Lipid identification was achieved through a spectral match using LipidBlast library, which was developed using R and based on XCMS.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive"],"chromatography_protocol":["LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific), equipped with a Kinetex C18 column (2.1 mm x 100 mm, 1.7 μm, Phenomen). The mobile phase A consisted of 40 % water, 60 % acetonitrile, and 10 mmol/L ammonium formate. The mobile phase B consisted of 10 % acetonitrile and 90 % isopropanol, which was added with 50 mL 10 mmol/L ammonium formate for every 1000 mL mixed solvent. The analysis was carried with elution gradient as follows: 0-1.0 min,40 % B;1.0-12.0 min,40 %-100 % B; 12.0-13.5 min, 100 % B; 13.5-13.7 min, 100 %-40 % B; 13.7-18.0 min, 40 % B. The column temperature was 55 °C. The auto-sampler temperature was 4 °C, and the injection volume was 2 μL (positive) or 2 μL (negtive), respectively.
"],"publication":["Zinc oxide nanoparticles supplementation improve the quality and lipid profiles of boar frozen semen."],"submitter_affiliation":["Anhui Agricultural University"],"submitter_name":["Kangmin Li"],"organism_part":["semen"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The samples were transferred with 250 μL of water. After 30 s vortex, the samples were freezed and thawed with liquid nitrogen for 3 times. Then 50 μL was taken out for protein determination. For each remaining sample, 480 μL MTBE: MEOH=5:1 was added. After 30 s vortex, then the samples were sonicated for 10 min in ice-water bath. Then the samples were incubated at -40 °C for 1 h and centrifuged at 3000 rpm for 15 min at 4 °C. 300 μL of supernatant was transferred to a fresh tube and dried in a vacuum concentrator at 37 °C. Then, the dried samples were reconstituted in 200 μL of 50% methanol in dichloromethane by sonication on ice for 10 min. The constitution was then centrifuged at 12000 rpm for 15 min at 4 °C, and 100 μL of supernatant was transferred to a fresh glass vial for LC/MS analysis.
"],"organism":["Sus scrofa domesticus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS12892"],"author":["Yongqi Chen.","Kangmin Li. Anhui Agricultural University. 1026007691@stu.ahau.edu.cn."],"data_transformation_protocol":["The raw data files were converted to files in mzXML format using the msconvert program from ProteoWizard. The CentWave algorithm in XCMS was used for for peak detection, extraction, alignment, and integration, the minfrac for annotation was set at 0.5, the cutoff for annotation was set at 0.3.
"],"study_factor":["Status"],"submitter_email":["1026007691@stu.ahau.edu.cn"],"sample_collection_protocol":["Four adult Huoshou black boars were housed under standardized husbandry practices: regulated environmental temperature, consistent dietary protocols, a 16-h light cycle that combines natural and artificial illumination, and unlimited access to potable water. The fraction of ejaculate with high gamete density was collected from the boars using a gloved-hand technique: a trained technician donned sterile latex gloves, initiated manual stimulation of the animal’s penile tissue, carefully grasped the spiral glans upon the boar’s erection, and after discarding the initial 10–15 mL (which contained a low density of the target gametes), this high-density fraction was transferred into a sterile vessel preheated to 37 °C. Each boar had semen collected twice weekly, with three of their ejaculates set aside for freezing sperm. Ejaculate samples were obtained from each individual boar on two separate occasions per week. From every collection, three ejaculates were allocated for sperm cryopreservation. Each animal underwent a total of eight collections over the course of the study. For the purposes of this research, 'semen' stands for the entire ejaculate, which includes spermatozoa and seminal plasma, whereas 'sperm' indicates only spermatozoa that have been separated from seminal plasma. For the freezing process, only top-quality semen, defined by a milky white color, freedom from any foreign particles or unusual smells, and a motility rate of over 80%, was chosen. The freezing process involved two stages (21): semen from different Huoshou black boars was first pooled for the purpose of reducing interanimal variability. Then, an appropriate amount of ZnONPs (pig frozen semen dilution kit purchased from Beijing Yuantian Aori Company) was added to semen dilution solutions I and II. Qualified semen samples (whole ejaculates) were first proportionally diluted with predilution solutions and transferred to a 17 °C controlled environment for preliminary cooling, which lasted approximately 4 h. After the sample achieved equilibrium at 17 °C, the ejaculate was subjected to centrifugation using a large-capacity centrifuge at 800 × g for a 15-minute period. The seminal plasma fraction (the resulting supernatant) was removed and discarded, and the concentrated pellet of the reproductive cells was quickly resuspended in a commercially sourced cryopreservation medium (provided by Green Auris, Beijing, China). This resuspended ejaculate preparation was placed in a pre-adjusted 17 °C water bath prior to being transferred to a temperature-controlled environment held at 4 °C for a secondary cooling step; this phase persisted for approximately 4 h. Once equilibrium was achieved at 4 °C, isothermal cryoprotection solution (Green Auris, Beijing, China) consisting of solution I (265–280 mL), glycerol (15–30 mL), and surfactant (mass fraction 0.1–0.4%) was added to the semen to achieve equal dilution. The main components of Solution I are glucose (22–30 g), citric acid (2.5–4.5 g), sodium citrate (4–8 g), HEPES sodium (2–3.2 g), Tris (4–7 g), EDTA-Na2 (1.5–4 g), sodium bicarbonate (0.5–2 g), cysteine (0.01–0.2 g), pure water (1000 mL), and yolk fluid (250 mL) were added to 0.5 mL cryogenic straws (Minitube, Munich, Germany), which were sealed with sealing powder. After the semen was filled into the straws, the thin tubes were placed on the tube rack, and after placing them, they were placed in a foam container filled with liquid nitrogen for fumigation. The distance between the thin tubes and the surface of the liquid nitrogen was 3 cm. The semen was frozen and stored in liquid nitrogen for at least 2 months before being used in the study.
