The metabolites were identified by accuracy mass and MS/MS data which were matched with HMDB (http://www.hmdb.ca), massbank (http://www.massbank.jp/), KEGG (https://www.genome.jp/kegg/), LipidMaps (http://www.lipidmaps.org), mzcloud (https://www.mzcloud.org) and the metabolite database bulid by Panomix Biomedical Tech Co., Ltd. (Shuzhou, China). The molecular weight of metabolites was determined according to the m/z (mass-to-charge ratio) of parent ions in MS data. Molecular formula was predicted by adduct ion, and then matched with the database to realize MS identification of metabolites. At the same time, the MS/MS data from quantitative table of MS/MS data, were matched with the fragment ions and other information of each metabolite in the database, so as to realize the MS/MS identification of metabolites.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - positive","Liquid Chromatography MS - negative"],"chromatography_protocol":["The LC analysis was performed on a Vanquish UHPLC System (Thermo Fisher Scientific, USA). Chromatography was carried out with an ACQUITY UPLC ® HSS T3 (2.1× 100 mm, 1.8 µm) (Waters, Milford, MA, USA). The column maintained at 40°C. The flow rate and injection volume were set at 0.3 mL/min and 2 μL, respectively. For LC-ESI (+)-MS analysis, the mobile phases consisted of (B2) 0.1% formic acid in acetonitrile (v/v) and (A2) 0.1% formic acid in water (v/v). Separation was conducted under the following gradient: 0-1 min, 8% B2; 1-8 min, 8%-98% B2; 8-10 min, 98% B2; 10-10.1 min, 98%-8% B2; 10.1-12 min, 8% B2. For LC-ESI (-)-MS analysis, the analytes were carried out with (B3) acetonitrile and (A3) ammonium formate (5mM). Separation was conducted under the following gradient: 0-1 min, 8% B3; 1-8 min, 8%-98% B3; 8-10 min, 98% B3; 10-10.1 min, 98%-8% B3; 10.1-12 min, 8% B3.
"],"publication":["Bifidobacterium animalis subsp. lactis V9 Overcomes CYFRA 21-1-Linked Immunochemotherapy Resistance in NSCLC via Microbial Metabolites."],"submitter_name":["Cuijiao Feng"],"submitter_affiliation":["inner mongolia agricultural university"],"organism_part":["serum"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Chemicals and reagents:
LC-MS grade methanol (MeOH) was purchased from Fisher Scientific (Loughborough, UK). 2-Amino-3-(2-chloro-phenyl)-propionic acid was obtained from Aladdin (Shanghai, China).
Instruments
High speed freezing centrifuge was obtained from Hunan Xiangyi Experiment Equipment Co., Ltd. (Hunan, China). Vortex mixer was obtained from Haimen Kylin-bell Lab Instruments Co., Ltd. (Haimen, China). Ultrasonic cleaner was obtained from Kunshan Shumei Experiment Equipment Co., Ltd. (Kunshan, China). Tissue grinder was obtained from Zhejiang Meibi Experiment Equipment Co., Ltd. (Zhejiang, China). Microporous membrane filters (0.22 µm) were purchased from Tianjin Jinteng Experiment Equipment Co., Ltd. (Tianjin, China).
Sample preparation:
1. Accurately weigh an appropriate amount of sample into a 2 mL centrifuge tube, add 600 µL MeOH (Containing 2-Amino-3-(2-chloro-phenyl)-propionic acid (4 ppm), vortex for 30 s;
2. Add steel balls, placed in a tissue grinder for 120 s at 50 Hz;
3. Room temperature ultrasound for 10 min;
4. Centrifuge for 10 min at 12,000 rpm and 4℃, filter the supernatant by 0.22 μm membrane and transfer into the detection bottle for LC-MS detection.
"],"organism":["Mus musculus","Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS13300"],"author":["Cuijiao Feng. inner mongolia agricultural university. 18829285357@163.com."],"data_transformation_protocol":["Data preprocessing
The raw data were firstly converted to mzXML by MSConvert in ProteoWizard software package (v3.0.8789) and processed using R XCMS (v3.12.0) for feature detection, retention time correction and alignment. Key parameters settings were set as follows: ppm = 15, peakwidth = c (5, 30), mzdiff = 0.01, method = centWave. The batch effect was then eliminated by correcting the data based on QC samples. Metabolites with RSD > 30% in QC samples were filtered and then used for downstream analysis.
