MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/m_MTBLS12800_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/m_MTBLS12800_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/a_MTBLS12800_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/a_MTBLS12800_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800/s_MTBLS12800.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS12800<p>Metabolite identification were performed using Thermo Scientific Compound Discoverer 3.3 (San Jose, CA, USA) with the mass tolerance was set at 5ppm and peak intensity threshold was set at 1.5. The metabolites were identified on the basis of both accurate mass and fragment mass&nbsp;spectra via matching against the spectra of compounds available in the mzCloud, Human Metabolome Database (HMDB), and ChemSpider databases.&nbsp;</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse phaseLiquid Chromatography MS - positive - reverse phase<p>The samples were analyzed with the Thermo Scientific UltiMate 3000 UHPLC system coupled with an Orbitrap IQ-X Tribrid Mass Spectrometer (Thermo Scientific, San Jose, CA). Chromatographic separation was achieved with a Waters ACQUITY HSS T3 column (2.1 mm × 100 mm, 1.7 μm) (Milford, MA) maintained at 25 °C. The binary mobile phase consisted of (A) 0.1% formic acid in water and (B) 0.1% formic acid in acetonitrile, and the flow rate was set to 0.3 ml/min. The gradient elution (B%) started at 2% from 0 to 1 min; 35% at 5 min; 99% at 10 min and was then held until 12 min. The column was equilibrated for 3 min before the next injection. The injection volume was 3 μL.</p>Subcutaneous white adipose tissue-derived extracellular vesicle maintains intestinal homeostasis via IgA biosynthesis in aging mice.Department of Food Science and Nutrition, The Hong Kong Polytechnic UniversityPui Kei LEEblood serumSolventmass spectrometry<p>100 μL serum sample was spiked with 4-chloro- phenylalanine and ethyl gallate as the internal standards at a final concentration of 100 ng/ml. The mixture was then extracted with ice-cold 100% methanol (1 mL), centrifuged at 17,000 g at 4 °C for 15 min, and dried using a SpeedVac concentrator. The dried sample was reconstituted in 80% methanol (50 μL) followed by centrifugation at 17,000 g for 10 minutes. Quality control was prepared by pooling an aliquot of each sample together in equal amounts and 80% methanol was used as blank.</p>Mus musculusSolvent<p>Raw data were processed using Thermo Scientific Compound Discoverer 3.3 with mzCloud, Human Metabolome Database (HMDB), and ChemSpider databases.</p>Treatmentpui-kei.lee@connect.polyu.hkhttps://www.ebi.ac.uk/metabolights/MTBLS12800<p>Serum from inguinal fat-removed and sham C57BL/6J mice was collected at the endpoint and stored at -80 °C until further analysis.</p>Metabolomicsultra-performance liquid chromatography-mass spectrometrySerumuntargeted metabolitesAdipose Tissueultra-performance liquid chromatography-mass spectrometryuntargeted metabolitesSerumAdipose Tissue<p>The control sample was injected with data-dependent acquisition under both positive and negative electrospray ionization (ESI) modes to build the library of metabolites. The samples were injected with full-scan positive and negative mode. The mass scan range was 70−1200 m/z with shear gas at 45 Arb, auxiliary gas flow at 10 Arb and sweep gas flow at 2 Arb. The MS1 resolution was set at 120,000. The positive and negative ion spray voltages were 3800 V and 2300 V respectively. The ion transfer tube temperature was 300℃ and the&nbsp;vaporizer temperature was 320 ℃.</p>5,6-epoxytretinoin1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine9-cis-retinoic acidPalmitoylcarnitineall-trans-retinalLysoPC(20:5(5Z,8Z,11Z,14Z,17Z))(±)4-HydroxymephenytoinBis(2-ethylhexyl) phthalateRetinolUreidopropionic acid1-Palmitoyl-2-hydroxy-sn-glycero-3-PEophthalmic acidN-TetracosanoylglycineFenclonineLysoPC(18:3(9Z,12Z,15Z))CATECHIN GALLATE, (-)-3-Mercaptopropionic aciddihydrobiopterinThreonylserine5,6-DimethylbenzimidazolePYROQUILONfalseSubcutaneous white adipose tissue-derived extracellular vesicle maintains intestinal homeostasis via IgA biosynthesis in aging mice<p>To identify the potential iWAT-derived adipokines that regulate intestinal IgA biosynthesis, we conducted untargeted metabolomics in the serum of iFR and iSham mice. A total of 214 differential abundant metabolites were identified (log2FC &gt;1 or &lt;-1 and adjusted p-value &lt;0.05) between the two groups. Pathway analysis showed that retinol metabolism was the most significantly altered pathway by iWAT removal. Despite retinol level remained unchanged, its downstream oxidized products, including retinal, RA, and all-trans-5,6-epoxy-RA, were significantly downregulated in the iFR mice. The reduction of RAs was further confirmed through using a targeted LC-MS/MS analysis.&nbsp;</p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