Metabolite information was retrieved from the Human Metabolome Database (HMDB).
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS -"],"chromatography_protocol":["For sip53 and corresponding control samples, liquid chromatography was performed on a Shimadzu Nexera UHPLC system coupled to a QTRAP 6500+ mass spectrometer (AB Sciex) equipped with an IHILIC-(P) Classic column (5 µm, 150 × 2.1 mm, 200 Å; Hilicon). Mobile phase A consisted of 20 mM ammonium acetate and 25 mM ammonium hydroxide, and mobile phase B was acetonitrile. The flow rate was set to 0.4 mL/min. The gradient was programmed as follows: 0–26 min, 85% B, 32% B, 2% B, and 85% B. The mass spectrometer operated in multiple-reaction monitoring mode with electrospray ionization at 475 °C; ion source gas 1 and 2 were both 60 psi, curtain gas was 35 psi, and the spray voltage was 4,500 V.
For siLAMP2A and corresponding controls, LC–MS analysis was carried out on a Shimadzu Nexera UHPLC system interfaced with a TripleTOF 6600+ mass spectrometer (AB Sciex). Chromatographic separation employed an IHILIC-(P) Classic column (5 µm, 150 × 2.1 mm, 200 Å; Hilicon) using mobile phase A (20 mM ammonium acetate, 0.1% ammonium hydroxide, and 2.5 µM methylene diphosphate in 95:5 water/acetonitrile) and mobile phase B (acetonitrile). The gradient program was: 0 min, 85% B; 2 min, 85% B; 7 min, 65% B; 12 min, 35% B; 12.1 min, 20% B; 15.9 min, 20% B; 16 min, 85% B; 23 min, 85% B. The flow rate was 0.2 mL/ min, the injection volume 5 µL, and the total run time 23 min. Electrospray ionization settings were: GS1 = 60, GS2 = 60, CUR = 35, temperature = 500 °C, and ion spray voltage = +5000 V or −4500 V for positive and negative modes, respectively.
"],"publication":["p53 regulates Chaperone-mediated autophagy."],"submitter_name":["Yi Xu"],"submitter_affiliation":["Fudan University"],"organism_part":["Cell Line"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Cells were extracted using ice-cold solvent (acetonitrile:methanol:water, 40:40:20, v/v/v) containing 0.5% formic acid, and the acid was subsequently neutralized with ammonium bicarbonate. The extracts were transferred to 1.5-mL Eppendorf tubes and stored overnight at −80 °C. The samples were then centrifuged at 13,000 × g for 15 min at 4 °C to remove precipitated proteins, and the resulting supernatants were collected for metabolomics analysis.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS13190"],"author":["Yi Xu. Fudan University. yi_xu@fudan.edu.cn."],"data_transformation_protocol":["Raw LC–MS/MS data files were first converted to mzML format using MSConvert (ProteoWizard). The resulting mzML files were processed with the XCMS package in R for peak detection, alignment, and peak table generation. Peak intensities were normalized to total protein concentration prior to downstream analysis.
"],"study_factor":["Treatment"],"submitter_email":["yi_xu@fudan.edu.cn"],"sample_collection_protocol":["U2OS cells were transfected with control, p53, or LAMP2A siRNA for 48 hours. Cells were then extracted using ice-cold solvent (acetonitrile:methanol:water, 40:40:20, v/v/v) containing 0.5% formic acid, and the acid was subsequently neutralized with ammonium bicarbonate. The resulting extracts were stored at −80 °C until analysis.
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","chaperone-mediated autophagy","p53"],"curator_keywords":["Metabolomics","chaperone-mediated autophagy","p53"],"mass_spectrometry_protocol":["LC–MS analysis for p53 siRNA and corresponding control siRNA groups was performed on a Shimadzu UHPLC system coupled to a triple quadrupole mass spectrometer (QTRAP 6500+, AB Sciex). The mass spectrometer was operated in multiple reaction monitoring (MRM) mode with an electrospray ionization (ESI) source set at 475 °C. The ion source gas pressure was 60 psi, curtain gas 35 psi, and the spray voltage 4,500 V.
