroxypyrimidine-5-Carboximidamide[(2-Hexylcyclopentylidene)Amino]ThioureaAnisperimusMonoglyceride CitratePropoxypheneHydromorphonePhenylalanylasparagineNorstictic AcidSer Asn AsnLysylarginineIngenolDifluorodeoxyuridineN2-Acetylornithine1-HexadecanolFructosamine3-Keto Fusidic AcidL-Erythro-Phenylserine20-Carboxy-Leukotriene B4N1,N11-Bis(Ethyl)Norspermine12A-Hydroxy-3-Oxocholadienic AcidHexcer(27:3_2O/18:5)5,8,11-Eicosatrienoic AcidLeustroducsin B4-Propylphenol6-DeoxocastasteroneMethyl PrednisolonateGlutathioneGinkgolide CSer-Phe-LysBisnoryangoninMiglitolEthyl 7-Epi-12-Hydroxyjasmonate GlucosidePseudouridineHexadecylamine3-HexenalCytidine, 2'-Deoxy-2',2'-Difluoro-N-(1-Oxo-2-Propylpentyl)-Vestitone 7-Glucoside4-Aminohex-5-Ynoic AcidPalmitaldehyde27-Deoxy-5B-CyprinolEpimestrol6-Methylthioguanosine MonophosphateLevetiracetam4-Amino-2,2,6,6-Tetramethylpiperidin-1-OlEtodolacB-Raf Inhibitor(3Beta,17Alpha,23S)-17,23-Epoxy-3,28,29-Trihydroxy-27-Norlanost-8-En-24-One5-S-Glutathionyl-Aminochrome ReducedGlycinoeclepin ARosamicinBisindolylmaleimide IvSm(D16:1/2:0)P-BenzosemiquinoneSphingosylphosphorylcholineLactosamine[(2S,3R)-2-Amino-3-Hydroxybutanoyl] (2S)-Pyrrolidine-2-CarboxylateCrocin 5SpisulosineEdoxudine4-Hydroxyphenyllactic AcidMidodrineN-Stearoyl Threonine2-Naphthalen-1-Ylbutanoic AcidN-Docosahexaenoyl LeucineFenvalerateNeamineDulcoside AL-CanalineN'-Nitro-L-ArginineIle Asp ThrCimiaserin B23-AcetoxysoladulcidineDda-Tetracycline7-Aminomethyl-7-CarbaguanineNonylamineCurdioneLysyl-Aspartyl-Glutamyl-LeucineTriterpenoidTyromycic AcidDimethylarsinic Acid(9R,10S)-DihydroxystearateHistidylarginineMuzanzagenin(S)-Hydroxydecanoyl-CoaTyr-GlyH-D-Arg(No2)-OhRitipenemTrypanothione DisulfideAsparaginylarginineCochinchinenin B14-OxolanosterolGamma-GlutamylisoleucineVincosamideLeu Glu ThrVal Gly Asp Ile ValBeraprostPalmitoleamideGlu Arg PheOctane-1,1-DiolPhenylalanyl-Prolyl-Arginine NitrileSucrose MonolaurateHomo-L-Arginine(+/-)-Cp 47,497-D115-Acetamidovalerate2-Propylglutaric AcidPanaxydol Linoleate1-(3-Carboxypropylcarbamoyl)-5-FluorouracilChlorocitric AcidSaxitoxinGingerenone AMatairesinolMidazolamMyotoxin ATyrosyl-ProlineDelta-TocotrienolCay10580N-Propyl-L-ArginineDodecanamide4Alpha-Phorbol 12,13-DidecanoateIsopentyl GentiobiosidePc(Pgj2/P-16:0)GlucoseGlaucarubolone 15-O-Beta-D-Glucopyranoside1-Phenyl-1H-Pyrrole-2,5-Dione3-Hydroxy-Beta-Ionol 3-[Glucosyl-(1->6)-Glucoside]EtimicinDeltorphinMerodesmosineEcgonine(2S)-2-Amino-5-(Diaminomethylideneamino)-4-Methylpentanoic AcidHydroxyethyl