MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/m_MTBLS12711_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/m_MTBLS12711_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/s_MTBLS12711.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/a_MTBLS12711_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711/a_MTBLS12711_LC-MS_negative_reverse-phase_metabolite_profiling.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12711<p>The components eluted after chromatographic separation continuously enter the mass spectrometer, and the mass spectrometer continuously scans for data acquisition. One mass spectrum is obtained for each scan. By continuously plotting the ions with the greatest intensity in each mass spectrum, with the ion intensity as the ordinate and time as the abscissa, the resulting chromatogram is the Base Peak Chromatogram (BPC). </p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>The LC analysis was performed using an Ultimate 3000 UHPLC System (Thermo Fisher Scientific, USA). Chromatographic separation was carried out on an ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm) (Waters, Milford, MA, USA), maintained at a temperature of 40 °C. The flow rate and injection volume were set at 0.3 mL/min and 2 μL, respectively. </p><p><br></p><p><br></p>Metabolomic analysis of seminal fluids in oligoasthenospermia individuals.Zhejiang UniversityKezhen Yaoseminal plasmamass spectrometry<p>1. Thaw the experimental sample at 4℃, vortex the sample for 1 min after thawing, and mix</p><p>evenly;</p><p>2. Accurately transfer an appropriate amount of sample into a 2 mL centrifuge tube;</p><p>3. Add 400 µL methanol and vortex for 1 min;</p><p>4. Centrifuge for 10 min at 12,000 rpm and 4 °C,take all the supernatant transfer it to a new 2 mL</p><p>centrifuge tube, concentrate and dry it;</p><p>5. Add 150 µL of 2-chloro-l-phenylalanine (4 ppm) solution prepared with 80% methanol water</p><p>to redissolve the sample, filter the supernatant by 0.22 μm membrane and transfer into the</p><p>detection bottle for LC-MS detection.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS12711Yao Kezhen. ZHEJIANG UNIVERSITY. kezhenyao@zju.edu.cn.<p>The raw mass spectrometry output files were converted into the mzXML file format using the MSConvert tool in the Proteowizard software package (v3.0.8789) . The R XCMS software package was employed for peak detection, peak filtering, and peak alignment . The parameter settings were bw = 2, ppm = 15, peakwidth = c(5, 30), mzwid = 0.015, mzdiff = 0.01, and method = 'centWave', resulting in a list of substance quantifications. Support vector regression correction based on quality control (QC) samples was used to eliminate systematic errors. Then, substances with a coefficient of variance (CV) less than 30% in the QC samples were retained for subsequent analysis. </p>Diseasekezhenyao@zju.edu.cn<p>Semen samples were collected via masturbation, which is the standard methodology for semen acquisition. All donors maintained abstinence for 