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R XCMS (v3.12.0) software package was used for peak detection, peak filtering and peak alignment to obtain the list of metabolite quantification, and the spectral databases such as public databases HMDB, massbank, LipidMaps, mzcloud, KEGG and the self-built standard databases were used for the identification of the substances (database searching), and the parameter settings were set to ppm &lt; 30 ppm.,obtaining metabolite characterisation results</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>A Thermo Vanquish (Thermo Fisher Scientific, USA) ultra-high performance liquid chromatography (UPLC) system was used with an ACQUITY UPLC HSS T3 (2.1×100 mm, 1.8 µm) (Waters, Milford, MA, USA) column at a flow rate of 0.3 mL/min and a column temperature of 40 ,and Injection volume 2 μL.</p>Toxicological effects of microplastics and antibiotics on enterohepatic axis of Gymnocypris przewalskii.Peking UniversityDuo Keaiintestinalmass spectrometry<p>Accurately weigh the intestinal contents in a 2 mL centrifuge tube and extract the metabolites by adding 600 µL of methanol (67-56-1, Thermo) containing 2-chloro-L-phenylalanine (103616-89-3, Aladdin).</p>Gymnocypris przewalskiihttps://www.ebi.ac.uk/metabolights/MTBLS12555Ping Zhu. Qing Hai University. pingzhu2024@163.com.<p>The original mass spectrometer offline file was converted to through the MSConvert tool in the Proteowizard software package (v3.0.8789) mzXML file format. Peak detection, peak filtering and peak alignment processing were carried out using the R XCMS software package , and the parameter Settings were as follows bw=2, ppm=15, peakwidth=c(5, 30), mzwid=0.015, mzdiff=0.01, method= 'centWave', we obtain&nbsp;go to the quantitative list of substances.</p>Groupkeaiduoduo998@126.com<p>Fish were obtained from Qinghai Naked Carp Rescue Center, China</p><p>All samples were preserved in liquid nitrogen, transported back to the laboratory, and stored long - term at - 80</p>MetabolomicsantibioticGymnocypris przewalskiitoxicological effectmicroplasticsantibioticGymnocypris przewalskiitoxicological effectmicroplastics<p>In positive ion mode, the mobile phases were 0.1% formic acid in acetonitrile (B2) and 0.1% formic acid in water (A2), and the gradient elution program was as follows: 0-1 min, 8% B2; 1-8 min, 8%-98% B2; 8-10 min, 98% B2; 10-10.1 min, 98%-8% B2; 10.1-12 min, 8% B2; negative ion mode, the mobile phases were as follows: 0-1 min, 8% B2; 10-10.1 min, 98%-8% B2; 10.1-12 min, 8% B2. In negative ion mode, the mobile phases were acetonitrile (B3) and 5 mM ammonium formate water (A3), and the gradient elution procedures were: 0~1 min, 8% B3; 1~8 min, 8%~98% B3; 8~10 min, 98% B3; 10~10.1 min, 98%~8% B3; and 10.1~12 min, 8% 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69M226T563M129T403M243T202M152T53_1M171T240M190T291M155T373M188T277M149T252M130T605M227T367M179T81M147T688M245T286M132T214M123T79M212T371M228T53M228T52M128T605M145T604M216T88M167T504M135T46M227T351M135T48M216T83M179T307M242T405M123T70M154T583M167T99M248T53M173T234M197T140M111T43M226T108M111T41M179T69M196T343M188T214M231T486M181T348M219T267M171T616M247T272M232T286M204T290M130T243M230T216M118T483M224T78M188T230M188T221M111T34M111T33M173T239M171T604M219T271M236T50M225T302M224T67M123T45M123T46M155T80M159T429M236T49M172T445M248T73M179T46M223T349M223T347M236T40M212T72M211T140M187T37M225T316M123T41M187T35M173T267M202T223M188T251M111T51M237T523M155T75M179T33M204T42_2M204T42_1M110T343M225T327M187T49M117T238M212T81M225T326M202T70_2M202T70_1M171T664M132T282M167T49M199T34M217T218M223T327M102T659M131T99M155T69M203T430M247T238M244T426M247T232M247T233M167T41M167T43M187T57M154T198M204T48M167T34M191T69_1M191T69_2M110T373M250T526M118T441M199T49M155T53M225T343M155T57M118T67_1M110T364M118T67_2M199T41M118T67_3M237T506M217T201M238T304M187T69M175T267M130T678M197T169M187T66M223T311M204T49M124T582M171T642M131T43_1M131T43_2M143T59M155T42M155T47M155T49M199T53M236T89M152T123M247T252M143T51M248T42M224T94M245T70_2M248T46M248T48M245T70_1M199T69M147T281M174T488M155T34M155T33M166T300M151T312M248T49M145T688falseToxicological effects of microplastics and antibiotics on enterohepatic axis of Gymnocypris przewalskii<p>Metabolites including S-Allylcysteine, trans-Cinnamate,Terephthalate and Misoprostol were significantly altered, along with enrichment in metabolic pathways such as mTOR signaling and oxidative phosphorylation.</p>2025-07-022025-06-05MTBLS12555