The R XCMS (v3.12.0) software package was used for peak detection, peak filtering and peak alignment to obtain the list of metabolite quantification, and the spectral databases such as public databases HMDB, massbank, LipidMaps, mzcloud, KEGG and the self-built standard databases were used for the identification of the substances (database searching), and the parameter settings were set to ppm < 30 ppm.,obtaining metabolite characterisation results
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse phase","Liquid Chromatography MS - positive - reverse phase"],"chromatography_protocol":["A Thermo Vanquish (Thermo Fisher Scientific, USA) ultra-high performance liquid chromatography (UPLC) system was used with an ACQUITY UPLC HSS T3 (2.1×100 mm, 1.8 µm) (Waters, Milford, MA, USA) column at a flow rate of 0.3 mL/min and a column temperature of 40 ,and Injection volume 2 μL.
"],"publication":["Toxicological effects of microplastics and antibiotics on enterohepatic axis of Gymnocypris przewalskii."],"submitter_affiliation":["Peking University"],"submitter_name":["Duo Keai"],"organism_part":["intestinal"],"technology_type":["mass spectrometry"],"disease":[""],"extraction_protocol":["Accurately weigh the intestinal contents in a 2 mL centrifuge tube and extract the metabolites by adding 600 µL of methanol (67-56-1, Thermo) containing 2-chloro-L-phenylalanine (103616-89-3, Aladdin).
"],"organism":["Gymnocypris przewalskii"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS12555"],"author":["Ping Zhu. Qing Hai University. pingzhu2024@163.com."],"data_transformation_protocol":["The original mass spectrometer offline file was converted to through the MSConvert tool in the Proteowizard software package (v3.0.8789) mzXML file format. Peak detection, peak filtering and peak alignment processing were carried out using the R XCMS software package , and the parameter Settings were as follows bw=2, ppm=15, peakwidth=c(5, 30), mzwid=0.015, mzdiff=0.01, method= 'centWave', we obtain go to the quantitative list of substances.
"],"study_factor":["Group"],"submitter_email":["keaiduoduo998@126.com"],"sample_collection_protocol":["Fish were obtained from Qinghai Naked Carp Rescue Center, China
All samples were preserved in liquid nitrogen, transported back to the laboratory, and stored long - term at - 80
"],"omics_type":["Metabolomics"],"study_design":["antibiotic","Gymnocypris przewalskii","toxicological effect","microplastics"],"curator_keywords":["antibiotic","Gymnocypris przewalskii","toxicological effect","microplastics"],"mass_spectrometry_protocol":["In positive ion mode, the mobile phases were 0.1% formic acid in acetonitrile (B2) and 0.1% formic acid in water (A2), and the gradient elution program was as follows: 0-1 min, 8% B2; 1-8 min, 8%-98% B2; 8-10 min, 98% B2; 10-10.1 min, 98%-8% B2; 10.1-12 min, 8% B2; negative ion mode, the mobile phases were as follows: 0-1 min, 8% B2; 10-10.1 min, 98%-8% B2; 10.1-12 min, 8% B2. In negative ion mode, the mobile phases were acetonitrile (B3) and 5 mM ammonium formate water (A3), and the gradient elution procedures were: 0~1 min, 8% B3; 1~8 min, 8%~98% B3; 8~10 min, 98% B3; 10~10.1 min, 98%~8% B3; and 10.1~12 min, 8% B3.
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","dates":{"publication":"2025-07-02","submission":"2025-06-05"},"accession":"MTBLS12555","cross_references":{}}