3–7 days prior to providing specimens and washed their hands with soap and water before self-collection into sterile, wide-mouthed sample cups in a room adjacent to the andrology laboratory. Donors were instructed to collect only the initial ejaculate while ensuring no spilled semen was included. Caps were immediately applied after collection, and the specimens were promptly placed in a 37 °C water bath until complete liquefaction occurred prior to analysis.</p>MetabolomicsOligoasthenospermiauntargeted metabolitesAssisted Reproductive TechnologyOligoasthenospermiauntargeted metabolitesAssisted Reproductive Technology<p>Mass spectrometric detection of metabolites was performed on Q Exactive(Thermo Fisher</p><p>Scientific, USA) with ESI ion source. Simultaneous MS1 and MS/MS (Full MS-ddMS2 mode,</p><p>data-dependent MS/MS) acquisition was used. The parameters were as follows: sheath gas</p><p>pressure, 40 arb; aux gas flow, 10 arb; spray voltage, 3.50 kV and -2.50 kV for ESI(+) and ESI(-),</p><p>respectively; capillary temperature, 325 ℃ ; MS1 range, m/z 100-1000; MS1 resolving power,</p><p>70000 FWHM; number of data dependant scans per cycle, 10; MS/MS resolving power, 17500</p><p>FWHM; normalized collision energy, 30 eV; dynamic exclusion time, automatic</p>PlantagoninePhenylpyruvateCarboprost TromethamineWuweizisu ALysoPE(18_1(11Z)_0_0)(2Z,4E,6E,8E)-3,7-Dimethyl-9-(2,6,6-trimethylcyclohex-1-en-1-yl)nona-2,4,6,8-tetraenalProstaglandin G2(E)-3-(2-Hydroxyphenyl)-2-propenalN-Fructosyl isoleucineN1,N10-DicoumaroylspermidineACMC-20my24GuaiacolDeoxyribose 1-phosphateLunarinegamma-Glutamyl-beta-cyanoalanineFA 20_5;O4CitrullineFA 20_5;O23beta-Acetoxy-19alpha-hydroxy-12-ursene15-HPETEMethyl indole-3-acetate6-hydroxynicotinecis-p-Coumaric acidIndigo4-HydroxybenzaldehydeHexanoylcarnitine5-Hydroxy-7-docosanoneLysoPC(15_0_0_0)9-cis-Retinoic acid14,15-EpETERoquefortine FPinostilbene4,4-Diaponeurosporen-4-alEstradiolcis-2,3-Dihydro-2,3-dihydroxybiphenylChloroxineAbscisic alcohol4-Methylcatechol7-DehydrodesmosterolGlutamylisoleucine3,4-DihydroxymandelaldehydeLysoPE (16_0_0_0)(-)-ThebaineFluocinonide1,2-Bis(4-hydroxyphenyl)-2-propanolbeta-JononeBrassilexinOleamideEthionamide sulphoxide3-phosphoglycerateDihydrochelerythrineBicalutamideLysoPC(22_4(7Z,10Z,13Z,16Z)_0_0)(6Z,10E,14E)-3,7,11,15,19-Pentamethylicosane-6,10,14,18-tetraene-1-olGlyceollin I6-Benzylaminopurinedihydromonacolin L acid3,4-Dihydro-3-hydroxy-4-S-glutathionyl bromobenzeneProstaglandin C1Autumnaline3a,21-Dihydroxy-5b-pregnane-11,20-dioneL-OrnithineQuinethazonePhthalic acidLysoPC(18_1(11Z)_0_0)5-MethoxytryptamineMethyl indole-3-propanoateProstaglandin D32,4-QuinolinediolArginylalanine2-Pentanone2-Hexaprenyl-3-methyl-6-methoxy-1,4 benzoquinoneMG(0_0_16_0_0_0)1,1,1-TrichloroethaneLycopersiconolide3a,7b,21-Trihydroxy-5b-cholanoic acidIndoleProstaglandin A1beta-Ionone13,14-Dihydro-15-keto-PGD2Isocitric acidLysoPC(20_3(8Z,11Z,14Z)_0_0)Phenylalanylglycine1H-Pyrrolo[2,1-c][1,4]thiazine3,5-Dibromo-4-hydroxybenzoatePE(18_0_20_4(5Z,8Z,11Z,14Z))Dihydroxyfumitremorgin