MetaboLights

application/xml

ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/m_MTBLS13394_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/m_MTBLS13394_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/s_MTBLS13394.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/a_MTBLS13394_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/a_MTBLS13394_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13394The corrected and filtered peaks were then used for metabolite identification by searching a laboratory-built database and integrating public databases (HMDB, https://hmdb.ca/ and KEGG, https://www.kegg.jp/). Finally, metabolites with an identification composite score above 0.5 and a QC sample CV value below 0.3 were extracted. Positive and negative mode results were merged (retaining the substance with the highest identification level and the smallest CV value), resulting in the file all_sample_data.xlsx.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseAll samples were for two LC/MS methods. One aliquot was analyzed using positive ion conditions and was eluted from T3 column (Waters ACQUITY Premier HSS T3 Column 1.8 μm, 2.1 mm all_fetch_status all_status eb_eye_copy_status eb_eye_entry_counts eb_eye_fetch_status eb_eye_metabolights_complete.xml eb_eye_metabolights_compounds.copy eb_eye_metabolights_compounds.xml eb_eye_metabolights_studies.copy e_fetch_status europe_PMC_metabolights_studies.copy europe_PMC_metabolights_studies.xml head.xml studies.copy study.xml tail.xml thomsonreuters_metabolights_studies.copy thomsonreuters_metabolights_studies.xml 100 mm) using 0.1 % formic acid in water as solvent A and 0.1 % formic acid in acetonitrile as solvent B in the following gradient: 5 to 20 % in 2 min, increased to 60 % in the following 3 mins, increased to 99 % in 1 min and held for 1.5 min, then come back to 5 % mobile phase B witnin 0.1 min, held for 2.4 min. The analytical conditions were as follows, column temperature, 40 °C; flow rate, 0.4 mL/min; injection volume, 4 μL; Another aliquot was using negative ion conditions and was the same as the elution gradient of positive mode.Uridine Attenuates Osteoclastogenesis and Ovariectomy Induced Bone Loss by Modulating the PI3K/Akt–FoxO ROS Signaling Axis.The First Affiliated Hospital of Anhui Medical UniversityAries Axecell culturemass spectrometry assayThe sample stored at -80 °C refrigerator was thawed on ice. A 500 μL solution (Methanol : Water = 4:1, V/V) containing internal standard was added into the cell sample and vortexed for 3 min. The sample was placed in liquid nitrogen for 5 min and on the dry ice for 5 min, and then thawed on ice and vortexed for 2 min. This freeze-thaw circle was repeated three times in total. The sample was centrifuged at 12000 rpm for 10 min (4 °C). A 300 μL of supernatant was collected and placed in -20 °C for 30 min. The sample was then centrifuged at 12000 rpm for 3 min (4 °C). A 200 μL aliquots of supernatant were transferred for LC-MS analysis.Mus musculusThe raw mass spectrometry data were converted to mzML format using ProteoWizard, and peak extraction, alignment, and retention time correction were performed using the XCMS program. Peaks