LC–MS analysis for LAMP2A siRNA and corresponding control siRNA groups was carried out on a Shimadzu UHPLC system coupled to a TripleTOF mass spectrometer (QTOF 6600+, AB Sciex) equipped with an electrospray ionization (ESI) source operating in both positive and negative ion modes. The ESI parameters were as follows: GS1 = 60, GS2 = 60, CUR = 35, temperature = 500 °C, and ion spray voltage = +5000 V or −4500 V for positive and negative modes, respectively.
"],"metabolite_name":["dGDP","Taurine","D-Fructose","Allantoin","p-Cresol sulfate","Glucaric acid","Riboflavin","5-Thymidylic acid","O-Phosphoethanolamine","CDP","Folic acid","L-Homoserine","Fructose 1,6-bisphosphate","Thiamine pyrophosphate","D-Xylose","gamma-Aminobutyric acid","Deoxyinosine","D-Ribose 5-phosphate","2-Hydroxyethanesulfonate","L-Threonine","8-Aminooctanoic acid","Guanosine diphosphate","Kynurenic acid","beta-Alanine","Ketoleucine","L-Histidine","L-Leucine","Pyridoxal","Thiamine monophosphate","Oxoglutaric acid","alpha-Ketoisovaleric acid","Biotin","Glucosamine-1P","L-Proline","Urea","Flavin mononucleotide","Octadecanedioic acid","4-Hydroxyproline","Glucosamine","N2-Acetylornithine","Thymidine 5'-triphosphate","trans-Aconitic acid","Pyruvic acid","Carnosine","Butyrylcarnitine","Glycine","Penicillin G","L-Pipecolic acid","N-Acetylglutamine","L-Asparagine","N6-Acetyl-L-lysine","Guanosine monophosphate","Leukotriene C4","L-Tryptophan","Glutathione","Niacinamide","S-Ribosylhomocysteine","Deoxyuridine","Uridine diphosphate-N-acetylglucosamine","Methylcysteine","Phenylacetylglycine","5-Hydroxylysine","4-Guanidinobutanoic acid","Prolylhydroxyproline","LysoPC(18:0/0:0)","octulose 8-phosphate","15(R)-hydroperoxy-EPE","L-Valine","Uracil","N-Acetylneuraminic acid","Pantothenic acid","4-Pyridoxic acid","Glycerol 3-phosphate","N-Acetyl-L-methionine","L-Kynurenine","Glyceraldehyde 3-phosphate","Creatine","ADP","N2-gamma-Glutamylglutamine","Inosinic acid","Phytosphingosine","IDP","D-2-Hydroxyglutaric acid","Alanylglutamine","Shikimate-3-phosphate","Cholesterol sulfate","Phthalic acid","L-Cystine","N6-Methyladenosine","Aminoadipic acid","Deoxyribose 5-phosphate","Cyclic AMP","2-Aminoisobutyric acid","alpha-Hydroxyisobutyric acid","2,4-Di-tert-butylphenol","Oxidized glutathione","Methylguanidine","Spermine","Myristic acid","Succinic acid","Uridine","PC(32:0)","octose 1,8-bisphosphate","Hypoxanthine","Phenyllactic acid","Ornithine","N-Acetylaspartylglutamic acid","NADPH","L-Arginine","Hippuric acid","2-Hydroxyglutarate","Indole","Alpha-Lactose","3-Sulfinoalanine","dTDP","Isocitric acid","L-Cysteine","L-Carnitine","N6,N6,N6-Trimethyl-L-lysine","L-Lactic acid","2-Hydroxybutyric acid","Thiamine","Adenosine triphosphate","L-Glutamic acid","2,4-Dihydroxypyrimidine-5-carboxylic acid","p-Octopamine","Adenosine","Adenosine monophosphate","N-Acetyl-L-glutamic acid","Homocitrulline","Adenine","Threonic