Calpha-Tocopherol acetate(S)-2-Aceto-2-hydroxybutanoic acid3-hydroxyechinenone1,4-Dihydronicotinamide adenine dinucleotideBornyl_acetateLinoleic acidN-Acetyl-D-hexosamineMethyleugenolCarnosic_acidBenzoic acidL-PhenylalanineN-alpha-acetylornithineRebeccamycin4-Methylumbelliferyl acetateN-n-Butyl-N-methyl-11-(3,17beta-dihydroxyestra-1,3,5(10)-trien-7alpha-yl)undecanamide1-Hydroxymethylnaphthalene4-(Glutamylamino) butanoatePropanoyl phosphate6-hydroxy-N-methylmyosmine2,4-diacetamido-2,4,6-trideoxy-alpha-D-mannopyranose3-DehydrosphinganineSphingosineBiphenylL-histidinol-phosphateEthyl 7-epi-12-hydroxyjasmonate glucosideAlanylglutamic acidnephthenolPiceolGibberellin A85-Hydroxy-7-tetracosanoneN,N,N-Trimethyl-L-alanyl-L-proline betaineTelmisartanSM(d16_1_16_0)Tyrosine methylester(E)-26,27-Dinorergosta-4,22-dien-3-one3-geranyl-3-[(Z)-2-isocyanoethenyl]-1H-indole(1S)-Hydroxy-(2S)-glutathionyl-1,2-dihydronaphthaleneST 21_3;O5BimatoprostPhenylacetaldehydeD-ArabinoseGeranyl acetate2-(3-Hydroxy-4-methylphenyl)-5-methyl-4-hexen-3-oneLysoPE(P-18_0_0_0)PC(20_5(5Z,8Z,11Z,14Z,17Z)_P-16_0)Umbelliferone2,3,4-Trihydroxybenzoic acidGuanineFA 5_1;O3LysoPC(20_1(11Z)_0_0)24-MethylenecholesterolD-Linalool 3-glucosideC16 SphinganineDesaminotyrosineGlycylleucinePentacarboxyl porphyrinogen IIIEpilubimin2-(S-Glutathionyl)acetyl chloride2',3'-Dihydro-phytomenadionePC(18_3(6Z,9Z,12Z)_20_5(5Z,8Z,11Z,14Z,17Z))TG(14_1(9Z)_22_1(13Z)_18_3(9Z,12Z,15Z))5-methylthioadenosine (MTA)De-O-methyldihydrosterigmatocystinalpha,beta-DihydroxanthohumolS-adenosylhomocysteine (SAH)6,8-Docosanedione10-OPDA(2R,3R)-DihydroflavonolAspartyl-GlutamineGibberellin A38 glucosyl ester1-deoxy-L-glycero-tetrulose 4-phosphatelupinineAdrenosteronebeta-L-RhamnoseEpipregnanoloneQuassinFA 11_13-Methoxy-4-hydroxyphenylglycolaldehydeBis(2-methyl-3-furanyl)tetrasulfide1-Phenyl-2-pentanoltrans-zeatin ribosideMG(i-18_0_0_0_0_0)LysoPE(18_2_0_0)3-Methyl-5-pentyl-2-furanheptanoic acid7-Methyluric acidGentisateLysoPC(18_0_0_0)Lysylthreonine2,4-PentanedioneTG(18_0_16_0_20_1(11Z))3-Sulfodeoxycholic acidGlycoprotein-phospho-D-mannoseAcetoacetate2-Butyl-4-propylthiazoleDihydrocucurbitacin B(1R,4S,5R)-4-ThujanolCyproteroneGlycohyocholic acidD-RiboseL,L-Cyclo(leucylprolyl)beta-CostolTrichocarposide15-Keto-prostaglandin F2abeta-Alanyl-L-lysineFurfuryl acetateFT-0670090(24R)-Ergost-4-ene-3,6-dioneRetinol(Vitamin A)Coenzyme Q9Proneurosporeneartemisinic aldehyde(1R,2R,4S)-p-Menthane-1,2,8-triol 8-glucoside8-Amino-7-oxononanoic acid5-L-Glutamyl-taurineFalcarindiol4-Methylpyrrolo[1,2-a]pyrazineAflatoxin-M1-8,9-epoxideFAL 8_16-Hydroxynicotine (S)PrednisoneIndan-1-olNigakihemiacetal BDihydroshikonofuranPrometon21-hydroxypregnenoloneButenylcarnitineST 27_2;O8RemerinTetrahydropalmatinePyruvic acidPA(18_0_16_1(9Z))6,8-HexacosanedioneN-Hydroxy-1-aminonaphthaleneL-TryptophanGlucosamine 1-phosphategibberellin A29-cataboliteDopamineAspartyl-LeucineSulochrinLycorineBenzeneethanamine, a-methyl-TamoxifenCholestenoneCyclic-3,20-bis(1,2-ethanediyl