Metabolites were identified and confirmed using PeakView software (SCIEX) based on accurate mass and MS/MS spectra.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive - reverse phase"],"chromatography_protocol":["Separation was performed on a reverse-phase ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with water (0.1% formic acid) as mobile phase A and acetonitrile/isopropanol (70:30, 0.1% formic acid) as mobile phase B, at 0.3 mL/min and 40 °C.
"],"publication":["12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII."],"submitter_affiliation":["The OSU"],"submitter_name":["Shinsuke Nirengi"],"organism_part":["blood plasma"],"technology_type":["mass spectrometry"],"disease":[""],"extraction_protocol":["Serum (100 μL) was extracted using methanol and methyl tert-butyl ether (MTBE), followed by water-induced phase separation. The organic phase was collected, purified with C18 SPE cartridges, dried under nitrogen, and reconstituted in 50% methanol.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS12646"],"author":["Stanford kristin. The Ohio State University. 314 Hamilton Hall 460 W. 12th Ave.. Kristin.Stanford@osumc.edu. +1 (614) 247-8287.","Shinsuke Nirengi. The Ohio State University. 314 Hamilton Hall 460 W. 12th Ave.. shinsuke.nirengi@osumc.edu. +1 (614) 247-8287."],"data_transformation_protocol":["Quantification was performed using MultiQuant software (SCIEX). Calibration curves were constructed from 15 standard concentrations (0.01–1000 pg/μL). Internal standards were used for normalization.
"],"study_factor":["Age"],"submitter_email":["Shinsuke.Nirengi@osumc.edu"],"sample_collection_protocol":["Liquid chromatography mass spectrometry (LC-MS/MS) on plasma of young (aged 24-40) and aged (aged 65-90) human subjects.
"],"omics_type":["Metabolomics"],"study_design":["Heart","Brown Adipose Tissue","aging","12,13-DiHOME"],"curator_keywords":["Heart","Brown Adipose Tissue","aging","12,13-DiHOME"],"mass_spectrometry_protocol":["MS analysis was performed using SCIEX TripleTOF 6600+ in high-resolution multiple-reaction monitoring mode. Electrospray ionization (positive mode) was used with a TOF-MS/MS method loop.
"],"metabolite_name":["Arachidonic acid"],"additional_accession":[]},"is_claimable":false,"name":"12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII.","description":"Liquid chromatography mass spectrometry (LC-MS/MS) on plasma of young (aged 24-40) and aged (aged 65-90) human subjects.
","dates":{"publication":"2025-06-26","submission":"2025-06-26"},"accession":"MTBLS12646","cross_references":{}}