acid","Acetyl-CoA","Uric acid","Acetylphosphate","2-Hydroxyphenethylamine","Guanidoacetic acid","LysoPC(16:0/0:0)","NAD","Fumaric acid","L-Tyrosine","Heptadecanoic acid","Citraconic acid","D-Erythrose 4-phosphate","L-Serine","Inosine","Argininosuccinic acid","Homo-L-arginine","Homocysteine","3-Hydroxybutyric acid","Lumichrome","Dimethylglycine","1,2-Dipalmitoyl-rac-glycero-3-phosphoethanolamine","L-Phenylalanine","Ascorbic acid","Glycyl-glycine","Dihydroxyacetone phosphate","Cholic acid","Palmitic acid","Glycylproline","Sedoheptulose 1,7-bisphosphate","O-Acetylserine","4-Trimethylammoniobutanoic acid","Bicine","Spermidine","Anserine","Uridine diphosphate glucose","Ureidosuccinic acid","Hexahydropyrrolo[1,2-a]pyrazine-1,4-dione","N-alpha-Acetyl-L-lysine","Tyramine","Folinic acid","N-Formyl-L-methionine","Deoxyadenosine","D-Xylitol","3-Indolebutyric acid","L-Methionine","Ribitol","Acetaminophen","dCTP","L-Cystathionine","Glycerophosphocholine","Methylhistidine","Tryptophanamide","1-Aminocyclopropanecarboxylic acid","Guanine","Aminomalonic acid","Levoglucosan","5'-Methylthioadenosine","Diisobutyl phthalate","NADH","1-Methylnicotinamide","(2R,3R,4S,5R)-2-amino-3,4,5,6-tetrahydroxyhexanal","myo-Inositol","Mannitol","Pyroglutamic acid","Glucose 1-phosphate","Cytidine triphosphate","NADP","2-Keto-L-gluconate","D-Galactose","Creatinine","Phosphoenolpyruvic acid","Citicoline","L-Acetylcarnitine","N-Acetylputrescine","Xanthosine","Pyridoxine","Xanthine","Uridine 5'-monophosphate","Glycylleucine","S-Adenosylmethionine","6-Phosphogluconic acid","Citric acid","Fructose 6-phosphate","N-Acetyl-glucosamine 1-phosphate","D-Sedoheptulose 7-phosphate","Guanosine triphosphate","L-Glutamine","Quinic acid","Uridine diphosphate glucuronic acid","Choline","N-Acetylleucine","N-Acetyl-L-alanine","Acetylcholine","N-a-Acetyl-L-arginine","Hydroxyisocaproic acid","Galactose 1-phosphate","PS(18:1(9Z)/18:1(9Z))","Cytidine monophosphate","Nicotinic acid","Guanosine diphosphate mannose","Thymidine","Glycocholic acid","L-Isoleucine","Glyceric acid 1,3-biphosphate","5-Methyltetrahydrofolic acid","Methylmalonic acid","Glucose 6-phosphate","Betaine","L-Alanine","Asymmetric dimethylarginine","Malic acid","Cysteine-S-sulfate","3-Phosphoglyceric acid","Leucylalanine","CDP-ethanolamine","Gluconic acid","4-Acetamidobutanoic acid","2-Hydroxycaproic acid","Deoxyuridine triphosphate","2-Hydroxy-3-methylbutyric acid","Phosphorylcholine","S-Adenosylhomocysteine","Cytidine","LysoPC(17:0/0:0)","N-Acetyl-L-aspartic acid","2-Hydroxy-2-methylbutyric acid","Symmetric dimethylarginine","L-Aspartic acid","2-Phenylpropionate","L-Lysine","Isovalerylglycine","Trimethylamine N-oxide","Methionine sulfoxide","Phthalic anhydride","Chenodeoxycholic acid glycine conjugate","3-Hydroxyisovaleric acid","Allantoic acid","3,4-Dihydroxyhydrocinnamic acid","1-Methylhistidine","Glyoxylic