MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/m_REQ20250717211868_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/m_REQ20250717211868_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/a_MTBLS12769_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/a_MTBLS12769_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/s_MTBLS12769.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12769<p>Import mass spectrometry data into Progenesis QI software to complete data alignment, grouping and peak detection. Use Progenesis QI software for metabolite identification, mainly using databases such as HMDB and KEGG.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>The chromatographic column is HSS T3 column (2.1 x 100 mm, 1.8 μm); Column temperature 40 °C; Injector temperature 10 °C; Injection volume 2 μL; Flow rate 0.40 mL/min; Mobile phase A is a 0.1% formic acid aqueous solution and mobile phase B is a 0.1% formic acid acetonitrile solution, eluted according to a gradient.</p>Exploring biomarkers for the treatment of thyroid cancer patients using multi omics techniques.Liu JunyuAffiliated Hospital of Guangdong Medical Universityfecesmass spectrometry<p>Remove the sample from liquid nitrogen and thaw it in a refrigerator at 4 °C. Take the sample and place it in a 5 mL centrifuge tube. Add 500 μL PBS and 2 Ml chloroform methanol solution, vortex for 1 min, mix well, and centrifuge at 4 °C for 10 min. Take the centrifugal sample extraction solution, use a pipette to aspirate 100 μL of the supernatant, and transfer it to injection vials with inner liners for metabolomics analysis. Use a pipette to extract 5 μL of the metabolomic analysis sample solution into the same injection vial with an inner tube, and obtain QC samples.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS12769Qin WenYi. Affiliated Hospital of Guangdong Medical University. 181039376@qq.com.<p>Firstly, handle missing values, outliers and noisy data. Then adjust the distribution of the data to make it within a unified range. Common methods include total normalization, median normalization and quantization normalization. Then perform appropriate transformations on the data, such as logarithmic transformation, to reduce the skewness of the data. Finally, scale the data to a specific range, such as mean standard deviation scaling and range scaling.</p>Treatmentlljayyu@163.com<p>This study aims to systematically collect fecal samples from thyroid cancer patients before and after treatment, storing and processing them according to stringent standardized protocols. Upon collection, samples are immediately frozen at -80°C in ultra-low temperature freezers. Subsequent processing follows aseptic operating procedures, including homogenization, DNA/RNA extraction, and quality assessment. All operations are conducted within a biosafety cabinet to ensure sample integrity and data reliability, laying the foundation for future gut microbiota metagenomic research. By comparing microbiome differences before and after treatment, this study seeks to reveal the impact of thyroid cancer therapies on gut microbial ecology.