"],"study_factor":["Treatment"],"submitter_email":["18829285357@163.com"],"sample_collection_protocol":["The origin of sample comes from Homo sapiens or Mus musculus serum with no any treatment and have only one timepoint
"],"omics_type":["Metabolomics"],"study_design":["Fecal","untargeted metabolites","Serum","non-small cell lung carcinoma"],"curator_keywords":["Fecal","untargeted metabolites","Serum","non-small cell lung carcinoma"],"mass_spectrometry_protocol":["Mass spectrometric detection of metabolites was performed on Orbitrap Exploris 120 (Thermo Fisher Scientific, USA) with ESI ion source. Simultaneous MS1 and MS/MS (Full MS-ddMS2 mode, data-dependent MS/MS) acquisition was used. The parameters were as follows: sheath gas pressure, 40 arb; aux gas flow, 10 arb; spray voltage, 3.50 kV and -2.50 kV for ESI(+) and ESI(-), respectively; capillary temperature, 325°C; MS1 range, m/z 100-1000; MS1 resolving power, 60000 FWHM; number of data dependant scans per cycle, 4; MS/MS resolving power, 15000 FWHM; normalized collision energy, 30%; dynamic exclusion time, automatic.
"],"metabolite_name":["2-Furoate","Saikosaponin A","NCGC00381061-01_C30H51N5O9_Pyrrolo[1,2-d][1,4,7,10,13,16]oxapentaazacyclononadecine-1,4,7,10,14,17(11H,16H)-hexone, 16-(2,3-dihydroxypropyl)dodecahydro-5,8,9,21-tetramethyl-6-(1-methylethyl)-3-(1-methylpropyl)-","Phenylacetylglutamine","Methionine sulfoximine","NCGC00381248-01!4-oxododecanedioic acid","Diazoxide","Docosatetraenoic acid","NP-008198","NP-021171","L-Erythrulose","Ergonovine","Andrographiside","Ketoleucine","3,5,7,10,15-O-pentacetyl-14-O-benzoyl-10,18-dihydromyrsinol","Prostaglandin E2 p-benzamidophenyl ester","Reserpine","Tacrine","Phenazepam","1-({(2R,3S,4R,5S)-3,4-Dihydroxy-5-[2-oxo-2-(4-phenyl-1-piperazinyl)ethyl]tetrahydro-2-furanyl}methyl)-3-isopropylurea","Tetracosanoic acid","4,5-DIDEMETHYLSIMMONDSIN","2,2',4,4'-Tetrahydroxybenzophenone","Grayanotoxin I","Lutein","4,15,26-trihydroxy-1-oxa-4,9,15,20,26,31-hexazacyclotritriacontane-5,8,16,19,27,30-hexone","NP-015723","Carprofen","phorbol-12,13-didecanoate","Licoricesaponin H2","N1-[4-(trifluoromethoxy)phenyl]acetamide","Hemin","Neopterin","Fluroxypyr","4-Hydroxybenzaldehyde","BIX01294","N-Oleoyl-L-Serine","Agnuside","7(S),17(S)-dihydroxy-8(E),10(Z),13(Z),15(E),19(Z)-Docosapentaenoic Acid","TOFA","Perfluorooctanoic acid","NCGC00381117-01!2-amino-4-hydroxy-5-[[1-(4-methoxy-6-oxopyran-2-yl)-3-methylbutyl]amino]-5-oxopentanoic acid","6beta-Hydroxytestosterone","3-(2,3-dihydro-1,4-benzodioxin-6-ylamino)-2-[3-(trifluoromethyl)benzoyl]acrylonitrile","NP-003964","Methoprene acid","1D-Myo-inositol 1,4,5,6-tetrakisphosphate","NCGC00385653-01_C36H40O8_","12,13-DHOME","Latanoprost (free acid)-d4","12b-O-[deca-2E,4Z-dienoyl]-13a-isobutyl-4b-phorbol","2,4,12-Octadecatrienoic acid isobutylamide","FMN","4,4'-Sulfonylbis[2-(prop-2-en-1-yl)phenol]","16-phenoxy tetranor PGF2alpha methyl amide","Lamivudine","NCGC00179905-02!