with a missing rate >50% across sample groups were filtered, and missing values were filled using KNN imputation. Peak areas were corrected using the SVR method.Treatmenthttps://www.ebi.ac.uk/metabolights/MTBLS13394axearies77@gmail.comRAW264.7 murine macrophage cells were obtained from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). Cells were maintained in DMEM supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin, and cultured in a humidified incubator at 37 °C with 5% CO2.For osteoclast differentiation, RAW264.7 cells were seeded and stimulated with RANKL (50 ng/mL) for 3–5 days, with medium replaced every 2 days. Cells were harvested at indicated time points for metabolomics sample collection.Metabolomicsuridineuntargeted metabolitesosteoclast differentiationosteoporosisuridineuntargeted metabolitesosteoclast differentiationosteoporosisAll the methods alternated between full scan MS and data dependent MSn scans using dynamic ex- clusion. MS analyses were carried out using electrospray ionization in the positive ion mode and negative ion mode using full scan analysis over m/z 75-1000 at 35000 resolution. Additional MS settings are: ion spray voltage, 3.5 KV or 3.2 KV in positive or negative modes, respevtively; Sheath gas (Arb), 30; Aux gas, 5; Ion transfer tube temperature, 320 °C; Vaporizer temperature, 300 °C; Collision energy, 30,40,50 V; Signal Intensity Threshold, 1*e6 cps; Top N vs Top speed, 10; Exclusion duration, 3s.3-dimethylsulfoniopropionaldehydeUridine-5'-diphosphateSolanidineActaraSer-Thr2',3'-Cyclic UMPEctoineCytidine-5'-monophosphate2-Butyne-1,4-diolMethapyrileneAltretaminegamma-Glutamyl-beta-cyanoalanineL-HistidineL-Allothreonine9-Octadecenal3-Methylcrotonyl Glycine4-(Butylamino)benzoic acidAllyl methyl sulfideIminodiacetic acidAlmotriptan1-Octadecanoyl-sn-glycero-3-phospho-(1'-myo-inositol)Nipecotic acidLPC(18:1/0:0)Bentazone2,6-Dimethoxyphenol3-Methoxybenzyl alcoholTosyl-L-lysine chloromethyl ketoneL-Leucyl-L-alanineN-Ethylglycine2',6'-Dihydroxy-4'-methoxyacetophenone4-HydroxybenzaldehydePicolinamide3,6,9,12,15,18,21-Heptaoxatricosane-1,23-diolalpha-CamphoreneChimyl alcohol1-(1Z-octadecenyl)-sn-glycero-3-phosphocholineN-Oleoyl-L-SerineEstradiolAlpinineHordenine7-Amino-9alpha-methoxymitosaneArginineRicinineChloroxineN-benzoyl-4-methoxyanthranilic acidgamma-Butyrolactone(2Z)-2-aminobut-2-enoic acidN1-acetylspermineGlutathione1-Acetylisatin1,2-Dioctanoyl-sn-glycerol11-Carbonyl-beta-acetyl-boswellic-acidalpha-Ergocryptine4,4'-SulfonyldiphenolFexofenadineNicotinate mononucleotideHexadecylaminePyracarbolidCaffeic acidIsodesmosine3-[(E)-1-[4-[2-(methylamino)ethoxy]phenyl]-2-phenylbut-1-enyl]phenolAdenosine-5'-diphosphateProline betaineMG(18:1/0:0/0:0)N-Acetyl-S-(N-methylcarbamoyl)cysteineOleamide5-(Aminomethyl)-2-fluorobenzonitrileThiazolidine-4-carboxylic acidPantothenic acidColfosceril palmitateD-Pyroglutamic acid1-Stearoyl-2-arachidonoyl-sn-glycero-3-phosphocholineUrsocholic acidBW-245CTetraethylammoniumcis-1,2-Dihydro-3-ethylcatechol1,3-DiisopropylbenzeneBenzocaineArachidonoyl PAF C-16TG(8:0/8:0/8:0)Trp-Iledelta-HexanolactoneSulcotrioneSodium 4-aminosalicylateAcetoacetic acidCarnitine