acetal)-11alpha-(acetyloxy)-5alpha,6alpha-epoxypregnane-3,20-dioneTestosterone6-(Methylsulfonyl)hexyl glucosinolate2-Ethyl-3-methylpyrazine, 9CILeukotriene B4A-MethapredParthenolide80666-03-1MyosmineEicosapentaenoic acidUracilAbietic_acidTryptamineST 27_0;O2demethylsuberosinBis(methylthio) selenideDesonidealpha-Caryophyllene alcohol acetateCreatineS-FuranopetasitinLucidine BFA 18_2;OSPB 18_2;O2NeurosporeneDihydro-O-methylsterigmatocystin4-PyridoxolactoneGalactopinitol A1-Tritriacontanol(8S,9S,10R,13S,14S,17R)-11,17-Dihydroxy-17-(2-hydroxyacetyl)-10,13-dimethyl-7,8,9,11,12,14,15,16-octahydro-6H-cyclopenta[a]phenanthren-3-one3-O-p-trans-Coumaroylalphitolic acid3,4-dihydroxy-2-methyl-4-farnesyl-3H-quinolin-1-ium-1-olate3-Methylthiopropionic acid5-Hydroxyisourate4,21-dehydrogeissoschizine5,10-Methylene-THF2-Phenoxyethyl isobutyrateAsparaginyl-ProlineTaurochenodesoxycholic acidACMC-20m7bfNorbelladineS-(Hydroxymethyl)mycothiol(E)-8-Hydroxy-2-octene-4,6-diynoic acid2,3-Dihydro-1H-pyrrolizine-5-carboxaldehyde3-Methyl-5-propyl-2-furanundecanoic acid6-Chloro-N-(1-methylethyl)-1,3,5-triazine-2,4-diamineTG(14_0_22_2(13Z,16Z)_18_3(9Z,12Z,15Z))PovpclupinateGermanicol cinnamate(S)-3-Octanol glucosidePE(18_4(6Z,9Z,12Z,15Z)_P-18_1(11Z))(ent-6alpha,7alpha)-6,7-Dihydroxy-16-kauren-19-oic acidGentamicin C2PC(16_0_18_1(11Z))Gamma-glutamyl-L-putrescineDihydropinosylvinCAR 3_0Terpendole Jpropachlor3-Mercaptolactate-cysteine disulfideCarrageenan, potassium salt ofhexestrol2-methylbutanal oximeCitronellic acidTetrahydrobiopterinVoriconazole4-Hydroxycinnamic acidSaccharopineSinapaldehyde13-HDoHEMollicellin ATyramineBerberine11,20-DihydroxysugiolPhenyl dihydrogen phosphateResolvin D2PC-M510-deacetyl-2-debenzoylbaccatin IIIMetyrapone5,6-dihydroxy-3-methylquinolin-2(1H)-oneExcoecariatoxin5,6-DHET3-(2-Methylthio)ethylmalateAgrocybinGlycerophosphocholineChlorpromazine sulfoxideErgothioneinePlatelet-activating factor7-deoxyloganetic alcoholHemigossypolLysoPC(16_0_0_0)Deoxyamidinoproclavaminate(2'E,4'Z,8E)-Colneleic acid(R)-3-(4-Hydroxyphenyl)lactate8-hydroxy-5Z,9E,11Z,14Z-eicosatetraenoic acidAfzelechinGamma-glutamyl-leucineOxymesteroneAbscisic AcidErgosta-5,7,22,24(28)-tetraen-3beta-olMarmesin6-Ketoprostaglandin E1ST 19_1;O2Asparaginegibberellin A201-Stearoylglycerophosphoserineubiquinone-8Cinnatriacetin AIndolinePhenylacetaldoximeLeukotriene A4LysoPE(20_4(8Z,11Z,14Z,17Z)_0_0)GlutamylleucineCholine phosphate7-Methylxanthine4alpha-Formyl-4beta-methyl-5alpha-cholesta-8-en-3beta-ol20, 22-DihydrodigoxigeninZolmitriptan N-Oxide3-dehydrosphinganinium(1+)AyaninExemestane1-Methylpyrrolinium3,7-Dihydroxy-12-oxocholanoic acidPC(20_1(11Z)_14_0)Cyclohexyl formateZingibereneD-ArabitolProtoporphyrin IX2-Hydroxybenzalpyruvate2-DeoxycastasteroneThromboxane B3D-(+)-dihydrocarvoneSalvigeninIsopentyl beta-D-glucosideThromboxane B2p-Cresol24-Methylenecycloartan-3-olLysoPA(20_4(8Z,11Z,14Z,17Z)_0_0)meso-Zeaxanthin11-HpODEN-MethylaspidospermatidineN-Acetyl-b-glucosaminylamineDethiobiotinApo-15-zeaxanthinalAlfentanilN-[2-Hydroxy-2-(4-hydroxyphenyl)ethyl]cinnamideEstronegamma-TocotrienolStercobilinogenPhosphoglycolic