Retinamide2-Hydroxyfelbamate7,8-Dihydrovomifoliol 9-[Apiosyl-(1->6)-Glucoside]DibekacinN-(1-Deoxy-1-Fructosyl)GlycineCarbendazim3-Deoxy-D-Glycero-D-Galacto-2-Nonulosonic AcidTyr-Arg-Gln4-Chloro-1H-Indole-3-Acetic Acid1,5-Dihydroxy-8-Methoxycarbonyl-9-Oxoxanthene-3-Carboxylic AcidValeric AcidDecanoyl-Arg-Val-Lys-Arg-Chloromethylketone5-Hydroxylysinonorleucine5-MethylthioriboseBeta-Alanyl-L-LysineBis[2-(2-Butoxyethoxy)Ethyl] HexandioateSialosyl-Tn SaccharideDeoxyhypusineFagomineDeoxycholylasparagineNymphayolCabergolineGlutaminylthreonineN-MonodemethylolopatadineTolmetinSpinosin2-[(E)-(4-Hydroxy-3-Methoxyphenyl)Diazenyl]Benzoic AcidDisoxarilSenegeninBms-275183Obscurolide A2CefradineAlpha-Boswellic AcidDynorphin B (10-13)Ubrogepant3-MethoxyanthranilateCholylthreonineIsopentenyl Adenosine2-Naphthoic AcidArg-Thr-Lys-ArgSabeluzoleN-(1-Deoxy-1-Fructosyl)ValineBeta-D-GlucopyranosylureaBenzyloxycarbonyl-Val-Ala-Asp-FluoromethylketoneBursinAstromicinPalosuranDelta9-Tetrahydrocannabinol Hemisuccinate(+)-ButaclamolAminomalonic Acid4-Vinylcyclohexene3Alpha-Acetomethoxy-11Alpha-Oxo-12-Ursen-24-Oic Acid5-(3-Pyridyl)-2-HydroxytetrahydrofuranSafynolAlpha-Ionol O-[Arabinosyl-(1->6)-Glucoside]Gly Thr LeuDodecylamine16,16-Dimethyl Prostaglandin E2 P-(P-Acetamidobenzamido) Phenyl EsterNocardicin A(24E)-3Alpha-Acetoxy-15Alpha,22S-Dihydroxylanosta-7,9(11),24-Trien-26-Oic Acid3,5-Dimethyl-3'-Isopropyl-L-Thyronine2-Aminoheptanedioic AcidCimifuginCotinine GlucuronideGlycylserylprolylmethionylphenylalanylvalinamide3'-N-Acetyl-4'-O-(14-Methylpentadecanoyl)Fusarochromanone2-UndecanonePsma-Hbed-Cc5-Benzyl-2-[[4-(3-Chlorophenyl)Piperazin-1-Yl]Methyl]-6-Methylpyridazin-3-One7Alpha-HydroxycholesterolIcofungipenMurabutideEnniatin CCucurbic Acid4-Methylcatechol 1-GlucuronideDehydroascorbate (Bicyclic Form)5'-Deoxy-5'-Fluorouridine3-Deoxyarabinohexonic AcidCycloserineGynocardin15-Keto-17-Phenyl Trinor Pgf2AlphaL-Hypoglycin AL-Pyroglutamyl-L-Histidyl-3,3-Dimethylprolinamide5'-GuanidinonaltrindoleHygromycin B4-HydroxymidazolamHexadecasphingosine1,25-Dihydroxy-16-Ene-23-Yne-Vitamin D31,4-IpomeadiolThreonylprolineCytidylyl-(5'-3')-5'-Inosinic Acid HomopolymerBaohuoside ViN-(4-Sulfophenyl)AdenosineAtovaquoneBeta-TyrosineAtenololC(Ccc)C(O)(C[N+](C)(C)C)Cc([O-])=OPe(Pge1/P-18:1(9Z))4-Hydroxyphenylglycine3-Sulfodeoxycholic AcidBisphenol ADyphylline6-ChlorohexanenitrileClethodimHericenone CMelilotoside A(S)-Menthiafolic AcidHericenone DLeu-Leu-Tyr3-[3-[3-Methyl-5-(Oxan-4-Yl)-1,2,4-Triazol-1-Yl]Pyrrolidin-1-Yl]SulfonylpyridineButyl (S)-3-Hydroxybutyrate [Arabinosyl-(1->6)-Glucoside]Muramic Acid13-Demethylspirolide CPi(Pgf1Alpha/20:0)Delphinidin 3-(6'-Acetylgalactoside)Mauritine AN-Docosahexaenoyl Aspartic Acid1-(3-Methyl-2-Butenoyl)-6-ApiosylglucoseSulfamethoxazole N1-GlucuronideCadabicine Methyl Ether2-Aminoethane-1,1,1-TriolIle-Val-IleNemonoxacin1,2-Propanediol, 3-(Octyloxy)-Arg Gln LeuCoformycinAllysine4-HydroxycitrullineSphinganineSecologanateD-Malic Acid P-CoumarateMentholGly Ile Phe3-Hydroxy-4-ButanolideMotolimodN-(2-Ethoxyphenyl)-2-Piperidin-1-Yl-5,6,7,8-Tetrahydroquinazoline-6-Carboxamide(Z)-11-HexadecenalNonanal Propyleneglycol Acetal17-DimethylaminogeldanamycinHeptylamine17-Phenyl-18,19,20-Trinor-Prostaglandin D23-[4-(Sulfooxy)Phenyl]Propanoic AcidGalloyl GlucoseAsi-222VerdamicinMaltolArginine Chloromethyl KetoneDg(6 Keto-Pgf1Alpha/0:0/12:0)EthisteroneCrassostrea SecocarotenoidPolyporenic Acid CEpothilone BEpothilone ADmap-Ethyl-PafDermatan L-IduronateL-Lactic AcidZalcitabineJanthitrem B4-Carbamimidoyl-L-PhenylalanineCarboxymethyl ChitosanAlmorexantFlumethasoneN-Gluconyl EthanolamineBevirimatTsangane L 3-GlucosideTrans-P-Menthane-1,7,8-Triol 8-GlucosidePyroglu-Glu-Asp-Ser-GlyohTenofovir Exalidex1-(Beta-D-Glucopyranosyloxy)-3-OctanoneDg(Pgj2/A-15:0/0:0)DemethylphylloquinolAlbendazole-2-Aminosulfone(14Alpha,17Beta,20S,22R)-14,20-Epoxy-17-Hydroxy-1-Oxowitha-3,5,24-Trienolide4-Aminoisoxazolidin-3-OneJubanine A7-Amino-4-MethylcoumarinD-GlucosaminideOctadecyl FumaratePhe Thr LysCis-Caffeoyl Tartaric AcidN2-Gamma-L-Glutamyl-L-Arginine19Alpha-19-Hydroxy-3,11-Dioxo-12-Ursen-28-Oic AcidAsparagine GlutamateVinblastine2,8-Quinolinediol16Alpha_-Hydroxyprednisolone5-Hydroxycapsanthin-5,6-EpoxideN-[4-[Acetyl(3-Aminopropyl)Amino]Butyl]-N-(3-Aminopropyl)Acetamide2-Hydroxyadipic AcidDeisopropylatrazine4-OxoprolineNb-P-CoumaroyltryptamineCyclo(Aspartylleucylthreonylvalyltyrosylphenylalanylglycyl)N-Acetyl-L-Methionine1,2,10-Trihydroxydihydro-Trans-Linalyl Oxide 7-O-Beta-D-GlucopyranosideColuponePravastatin LactoneTuliposide ADiosbulbin H12-Oxo-20-Dihydroxy-Leukotriene B4SimulenolinePegvaliase-PqpzParomomycin I3H-AdrenalineL-GalactoseSuspensolide FSoyasapogenol EEthyl GlucuronideAsn-Ala3'-Amino-3'-Deoxythimidine Glucuronide2-Propylthiazolo[4,5-C]Quinolin-4-Amine6-Methyl-5,6-Dihydro-4H-1,3-Thiazin-2-AmineErgocornineN-Arachidonoyl Proline3-(Glutathion-S-Yl)Acetaminophen(3R,4R,5R)-3-Fluoro-5-(Hydroxymethyl)Oxolane-2,3,4-TriolImidazolone A5-Phosphonooxy-L-LysinePro Ser IleN-Eicosapentaenoyl