MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/m_MTBLS12563_GC-MS___metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/a_MTBLS12563_GC-MS___metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/s_MTBLS12563.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS-GEM-1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS-GEM-2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GEM3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GEM1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/C-2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS-GEM-3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/C-1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GEM2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/C-3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS-GEM-2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS-GEM-1.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS2.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/BS-GEM-3.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563/FILES/DERIVED_FILES/GS1.mzMLprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12563The standard curve equation is obtained according to the mass spectrum signal information of standard samples with different concentrations, and then each sample is automatically calculated by using the standard curve. The concentration information of different metabolites in the sample is used to calculate the content information of the final sample according to the initial mass or volume of the sample.MetaboLightsPublicGas Chromatography MS -Samples were separated on Agilent DB-FFAP capillary column (30 m x 250 μm x 0.25 μm) gas chromatography system. Programmed heating: initial temperature 90 °C; 10 °C/min to 160 °C; 40 °C/MIN to 240 °C and maintained for 5 min. The carrier gas was helium with a flow rate of 1.0 mL/min.Long-lasting effects of oral polysaccharides on chemotherapy efficacy via alteration of the gut microbiota in a mouse model.Zhejiang Guangsha Vocational and Technical University of ConstructionDing boshengSerummass spectrometryThaw the sample on ice and place the sample in a 2 mL centrifugal tube. 50 ΜL of 20% phosphoric acid was resuspended, and 4-methylvaleric acid with a final concentration of 500 μm was added as an internal standard, which was shaken and mixed for 2 min. The supernatant was centrifuged at 14000 g for 20 min, split Ratio 10:1, split injection.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS12563Zhangjun Song. Translational medicine Center of Shaanxi Provincial People's Hospital. songzhj0410@163.com.Zhang jun Song. Translational medicine Center of Shaanxi Provincial People's Hospital. songzhj0410@163.com.MSD ChemStation software was used to extract chromatographic peak area and retention time. Draw a calibration curve and calculate the amount of short-chain fatty acid in the sample.Gemcitabine; polysaccharide15700153239@163.comBlood samples were collected (500 μl-1 mL) from the mouse orbit from the mouse postmortem and centrifuged for 3000 rpm for 15 minutes, and supernatants needed to be collected.Metabolomicspolysaccharidebreast cancergemcitabineimmunoenhancing factorpolysaccharidebreast cancergemcitabineimmunoenhancing factorAgilent 5977B MSD mass spectrometer was used for mass spectrometric analysis. The conditions of 5977B MSD were as follows: injection port temperature 250 °C, ION source temperature 230 °C, transmission line temperature 250 °C, quadrupole temperature 150 °C. Electron impact ionization (EI) source, electron energy 70 ev; SCAN/SIM mode was used to detect the analyte.Acetic acidfalseLong-lasting effects of oral polysaccharides on chemotherapy efficacy via alteration of the gut microbiota in a mouse modelGemcitabine always has a short-term immune effect, and we consider using oral polysaccharides to lengthen its immune effect by modulating gut microbiotas. To verify this, we built a breast cancer mice model in situ, oral polysaccharides and a low-dose of Gemcitabine were administrated, and we found combined treatment has a certain immune enhancement effect through immunological-related experiments and tumor measurement. Additionally, 16s rRNA sequencing and metabonomics studies found combination treatment increases Butyric Acid and Trptophan and reshapes the gut microbiota.2025-06-142025-06-06MTBLS12563