(E)-3-(4-methoxyphenyl)-1-[2,4,6-trimethoxy-3-(3-methylbut-2-enyl)phenyl]prop-2-en-1-one","Fexofenadine","alpha-Ergocryptine","4,4'-Sulfonyldiphenol","Sulfisoxazole","Fluocinonide","3',5'-Cyclic dAMP","16-Oxopalmitate","3-(2-Hydroxyphenyl)propanoic acid","Ritodrine","ISOXICAM","NCGC00180261-02!1,11,21-trihydroxy-1,6,11,16,21,26-hexazacyclohentriacontane-2,5,12,15,22,25-hexone","N-Acetylneuraminic acid","Pantothenic acid","N1-cyclohexyl-2-(4-pyridylcarbonyl)hydrazine-1-carbothioamide","PI(18_1_13-HODE)","Glycerol 3-phosphate","Vindoline","Indoxylsulfuric acid","Yohimbine","Ergocorninine","3-Indoleacrylate","4-Hydroxycinnamyl alcohol 4-D-glucoside","Shikimic acid","NP-017710","Etomidate","Malabaricano","NCGC00380717-01_C27H36O7_2-Butenoic acid, 2-methyl-, 9a-(acetyloxy)-1a,1b,4,4a,5,7a,7b,8,9,9a-decahydro-7b-hydroxy-3-(hydroxymethyl)-1,1,6,8-tetramethyl-5-oxo-1H-cyclopropa[3,4]benz[1,2-e]azulen-9-yl ester, (2E)-","NCGC00381146-01!2-[5-[2-[2-[5-(2-oxopropyl)oxolan-2-yl]propanoyloxy]butyl]oxolan-2-yl]propanoic acid","Vicriviroc Malate","NCGC00380181-01_C41H66O15_1-O-[(2beta,3beta,5xi,9xi,18xi)-2,23,29-Trihydroxy-28-oxo-3-(beta-D-xylopyranosyloxy)olean-12-en-28-yl]-beta-D-glucopyranose","6-Azuridine","Kanamycin","NP-022068","Crustecdysone","Phthalic acid","NCGC00380711-01_C32H44O12_Propanoic acid, 2-methyl-, (3S,3aR,4R,6S,7R,10E,12R,13aR)-3,4,6,13a-tetrakis(acetyloxy)-2,3,3a,4,5,6,7,8,9,12,13,13a-dodecahydro-2,9,9,12-tetramethyl-5-methylene-8,13-dioxo-1H-cyclopentacyclododecen-7-yl ester","NCGC00385692-01_C44H68O14_3-Hydroxyspirosta-5,25(27)-dien-1-yl 2-O-(6-deoxy-alpha-L-mannopyranosyl)-4-O-(2-hydroxy-3-methylpentanoyl)-alpha-L-arabinopyranoside","Prostaglandin D3","Mupirocin","2-[6-(diethylamino)purin-9-yl]-5-(hydroxymethyl)oxolane-3,4-diol","Isorhamnetin","L-NIL","Digitoxin","L-NIO","PC(16_1_10-HDoHE)","Biotin-XX hydrazide","Dihydro Isorescinnamine","Chenodeoxycholic acid","NCGC00381054-01!(2E,4E)-12-[(10E,12E)-13-carboxy-3-[(2E,4E)-13-carboxy-12,14-dihydroxy-3,5,7-trimethyltetradeca-2,4-dienoyl]oxy-2-(hydroxymethyl)-8,10,12-trimethyltrideca-10,12-dienoyl]oxy-13-(hydroxymethyl)-3,5,7-trimethyltetradeca-2,4-dienedioic acid","2-(4-hydroxy-1,3-thiazol-2-yl)-1-phenylethan-1-one","NCGC00380387-01_C21H27NO11_","Cycloaspeptide A_130056","Lovastatin","SANGUINARINE SULFATE","Glucobrassicin","4-[(1R,9S)-3-(2-Fluorophenyl)-6-oxo-7,11-diazatricyclo[7.3.1.02,7]trideca-2,4-dien-11-yl]-4-oxobutanoic acid","LSM-4394","7-Hydroxy-2-(4-hydroxyphenyl)-4-oxo-3,4-dihydro-2H-chromen-5-yl ?-D-glucopyranoside","Sulfasalazine","3alpha,7alpha-Dihydroxy-12-oxo-5beta-cholanate","NP-002676","Avocadene 2-acetate","NP-000256","5,7-O-Diacetyl-14-O-benzoyl-3-O-propanoylmyrsinol","Adenosine","NP-004626","3',5'-Dichloro-2-hydroxy-2-methylbut-3-enanilide","Pifithrin-&mu","NCGC00180855-02_C23H36O4_2-Pentenoic acid, 3-[(acetyloxy)methyl]-5-[(1R,4aR,8aR)-decahydro-5,5,8a-trimethyl-2-methylene-1-naphthalenyl]-, methyl ester, (2Z)-","N-(2,1,3-Benzoxadiazol-4-yl)-2-(propylsulfanyl)nicotinamide","N-(2-Cyanoethyl)-N'-[3,5-bis(trifluoromethyl)phenyl]-N-tetrahydrofuran-2-ylmethylthiourea","NCGC00386096-01!