TryptophanVitamin D2 3-GlucuronidePtaquilosideWithaferin ASalsolinolChenodeoxycholylcitrullineXylobioseNeohesperidoseSerotoninDiglyceride4-Hydroxy-2-Nonenal-Glutathione ConjugateApo-14'-Zeaxanthinal4(1H)-Pyridinone, 3-Hydroxy-1-(3-Hydroxypropyl)-2-Methyl-L-QuinatePi(Pgf1Alpha/22:3(10Z,13Z,16Z))Prednisone AcetateThienamycinSamidorphanN-Acetyldjenkolic AcidSulfadimidineL-HomocitrullineA-MethapredN-AcetylmannosamineMacrophorin BHydroxymelphalanHexylaminePalmitic AmideAmprenavirNiflumic AcidNylidrin HydrochloridePelargonidin 3-Rhamnoside3-Benzyl-5-(6-Carboxyhexyl)-1-(2-Cyclohexyl-2-Hydroxyethylamino)HydantoinLagerstroemineN-(2-Methoxyphenyl)-6-(3-Phenylpiperazin-1-Yl)Pyridine-2-Carboxamide(S)-5-Amino-3-OxohexanoateCitrusin Ii4Alpha-Carboxy-5Alpha-Cholesta-8,24-Dien-3Beta-OlHypoglycin BPro Gln LeuPentadecylamineKbt 1585 Hydrochloride4-Methylcatechol 2-GlucuronideEnalkirenCortodoxoneChidamideRiginMacrolactin-AFluorohydrocortisone3-Amino-2,3-Dihydrobenzoic AcidEthosuximideGamma-Glutamyl-Beta-CyanoalanineNeobetanin4Alpha-Carboxy-4Beta-Methyl-5Alpha-Cholesta-8,24-Dien-3Beta-OlButyrospermoneLucidenic Acid ASyringetinFumitremorgin BDeoxycholic Acid 3-GlucuronideN-(4-Bromophenyl)-2-(3-Cyclopentyl-2-Oxoimidazo[4,5-B]Pyridin-1-Yl)AcetamideGlutaminylleucine17-AagSyrups, Hydrolyzed Starch, HydrogenatedVaniprevir2-Methyl-N-[(1-Methylbenzimidazol-2-Yl)Methyl]-7-Oxoazepane-2-CarboxamideDexamethasone AcetateN-Lauroyl Arginine3-HydroxysintaxanthinLys Ala Asn Lys(2R,3S)-5-Oxo-3-Phenylmorpholine-2-Carboxylic Acid2-Hydroxypentyl GlucosinolateBetaloneN6-Beta-AspartyllysinePi(6 Keto-Pgf1Alpha/22:5(4Z,7Z,10Z,13Z,16Z))HomocarnosineN-(4-Hydroxyphenyl)-Eicosa-5,8,11,14-TetraenamideKetotrilostane3,3'-Thiobispropanoic Acid16,16-Dimethyl Prostaglandin E11-(11Z-Eicosenoyl)-Glycero-3-Phosphate1-[1-(3-Methoxyphenyl)-2,5-Dioxopyrrolidin-3-Yl]Piperidine-4-Carboxamide16-Phenoxy Tetranor Pgf2AlphaHexyl Heptanoate3-MethoxypropylamineArg-Phe-ArgN-Formylmethionyl-Methionyl-PhenylalanineLysope(14:1(9Z)/0:0)2,2-Bis(1-Hydroxy-2,2,6,6-Tetramethylpiperidin-4-Yl)Decanedioic AcidTryptophyl-PhenylalanineN-Arachidonoyl Alanine3-Oxo-12,18-Ursadien-28-Oic AcidN-Acetyl-L-CarnosineTaraxacolide 1-O-B-D-GlucopyranosideArg-Gln-ArgEthambutolXanthoangelol CFridamycin E(S)-2-Amino-4-Methylsulfanyl-Butane-1-ThiolDalamidAminocaproic AcidGentamicin C2Calcitroic AcidOuabainS-(Formylmethyl)GlutathioneChenodeoxycholylasparagineH-D-Asp(Otbu)-Oh5,6-Dihydroxyprostaglandin F1AVoriconazoleSaccharopine3,N(4)-EthenodeoxycytidinePhylloquinolMicronomicinCucurbitacin