MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/m_MTBLS12646_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/s_MTBLS12646.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/a_MTBLS12646_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/DERIVED_FILES/Lipidomics_Plasma_AgeYoung.mzMLftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged11.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged7.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged12.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged10.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged9.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged8.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Young5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646/FILES/RAW_FILES/Aged6.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12646<p>Metabolites were identified and confirmed using PeakView software (SCIEX) based on accurate mass and MS/MS spectra.</p>MetaboLightsPublicLiquid Chromatography MS - positive - reverse phase<p>Separation was performed on a reverse-phase ACQUITY UPLC BEH C18 column (2.1 × 100 mm, 1.7 μm) with water (0.1% formic acid) as mobile phase A and acetonitrile/isopropanol (70:30, 0.1% formic acid) as mobile phase B, at 0.3 mL/min and 40 °C.</p>12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII.The OSUShinsuke Nirengiblood plasmamass spectrometry<p>Serum (100 μL) was extracted using methanol and methyl tert-butyl ether (MTBE), followed by water-induced phase separation. The organic phase was collected, purified with C18 SPE cartridges, dried under nitrogen, and reconstituted in 50% methanol.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS12646Stanford kristin. The Ohio State University. 314 Hamilton Hall 460 W. 12th Ave.. Kristin.Stanford@osumc.edu. +1 (614) 247-8287.Shinsuke Nirengi. The Ohio State University. 314 Hamilton Hall 460 W. 12th Ave.. shinsuke.nirengi@osumc.edu. +1 (614) 247-8287.<p>Quantification was performed using MultiQuant software (SCIEX). Calibration curves were constructed from 15 standard concentrations (0.01–1000 pg/μL). Internal standards were used for normalization.</p>AgeShinsuke.Nirengi@osumc.edu<p>Liquid chromatography mass spectrometry (LC-MS/MS) on plasma of young (aged 24-40) and aged (aged 65-90) human subjects.&nbsp;</p>MetabolomicsHeartBrown Adipose Tissueaging12,13-DiHOMEHeartBrown Adipose Tissueaging12,13-DiHOME<p>MS analysis was performed using SCIEX TripleTOF 6600+ in high-resolution multiple-reaction monitoring mode. Electrospray ionization (positive mode) was used with a TOF-MS/MS method loop.</p>Arachidonic acidfalse12,13-diHOME protects against the age-related decline in cardiovascular function via attenuation of CaMKII.<p>Liquid chromatography mass spectrometry (LC-MS/MS) on plasma of young (aged 24-40) and aged (aged 65-90) human subjects.&nbsp;</p>2025-06-262025-06-26MTBLS12646