C18:2(3-Hexadecoxy-2-icosa-8,11,14-trienoyloxypropyl) 2-(trimethylazaniumyl)ethyl phosphatetrans-2-Octenoic acidPhthalic acid1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphoethanolamineMaleimideDimethylallyl diphosphateBenzyl alcohol4-Methoxybenzaldehyde4beta-HydroxycholesterolApomorphine2-(((R)-2,3-Dihydroxypropyl)phosphoryloxy)-N,N,N-trimethylethanaminium4-Oxoniocarbonylbenzoate¦Ä-Valerolactam3'-Adenylic acid2-PhenylglycineChenodeoxycholic acidOrnithine2-(Octylthio)ethanol3-MethyladenineLPC(20:3/0:0)Cysteinyl-ArginineCysteamine2-(14,15-Epoxyeicosatrienoyl) Glycerol4-Methylbenzaldehyde4-HydroxycyclophosphamideN-Acetyl-D-GalactosamineSambutoxinPhe-ThrIsocitric acidGln-AspGlucuronic AcidDL-3,4-Dihydroxyphenyl glycolAloesinAdenine sulfateAdenosineAdenosine monophosphateGlucosaminic acidAllopurinolL-prolinylglycine zwitterionIsoprocarbTerbufosUric acidCathinone2-Hydroxycinnamic acidAconitic acidAcitretinNeolinustatin2-Hydroxydecanedioic acidCyclohexanol, 4-methyl-3-[(2E)-2-[(1R,3aS,7aR)-octahydro-7a-methyl-1-[(1R,2E,4R)-1,4,5-trimethyl-2-hexen-1-yl]-4H-inden-4-ylidene]ethylidene]-, (1S,3E,4S)-ChlorphenesinNoladin etherBenzoic acid(-)-AllantoinMelatoninL-Phenylalanine1-Stearoyl-2-docosahexanoyl-sn-glycero-3-phosphocholineIsoproterenolAripiprazoleModafinil Acid(1E,6R,11R,13R,14S,15S,19E,21E,23S,27R)-27-hydroxy-23-[(1S)-1-hydroxyethyl]-9,15-dimethylspiro[4,12,17,24-tetraoxapentacyclo[21.3.1.113,16.06,11.06,15]octacosa-1,9,19,21-tetraene-14,2'-oxirane]-3,18-dionePhenformin1-(1Z-octadecenyl)-2-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine3-Dehydrosphinganine1-Nonadecanoyl-2-eicosanoyl-glycero-3-phosphateGlycidyl oleatePhytanic acidN-Acetyl-L-tyrosineCarnitine C3:0Glyceryl monostearate2-Methyl-D-serineTrp-gluTetrahydrocortisonePhenol, 2-((ethylsulfonyl)methyl)-, methylcarbamate1-(1Z-hexadecenyl)-2-hexadecanoyl-glycero-3-phosphocholineNitrofurazoneDinex2-AminoindanPhenylethylmalonamide1-Tetradecyl-2-hexadecanoyl-sn-glycero-3-phosphocholineCabergoline1-Oleoyl-2-myristoyl-sn-glycero-3-phosphocholinePhenylacetaldehydeS-Methylglutathione2,6-DimethylpyridineD-threonineUmbelliferoneGelseminGuanineFosetyl1-Benzyl-3-cetyl-2-methylimidazolium iodide1-Methylnicotinamide(2S,4S)-1-Acetoxy-16-heptadecene-2,4-diol1-Palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamineTropate2,5-FurandimethanolLPC(O-18:0/0:0)LPC(16:0/0:0)Isopropyl dodecylfluorophosphonate2-(1-methyl-1H-imidazol-5-yl)acetic acidLupenone2,4-Dimethyl-2-penteneFlunisolide2H-Benzimidazole-2-thione, 1,3-dihydro-4-methyl-cis-4-Octenedioic acid3-MethylcyclohexanethiolXanthosineN-AcetylarylamineCitric acidEthyl p-methoxycinnamateVergentanDL-Propargylglycine1-Hydroxymidazolam2-UndecanoneGyromitrinAcetylcholine chlorideN'-HydroxybenzenecarboximidamideQuinestrolN-(2-hydroxyethyl)icosanamide1-nitrosonaphthalene(2S,3E,6R)-3,7-Dimethyl-3-octene-1,2,6,7-tetrolTyr-PhePipecolic acidProtopanaxadiolDazometisovaleryl-Val-Val-Sta(3xi,4xi)-Ala-Sta(3xi,4xi)-OHMethyl cinnamate2,7-Dihydroxy-5-methyl-1-naphthoic acidMirtazapineS-Sulfo-L-CysteineCarnitine C5:0Stearic acidFlavin adenine dinucleotide2-Oxazolidinone6,10,14-Trimethylpentadecan-2-oneDL-Ornithine2,5,8,11-Tetraoxatridecan-13-ol[2,3-Dihydroxypropyl(2-hydroxyethyl)amino]phosphonic