ACarglumic AcidMitomycinDeoxycholylglutamic AcidMethionyl-TyrosineCucurbitacin IMedicagenic Acid 3-O-Beta-D-GlucosideApo-12'-ZeaxanthinalCholylhistidineZearalanoneMethyldopaAntibiotic A 204AValidamycin AHalleridoneMotesanib11,17-Dihydroxy-3,20-Dioxopregn-4-En-21-Yl Acetate2,4,4-TrimethylcyclopentanoneIsovalerylglucuronideDeoxycholylprolineAndrosta-1,4,6-Triene-3,17-DioneClavulanateD-DopachromeId14326N-Methoxysuccinyl-Ala-Ala-Pro-ValRipasudilN-Glycolylneuraminic Acid2,3-Dihydroxy-5-Methylthio-4-Pentenoic AcidTrans-Zeatin RibosideAncymidolVal Leu Glu Ser Phe3-Dodecyloxypropane-1,2-DiolCholyllysineCyclocreatine4-Amino-1-[(2R,5R)-5-(Hydroxymethyl)-4-Sulfanyloxolan-2-Yl]Pyrimidin-2-One2-(Dodecyloxy)EthanolResolvin E2LysyltryptophanO2'-4A-Cyclic-TetrahydrobiopterinLucidenic Acid D16-Hydroxy-5-[(4-Sulfophenyl)Azo]-2-Naphthalenesulfonic Acid2-(2-Fluorophenyl)-6-Methylquinoline-4-CarboxamideGlucitol-LysineZizybeoside IiPc(21:0/0:0)(1S,2S,4R)-P-Menth-8-Ene-1,2,10-Triol 2-GlucosideLinalool Oxide D 3-[Apiosyl-(1->6)-Glucoside]DeoxyguanosineIsoleucyl-LysineNylidrinAlpha-Cehc GlucuronideN-Acetyl-Glucosamine 1-PhosphateCaproic AcidAsteltoxin(1R,2S,3R)-2-Acetyl-4(5)-(1,2,3,4-Tetrahydroxybutyl)ImidazoleQuizalofop-EthylHericene BFluorocarazololLeu-Glu1-[(2R,3S,5R)-3,4-Dihydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]Pyrimidine-2,4-DioneDesacetyllevonantradol(-)-AspidospermineLabetalolHexadecenalN-(4-Methylphenyl)Sulonylpiperidine-1-CarboxamideIndoleacetyl Glutamine2-MethylerythritolTebipenem PivoxilUndecanoic Acid1,2-AnhydridoniveusinTiglylglycineFlumazenilPro Ala LeuNona-2,4,6-Trienoyl-CoaP-[(R)-3-[Bis-[(R)-Beta-Hydroxyphenethyl]Amino]Butyl]Benzamide1-(Beta-D-Ribofuranosyl)-1,4-Dihydronicotinamide3'-Hydroxy-4',5',7,8-TetramethoxyflavoneGlobo-HN-Acetyl-Dl-TryptophanAcarviosin3-Oxododecanoic AcidCitroside A3-[3-(Sulfooxy)Phenyl]Propanoic AcidGluten Exorphin B4TaribavirinTetracyclineS-Butylcysteine Sulfoxide1-(2-Hydroxyethyl)-2-Hydroxymethyl-5-NitroimidazoleThr-Ser-Tyr16(17)-Epoxy-21-Acetoxy-5-PregnenoloneTetradecylamineCarbutamideL-Targinine2-Aminobicyclo[2.2.1]Heptane-2-Carboxylic AcidCorynoxeineTrp Trp Leu1-Cyclohexene-1-Carboxylic AcidAngeolidePuerarinGhb-GlucSerylarginineGlutathione Episulfonium IonAk Toxin I2-Aminobicyclo[3.1.0]Hexane-2,6-Dicarboxylic Acid(S)-IsowillardiineGpetn(22:2/18:4)ProparacaineCannabichromevarinMiltironeTernatinTripdiolide15-Octadecene-9,11,13-Triynoic Acid1-Tert-Butyl 4-Ethyl 