</p>Metabolomicsthyroid cancerMarkerFecesthyroid cancerMarkerFeces<p>Acquisition mode: Positive and negative ions are scanned separately (ES+/ES-), capillary voltage is 1 kV, cone hole voltage is 30 V, cone hole airflow is 50 L/h, source temperature is 150 °C, atomization temperature is 500 °C, atomization airflow is 800 L/h, acquisition mode MSE, primary voltage is 6 eV, secondary voltage is 10-50 eV, scanning range is 50-1200 m/z.</p>DG(18:1(9Z)/18:2(9Z,12Z)/0:0)[iso2]2E,4E,6Z,8Z-Decatetraenedioic acid1alpha,25-dihydroxy-19-norprevitamin D3Myxoquinone-843bacteriohopane-32,33,34,35-tetrolDG(19:1(9Z)/22:6(4Z,7Z,10Z,13Z,16Z,19Z)/0:0)[iso2]15-(3,4-dimethyl-5-pentylfuran-2-yl)-pentadecanoic acidCer(t18:1(6OH)/26:0)Tricetin 3'-sulfateLuteolin 7-(6''-E-cinnamylglucoside)Alloavicholic acidDG(16:0/22:4(7Z,10Z,13Z,16Z)/0:0)[iso2]Sarcoehrendin G14:1 Cholesterol esterN-palmitoyl methionineDG(15:1(9Z)/18:3(9Z,12Z,15Z)/0:0)[iso2]9E-pentadecenoic acid2-heptadecylenic acidNonan-2,5-dioneCholesteryl hemiazelate3-O-alpha-L-rhamnopyranosyl-3-hydroxydecanoyl-3-hydroxydecanoic acid13,14-dihydro-19R-hydroxyPGE1Tamarixetin 3-O-sulfate2alpha-(3-Hydroxypropyl)-1alpha,25-dihydroxy-19-norvitamin D3PA(18:3(9Z,12Z,15Z)/14:1(9Z))Cer(t18:0/15:0)Withasomniferol DDG(13:0/18:2(9Z,12Z)/0:0)[iso2]all-trans-7-hydroxyhexadeca-2,4,8,10-tetraenoic acidNitiducarpinDaunorubicinLinoleamideDG(22:4(7Z,10Z,13Z,16Z)/22:4(7Z,10Z,13Z,16Z)/0:0)Daturmeteside BGanoderic acid SDG(15:1(9Z)/18:1(9Z)/0:0)[iso2]Ternstroemiaxanthin5-O-Methylchamanetin2,3-dinor-5,6-dihydro-15-F2t-IsoP2,4,6-Octatriynoic acid(-)-MycomycinMannosyl-1beta-phosphomycoketide C34ThromboxaneGlcCer(t18:1(8Z)/16:0(2OH[S]))1alpha,25-dihydroxy-11alpha-[(1R)-oxiranyl]vitamin D3PG(P-16:0/20:1(11Z))36:6(5Z,9Z,12Z,15Z,18Z,21Z)(34Me[S])PrebarbigeroneVelloquercetinPI(O-16:0/13:0)Praecanson APA(22:1(11Z)/0:0)4-Hydroxy-4,4'-diaponeurosporene1alpha,22-dihydroxy-23,24,25,26,27-pentanorvitamin D3Octyl phenylacetateVeronicafolin 3-O-sulfate7alpha-methoxy-GorgosterolKurilensoside FTrichostatinPG(O-18:0/18:3(6Z,9Z,12Z))DG(15:0/22:5(7Z,10Z,13Z,16Z,19Z)/0:0)[iso2]20Z-nonacosenoic acidCer(d16:1/22:1)alpha-CortoloneKuesteriaxanthinStylissamide A30,32-dihydroxy-2b-methyl-bishomohopanePS(19:0/14:0)4,4'-DiapolycopenedialAvocadyneN-palmitoyl proline13-methyltetradecanalQuercetin 3-(2''-p-hydroxybenzoyl-4''-p-coumarylrhamnoside)Fasciculic acid B4-hydroxy-2-octenal(+)-cucurbic acidPI(21:0/13:0)22-hydroxy-23,24,25,26,27-pentanorvitamin