acid1,1-DichloroethaneDermorphinCarnitineBisphenol ABenzofuran1,2-Dimethylnaphthalene2-Methyl-1-pyrroline2-PhenylpropionateO-acetyl-L-serine(2R,3R)-3-Methylglutamyl-5-semialdehyde-N6-lysineCyclohexanolPE-NMe(18:1(9Z)/18:1(9Z))2'-Deoxyadenosine-5'-monophosphate[2-[(4E,7E,10E,13E,16E,19E)-docosa-4,7,10,13,16,19-hexaenoyl]oxy-3-hexadecoxypropyl] 2-(trimethylazaniumyl)ethyl phosphateDiphenidol5-Methoxyindoleacetate1,5-AnhydrosorbitolHexetidineDiclofenacTestosterone enanthateL-TryptophanamideN-Acetylcysteine1-Myristoyl-2-palmitoyl-sn-glycero-3-phosphocholine3',5'-Cyclic IMPFluorouracilChloroform5alpha-Androstane-3alpha,17beta-diolThymineCytidine 3'-monophosphate(alpha-D-mannosyl)7-beta-D-mannosyl-diacetylchitobiosyl-L-asparagine, isoform A (protein)DL-LeucineL-3-Aminodihydro-2(3H)-furanoneMesalazineSarcosineMaltol2-Hydroxy-4-(methylthio)butyric acidMG(16:0/0:0/0:0)Procaterol hydrochloride hemihydrateN-PalmitoylglycineDiafenthiuronTaurinePC(20:4(5Z,8Z,11Z,14Z)/20:4(5Z,8Z,11Z,14Z))Amastatin1-(1Z-octadecenyl)-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolamineTrans-ZeatinArachidic acid3-Buten-1-olMethyl benzoate3-Hydroxy-2,4-dioxopentyl phosphateN-Methylbenzylamine2-HydroxybenzaldehydeSer-AspPramipexoleLithocholic acidcytidine monophosphate N-acetylneuraminic acidMet-TrpEthenzamideL-Threonine2-AminophenolCyclopamineBenzo[a]pyreneNADP nicotinamide-adenine-dinucleotide phosphate(2Z,4Z)-2-hydroxyhexa-2,4-dienoic acidL-LeucineTrigonellinePyridoxalLPC(16:1/0:0)2,5-Diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidineN-NonanoylglycineN-Acetyl-D-glucosamineBeclamideTyramine O-sulfateDanazolLPC(20:2/0:0)2-PhenylethynesulfonamideDeoxyuridine-5'-diphosphate3,4-Dimethyl-1,2-cyclopentanedioneRibonic acidN6-Succinyl AdenosineGuanosine-5'-monophosphateUbiquinone-21-Stearoyl-2-arachidonyl-sn-glycero-3-phosphocholineAminoethoxyacetic acidPralidoximumTetramethylsilaneImiquimodGeranylgeraniolCalcitriolp-AnisidineEthyl alpha-linolenateS-adenosylmethionine1-Phenyl-1-propanoneGossypetin 8-glucuronide 3-sulfateQuinaldic acidL-methionine sulfoxide3,4-DihydroxystyreneSulforaphaneOctopamineCarnitine C6:1L-Tryptophan(2R)-2-[(2R,3R,4S)-3,4-bis(2-hydroxyethoxy)oxolan-2-yl]-2-hydroxyethyl dodecanoateSyringaldehydeLPC(15:0/0:0)Glucosamine 1-phosphateMethoxyphedrineDammarenediol-I2-Amino-1-phenylethanolGlycocyamineDopamine3-Hydroxylidocainegamma-TocopherolN-Acetyl-beta-alaninePyrazinamideCholesterol4-aminobenzoateEicosadienoic acidDibutyl sebacate2,3-Dihydroxypropyl hexanoatePalmitoyl SerinolAntimony trioxideL-ValineMyosmineUracilN-oleoylglycineRhein11-DehydrocorticosteroneVidarabineCrotonic acidCuminaldehyde8,9-Epoxyeicosatrienoic acid1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholineAlanylarginineSinapic acid2-Phospho-D-Glyceric Acid4-Oxo-L-prolineCreatineDL-HomocysteineInosinic acidDiaveridineNandrolone decanoate4-(2,6,6-Trimethylcyclohexa-1,3-dienyl)but-3-en-2-oneOxiglutatione3-Nitroacrylic acidCytosineRisperidoneN-NitrosomethylethylamineEthosuximideDihydrorhodamine 1231-(4-Methoxyphenyl)-4-methylpentan-3-olPaliperidone(4E)-2-Oxohexenoic acidLPC(0:0/22:5)Angiotensin I/II (5-8)ParaxanthineLPE(0:0/24:0)Dephospho-CoANicotineAdrenic