(9E,15E,19E)-3-(2-amino-2-oxoethyl)-11,13-dihydroxy-14,21-dimethoxy-8,10,12-trimethyl-7,21-dioxohenicosa-9,15,19-trienoic acid","Prostaglandin B1","Uric acid","Indolepyruvate","Inosine-5'-triphosphate trisodium salt","2,5-di-tert-Butylhydroquinone","Digalacturonate","D-Erythrose 4-phosphate","Homo-L-arginine","5-aminonaphthalene-2-sulfonic acid","2,3-Dinor-8-iso prostaglandin F2alpha","Sarmentoside B","20-Carboxy-leukotriene B4","Melatonin","Daidzin","indigo carmine","Stearidonic acid","(2E,6E,11E,13E)-18-(2,6-dioxopiperidin-4-yl)-9-hydroxy-8-methoxy-10,12,14-trimethyl-15-oxooctadeca-2,6,11,13-tetraenoic acid","Sisomicin","NCGC00347776-02!3-chloro-6-hydroxy-2-(4-hydroxy-2-methoxy-6-methoxycarbonylphenoxy)-4-methylbenzoic acid","Tetradecanedioic acid","(2S)-N2-[(2S)-1-amino-3-cyclohexyl-1-oxo-2-propanyl]-N1-isopropyl-N4-phenyl-1,2,4-piperazinetricarboxamide","N-Acetyl-D-galactosamine","Aripiprazole","LYSYL-TYROSYL-LYSINE","Naloxone HCl","4-[3-(5-Oxo-2,3-dihydro-5H-pyrimido[2,1-b][1,3]thiazol-6-yl)-1,2,4-oxadiazol-5-yl]butanoic acid","Ecgonine","NP-018606","O1-(2-Thienylcarbonyl)-3-[(2-pyridylthio)methyl]benzene-1-carbohydroximamide","Calcimycin","D-Glyceraldehyde 3-phosphate","4alpha-deoxyphorbol 12-tiglate-13-isobutyrate (4-dPE A)","Mizolastine","NCGC00180087-02!5-(hydroxymethyl)-3-(1-hydroxy-4-methylhexyl)oxolan-2-one","Octabenzone","2-Ketobutyric acid","Gibberellin A3","1,3,5-trihydroxy-4-{[(2E)-3-(3-hydroxy-4-methoxyphenyl)prop-2-enoyl]oxy}cyclohexane-1-carboxylic acid","FAHFA 26_0","MLS000859974-01!LY-165,163","NCGC00386014-01![(2S,3R,4S,5S,6R)-4,5-dihydroxy-6-[(4-hydroxybenzoyl)oxymethyl]-2-(4-hydroxyphenoxy)oxan-3-yl] 3,4,5-trihydroxybenzoate","Nitrofurazone","Buprenorphine","_130047","laccaic acid A","2-[(1-ethyl-1H-1,2,3,4-tetraazol-5-yl)sulfanyl]-1-(4-phenylpiperazino)-1-ethanone","Cymoxanil","NCGC00380208-01!bis[[4-[(2S,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyphenyl]methyl] 3-hydroxy-3-methylpentanedioate","2-(N-Methyl-perfluorooctane sulfanamido) acetic acid","Phenobarbital-d5","Uridine diphosphategalactose","Quinmerac","Roxithromycin (Roxl-150)","Rifampicin","Gancaonin A","N-Acetyl-L-carnosine","fipronil-sulfide","D-Galactose","2-hydroxy-4-methoxy-3,5-bis(3-methylbut-2-enyl)-6-pentylbenzoic acid","2',5'-Dihydroxychalcone","2-[(7-chloro-4-quinazolinyl)oxy]-N-[3-(trifluoromethyl)phenyl]acetamide","Iprovalicarb","dGMP","PS(18_0_11,12-EET)","NCGC00384643-01_C20H32O2_(2E)-3-Methyl-5-[(1S,8aS)-5,5,8a-trimethyl-2-methylenedecahydro-1-naphthalenyl]-2-pentenoic acid","4-(acetylamino)phenyl 6-(trifluoromethyl)pyridine-2-carboxylate","(15Z)-9,12,13-Trihydroxy-15-octadecenoic acid","Chloraminophenamide","Citric acid","milbemycin A4","NCGC00385498-01!3-[[12-acetyloxy-17-(2,5-dihydroxy-6-methylhept-6-en-2-yl)-17-hydroxy-4,4,8,10,14-pentamethyl-1,2,3,5,6,7,9,11,12,13,15,16-dodecahydrocyclopenta[a]phenanthren-3-yl]oxy]-3-oxopropanoic acid","NCGC00381349-01_C11H13NO5_1H-Pyrrole-3-propanoic acid, 5-acetyl-4-(carboxymethyl)-","Etidronic