acid","Taurodeoxycholic acid","Trehalose 6-phosphate","Uridine 5'-diphosphate","cis-Aconitic acid","Uridine triphosphate","Deoxyadenosine monophosphate","Guanosine 3',5'-bis(diphosphate)","Thymine","N-Acetylasparagine","Phosphocreatine","Tartaric acid","Deoxycholic acid","DL-O-Phosphoserine","6-O-phosphonato-D-glucono-1,5-lactone(2-)","D-Ribose","Carbamoyl phosphate","Sarcosine","dUMP"],"additional_accession":[]},"is_claimable":false,"name":"p53 regulates Chaperone-mediated autophagy","description":"p53 protects against the initiation and progression of a plethora of cancers, but the mechanisms underlying this critical function remain incompletely understood. In addition, chaperone-mediated autophagy (CMA) is major form of autophagy unique to vertebrates, yet both its regulation and function are unclear. Here, we demonstrate that p53 acts as a central activator of CMA, integrating diverse stress signals to regulate this highly selective autophagic pathway. Mechanistically, AMPK—a key cellular energy sensor—inhibits CMA by abrogating the oligomerization of the CMA receptor LAMP2A. Cytoplasmic p53 interacts with AMPK and relieves its inhibitory effect. By activating CMA, p53 targets isocitrate dehydrogenases IDH1 and IDH2 for degradation, inhibiting reductive conversion of glutamine to citrate and suppressing oncogenic growth. Tumor-associated p53 mutants lack the CMA-promoting activities, while oncogenic IDH mutants are resistant to p53- and CMA-mediated degradation. These results suggest that activation of CMA may be a fundamental component of p53 biological function, through which it modulates metabolism and inhibits oncogenic growth.
","dates":{"publication":"2026-10-03","submission":"2025-10-21"},"accession":"MTBLS13190","cross_references":{"HMDB":["HMDB0000001","HMDB0000008","HMDB0000011","HMDB0000012","HMDB0000017","HMDB0000019","HMDB0000030","HMDB0000033","HMDB0000034","HMDB0000043","HMDB0000044","HMDB0000045","HMDB0000050","HMDB0000052","HMDB0000056","HMDB0000058","HMDB0000062","HMDB0000064","HMDB0000071","HMDB0000072","HMDB0000082","HMDB0000086","HMDB0000089","HMDB0000092","HMDB0000094","HMDB0000095","HMDB0000097","HMDB0000098","HMDB0000099","HMDB0000101","HMDB0000112","HMDB0000119","HMDB0000121","HMDB0000123","HMDB0000124","HMDB0000125","HMDB0000126","HMDB0000128","HMDB0000132","HMDB0000134","HMDB0000138","HMDB0000143","HMDB0000148","HMDB0000156","HMDB0000157","HMDB0000158","HMDB0000159","HMDB0000161","HMDB0000162","HMDB0000167","HMDB0000168","HMDB0000172","HMDB0000175","HMDB0000177","HMDB0000182","HMDB0000186","HMDB0000187","HMDB0000190","HMDB0000191","HMDB0000192","HMDB0000193","HMDB0000194","HMDB0000195","HMDB0000201","HMDB0000202","HMDB0000206","HMDB0000208","HMDB0000210","HMDB0000211","HMDB0000214","HMDB0000217","HMDB0000220","HMDB0000221","HMDB0000224","HMDB0000230","HMDB0000235","HMDB0000239","HMDB0000243","HMDB0000244","HMDB0000251","HMDB0000254","HMDB0000262","HMDB0000263","HMDB0000267","HMDB0000271","HMDB0000273","HMDB0000283","HMDB0000285","HMDB0000286","HMDB0000288","HMDB0000289","HMDB0000290","HMDB0000292","HMDB0000294","HMDB0000295","HMDB0000296","HMDB0000299","HMDB0000300","HMDB0000306","HMDB0000407","HMDB0000423","HMDB0000446","HMDB0000450","HMDB0000462","HMDB0000479","HMDB0000508","HMDB0000510","HMDB0000517","HMDB0000538","HMDB0000562","HMDB0000564","HMDB0000574","HMDB0000606","HMDB0000619","HMDB0000625","HMDB0000626","HMDB0000634","HMDB0000637","HMDB0000640","HMDB0000641","HMDB0000645","HMDB0000653","HMDB0000660","HMDB0000663","HMDB0000670","HMDB0000678","HMDB0000679","HMDB0000684","HMDB0000687","HMDB0000695","HMDB0000696","HMDB0000699","HMDB0000714","HMDB0000715","HMDB0000716","HMDB0000719","HMDB0000721","HMDB0000725","HMDB0000729","HMDB0000731","HMDB0000738","HMDB0000742","HMDB0000746","HMDB0000754","HMDB0000759","HMDB0000765","HMDB0000766","HMDB0000779","HMDB0000782","HMDB0000806","HMDB0000807","HMDB0000812","HMDB0000821","HMDB0000828","HMDB0000883","HMDB0000895","HMDB0000896","HMDB0000902","HMDB0000905","HMDB0000925","HMDB0000929","HMDB0000935","HMDB0000939","HMDB0000943","HMDB0000956","HMDB0000958","HMDB0000960","HMDB0000996","HMDB0000998","HMDB0001015","HMDB0001031","HMDB0001058","HMDB0001065","HMDB0001067","HMDB0001068","HMDB0001096","HMDB0001109","HMDB0001112","HMDB0001124","HMDB0001138","HMDB0001147","HMDB0001161","HMDB0001163","HMDB0001173","HMDB0001185","HMDB0001191","HMDB0001198","HMDB0001201","HMDB0001206","HMDB0001209","HMDB0001227","HMDB0001256","HMDB0001257","HMDB0001270","HMDB0001273","HMDB0001274","HMDB0001316","HMDB0001321","HMDB0001325","HMDB0001341","HMDB0001342","HMDB0001367","HMDB0001372","HMDB0001396","HMDB0001397","HMDB0001401","HMDB0001406","HMDB0001409","HMDB0001413","HMDB0001473","HMDB0001487","HMDB0001488","HMDB0001494","HMDB0001511","HMDB0001514","HMDB0001520","HMDB0001522","HMDB0001539","HMDB0001545","HMDB0001546","HMDB0001548","HMDB0001562","HMDB0001564","HMDB0001565","HMDB0001586","HMDB0001624","HMDB0001721","HMDB0001859","HMDB0001906","HMDB0001987","HMDB0002005","HMDB0002013","HMDB0002064","HMDB0002096","HMDB0002107","HMDB0002108","HMDB0002259","HMDB0002666","HMDB0002917","HMDB0003011","HMDB0003072","HMDB0003334","HMDB0003335","HMDB0003337","HMDB0003357","HMDB0003464","HMDB0003681","HMDB0003903","HMDB0004044","HMDB0004610","HMDB0004620","HMDB0004825","HMDB0006028","HMDB0006029","HMDB0006695","HMDB0010382","HMDB0010384","HMDB0011635","HMDB0011727","HMDB0011732","HMDB0011733","HMDB0011738","HMDB0011743","HMDB0011745","HMDB0011756","HMDB0012108","HMDB0012390","HMDB0013318","HMDB0013816","HMDB0013835","HMDB0015186","HMDB0028685","HMDB0028922","HMDB0036458","HMDB0059638","HMDB0059655","HMDB0060274","HMDB0062295","HMDB0062628","HMDB0246972","HMDB0247418","HMDB0253141","HMDB0254199","HMDB0256501","HMDB0258272"]}}