acid5-Aminoimidazole7alpha,12alpha-Dihydroxycholest-4-en-3-onep-CoumaroylagmatineTrimethylolpropane4-Hydroxy-9-fluorenonen-IsopropylbenzenesulfonamideLPC(18:0/0:0)4-Thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid, 3-methyl-7-oxo-3-(1H-1,2,3-triazol-1-ylmethyl)-, 4,4-dioxide, (2S,3S,5R)-Cumyluron2-Methoxybenzaldehyde1-Cyano-2-hydroxy-3-buteneAcetylpseudotropine1-Phenylethanol1-[(4-amino-3-methylphenyl)methyl]-5-(2,2-diphenylacetyl)-6,7-dihydro-4H-imidazo[4,5-c]pyridine-6-carboxylic acidHeptenophosVaccinosidePhensuximideHarmalineL-Glutamic acidS-(5-Adenosy)-L-HomocysteineNADP+Tetradecanal17-Octadecynoic acidTetraethyl pyrophosphateOmeprazole1,6-di-O-phosphono-beta-D-fructofuranosegamma-Linolenic AcidLPC(O-18:1)Uridine-5'-diphospho-N-acetylgalactosamine disodium saltNorepinephrineLPE(16:1/0:0)N-(dodecanoyl)-sphing-4-enine-1-phosphocholinePC(O-16:0/22:6(4Z,7Z,10Z,13Z,16Z,19Z))DimethylglycineGlutamineAminoacetone(2S,3S,4S,5R,6S)-6-[4-[2-Amino-1-(1-hydroxycyclohexyl)ethyl]phenoxy]-3,4,5-trihydroxyoxane-2-carboxylic acid3-Methylbenzyl alcoholPalmitic acidBardoxolone4,5-Dihydrovomifoliol2-Methylbenzyl alcoholBicinebeta-Glycerophosphoric acid3-p-Coumaroylquinic acidVitamin EAlanyllactate2,4-Diaminobutyric acidCarnitine C16:2TetranorbiotinCarnitine C16:04-Oxo-4-(pyridin-3-yl)butanalLeu-Gly-ProVitamin K1,10-Phenanthroline1,2,3,4,5-Benzenepentol2-HexenoylcholineAmlexanox(S)-Methylmalonic acid semialdehydeIsopetasoside2-Octene(2S)-2-amino-3-(3,4-dihydroxypyridin-1-ium-1-yl)propanoic acid1-hexadecanoyl-2-(5Z,8Z,11Z,14Z-eicosatetraenoyl)-sn-glycero-3-phosphate3-Methylglutaric acid(E)-2-Butenyl-4-methyl-threonineFluoromidineLPC(0:0/20:3)GlucovanillinMG(0:0/18:2(9Z,12Z)/0:0)LPC(0:0/20:4)Thiethylperazine1-[(2-Chloro-1,3-thiazol-5-yl)methyl]-2-methyl-3-nitroguanidineDeisopropylhydroxyatrazineN-AcetylputrescineScopoloside IPC(14:0/18:3(9Z,12Z,15Z))Xanthine2,3-Dihydroxy-4-methoxybenzoic acid(3Z,6Z,9Z)-dodecatrienol7-Ethoxy-4-methylcoumarinPC(O-16:0/O-2:0)CholinePC(8:0/8:0)Asparagine3-O-alpha-mycarosylerythronolide B2,3-DihydrobenzofuranTrp-ProNicotinic acid5-Hydroxymethyluracil4-Methoxy-2-[3-(4-methoxyphenyl)oxiran-2-yl]-2,3-dihydropyran-6-oneDL-MethionineL-Isoleucine2-(((2-Ethylhexyl)oxy)carbonyl)benzoic acidL-alpha-Aminobutyric acidtrans, trans-3,5-Octadien-2-one(2-(1-Butoxyethoxy)ethyl)benzene4-Amino-3-hydroxybutyrateNovaluron9alpha,11alpha,15R-Trihydroxy-17-phenyl-18,19,20-trinor-prost-5Z-en-1-oic acid, isopropyl esterMercaptopurineIsobutyrylglycine7-Methylxanthineo-TyrosineN-Oleoyl-4-aminobutyric acidGlufosinateEupatorinAsn-TyrPalmitoylcarnitineCefoxitinSwertiamarinGlycyl-L-leucineColladoninAPGPR EnterostatinSolasodinePyrroleDiosgeninL-Aspartic acidIsonicotinamideTetradecylamine4'-MethylacetophenoneSarsasapogeninIsolithocholic acidMethyl oleateLPI(18:1)delta-TocopherolN-Cyclohexylformamide4-Hydroxyphenylpyruvic acid6-Methyl mercaptopurineDodecyl glucopyranosideEpsilon-caprolactam(2S,3S)-2-amino-3-phenylbutanoic acidPiperidine5alpha-Cholestan-3-oneMetaxaloneTilarginineSpermidic acidCimetidineAminopentolLithospermosidePhosphocholineTrp-Asp1-o-Hexadecyl-2-oleoyl-sn-glycero-3-phosphocholineXylitolacetoacetate1-Heptadecanoyl-sn-glycero-3-phosphocholineAlfentanil1-HydroxypyreneNorbuprenorphineGlycyl-L-phenylalanineIsoeugenyl