5-Oxoazepane-1,4-DicarboxylateTetradeca-5,7,9-TrienedioylcarnitineBovileneAcetoxoloneMarinobufagenin5-Amino-6-Ribitylamino UracilMomordenolRutin5'-O-Methylmelledonal4-[2-[[2-(4-Hydroxyphenyl)Acetyl]Amino]Ethylamino]-4-Oxobut-2-Enoic AcidArg Ala Gln LysGlucosyl (2E,6E,10X)-10,11-Dihydroxy-2,6-FarnesadienoateSonchuionoside C3Alpha-(Sulfonatooxy)-5Beta-Cholan-24-Oate1-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-(Hydroxymethyl)Oxolan-2-Yl]Oxypyrimidin-2-One1-Naphthaleneacetic AcidLubiprostone2-Nonene-1,4-DiolEstroneCyanidin 3-(3''-Malonylglucoside)9-(2,3-Dihydroxy-3-Methylbutyl)Furo[3,2-G]Chromen-7-One2-Methyl-4-Oxo-5-Propylthieno[3,2-C]Pyridine-3-Carboxylic Acid5-(Hydroxymethyl-2-Furoyl)GlycinefalsePseudorabies Virus Triggers Ferritinophagy-Mediated Ferroptosis and Neuroinflammation in Viral Encephalitis<p>Pseudorabies virus (PRV), a member of the Alphaherpesvirinae subfamily, primarily infects pigs and poses a significant threat to the swine industry. In recent years, emerging PRV variants have been reported to infect humans, predominantly causing encephalitis. Ferroptosis is a recently identified form of programmed cell death, characterized by iron-dependent lipid peroxidation. It has been implicated in the pathogenesis of various diseases, including neurodegenerative disorders and viral infections. However, whether PRV induces ferroptosis in the central nervous system (CNS) and the underlying mechanisms remain fully elucidated. This study demonstrates that PRV infection induces ferroptosis in N2a cells and murine brains. Specifically, PRV infection triggers ferritinophagy in N2a cells and brain, increasing intracellular free iron levels and subsequent lipid peroxidation, ultimately driving ferroptosis. Furthermore, PRV-induced ferroptosis is closely associated with neuroinflammation. Mechanistically, ferroptosis upregulates prostaglandin-endoperoxide synthase 2 (PTGS2), thereby enhancing prostaglandin E2 (PGE2) synthesis and exacerbating inflammatory responses. Integrated transcriptomic and metabolomic analyses further confirm that PRV-induced ferroptosis drives neuroinflammation through the PTGS2/PGE2 pathway. Notably, treatment with the ferroptosis inhibitor deferoxamine (DFO) effectively mitigates PRV-induced ferroptosis, reduces viral titers in mouse brains, and alleviates viral encephalitis. In conclusion, our study reveals the critical role of ferroptosis in PRV-induced viral encephalitis, providing new therapeutic insights for treating PRV-associated neurological diseases.</p>2026-04-172025-04-02MTBLS12373