acid4-(4-Hydroxyphenyl)-2-butanone(3b,6b,8b,12a)-8,12-Epoxy-7(11)-eremophilene-6-angeloyloxy-8,12-dimethoxy-3-olDendrolasinGlutamylmethionineMetyrapolEicosapentaenoic acid ethyl esterCDPDeoxyinosineConiferyl alcoholJuvenile hormone III3-deoxy-D-manno-octulosonateMeropenem5-Amino-4-imidazole carboxylateTrans-4-hydroxyprolineHydroxyprolyl-MethionineOxoglutaric acid(2S)-2-azanyl-4-[[(2R)-3-(1H-imidazol-4-yl)-1-oxidanyl-1-oxidanylidene-propan-2-yl]amino]butanoic acidL-Proline2-MethylbutyroylcarnitineFluorocitric acidProstaglandin-c2omega-Hydroxy-beta-dihydromenaquinone-95-Aminoimidazole ribonucleotideFA 20_4;O42-Octaprenyl-3-methyl-6-methoxy-1,4-benzoquinonealpha-Tocopherol1,2,3-TrihydroxybenzeneButyrylcarnitineST 27_1;O14alpha-FormylsteroidStearoylethanolamidejuvenile hormone I5-hydroxyindole thiazolidine carboxylate4-Methylaminobutyrate5-Hydroxytryptophold-Threo biopterinPelargonidin 3-p-coumarylglucosideGlycerol 1,3-dioctadecanoate 2-(9Z-octadecenoate)Argininosuccinic acid disodium(1R)-Hydroxy-(2R)-glutathionyl-1,2-dihydronaphthalene3-Phenoxypropionic acidTrypanothione disulfideTiglylcarnitineGlucohesperalinEucalyptonePC(22_5(4Z,7Z,10Z,13Z,16Z)_18_4(6Z,9Z,12Z,15Z))DiethylphosphateEudesmic acidProtopineSinapyl alcohol1-Palmitoylphosphatidylcholine3-Sulfinylpyruvic acidDG(14_0_20_2(11Z,14Z)_0_0)7a-HydroxydehydroepiandrosteroneChrysophanol-9-anthroneProstaglandin F2alphaTetradecanoylcarnitine4-Hydroxy-3-polyprenylbenzoateBiliverdin6-Hydroxyhexan-6-olide4-Hydroxy-1-(3-pyridinyl)-1-butanone(+)-Validoxylamine-AL-Fucose(2E,6E,8E)-Decatrienoylcarnitinemethyl gibberellin A9L-argininium(1+)Serylglycine6-Oxokanamycin CEquolPhosphoribosyl formamidocarboxamideMethylprednisolone acetate3-SulfinoalanineN(4)-aminopropylspermidineBetulalbuside AEthyl apovincaminateWharanginPalmatineGeissoschizine5,7-Docosanedione(Cyclohexylmethyl)pyrazineN-[(2s)-2-Amino-2-Carboxyethyl]-L-Glutamic Acid3-hydroxy-geranylhydroquinoneL-Glutamic gamma-semialdehydeDodecanoylcarnitineHomogentisic acidL-RhamnosePC(18_1(9Z)_18_1(9Z))2-O-Methyl-L-fucose2,4-ToluenediaminePC(18_2(9Z,12Z)_18_1(9Z))Shinflavanone3-(4-Methylbenzylidene)camphorAphidicolan-16beta-ol2beta-HydroxytestosteroneGlyzarinDimethyl sulphone2-Pyrocatechuic acidPhloretin(-)-Fumigaclavine BSpermidineindole-3-acetyl-leucineAlbaconazoleLysoPE(18_0_0_0)AmantadineEmodinanthranol1,3,7-trimethylurate4-hydroxy-4-methylglutamateundecylprodigiosinPS(14_0_14_0)Dihydrocaffeic acid 3-O-glucuronideAspartylphenylalaninePC(20_4(8Z,11Z,14Z,17Z)_18_4(6Z,9Z,12Z,15Z))furaneol (keto form)MetanephrinePC(20_4(5Z,8Z,11Z,14Z)_16_1(9Z))Fructosyllysine20-Hydroxy-PGF2aEpirubicin glucuronideGamma-Glutamylvaline(+)-alpha-CareneDehydrocurdione9-HydroxyfluoreneGibberellin A108alpha-D-Glucose 1,6-bisphosphateOctadecanamideCyclohexanoneBenzimidazoleNorophthalmic acidMethyloxaloacetateAcetophenoneQuetiapineST 21_2;O4Creatinine4,4-DiapolycopenedialAcetyl-L-carnitine3,4-DichloroanilineD-XylonateAlbafuran ARosuvastatin 5 