This study aims to detect the bile acid profile in fecal samples and identify specific bile acid types by comparing them with bile acid reference standards.
soft JhengH"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse phase"],"chromatography_protocol":["This project employs a Waters ACQUITY I-Class ultra-high-performance liquid chromatography system. Chromatographic separation of target compounds is achieved using an ACQUITY UPLC BEH C18 1.7μm column (1.7μm, 2.1 × 100 mm, Waters). LC mobile phase A consists of 0.1% formic acid in water containing 5 mM ammonium acetate, while phase B is acetonitrile:methanol = 3:1. The column oven temperature is set to 35°C, the sample tray to 10°C, and the injection volume to 2 μL.
"],"publication":["Integrative analysis of gut microbiota, bile acid pathways, and immune dysregulation in dyslipidemia models."],"submitter_name":["Jiayue Xia"],"submitter_affiliation":["southeast university"],"organism_part":["feces"],"technology_type":["mass spectrometry"],"disease":[""],"extraction_protocol":["Weigh an appropriate amount of sample into a 2 mL centrifuge tube, add steel balls, and add 1 mL of acetonitrile:methanol:water solution (2:2:1, V/V/V). Vortex for 30 seconds. Homogenize at 25 Hz for 10 min, followed by ultrasonic extraction in an ice-water bath for 10 min and vortexing for 5 min. Centrifuge the sample at 4°C, 12,000 rpm for 10 min. Finally, transfer 500 μL of the supernatant through a 0.22 μm filter membrane for injection and analysis. Quality control samples must be included concurrently with the test samples.
"],"organism":["Rattus norvegicus domestica"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS13092"],"author":["Jiayue Xia. southeast university. School of Public Health, Southeast University, Nanjing, China. 230229010@seu.edu.cn."],"data_transformation_protocol":["Differential bile acids were identified through a univariate statistical analysis, with statistical significance defined as a P-value less than 0.05. All mass spectrometric data acquisition and quantitative analysis of target compounds were performed using SCIEX Analyst Workstation Software (Version 1.7.2) and Sciex OS 2.0.1.
"],"study_factor":["Treatment"],"submitter_email":["230229010@seu.edu.cn"],"sample_collection_protocol":["Seven-week-old SD rats were fed either a standard diet containing 10% of energy from fat or a high-fat diet containing 40% of energy from fat for 18 weeks. Fecal samples were collected and stored at -80°C for subsequent determination of the bile acid profile in the fecal samples.
"],"omics_type":["Metabolomics"],"study_design":["Dyslipidemia","Feces","targeted metabolites","Bile Acid Measurement"],"curator_keywords":["Dyslipidemia","Feces","targeted metabolites","Bile Acid Measurement"],"mass_spectrometry_protocol":["This project utilizes a SCIEX 6500 QTRAP+ triple quadrupole mass spectrometer equipped with an IonDrive Turbo V ESI ion source for mass spectrometry analysis in multiple reaction monitoring (MRM) mode. Ion source parameters are as follows: Curtain Gas = 35 psi, IonSpray Voltage = -4500V, Temperature = 550°C, Ion Source Gas 1 = 50 psi, Ion Source Gas 2 = 55 psi.
"],"metabolite_name":["Dehydrolithocholic acid"],"additional_accession":[]},"is_claimable":false,"name":"Integrative analysis of gut microbiota, bile acid pathways, and immune dysregulation in dyslipidemia models_Bile acid profile of rat fecal samples","description":"This study aims to compare changes in bile acid profiles between fecal samples from rats fed a standard control diet and those fed a high-fat diet.
","dates":{"publication":"2025-10-04","submission":"2025-10-04"},"accession":"MTBLS13092","cross_references":{}}