acetateMethylmalonic AcidVanillinCer(d18:0/12:0)5-(1,1-Dimethylheptyl)-2-[5-hydroxy-2-(3-hydroxypropyl)cyclohexyl]phenolPropiomazineEnantio-PAF C-16LumazineMechlorethamineN,N-dihydroxy-L-isoleucine1,2-Distearoyl-sn-glycero-3-phosphocholineRiboflavin2-Aminoacetaldehyde2-Oxo-3-(5-oxofuran-2-ylidene)propanoic acidStearoylcarnitineGlu-LeuN-NitrosodipropylamineBW-A868C1,2-Dipentadecanoyl-sn-glycero-3-phosphocholine2-Methylbenzoic acid4-Hydroxy-5-(3,4,5-trihydroxyphenyl)pentanoic acid2-Methyl-1-methylthio-2-buteneReboxetineNevadensinLeu-TyrTernatin heptapeptide1-O-Hexadecyl-2-arachidonoyl-sn-glycero-3-phosphocholineNicotinic acid adenine dinucleotideFucoxanthin2-Acetyl-3-methylpyrazineArecolineL-Proline(+/-)-EriodictyolN-succinyl-L,L-2,6-diaminopimelatePhenolL-dihydroanticapsinUridine-5'-monophosphate1-beta-D-GalactosylsphingosineAmpicillinAminosalicylic AcidCerP(d18:1/12:0)3-Hydroxyanthranilic acid[2-hydroxy-3-[(9Z,12Z)-octadeca-9,12-dienoyl]oxypropyl] 2-(trimethylazaniumyl)ethyl phosphateDicyclohexylaminecis-4-Hydroxy-D-proline3-Ethylphenol4-Ethylphenoll-Alanyl-l-leucineStearoylethanolamideDicyclomineL-lysinium(1+)4-PropylphenolDemeton-S-methyl1-(D-3-Mercapto-2-methyl-1-oxopropyl)-L-prolineDL-SerineMelamineFluoxetineMiglitolN-AcetylcadaverineIfenprodilDihydrocapsaicinBetulinic acidTriethylenetetramineLPE(18:1/0:0)Ciprofloxacin4-Pyridoxic acidVitamin D33-Hydroxymugineic acidIsovaleric acidGuanosineN-Acetyl-L-methionineN,N-Dimethylaniline N-oxideFluvoxamine Acid(¡À)-2-Propylthiazolidine4-Methoxycinnamic acid4-(5-Hydroxy-2-methyl-2-azabicyclo[2.2.2]octan-5-yl)-3-methylbutanoic acidTetradecanoylcarnitineHypotaurocyamineEuscaphic acid25-HydroxycholesterolCerbertin1-O-Palmitoyl-2-O-acetyl-sn-glycero-3-phosphorylcholineLactaldehydeCucurbitinDimethylallyl phosphate(S)-Remoxipride2'-Adenylic acidOxalacetic acidDichlorvos2alpha,3beta,12alpha,21,24(R),25-Hexahydroxylanost-8-eneD-MannosamineRaloxifeneL-Homocysteic acidEicosanedioic acidN-(Carboxyaminomethyl)urea11-nitro-1-undeceneMakisterone AIndole-3-carbinolAngiotensin IVPhenethylamineIsoxanthopterinTriacetinN-[2-[[[[(2S,3R)-2-(Hexadecanoylamino)-3-hydroxy-4-octadecenyl]oxy]hydroxyphosphinyl]oxy]ethyl]trimethylaminium2-Amino-1,6-dimethylfuro[3,2-e]imidazo[4,5-b]pyridineLauroyl diethanolamide2,4-Dihydroxyacetophenone 5-sulfate(1R,2R,9R)-1-piperidin-2-yl-3,15-diazatetracyclo[7.7.1.02,7.010,15]heptadecaneObacunoneDiethanolamineCeruleninIsoniazid1,4-DimethylphenanthreneMethyl 3-[[2-[4-(1-adamantyl)phenoxy]acetyl]amino]-4-hydroxybenzoateGlutathione Reducedform1-Palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholineTomatidineAromadendrinAklomideMG(18:2/0:0/0:0)5,5-Dimethyl-1-pyrroline N-oxideL-Tyrosine1-Methylpyrrolidine-2-carboxylic acidMalaoxonHypotaurine4-Chlorobenzenesulfonimidic acid1-IsothiocyanatohexaneAuraptenePhenylalanylphenylalanineDehydronuciferine1-DeoxynojirimycinNaftidrofuryl[3-[2-(dimethylamino)ethoxy-hydroxyphosphoryl]oxy-2-hexadecanoyloxypropyl] (Z)-octadec-9-enoate3-AminopropionaldehydeSpermidineTriethylamine1-Stearoyl-2-Arachidonoyl PC-d8IsofraxidinMethyl 2,5-dihydroxycinnamateMenadiol