S-lactonePaspalinine2-Acetyl-4,5-dihydrothiazole3-HydroxyhexanoylcarnitineStachydrineST 29_1;O2-Amino-4-oxopentanoate7a,12a-Dihydroxy-5a-cholestan-3-oneEDDA2-Isopropylphenoltrans-2-ChlorodienelactoneHistidinalN-ethylmaleimideGalantaminechlorohexadienedioic acidDG(i-24_0_0_0_i-14_0)16beta-Hydroxysteroid2-Acetyl-6-methylpyridineMethanophenazineItaconic acidBetaineTG(16_0_18_3(9Z,12Z,15Z)_20_2n6)(13E)-11a-Hydroxy-9,15-dioxoprost-13-enoic acid15-keto PGI2Mosapramine2-Chloro-5-methyl-cis-dienelactonestylopineNorethindrone acetate5-Hydroxysulfamethoxazoleisopentenyl adenosineLotaustralin6-Imino-5-oxocyclohexa-1,3-dienecarboxylatebeta-CryptoxanthinHericenone G(3beta,17alpha,23S)-17,23-Epoxy-3,29-dihydroxy-27-norlanosta-7,9(11)-diene-15,24-dionebenzaldehydeLobelineSM(d17_1_18_0)L-LysineCapsiamideAsteroidenoneAndrostanedioneNalpha-Methylhistidine3-Hydroxyisovaleric acidDimethylbenzimidazole4alpha-formyl-ergosta-7,24(241)-dien-3beta-olIsoprenyl alcoholSM(d16_1_17_0)Glycyl-Gamma-glutamateNorethindroneisoroquefortine CSphinganineDelta-TocopherolTolbutamide2-Hydroxy-4-trifluoromethyl benzoic acidLysyltyrosineSM(d18_1_21_0)VincristineCyclomorusinm-Ethyl_toluene25-Hydroxyvitamin D2Erythromycin CNudifloramideFAL 16_12-(2,4-Dichloro-phenoxy)-N-(2-mercapto-ethyl)-acetamideStyrene cis-glycolDapsoneL-Homoserinebeta-noroxycodolGlutathionylspermidine1-(4-Hydroxyphenyl)-1-decene-3,5-dioneJubanine CN-CyclopropylammelineN(1),N(8)-bis(sinapoyl)-spermidineAnabasinePalmitoylethanolamideCiclopiroxBarbituric acidmethyl farnesoatePorphobilinogenDeisopropylatrazineAspartyl-ArginineTyrosolbeta-D-Glucose 6-phosphateTG(14_0_22_0_20_2n6)PhaseollinL-HistidinolLecithin1,3,6,8-NaphthalenetetrolAmidinoproclavaminateCoproporphyrinogen IIID-Mannose3-MethoxytyramineLinoleamideBROMOBENZENEWithaperuvin CgeranylhydroquinoneN-METHYLANILINEFADHDeoxyuridineDihydrozeatingamma-GlutamylasparagineHydroxyprolyl-ProlineN-AcetylmannosamineN1-(alpha-D-ribosyl)-5,6-dimethyl-benzimidazole2-Decylfuranpiperidine-2-carboxamide2-HydroxyacetophenoneGamma-glutamyltyrosineFragransol ALysoPE(0_0_18_0)2-(2,2,3-trimethyl-4-oxocyclopentyl)acetic acidAminocaproic acidNarbonolide5-O-Galloylhamamelofuranose8(S)-HPETEBisindolylmaleimide i(2E,5S,6E,8E,10E)-5-hydroxydodeca-2,6,8,10-tetraenalPC(18_2_18_2)PC(18_2(9Z,12Z)_16_0)N-Linoleoyl GlycineechinenoneNovapikromycinLPC 18_2Fumitremorgin Acis-Zeatin O-glucosideAcetylerioflorinFumitremorgin B9alpha-(3-Methyl-2E-butenoyloxy)-4S-hydroxy-10(14)-oplopen-3-one 4-acetate5-(Methylthio)-2,3-dioxopentyl phosphateCoumarinFA 5_0;O2SanshodiolSpermine(2S)-2-(Benzenesulfonamido)-N-[(1S)-1-hydroxy-3-methyl-1-(3-oxo-2-phenylcyclopropen-1-yl)butan-2-yl]-4-methylpentanamide2-PhenylethanolSuccinic acidLinamarinL-ArgininePC(O-18_1(11Z)_16_0)2,6-DiethylanilineArtomunoxanthentrionePhenylethylamineRishitin1-Octen-3-yl acetate2-Methylbenzaldehydep-CymeneN8-AcetylspermidineNnal-N-oxideN,N-DimethylanilineN6,N6,N6-Trimethyl-L-lysineAflatremN-acetylglucosaminylasparagine2,2'-Dithiodiethanesulfonic acid2,2,7,7-Tetramethyl-1,6-dioxaspiro[4.4]non-3-ene(9E)-9-nitrooctadecenoic