After sum-normalization, the processed data were uploaded into before importing into SIMCA-P (version 14.1, Umetrics, Umea, Sweden), where it was subjected to multivariate data analysis, including Pareto-scaled principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). The 7-fold cross-validation and response permutation testing were used to evaluate the robustness of the model. The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Significance was determined using an unpaired Student’s t test. p<0.05 was considered as statistically significant.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic","Liquid Chromatography MS - alternating - reverse phase"],"chromatography_protocol":["Analyses were performed using an UHPLC (1290 Infinity LC, Agilent Technologies) coupled to a QTRAP MS (6500+, Sciex) in Shanghai Applied Protein Technology Co., Ltd. The analytes were separated on HILIC (Waters UPLC BEH Amide column, 2.1 mm × 100 mm, 1.7µm) and C18 columns (Waters UPLC BEH C18-2.1x100 mm, 1.7 μm). For HILIC separation, the column temperature was set at 35 ℃; and the injection volume was 2μL. Mobile phase A: 90% H2O + 2 mM ammonium formate + 10% acetonitrile , mobile phase B: 0.4% formic acid in methanol. A gradient (85% B at 0-1 min, 80% B at 3-4 min, 70% B at 6 min, 50% B at 10-15.5 min, 85% B at 15.6 -23 min ) was then initiated at a flow rate of 300 μL/min. For RPLC separation, the column temperature was set at 40℃, and the injection volume was 2 μL. Mobile phase A: 5 mM ammonium acetate and 0.2% NH3·H2O in water, mobile phase B: 99.5% acetonitrile + 0.5% NH3·H2O.A gradient (5% B at 0 min, 60% B at 5 min, 100% B at 11-13 min, 5% B at 13.1-16 min ) was then initiated at a flow rate of 400 μL/min. The sample was placed at 4 ℃ during the whole analysis process
"],"publication":["WLJP-025p Promote Colonic Epithelial Repair in Ulcerative Colitis through Fatty Acid Oxidation by Regulating Microbial-involvemented SPD Production."],"submitter_name":["Ping Liu"],"submitter_affiliation":["Xinqiao Hospital ARMY Medical University"],"organism_part":["feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["To extract metabolites from the samples, 800 μL of cold Methanol / acetonitrile / water (2:2:1, v/v) extraction solvent was added to 100 mg sample, and adequately vortexed. For absolute quantification of the metabolites, stock solutions of stable-isotope internal standards were added to the extraction solvent simultaneously. Then the samples were under vigorous shaking for 2 min at 4°C and incubated on ice for 20 minutes, and then centrifuged at 14,000 g for 20 minutes at 4°C, the supernatant was collected and flowed through a 96-well protein precipitation plate, and then the elution was collected and dried in a vacuum centrifuge at 4°C. For LC-MS analysis, the samples were re-dissolved in 100 μL acetonitrile/water (1:1, v/v) solvent and and centrifuged at 14000 g at 4 ℃ for 15 min, then the supernatant was injected.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS12400"],"author":["Ping Liu. Xinqiao Hospital ARMY Medical University. Xinqiao Hospital ARMY Medical University. lpiuing@163.com. +8618300916112."],"data_transformation_protocol":["MultiQuant or Analyst was used for quantitative data processing. The QCs were processed together with the biological samples. Metabolites in QCs with coefficient of variation (CV) less than 30 % were denoted as reproducible measurements.
"],"study_factor":["Administration"],"submitter_email":["lpiuing@163.com"],"sample_collection_protocol":["Collect the feces of C57BL/6 mice, immediately immerse them in liquid nitrogen, and store them at -80°C for future use.
"],"omics_type":["Metabolomics"],"study_design":["ultra-performance liquid chromatography-mass spectrometry","ulcerative colitis","Polysaccharide","targeted metabolites"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","ulcerative colitis","Polysaccharide","targeted metabolites"],"mass_spectrometry_protocol":["6500+ QTRAP (AB SCIEX) was performed in positive and negative switch mode. The ESI positive source conditions were as follows: Source temperature: 580℃; Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain Gas (CUR): 35; IonSpray Voltage (IS): +4500 V; The ESI negative source conditions were as follows: Source temperature: 580℃; Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain gas (CUR): 35; IonSpray Voltage (IS) : -4500 V. MRM method was used for mass spectrometry quantitative data acquisition. The MRM ion pairs are showed in the attached file. A polled quality control (QC) samples were set in the sample queue to evaluate the stability and repeatability of the system.
"],"additional_accession":[]},"is_claimable":false,"name":"WLJP-025p Promote Colonic Epithelial Repair in Ulcerative Colitis through Fatty Acid Oxidation by Regulating Microbial-involvemented SPD Production","description":"BACKGROUND & AIMS: Ulcerative colitis (UC) poses a serious threat to human health. WLJP-025p, a polysaccharide component of Lonicera japonica thunb. has been confirmed to have anti-inflammatory and immunomodulatory effects. This study intends to explore the effects and mechanism of WLJP-025p in postponing UC progression by the gut microbiota.
METHODS: The UC model was established by dextran sulfate sodium (DSS) treatment. The fecal microorganisms were detected respectively by adopting the 2bRAD-M technology, and the metabolites were detected by using the H650 medical high-throughput targeted detection. The differentially expressed metabolites (spermidine, SPD) screened out are quantitatively detected by LC-MS. The intestinal microorganisms were cultured in vitro under hypoxic conditions to detect the temporal and spatial changes of WLJP-025p decomposition and spermidine generation. Serum biochemistry and flow cytometry are used to detect inflammatory factors. Immunofluorescence and Western blot are used to assess the intestinal mechanical barrier and the molecular mechanism of WLJP-025p.
RESULTS: WLJP-025p dose-dependently mitigate the symptoms of UC mice, restored the balance of the intestinal microbial population and metabolic disorder in UC. WLJP-025p restored the SPD level and the involvement of microorganisms was sufficient and necessary. SPD activated FAO promoted maturation differentiation of intestinal epithelium, and facilitates barrier repair.
CONCLUSIONS: WLJP-025p restores the SPD balance involved by microorganisms, drives FAO and repairs lipid metabolism disorders and damages. It effectively postpone the progression of UC through the mechanisms of gut microbiota composition and metabolic remodeling.
","dates":{"publication":"2026-05-16","submission":"2025-04-18"},"accession":"MTBLS12400","cross_references":{}}