diphosphateS-Phenyl-N-acetylcysteine1-Myristoyl-2-stearoyl-sn-glycero-3-phosphocholine1-Octadecanoyl-cyclophosphatidic acid3,8'-Biflaviolin3-AminobutyrateLopinavirMecillinamLPE(18:0/0:0)N,N-dimethylarginineThiotepaNaphthyl dipeptide1-O-Hexadecyl-2-acetyl-sn-glycerolD-AllothreonineMethyl aminolevulinate1,2-di-[(11Z)-eicosenoyl]-sn-glycero-3-phosphocholine3-(4-methoxyphenyl)oxirane-2-carboxylic AcidFluoranthenebeta-N-Methylamino-L-alanineN-(9Z-octadecenoyl)-sphing-4-enine-1-phosphocholine3-Mercapto-2-butanoneZearalenoneIsovaleraldehydeLysergamide3-PyridinemethanolTetraethylene glycolEthyl maltol3-Methoxyanthranilate[2-[(9Z,12Z)-octadeca-9,12-dienoyl]oxy-3-[(Z)-octadec-9-enoyl]oxypropyl] 2-(trimethylazaniumyl)ethyl phosphateAstemizoleLeucyl-leucyl-tyrosine3-MethylcyclopenteneOctadecanamideAmoxapineAcetophenoneAlprenolol2-Hydroxypyridine6-HydroxybuspironeCreatinine1-(1Z-octadecenyl)-2-(4Z,7Z,10Z,13Z,16Z,19Z-docosahexaenoyl)-sn-glycero-3-phosphocholineTyr-Lys11-Oxahexadecanolide1-Docosanoyl-sn-glycero-3-phosphocholineAcetyl-L-carnitineDiuronPyridoxineMethyl hexadecanoic acidGibberellin A791-O-Hexadecyl-sn-glycero-3-phosphocholineCarbazoleDibasic sodium phosphateOctahydro-2,5,5,8a-tetramethyl-7H-1-benzopyran-7-oneFludarabine phosphate2-Phenylacetamide2-Hydroxyimipramine2-Amino-4-oxopentanoateEthyl benzoateOglufanidePropyzamideFosfosalHexaethylene glycol4-HydroxystyreneN-ethylmaleimideApocarotenalprotoporphyrin IXPalmitic amideLPE(0:0/24:1(15Z))Prednisolone tebutate2-(1-methyl-1H-imidazol-4-yl)acetic acid(r)-Methylmalonate semialdehydeCinobufagin10,11-Dihydroxy-9-(hydroxymethyl)-2,2,6a,6b,9,12a-hexamethyl-1,3,4,5,6,6a,7,8,8a,10,11,12,13,14b-tetradecahydropicene-4a-carboxylic acid5-FluorouridineBetaineDihomo-gamma-linolenoylethanolamideLuminolL-AlanineNimesulideGlu-Tyr5-Dimethylamiloride1-(1Z-hexadecenyl)-sn-glycero-3-phosphocholine3-Formylsalicylic acidPterin1-Aminocyclopropane-1-carboxylic acidbehenatePC(18:0/18:2)EnrofloxacinCytidine1-HexacoseneN-Acetyl-L-aspartic acid4-(Aminomethyl)benzoic acidUridine-diphosphate-n-acetylgalactosaminePyridostigmineL-LysineDigitoxigenin 3-[glucosyl-(1->6)-glucosyl-(1->4)-2,6-dideoxyribohexoside]5-Fluoro-2-methoxypyridin-4-amineLetrozoleAzelaoyl PAF(2S,4S)-2-amino-5-(carbamoylamino)-4-hydroxypentanoic acid2-Palmitoyl-rac-glycerolDodecanoic acidMisoprostolGenipinPhytolaccasaponin BOctylaminePhenethyl rutinoside6-MethyltetrahydropterinSphinganine7-NitroindazoleN-Methylalanine2-Phenylpropanal2-Iminobutanoic acidN-Acetyl-DL-phenylalanine beta-naphthyl esterMetoprololN-octanoylsphingosine 1-phosphateD-Glucose 6-PhosphateN-Palmitoylsphingomyelin4-Hydroxyphenylglyoxylic acidNootkatone4-Methylbenzyl alcoholHydroxyzine25-Hydroxyvitamin D2Naftopidil dihydrochlorideOxybutynin2-CyanopyridineEthisteroneIsonicotinic acidGallopamilAllantoinAngiotensin III4-Chloroaniline5-Methoxy-L-TryptophanCinnarizineL-Homoserine(E,9E)-9-(chloromethylidene)-N-[(E)-3-methoxy-5-[(2S)-2-methyl-5-oxo-2H-pyrrol-1-yl]-5-oxopent-3-enyl]-6-methyltetradec-4-en-13-ynamide1,11-Undecanedicarboxylic acidp-SynephrineGlyc-ProN-(2-Hydroxyethyl)hexadec-9-enamideKynurenic acidBuprenorphine GlucuronideRhynchophyllineLPC(O-16:0/2:0)(Z)-1-(Methylthio)-5-phenyl-1-penten-3-yne1-Aminocyclohexanecarboxylic