MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/m_MTBLS12696_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/m_MTBLS12696_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/a_MTBLS12696_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/s_MTBLS12696.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/a_MTBLS12696_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH7-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL1-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC1-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con5-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH6-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con7-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL2-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC7-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con1-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL3-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con4-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con3-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con2-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC6-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC5-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL6-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL7-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC3-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con7-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC4-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL5-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL4-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH4-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC1-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC2-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH1-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH3-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH2-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con4-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con3-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL2-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH5-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con6-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH6-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH7-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL4-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con5-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL1-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con1-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/Con2-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL5-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrH1-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC7-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/CrL6-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC6-pos.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/FILES/QC5-neg.rawftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12696/F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identification of metabolites was based on self-built library PSNGM Database, mzCloud online library (https://www.mzcloud.org), LIPID MAPS (https://www.lipidmaps.org), HMDB (https://hmdb.ca), MoNA (https://mona.fiehnlab.ucdavis.edu) and NIST_2020_MSMS spectral library. The MS1 mass tolerance was set to 15 ppm and the MS2 Match Factor Threshold was set to 50.MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phaseAn Thermo Scientific Vanquish Flex UHPLC System and ACQUITY UPLC HSS T3 column (100Å, 1.8 µm, 2.1 mm x 100 mm; Waters) were used, with a flow rate of 0.4 mL/min, a column temperature of 40 °C, an autosampler temperature of 8 °C and an injection volume of 2 μL. Positive and negative mode mobile phase: mobile phase A is 0.1% formic acid water, mobile phase B is acetonitrile (containing 0.1% formic acid), and the elution gradient was 0 min, 95% A; 1 min, 95% A; 7 min, 5% A; 7-8 min, 5% A; 8.1 min, 95% A; 8.1-12 min, 95% A.Maternal chromium propionate reprograms the gut-blood-placenta axis to support neonatal immune and metabolic development in dairy calves: a multi-omics insight.yuzhen SuiNorth West Agriculture and Forestry Universityplasmamass spectrometry50 μL of sample were transferred to a 2 mL centrifuge tube and 200 μL of pre-cooled methanol:acetonitrile (1:1, v/v) were added. Samples were vortexed for 30 s and then kept at -20 °C for 30 min. After this step, samples were centrifuged at 12,000 rpm and 4 °C for 10 min and 200 μL of the supernatant were collected and concentrated to dryness under vacuum. 100 μL 50% methanol (containing 5 ppm 2-chlorophenylalanine) were added and samples were vortexed for 30 s and then samples were centrifuged at 12,000 rpm and 4 °C for 10 min, filtered the supernatant through a 0.22 μm filter membrane. 10-20 μL of each sample filtrate were taken and mixed into a QC sample for evaluating instrument stability and data reliability. dairy cowhttps://www.ebi.ac.uk/metabolights/MTBLS12696Sui Yuzhen. North West Agriculture and Forestry University. sui1632020@163.com.The raw format data was imported into the commercial software Compound Discoverer 3.3 (version 3.3.2.31, Thermo, Waltham, USA). Based on the new peak detection and peak quality scoring algorithm of the software, peak extraction, alignment, correction and other operations were performed. The unique peak quality rating calculation and filter greatly reduced the interference of background peaks and low-quality peaks. The peaks that were not detected in more than 50% of the QC samples were filtered, and the missing alues of the undetected peaks were filled based on the software Fill Gaps algorithm, and the Sum total peak area was normalized.Treatmentsui1632020@163.comThis study was conducted from January to February 2024 at a large-scale commercial dairy farm operated by Hebei Shounong Modern Agricultural Technology Co., Ltd., located in Hebei Province, China (114.99°E, 38.51°N). Twenty-one days prior to the expected calving date, thirty Holstein dairy cows with similar age, parity, body condition score (BCS), and expected parturition date were randomly selected and transferred to the prepartum housing facility. Upon admission, the cows were randomized into three groups: control (Con), low-dose CrPro group (CrL) and high-dose CrPro group (CrH), each with 10 cows. The three groups were supplemented with 0, 1, and 2 g/(head-day) of CrPro, which corresponded to (Cr (III)) doses of 0, 4, and 8 mg/(head-day), respectively. The CrPro supplementation was administered twice a day, at 08:00 am and 17:00 in two feedings. On the day of calving, tail vein blood was collected from the dam. All blood samples were drawn into 10-mL lithium heparin-coated vacuum tubes. Plasma was obtained by centrifugation at 3000 rpm for 10 min and subsequently stored at -80°C for further analysis.MetabolomicsPlasmaCalfuntargeted metabolitesPlasmaCalfuntargeted metabolitesThermo Orbitrap Exploris 120 mass spectrometer was used to collect DDA mass spectrometric data in positive and negative ion modes under the control of Xcalibur software (version: 4.7, Thermo). HESI source, spray voltage 3.5 kV / -3.0 kV, sheath gas 40 arb, auxiliary gas 15 arb, capillary temperature 325 °C, auxiliary gas temperature 300 °C, primary resolution 60,000, scan range 100-1000 m/z, AGC Target Standard, Max IT 100 ms, the top 4 ions were screened for secondary fragmentation, dynamic exclusion time was 8 s, secondary resolution 15,000, HCD collision energy 30%, AGC Target Standard, Max IT Auto. All formal samples and QC sample were loaded into the equipment according to the above-mentioned chromatography and mass spectrometry methods. Before the formal injection, QC sample were injected 2-4 times to balance the system. During the injection process, one QC sample was injected for every 5-10 samples for subsequent data evaluation and quality control.falseMaternal chromium propionate reprograms the gut-blood-placenta axis to support neonatal immune and metabolic development in dairy calves: a multi-omics insightThis study sequenced the non-target metabolome of maternal plasma samples from dairy cows to investigate the effect of chromium propionate supplementation on maternal plasma metabolism in the pre-peripartum period.2025-07-092025-07-08MTBLS12696