acid","Suspensaside","CAY10570","Verapamil","(3beta,9xi)-3-(beta-D-Glucopyranosyloxy)-14-hydroxycard-20(22)-enolide","5-{[(5-chloro-2-pyridyl)amino]methylidene}-2,2-dimethyl-1,3-dioxane-4,6-dione","N'2-[3-(trifluoromethyl)benzoyl]-5-(trifluoromethyl)pyridine-2-carbohydrazonamide","NCGC00385436-01!","3,3'-Diisopropyl-6,6'-dimethyl-2,2',5,5'-biphenyltetrol","Milrinone","alpha-D-Ribose 1-phosphate","Pravastatin","Glycocholic acid","Zingerone","Fenoldopam","Tolytoxin","NCGC00384699-01!(2E,6E,10E)-3,7,11,15,19,23,27,31,35-nonamethylhexatriaconta-2,6,10,34-tetraene-1,15,19,23,27,31-hexol","Tetrabromobisphenol A","Erythromycin estolate","(2R)-2-Hydroxy-3-(phosphonatooxy)propanoate","5-PAHSA","7-Dehydrocholesterol","Hydroflumethiazide","2,6-Dimethoxy-1,4-benzoquinone","NP-003145","Val-Phe4Cl-OH","Quassin","Flurecol","N'1,N'3-bis(2-Thienylmethylidene)-2-butylpropanediohydrazide","Anandamide","2,5-Dimethylphenol","CAY10599","Monobutyl phthalate","Colchicine","Ethyl N?[({4?[(1E)?2?{4?[(1E)?2?(2,5?dimethoxyphenyl)diazen?1?yl]?2,5?dimethoxyphenyl}diazen?1?yl]?3?methylphenyl}(ethyl)amino)methyl]carbamate","Palmitoyl 3-carbacyclic Phosphatidic Acid","12b-O-[non-2Z,4E-dienoyl]-13_-isobutyroyloxy-7-oxo-5-ene-4b-phorbol","3-Oxalomalate","Genistein","Chenodeoxycholic acid glycine conjugate","5-Methoxyindoleacetate","Roxithromycin","NP-015117","Propylthiouracil","SB 216763","N-[4-(3-Chloro-4-methylphenyl)-5-methyl-1,3-thiazol-2-yl]-1-phenyl-5-propyl-1H-pyrazole-4-carboxamide","perfluorohexanoic acid","Norbenzoylecgonine","3-(3,4-Dihydroxy-5-methoxy)-2-propenoic acid","Thyrotropin releasing hormone","7-DESHYDROXYPYROGALLIN-4-CARBOXYLIC ACID","1-O-(1,3,23,24,25-Pentahydroxy-28-oxo-9,19-cyclolanostan-28-yl)hexopyranose","NCGC00180451-03!(3S,4S)-5-[(3S,4S)-4,10-dihydroxy-7,9-dimethoxy-3-methyl-3,4-dihydro-1H-benzo[g]isochromen-5-yl]-7,9-dimethoxy-3-methyl-3,4-dihydro-1H-benzo[g]isochromene-4,10-diol","NP-007966","N1-[4-(3-pyrazin-2-yl-4,5-dihydro-1H-1,2,4-triazol-5-yl)phenyl]acetamide","EPA (d5)","(S)-Abscisic acid","3,5-Diiodothyronine","11b-hydroxypristimerin","4-Hydroxy-3- methoxyphenylglycol sulfate","N-dodecanoyl-L-Homoserine lactone-3-hydrazone-biotin","NCGC00385198-01_C37H50O9_","NCGC00380713-01_C21H35NO6S_Cysteine, S-[(6E,9E)-5-hydroxy-8-methoxy-5,9,13,14-tetramethyl-2-oxooxacyclotetradeca-6,9-dien-4-yl]-","(Z)-2-tetracos-15-enamidoethanesulfonic acid","Succinyl sulfathiazole","1-Methyluric acid","Pelargonic acid","ginsenoside C-K","{3,4,5-triacetyloxy-6-[5-acetyloxy-3-(4-methoxyphenyl)-4-oxochromen-6-yloxy]-2 H-3,4,5,6-tetrahydropyran-2-yl}methyl acetate","methyl 9-(2,4-dimethoxyanilino)-9-oxononanoate","3,5-Diiodo-L-tyrosine","NCGC00168874-03!N-[3-[5,8-bis[3-[acetyl(hydroxy)amino]propyl]-3,6,9,12,15,18-hexaoxo-1,4,7,10,13,16-hexazacyclooctadec-2-yl]propyl]-N-hydroxyacetamide","Ethyl myristate","Sinomenine","minecoside","Cytokinin B","Met-Phe4Cl-OH","NCGC00380197-01!2-[[2-(1,3-dicarboxypropyl)-6,6,9a-trimethyl-5-[(2E,4E,6E)-octa-2,4,6-trienoyl]oxy-1-oxo-4,5,5a,7,8,9-hexahydro-3H-benzo[e]isoindol-4-yl]amino]pentanedioic