D3Cer(t18:0/19:0)2,4,6-Octatriyn-1-oloscr#10BaicaleinSulfobacin SL3Chrysin 7-glucuronideCer(d18:2(4E,8Z)/20:0(2OH[R]))Sulfobacin SL832:6(14Z,17Z,20Z,23Z,26Z,29Z)GlcCer(d14:1(4E)/24:1(15Z)(2OH))3-Methylsubericacid20-Fluoroeicosanoic acidDG(15:0/22:4(7Z,10Z,13Z,16Z)/0:0)[iso2]Dracontioside BDehydrotestosterone2-methyl-hexadecanedioic acidCer(d16:1(4E)/20:0(2OH))Cer(d18:0/24:0)SM(d18:0/12:0)Corticrocin5-nonacosylresorcinolCladosporacid ECerP(d18:0/16:0)Clavepictine B7alpha,12alpha,24-trihydroxycholest-4-en-3-one2E,9Z-HexadecadienoylcarnitineN-docosanoyl taurineWE 6:0/16:0DG(17:0/22:5(7Z,10Z,13Z,16Z,19Z)/0:0)[iso2]1alpha,25-dihydroxy-2beta-(4-hydroxybutyl)vitamin D33-O-alpha-L-rhamnopyranosyl-3-hydroxyoctanoyl-3-hydroxydecanoic acidPA(22:0/0:0)8-tridecynoic acidIngolalpha-carboxyethylhydroxychroman sulfateN-oleoyl methionineScillirosideCrispin A6,8-Diethyl-4-methyl-3E,5E,7E,9E-dodecatetraeneFAHFA 18:1(9Z)/5O(FA 18:0)Cer(d18:0/18:0)Hexacosanoyl carnitine11-deoxy-11-methylene-15-keto-PGD2(22E)-(24R)-1alpha,24-dihydroxy-22,23-didehydrovitamin D3Tetradecyl isobutyrate27-Nor-cholestane-pentolDG(17:0/22:4(7Z,10Z,13Z,16Z)/0:0)[iso2]Patuletin 3-(6''-(E)-feruloylglucoside)Norwogonin 5,7,8-trimethyl etherN-physeteroyl lysineMicromeric acid40:6(22Z,25Z,28Z,28Z,31Z,37Z)Cer(d18:2/26:0(2OH))1-(8-[5]-ladderane-octanoyl)-2-(8-[3]-ladderane-octanyl)-sn-glycerolDG(17:1(9Z)/22:5(7Z,10Z,13Z,16Z,19Z)/0:0)[iso2]Chrysin 5-xylosideRaspailyne A1HeteranthinCer(t18:0/18:0(2OH))Macamide6-undecynoic acidCer(t15:0(14Me)/21:0(2OH[R]))Gracilosulfate BMGDG(18:2(9Z,12Z)/18:3(9Z,12Z,15Z))Gracilosulfate EGracilosulfate F8E-Heptadecenedioic acidIC202CGlisoprenin BPS-PCEcdysone 22-palmitate9,12-Octadecadienoyl carnitineSulfobacin BSulfobacin ALuteolin 3'-methyl ether 7-glucuronosyl-(1->2)-glucuronide13,14-dihydro-15-keto-PGF2alpha-d48-iso-15-keto-PGE2Japanic acidIC202AGlcCer(d18:1/24:1(15Z))(9S,13S)-10-oxo-11-phytoenoic acid6Z-Heneicosen-11-ol12-Hydroxyhexacosanoic acid26:1(5Z)(13Me,17Me,21Me,25Me)6E-Heneicosen-11-oneCer(d18:0/18:1(9Z))FAHFA 16:0/4O(FA 18:0)Itaconic acid7-Sulfocholic acidCer(d18:2/23:0)13-HODE-EA6-HydroxykaempferolGinsenoside Rh2Austrobuxusin DErucamideMuxiangrin III(+-)-DalbergioidinN-stearoyl glycine22Z-Hentriacontenoic acid(7Z,10Z)-hexadecadienoylcarnitineNemotinic acidMethoxy-keto-myxocoxanthinN-palmitoyl GABAPI(16:1(9Z)/18:0)N-palmitoyl glycineRapanonealpha-CitraurinOkenoneEpioritin-4beta-olDG(17:1(9Z)/22:4(7Z,10Z,13Z,16Z)/0:0)[iso2]1alpha,25-dihydroxy-23-oxavitamin D3(22S)-25-hydroxy-22-methoxyvitamin D3N-(3-hydroxy-octadecanoyl)-homoserine lactone1alpha,25-dihydroxy-23-azavitamin D3Diphylloside CGoniocin1alpha,25-dihydroxy-2beta-pentylvitamin D33-Tetradecenecholesterol sulfateAlbanitrile C24-hydroxy-tetracosanoic acid6Z,9Z-Nonadecadien-3-oneOphiobolin