Acidbeta-Apo-4-carotenalNaloxone2,2,3-trihydroxybiphenyl4-Hydroxy-3-methylbenzaldehyde2-trans,6-trans-Farnesal1-MethoxyphenanthreneEthambutol3-Ethenyl-2,5-dimethyl-4-oxohex-5-en-2-yl acetateS-Methyl-L-methionineO-acetylhomoserine(R)-carnitinamideMethylprednisolone1-(3,4-Dihydroxyphenyl)-7-(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dioneLysoPS(18_0_0_0)11beta,20-Dihydroxy-3-oxopregn-4-en-21-oic acidN-hydroxyl-tryptaminetrans-cinnamoyl-beta-D-glucosideDehydrogenated ticlopidineAnserine4alpha-Hydroxymethyl-4beta-methyl-5alpha-cholesta-8-en-3beta-ol7,8-Dihydropteroic acidVitamin A2 aldehyde5-Hydroxy-p-mentha-6,8-dien-2-one3-HydroxybenzaldehydeMethamphetamine4-Hydroxybenzoic acidCucurbitacin HGeranyl tiglateCucurbitacin PL-Methioninealpha-CEHCNaphthalenePC(O-22_0_18_3(6Z,9Z,12Z))beta-SitosterolAvocadienofuranPhentolamineTetrahydrodeoxycorticosteronePhenindione2-Acetyl-3-ethylidene-3,4,5,6-tetrahydropyridineTryptophanamide(¡À)-2-MethylbutanalGenomorphineParkeolLeucyl-leucineNSC688234Acetylenedicarboxylic acidCanadineAspartyllysineDodemorph2-cis,4-trans-xanthoxinDiisobutyl phthalateLacinilene CPrephenatePyroglutamic acidPicrocrocinPyridoxamineAurachin B8-Epidiosbulbin E acetateAndrosteroneS-AdenosylmethionineDihomolinoleate (20_2n6)FluometuronFA 12_3;O2Irisresorcinol2-Chlorooctadecanoic acid(S)-scoulerineThymidineColupuloneNonoxynol-9DiphenylamineCostunolidePhytocassane D(E)-6,10-Dimethyl-9-methylene-5-undecen-2-one24-Propylcholestan-3-olPC(P-18_0_22_0)3-ButylpyridinePalbociclibMaltose(1R)-Glutathionyl-(2R)-hydroxy-1,2-dihydronaphthaleneMethyl jasmonate4-Oxo-1-(3-pyridyl)-1-butanonePhosphoribosyl-ATP5'-DeoxyadenosineFAMPSCHEMBL2822263Lysyllysine4-Hydroxysphinganine17a-Ethynylestradiol5-Hydroxy-4-methoxy-5-(1-oxo-9,12,15-hexadecatrienyl)-2(5H)-furanoneThreonylphenylalanineAzelaic acidPC(16_0_16_1(9Z))8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)tetracenomycin C3,4-DihydroxyphenylglycolCandol BN-demethylvindolidineN2-Oxalylargininetrans-3-Chloroacrylic acidLevuglandin D2Cer(d18_1_16_0)Norvaline5-Deoxystrigoltrihydroxyferuloyl spermidineHistidylleucinefalseMetabolomic analysis of seminal fluids in oligoasthenospermia individuals<p>In this study, we systematically characterized the metabolic signatures of oligoasthenospermia individuals and health controls using high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). Additionally, we performed functional validation of specific differential metabolites in terms of their roles in enhancing sperm vitality and providing cryoprotection. This study aimed to comprehensively analyze the metabolomic signatures of seminal plasma in men with oligoasthenospermia, identify novel markers and for diagnosing and ART treating, and to provide effective strategies for improving sperm motility during in vitro manipulation in cases of oligoasthenospermia, which facilitating a deeper understanding of the mechanisms underlying male factor infertility and the protective mechanisms of sperm damage.</p>2025-07-142025-07-14MTBLS12711