acid","NP-020521","fumonisin B4","p-Cresol sulfate","Arachidic acid","Luteolin-6-C-arabinoside-8-C-glucoside","Leukotriene F4","NCGC00386086-01![9-[(E)-3-acetyloxy-4-methylhex-4-en-2-yl]-12-hydroxy-3-(2-hydroxypropan-2-yl)-10-methoxy-6a,12b-dimethyl-8,11-dioxo-1,2,3,4a,5,6,12,12a-octahydropyrano[3,2-a]xanthen-1-yl] acetate","PGPC","O-Phosphotyrosine","NP-004031","Procymidone","Alpha-dimorphecolic acid","N'-(cyclohexylcarbonyl)-4-methyl-1,2,3-thiadiazole-5-carbohydrazide","Nelfinavir","NP-010966","NCGC00347544-02!4-hydroxy-3-[(E)-7-hydroxy-3,7-dimethyl-4-oxooct-5-enyl]-5-(3-methylbut-2-enyl)benzoic acid","NP-005128","Proscillaridin","3-Hydroxyvalproic acid","Felodipine","Myristoleic acid","4-Aminophenol","(-)-Guttiferone E","Robustic acid","N-(3-oxododecanoyl) homoserine lactone","Falcarindiol","NP-020515","8-Hydroxyguanosine","Ganoderic acid K","2,4-DB","2-Aminoflubendazole","Glutamylglutamic acid","Saccharin sodium anhydrous","5-(2,3-dihydro-1H-inden-5-yl)-2-methyl-7-(trifluoromethyl)pyrazolo[1,5-a]pyrimidine","Rutarensin","PE(18_1(9Z)_0_0)","Fucose 1-phosphate","Salicin","3-O-p-hydroxybenzoyl ceanothic acid","NCGC00386015-01_C32H44O8_Estra-1,5-diene-3,11-dione, 17-[(1R,3E)-1-(acetyloxy)-5-hydroxy-1,5-dimethyl-2-oxo-3-hexen-1-yl]-2,16-dihydroxy-4,4,9,14-tetramethyl-, (9beta,10alpha,16alpha,17beta)-","DDMS","10E,12Z-Octadecadienoic acid","dTMP","2-Benzothiazolesulfonic acid","CAPSO","Glycerophospho-N-Oleoyl Ethanolamine","clevidipine","6k-PGF1alpha-d4","2-Naphthalenesulfonic acid","Cryptotanshinone","Calcitriol","NP1EC (TENTATIVE)","Medicarpin","Fraxidin","NCGC00347713-02_C36H44N8O9_Cyclo(glycyltryptophylprolylglycylvalylglycyl-beta-hydroxytyrosyl)","2,4-dihydroxyheptadec-16-enyl acetate","dTDP-D-glucose","Leukotriene C4","Gentisic acid","NCGC00169202-02!5-chloro-2,4-dihydroxy-6-methyl-3-[(2E,4E)-3-methyl-5-[(1R,2R,6R)-1,2,6-trimethyl-3-oxocyclohexyl]penta-2,4-dienyl]benzaldehyde","Paclitaxel","Adouetine X","Teraspiridole A_130090","(plusmn)-Mevalonic acid 5-pyrophosphate tetralithium salt","SB 939","Penicillin V","Amygdalin","13,14-Dihydro-15-keto Prostaglandin A2","Dopamine","5'-Uridylic acid, 2'-deoxy-, disodium salt","N-Acetyl-beta-alanine","NP-016234","2,3-DIACETOXY-7,8-EPOXY-24,29-DINOR-1,3,5-FRIEDELATRIENE-20-CARBOXYLIC ACID","NP-004020","2-Methoxyestradiol","4-Acetyl-3-hydroxy-5-methylphenyl ?-D-glucopyranoside","NCGC00380949-01_C28H42O5_3,5,9,23-Tetrahydroxy-18,22-cycloergosta-6,8(14)-dien-11-one","Leukotriene C4 methyl ester","methyl 5-(tert-butyl)-3-{[(4-chlorophenyl)sulfonyl]methyl}thiophene-2-carboxylate","2-methyl-3-Pyrimidin-2-yl-Propionic Acid","Uracil","Levocetirizine","Chloroxynil","Palmitoleoyl 3-carbacyclic Phosphatidic Acid","Methyl 4-{[1,3-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)-2-propanyl]oxy}-3-methoxybenzoate","11-Dehydrocorticosterone","Iodotyrosine","NP-016067","LysoPA(16_0_0_0)","diuron-desmethyl","3,5,7,8,15-pentaacetoxy-2-hydroxy-9,14-dioxojatropha-6(17),11E-diene","N2-gamma-Glutamylglutamine","Fragransin