RBombycosterolMuricadieninWE 22:0/16:1(9Z)Primuletin1alpha,25-dihydroxy-3alpha-methyl-3-deoxyvitamin D33-oxohexacosanoic acid(+)-Dysideapalaunic acid11alpha-Hemiglutaryloxy-1,25-dihydroxyvitamin D3Cer(d18:1/17:0)Tricosylic acidErythromycin EPG(19:1(9Z)/17:0)PG(18:2(9Z,12Z)/18:0)2,4-decadiynoic acidN-oleoyl proline13-methoxy-heneicosanoic acid1,18-NonadecadieneCer(d20:0/16:0(2OH))DG(17:1(9Z)/18:3(9Z,12Z,15Z)/0:0)[iso2]Drophiobiolin AfalseStudy on the Changes of Fecal Lipid Metabolites in Thyroid Cancer Patients Before and After Treatment<p>Study on the Changes of Fecal Lipid Metabolites in Thyroid Cancer Patients Before and After Treatment</p>2025-07-252025-07-24MTBLS12769LMPK12130003LMGP03010624LMPK12113311LMFA13040082LMPK12020106LMFA08030043LMGL01020069LMGP06030090LMGP02010047LMGP21030005LMGP03010336LMGP01011836LMSP0504AB03LMGP02020098LMSP05010119LMFA02020007LMGP10010612LMPR0102090060LMFA01030243LMST03020049LMFA01031018LMPK15040002LMFA01140047LMFA11000050LMFA05000058LMPR0102120025LMFA05000170LMST03020477LMST01030128LMFA08020253LMFA01020136LMFA07010951LMFA01150033LMST01010554LMFA07090149LMFA01030456LMGL02010369LMST03020521LMFA01030091LMPK15010008LMPR01071053LMGL05010086LMFA01020224LMPK04000028LMSP02050003LMSP0501AA28LMSP03030150LMSP05010088LMGP06030016LMSP02010001LMFA01140033LMPR0103500002LMGL05010056LMGP02010587LMSP05010044LMFA01020001LMSP02010006LMFA07010144LMPK12020165LMGP10010183LMPK12120107LMFA11000056LMFA01050164LMFA06000089LMSP03010101LMSP02010111LMSP02030049LMGP10010474LMFA07010020LMSP02020043LMFA07010148LMFA01030830LMPK02000066LMGL03012407LMFA07011102LMSP02010139LMFA07010005LMPR01070662LMFA06000245LMFA07010169LMGP01050034LMFA07010030LMPR0103490003LMPR0102090014LMFA12000221LMFA06000104LMPR0105020006LMPK12050006LMPK12020155LMPK12112433LMST05010047LMGP02011011LMGP06010095LMSP01080033LMGP06020080LMFA11000454LMGP06020025LMPK12140069LMFA11000165LMPR0103120003LMFA01031159LMGP04010667LMPK15040006LMPR01080062LMGP04030055LMFA01020398LMPR0105050004LMFA06000061LMGP10050029LMST02020052LMFA05000119LMFA06000036LMFA01020103LMPR0103090008LMFA05000198LMPR0104140003LMFA13010047LMPR0103800001LMFA11000053LMFA06000102LMFA07090123LMFA12000272LMFA11000051LMFA11000124LMGP06020003LMGP04030021LMGL05010073LMFA01140051LMFA08040002LMFA06000034LMFA05000481LMFA06000051LMPR0103110002LMFA06000171LMFA01020107LMFA01020109LMFA01140044LMPR01070593LMFA06000024LMFA05000488LMFA01030798LMFA11000054LMFA01140056LMFA01020100LMFA06000136LMPR0102120036LMFA06000045LMFA02020009LMFA06000278LMFA11000059LMFA06000076LMFA11000696LMFA01030600LMFA06000016LMFA11000036LMPR0102090023LMFA11000061LMPR0102090016LMFA01140032LMPR0102090042LMFA11000126LMFA11000501LMFA01030588LMFA05000065LMFA01030185LMPR0102120005LMFA03010059LMFA07040157LMGL01020037LMGP04030091LMPR01070300LMFA08020