The identification of metabolites was based on self-built library PSNGM Database, mzCloud online library (https://www.mzcloud.org), LIPID MAPS (https://www.lipidmaps.org), HMDB (https://hmdb.ca), MoNA (https://mona.fiehnlab.ucdavis.edu) and NIST_2020_MSMS spectral library. The MS1 mass tolerance was set to 15 ppm and the MS2 Match Factor Threshold was set to 50.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive","Liquid Chromatography MS - negative"],"chromatography_protocol":["An Thermo Scientific Vanquish Flex UHPLC System and ACQUITY UPLC HSS T3 column (100Å, 1.8 µm, 2.1 mm x 100 mm; Waters) were used, with a flow rate of 0.4 mL/min, a column temperature of 40 °C, an autosampler temperature of 8 °C and an injection volume of 2 μL. Positive and negative mode mobile phase: mobile phase A is 0.1% formic acid water, mobile phase B is acetonitrile (containing 0.1% formic acid), and the elution gradient was 0 min, 95% A; 1 min, 95% A; 7 min, 5% A; 7-8 min, 5% A; 8.1 min, 95% A; 8.1-12 min, 95% A.
"],"publication":["Maternal chromium propionate reprograms the gut-blood-placenta axis to support neonatal immune and metabolic development in dairy calves: a multi-omics insight."],"submitter_name":["yuzhen Sui"],"submitter_affiliation":["North West Agriculture and Forestry University"],"organism_part":["faeces"],"technology_type":["mass spectrometry"],"disease":[""],"extraction_protocol":["50 mg of sample were transferred to a 2 mL centrifuge tube and 200 μL of pre-cooled methanol:acetonitrile (1:1, v/v) were added. Samples were vortexed for 30 s and then kept at -20 °C for 30 min. After this step, samples were centrifuged at 12,000 rpm and 4 °C for 10 min and 200 μL of the supernatant were collected and concentrated to dryness under vacuum. 100 μL 50% methanol (containing 5 ppm 2-chlorophenylalanine) were added and samples were vortexed for 30 s and then samples were centrifuged at 12,000 rpm and 4 °C for 10 min, filtered the supernatant through a 0.22 μm filter membrane. 10-20 μL of each sample filtrate were taken and mixed into a QC sample for evaluating instrument stability and data reliability.
"],"organism":["dairy cow"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS12695"],"author":["Sui yuzhen. North West Agriculture and Forestry University. sui1632020@163.com."],"data_transformation_protocol":["The raw format data was imported into the commercial software Compound Discoverer 3.3 (version 3.3.2.31, Thermo, Waltham, USA). Based on the new peak detection and peak quality scoring algorithm of the software, peak extraction, alignment, correction and other operations were performed. The unique peak quality rating calculation and filter greatly reduced the interference of background peaks and low-quality peaks. The peaks that were not detected in more than 50% of the QC samples were filtered, and the missing alues of the undetected peaks were filled based on the software Fill Gaps algorithm, and the Sum total peak area was normalized.
"],"study_factor":["Treatment"],"submitter_email":["sui1632020@163.com"],"sample_collection_protocol":["This study was conducted from January to February 2024 at a large commercial dairy farm operated by Hebei Shounong Modern Agricultural Science and Technology Co. located in Hebei Province, China (114.99°E, 38.51°N). Thirty Holstein cows with similar age, litter size, body condition score (BCS) and expected date of delivery were randomly selected 21 days before the expected date of delivery and transferred to a pre-partum feeding facility. Upon admission, the cows were randomized into three groups of 10 cows each: a control group (Con), a low-dose CrPro group (CrL) and a high-dose CrPro group (CrH). The three groups were supplemented with 0, 1 and 2 g CrPro/(head-day), corresponding to (trivalent chromium) doses of 0, 4 and 8 mg/(head-day), respectively. CrPro was supplemented twice a day, at 08:00 am and 17:00 pm. On the day of calving, fresh fecal samples were collected from the rectum of heifers, placed in sterile 50 mL centrifuge tubes and stored at -80°C until further analysis.
"],"omics_type":["Metabolomics"],"study_design":["Calf","untargeted metabolites","faeces specimen"],"curator_keywords":["Calf","untargeted metabolites","faeces specimen"],"mass_spectrometry_protocol":["Thermo Orbitrap Exploris 120 mass spectrometer was used to collect DDA mass spectrometric data in positive and negative ion modes under the control of Xcalibur software (version: 4.7, Thermo). HESI source, spray voltage 3.5 kV / -3.0 kV, sheath gas 40 arb, auxiliary gas 15 arb, capillary temperature 325 °C, auxiliary gas temperature 300 °C, primary resolution 60,000, scan range 100-1000 m/z, AGC Target Standard, Max IT 100 ms, the top 4 ions were screened for secondary fragmentation, dynamic exclusion time was 8 s, secondary resolution 15,000, HCD collision energy 30%, AGC Target Standard, Max IT Auto. All formal samples and QC sample were loaded into the equipment according to the above-mentioned chromatography and mass spectrometry methods. Before the formal injection, QC sample were injected 2-4 times to balance the system. During the injection process, one QC sample was injected for every 5-10 samples for subsequent data evaluation and quality control.
"],"additional_accession":[]},"is_claimable":false,"name":"Maternal chromium propionate reprograms the gut-blood-placenta axis to support neonatal immune and metabolic development in dairy calves: a multi-omics insight","description":"In this study, non-targeted metabolomics sequencing was performed on fecal samples from dairy cows to investigate the effect of chromium propionate supplementation on fecal metabolism in periparturient dams
","dates":{"publication":"2025-07-09","submission":"2025-07-08"},"accession":"MTBLS12695","cross_references":{}}