B2","conocarpan","6-Hydroxy-2-naphthoic acid","IDP","N1-isopropyl-2-(7-oxabicyclo[2.2.1]hept-2-ylcarbonyl)hydrazine-1-carbothioamide","NCGC00169882-02_C14H20O4_(8aR,12S,12aR)-12-Hydroxy-4-methyl-4,5,6,7,8,8a,12,12a-octahydro-2H-3-benzoxecine-2,9(1H)-dione","4-oxo-4-[(3-{4-[4-(trifluoromethyl)pyrimidin-2-yl]piperazino}propyl)amino]butanoic acid","NCGC00380425-01!1-(3,5-dihydroxyphenyl)-12-hydroxytridecan-2-one","Cholesterol sulfate","PC(18_0_10-HDoHE)","2-{5-[2-({2-[5-(2-hydroxybutyl)oxolan-2-yl]propanoyl}oxy)propyl]oxolan-2-yl}propanoic acid","N'3-(2,2-dimethylpropylidene)-1-(4-isopropylphenyl)-5-methyl-1H-pyrazole-3-carbohydrazide","NP-009525","N-(1-Amino-1-oxo-2-propanyl)-1,3,4-trihydroxy-5-{[(4-methylphenyl)carbamoyl]amino}cyclohexanecarboxamide","3-[(4-chlorobenzoyl)amino]propanoic acid","N3-(4-chlorophenyl)-4-(trifluoromethyl)nicotinamide","12b-O-[non-2E,4Z-dienoyl]-13_-methylbutyroyloxy-4b-phorbol","NP-019562","Bexarotene","L-Cystine","2-({[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]imino}methyl)phenol","N2,N2-Dimethylguanosine","Etamycin","N-[3-(aminosulfonyl)phenyl]-2,3-dihydro-1,4-benzodioxine-2-carboxamide","Guanidinosuccinic acid","CAY10626","Isocarbophos","Indole-3-acetyl-L-valine","Doramapimod","(3S,5R,6R,7E)-3,5,6-Trihydroxy-7-megastigmen-9-one","NCGC00386062-01!(3S,6R,12S)-6-benzyl-3-butan-2-yl-9-(6,7-dihydroxyoctyl)-1,4,7,10-tetrazabicyclo[10.4.0]hexadecane-2,5,8,11-tetrone","1-Deoxy-D-xylulose 5-phosphate","Phosphatidylinositol-1,2-dipalmitoyl","Selenomethionine","Adrenic acid","NADPH","NCGC00178802-07!N-[5-[[4-[5-[acetyl(hydroxy)amino]pentylamino]-4-oxobutanoyl]-hydroxyamino]pentyl]-N'-(5-aminopentyl)-N'-hydroxybutanediamide","3-Methylthiopropionic acid","Ethirimol","4-Chloro-3-sulfamoylbenzoic acid","Cyclanilide","NCGC00169536-02!2,3-dihydroxypropyl 3-hydroxytetradecanoate","cucurbitacin I 2-O-beta-D-glucopyranoside","Taurochenodesoxycholic acid","3-(1-cyano-1,2-dihydroisoquinolin-2-yl)-3-oxopropyl propionate","NCGC00386076-01_C19H33N3O5_2,8-Diisobutyl-5-methyl-1-oxa-4,7,10-triazacyclotetradecane-3,6,9,14-tetrone","(1S)-1,5-Anhydro-1-[2,4,6-trihydroxy-3-(4-hydroxybenzoyl)phenyl]-D-glucitol","PS(18_0_14,15-EpETE)","N-({(2R,4S,5R)-5-[3-(3,4-Dimethoxyphenyl)-1-methyl-1H-pyrazol-5-yl]-1-azabicyclo[2.2.2]oct-2-yl}methyl)-2,2-dimethylpropanamide","Heptenophos","1-{[(2R,4S,5S)-5-{[Allyl(methyl)amino]methyl}-1-azabicyclo[2.2.2]oct-2-yl]methyl}-3-[4-(trifluoromethoxy)phenyl]urea","Methylgingerol","(+_-)-Gingerol","Xanthopterin-B2","Catechin","L-902,688","NP-000947","Cilastatin","5,6,7-triacetoxy-3-benzoyloxy-14,15-dihydroxy-9-oxojatropha-6(17),11E-diene (8)","NP-019547","EP_M424","5-KETE","CAY10408","1,4-Dihydroxy-2-naphthoate","NP-005397","ethyl-2-amino-4-methyl-Thiazole-5-Carboxylate","NCGC00380378-01![(2R,3R,4S,5S,6R)-3-acetyloxy-2-(acetyloxymethyl)-5-hexanoyloxy-6-[(2S,3R)-2,3,4-trihydroxybutoxy]oxan-4-yl] 14-hydroxytetradecanoate","3,5-dichlorosalicylic acid","Palmitoleic acid","NCGC00380235-01_C32H48O9_beta-D-Xylopyranoside, 3,17-dihydroxyspirosta-5,25(27)-dien-1-yl","N-(3-Methoxypropyl)-2-{2-[(2,3,4,5,6-pentamethylbenzyl)thio]acetyl}hydrazine-1-carbothioamide","Heptadecanoic acid","Doxycycline","2-methoxy-5-(1H-1,2,4-triazol-5-yl)-4-(trifluoromethyl)pyridine","Repaglinide","Myriocin-12-en","RUTILANTINONE","7-{[(2E)-3,7-Dimethyl-2,6-octadien-1-yl]oxy}-1,3-dihydroxy-9H-xanthen-9-one","NCGC00381167-01!