223LMPR0104300001LMPR0102090022LMFA11000055LMPK13070005LMST02030306LMFA06000266LMFA03010000LMFA11000158LMFA07040002LMFA07040150LMST03020546LMST03020193LMGP23010007LMFA00000049LMGP04010088LMFA01060242LMST04030199LMST01020028LMFA01020402LMGL01020045LMST01031303LMFA11000037LMGL02010382LMFA08020184LMST02030089LMPR01070657LMST01010521LMFA01020378LMST01100009LMST04030172LMST03020592LMGP10010220LMGP04030027LMFA03010062LMFA08020219LMFA05000577LMFA08020136LMPR0106150017LMFA05000081LMST02030211LMSP00000021LMFA01050514LMFA01030852LMFA07100012LMFA05000160LMSP03030153LMST01010458LMST01010548LMPR0103060015LMFA01020336LMFA01030601LMST04010442LMGP04020024LMFA11000057LMSP02030083LMST03050001LMFA01140062LMGL05010085LMFA07070059LMST03020654LMSP05010093LMSP02010035LMSP05010091LMSP02030054LMPR01071023LMSP02020020LMSP02030051LMGP02050011LMSP02020016LMGP02010784LMFA02000180LMST05020013LMSP05010101LMSP02030052LMST01040280LMFA01030686LMGP02020044LMFA02000181LMGL02070011LMGL02010058LMFA13040068LMGL02010448LMST01060032LMFA01030842LMFA01030822LMGL02010286LMST01031205LMFA01140088LMST03020570LMST02030305LMST03020685LMSP02030090LMPR01070633LMGL02010315LMGL05010087LMSP02050004LMFA01050549LMFA02000261LMFA05000702LMGP03010943LMGP01030080LMFA01140030LMFA01050506LMST02030229LMGL01010037LMFA01050046LMST02030141LMFA07040158LMST02030200LMST04030003LMFA01030836LMFA08020270LMFA04000039LMGP04010674LMGP03010616LMGL05010082LMFA01020138LMFA01031345LMSP05010062LMFA05000200LMFA12000112LMST03020572LMGP04010718LMGL01010034LMFA05000011LMGP03010698LMFA05000216LMGL01020036LMGP03030085LMPR01070188LMGP06010282LMST04060023LMFA13010049LMFA07070040LMFA01030073LMPR0103500010LMFA01140015LMFA08020356LMPR01080064LMPR0106050018LMFA01070022LMSP02030040LMGL03012674LMST03020037LMST01100044LMGP10010524LMSP05010187LMGL03011579LMST02030316LMSP03010058LMFA06000101LMGL02010284LMST03020616LMGP06010667LMGP10010523LMGL03010980LMGP10010722LMPR0104550003LMPR02010002LMSP03010072LMGL03011176LMGP10010715LMPR01070691LMST01010371LMPR0106010044LMGL03013378LMST01020098LMGL03013444LMGL03013986LMPK12111814LMGP03010722LMGL03015408LMGL03015478LMGL03010053LMGL01010003LMGL03010194LMGL03010012LMGL03014370LMPR03040008LMGL03015172LMST01040209LMPR0103870001LMFA11000335LMFA07010826LMPR0106200004LMSP02040153LMGL03010490LMFA07090050LMPR01070849LMPR03070026LMGL03010963LMGL02010117LMPR01070696LMGL03014951LMST01040196LMFA08020190LMPK04000006LMFA07040194LMST01130006LMPR0103010003LMPK12113012LMPK12050102LMGP20010017LMPK12112794LMFA01100064LMFA02000194LMFA08020394LMFA08020392LMFA01100016LMFA06000017LMFA13010050LMGP06010673LMFA11000058LMSP0501AB23LMST03020014LMFA06000255LMGP10010198LMFA08010003LMST04010450LMFA08020121LMFA11000043LMPR0103570001LMPR0102090005LMSP02010062LMGP15010098LMFA13040017LMST03020144LMSP0501AA58LMGL02010400LMSP02030080LMGL02010320LMFA06000096LMSP0501AB11LMST03020010LMFA07010533LMST01160077LMSP05010103LMSP02030035LMFA13010010LMST01020041LMFA01060235LMSP05010055LMPR04000014LMFA01031290LMSP0501AB06LMGP06030091LMST01130017LMSP0501AC10LMGP10010752LMFA01050192LMGP06020070LMGL03013732LMFA01060095LMFA01031041LMPK12110848LMPK12110696LMST05020039LMFA01030961LMPR0103540020LMPK12050500LMPK12020188LMPK01000055LMFA03110009LMPK12110152LMFA06000039LMPK12113034LMFA01170003LMFA01050579LMFA01031139LMFA09000023LMFA01170053LMFA01170023LMPR0104310002LMPR02020083LMPK12112216LMPK12112960LMPK12111722LMFA12000244LMPK12110778LMPK12111333LMPK12140148LMST01130007LMST01010389LMFA01030614LMFA03010165LMGP20010022LMFA13040015LMFA03010154LMFA05000643LMGL01030007LMFA02020013LMGP06010179LMGP06010823LMGP04010876LMFA01031327LMGP04010488LMPR01070622LMFA02010003LMFA08020182LMFA08020079LMST05050015LMPR0105050002LMST03020063LMSP02010020LMSP0501AA69LMSP02020008LMSP05010041LMFA07010026LMPR0106050017LMPR01070605LMPK12111095LMST05020056LMST04010449LMST03020520LMSP00000023LMST01010437LMST03020047LMPR04000004LMST01060045LMFA05000535LMFA06000258LMSP00000024LMPR01070561LMST05020016LMSP00000020LMGP03010871LMSP05010128LMGL05010024LMFA01031110LMPR0104010030LMFA07010982LMST03020627LMFA01020341LMPK12070049LMFA01031036LMPK12110149LMPK13050002LMFA05000606LMPK12112283LMPK15050009LMFA01050502LMGP04020029LMST02020018LMST05020053LMFA01030981LMFA07070122LMPR0106010053LMGP04030019LMPR02030034LMPK12050072LMFA01031241LMGP10010398LMPR0106080018LMFA01090157LMST03020617LMSP01080053LMFA13030001LMFA12000206LMFA08020263LMFA08030011LMFA08040071LMGL02070019LMPR01070882LMGL02010447LMST01010439LMGL02010173LMGL02010172LMSP02020015LMPK15030014LMSP02010097LMGL02010056LMGL02010160LMGL02010545LMFA07090073LMFA07090025LMSP0501AA08LMPR01080066LMPR04000026LMSP02010030LMSP02020065LMSP02010138LMFA08020117LMFA08020109LMST04030171LMFA01030059LMFA01170036LMFA08020110LMFA08020083LMST03020411LMFA08020158LMFA13030010LMPR0106180015LMST03020343LMFA01170063LMFA08020103LMFA08010008LMGP10050031LMPK01000061LMST03020070LMPR0106010047LMFA12000211LMFA01050213LMFA01080015LMGL02010138LMGL02010298LMGL02010458LMPK03000041LMGL02010456LMFA11000670LMST01020126LMSP05010042LMSP02020035LMSP03010079LMFA07070069LMGL02010045LMPK03000102LMSP02030016LMST01020021LMSP02030033LMFA01031113LMGL02010184LMSP02010084LMST01160051LMPK12110607LMPK04000011LMPK12112860LMFA03010229LMST02030227LMPK12110099LMST05020057LMFA03010104LMST03020011LMPK12111040LMPK12120392LMPR01070602LMPR01070572LMFA08020077LMFA07010679LMSP02010049LMFA11000411LMSP02020012LMFA08020118LMFA03010179LMST01010213LMFA08010028LMST01031154LMFA07010472LMGL02010361LMFA03030015LMPR0105040001LMFA01010023LMST03020168LMFA07070021LMSP02030084LMFA01060181LMFA01150031LMFA07070009LMST03020336LMST03020501LMFA01030841LMFA01050356