"],"omics_type":["Metabolomics"],"study_design":["gas chromatography-mass spectrometry","untargeted metabolites","Sus scrofa domesticus"],"curator_keywords":["gas chromatography-mass spectrometry","untargeted metabolites","Sus scrofa domesticus"],"mass_spectrometry_protocol":["The Thermo Scientific Q Exactive HF-X mass spectrometer was used, with data-dependent acquisition (DDA) mode controlled by Thermo Xcalibur 4.3 software. In DDA mode, the full scan MS spectrum was continuously evaluated, and the top 10 most intense ions were selected for MS/MS fragmentation (dynamic exclusion duration: 30 s). ESI source conditions were set as follows: sheath gas flow rate: 30 instrument-specific arbitrary units (Arb); aux gas flow rate: 10 Arb; capillary temperature: 320 °C (positive mode), 300 °C (negative mode); full MS resolution: 70000 at m/z 200; MS/MS resolution: 17500 at m/z 200; collision energy: 15, 30, 45 eV (stepped NCE mode); scan m/z range: 100-1000; spray voltage: 5 kV (positive mode) or -4.5 kV (negative mode).
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2/16:2/19:0)","TAG(15:2/15:2/15:2)","DGTS(2:0/16:2)","PC(16:0e/22:6)","ACar(15:1)","TAG(16:0/18:0/18:2)","TAG(12:1/19:5/19:5)","PE(14:0/20:4)","TAG(18:4/18:4/19:0)","TAG(15:0/15:0/22:1)","LPE(20:1)","PC(12:0/22:3)","Cer/NS(d18:1/22:2)","AcylGlcADG(14:0/14:0/20:4)","PC(7:0/26:1)","PC(20:5/22:6)","PE(18:2/16:3)"],"additional_accession":[]},"is_claimable":false,"name":"Zinc oxide nanoparticles supplementation improve the quality and lipid profiles of boar frozen semen","description":"Oxidative stress frequently causes a reduction in the quality of frozen-thawed sperm. Zinc oxide nanoparticles (ZnONPs) has an antioxidant protective effect on frozen semen of animals such as goats, camels and buffaloes. However, it is not clear whether ZnONPs can improve the quality and lipid composition of frozen semen in pigs. In this study, the frozen semen of Huoshou black boars was divided into three groups, and different concentrations of zinc oxide nanoparticles (0 mg/ml, 0.05 mg/ml, 0.1 mg/ml) were added to the semen of Huoshou black boars. The results showed that the sperm motility and kinematic parameters of the experimental group incubated with 0.1 mg/mL ZnONPs at 37℃ for 0 h and 1 h were significantly higher than those of the control group (P < 0.05). The quality of the frozen semen after incubation at 37℃ for 0 h and 1 h with 0.1 mg/mL ZnONPs was detected. The results showed that the sperm motility, plasma membrane integrity, and total antioxidant capacity (T-AOC) of the experimental group were significantly higher than those of the control group (P < 0.05), while the level of reactive oxygen species (ROS) was significantly lower than that of the control group (P < 0.05). In addition, the frozen semen with 0.1 mg/mL ZnONPs was used as the experimental group, and the frozen semen without 0.1 mg/ ml ZnONPs was used as the control group. The results showed that there were 74 differential lipid molecules in the experimental group compared with the control group in the positive ion mode, of which 8 were significantly up-regulated and 66 were significantly down-regulated. The results showed that the addition of 0.1mg/ml ZnONPs could promote the preservation of porcine semen.
","dates":{"publication":"2026-05-05","submission":"2025-08-25"},"accession":"MTBLS12892","cross_references":{}}