(E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enamide","Corchorifatty acid F","3-Sialyl-N-acetyllactosamine","Phenylacetic acid","Triclabendazole","Sumatriptan","TG (14_0_16_1(9Z)_14_0)-d5","Caproic acid","Lacosamide","6-hydroxy-7-methyoxyscytophycin E","Telaprevir","Neu5Gcalpha2-3Galbeta1-4Glcbeta-Sp","5,7-diacetoxy-3-benzoyloxy-14,15-dihydroxy-8-(2-methylbutyryloxy)-9-oxojatropha-6(17),11E-diene","Palmitic acid","NP-001608","(3beta,5xi,9xi)-23-Hydroxy-3-{[beta-D-xylopyranosyl-(1-3)-6-deoxy-alpha-L-mannopyranosyl-(1-2)-alpha-L-arabinopyranosyl]oxy}olean-12-en-28-oic acid","delta-Tocotrienol","(Z)-5,8,11-trihydroxyoctadec-9-enoic acid","Adenosine 3',5'-diphosphate","bekanamycin sulfate","(plusmn)4-HDoHE","Diethylstilbestrol","4-Hydroxycinnamic acid","8002-43-5","(2S)-7-Hydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl ?-D-glucopyranoside","Cefadroxil","3-(hexopyranosyloxy)-2-hydroxypropyl (9Z,12Z,15Z)-octadeca-9,12,15-trienoate","Spongiacidin C","NCGC00380876-01_C29H38O14_Methyl 1,4-bis(beta-D-glucopyranosyloxy)-3-(3-methyl-2-buten-1-yl)-2-naphthoate","2-(Methylsulfonyl)-3-(pyrazin-2-ylamino)acrylonitrile","1-{[(2R,4S,5R)-5-{[(2R)-2-(Methoxymethyl)-1-pyrrolidinyl]methyl}-1-azabicyclo[2.2.2]oct-2-yl]methyl}-3-phenylurea","Meloxicam","Berberine","2-Amino-3-phosphonopropionic acid","Lys-Gln-Ala-Gly-Asp-Val","PC(16_0e_10-HDoHE)","Esculin","Elevated serum cytokeratin 19 fragment (CYFRA 21-1) marks aggressive disease
and poor response to immunochemotherapy in advanced non-small cell lung cancer
(NSCLC), but its link to gut microbiome dysfunction remains unclear. We show that
high CYFRA 21-1 levels correlate with depletion of Bifidobacterium animalis and
antitumor metabolites. Gut microbial metabolites from these patients suppress
immunochemotherapy efficacy in mice through dysregulated tryptophan and
phenylalanine metabolism. Adjuvant Bifidobacterium animalis subsp. lactis V9 (B.
lactis V9) restores gut microbial balance and increased quinaldic acid and catechol,
which enhance antitumor immunity and induce tumor cell death via caspase-dependent
apoptosis and ferroptosis. This benefit transfers via fecal supernatant, independent of
bacterial colonization. In a randomized, double-blind, placebo-controlled trial (n = 30),
adjunctive B. lactis V9 improves objective response rate (47% vs. 33%), disease control
rate (87% vs. 67%), and prolongs progression-free survival in responders, establishing
a metabolite-mediated strategy to overcome treatment resistance in high-CYFRA 21-1
NSCLC.
","dates":{"publication":"2026-07-21","submission":"2025-11-08"},"accession":"MTBLS13300","cross_references":{}}