acid2,8-QuinolinediolProlyl-cysteinePhytolEsmololCiclopiroxMethionineBarbituric acidStyrene Oxidep-Coumaryl alcohol 4-O-glucosideDeisopropylatrazineBenzamidineMephentermine3-O-alpha-L-Arabinopyranosyl-L-arabinoseStyreneEthyl hexadecanoateGlycerophospho-N-Palmitoyl EthanolamineDopaquinone2-(3,4-Dihydroxyphenyl)-5,7-dihydroxy-6-[3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]chromen-4-one2,6-Dichloro-1,4-phenylenediamine3-Sulfopyruvic acidCelastrol5-alpha-Dihydrotestosterone glucuronide4-Hydroxyhexan-3-oneGeranyllinaloolDeferoxamineBenzenemethanamine, N-butyl-4-chloro-2-oxo-3-phenylpropanoate[2-hexadecanoyloxy-3-[hydroxy-[2-(methylamino)ethoxy]phosphoryl]oxypropyl] (Z)-octadec-9-enoate3-(4-Hydroxyphenyl)chroman-7-ol1,2-Dioleoyl-sn-glycero-3-phosphoethanolamineL-Norleucine4-Hydroxy-3-nitrosobenzamideN-EthylacetamidePentadecanoic acidS-Formylglutathione5,6-Dimethylbenzimidazole1-Palmitoyl-2-glutaryl-sn-glycero-3-phosphocholine2-Hydroxy-cis-hex-2,4-dienoateTentoxinNiacinamideTrans-3-hydroxy-L-prolineXylobiose1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholineNitrofurantoinSerotonin(2R,4S)-2,4-diaminopentanoic acid2-Arachidonoyl glycerol-d5LaurylsulfateMelizame2(5H)-Furanone5-Hydroxymethyl-2-furancarboxaldehydeHeptethylene glycolFlurandrenolideHexylamineTioproninPipericineL-prolinamideCarnitine C9:0N-Acetylserotonin1-hexadecanoyl-2-[(Z)-octadec-9-enoyl]-sn-glycerol 3-(2-trimethylaminoethylphosphonate)o-Xylene2,3-Diaminopropionic acidAcetyleugenolPhytosphingosineN-(Hydroxymethyl)acrylamide2-AcetylpyrazineEtiocholanolone glucuronideProlyltryptophanIsopentenyl pyrophosphatepsi-Pelletierine1-Oleoyl-2-acetyl-sn-glycerolArachidonoyl Serinol5-keto-D-gluconic acidErgocalciferolFumitremorgin C(2R)-2-Hydroxy-2-methylbutanenitrilealpha-Hydroxyisobutyric acid5'-Deoxy-5'-(Methylthio) AdenosineCoumarin2,3-Bis-O-(geranylgeranyl)-sn-glycero-1-phospho-L-serine7,8-diaminopelargonateSpermineMyristic acid2-PhenylethanolHypoxanthineTemozolomideCrotonic acid betaineDihydro-5-propyl-2(3H)-furanoneHippuric acid2-ArachidonoylglycerolN8-Acetylspermidine3-Aminopiperidine-2,6-dioneCytarabineMethyl stearate4-Bromophenylacetic acidPyridoxal hydrochloride5-MethyluridineGlyceryl arachidonate3-(11,12-Dihydroxy-15,19-dotriacontadienyl)-5-methyl-2(5H)-furanone, 9CIEtilamfetamineEthyl tetradecanoateD-SerineDihydro-2-methoxy-2-methyl-3(2H)-thiophenoneEnoxacinAdenineBis(2-ethylhexyl) phthalateDimapritPhenolsL-SerineInosineS-Methyl-L-methioninePurineL-rhamnuloseLumichromeBufexamac2-PyrrolidinoneDibutyl disulfideLeu-Ile3,4-Dimethylbenzoic acidLPE(16:0/0:0)Firefly luciferinMelibiosePirimicarb1-Stearoyl-2-linoleoyl-sn-glycero-3-phosphoethanolamineEllagic acid1,2-CyclohexanedioneMethyl 3-methyl-2-oxopentanoateGentian violet cationCaffeineN-hexanoylsphingosineMyristoylcarnitineIndoraminPyridine-3,4-dicarboxylic acidStigmatellin Y3-HydroxybenzaldehydexI-Dihydro-2-methyl-3(2H)-thiophenoneCucurbitacin I3-MethylmethcathinoneErythronolide B2-Aminoethyl dihydrogen phosphateSebacic acidDihydrouracilcotinine N-oxidebeta-Sitosterol1,1,6-Trimethyl-1,2-dihydronaphthaleneCys-Phe2-MonooleinMaslinic acid1-Linoleoyl-3-docosahexaenoyl-sn-glycerol2-Propanamine, 2-methyl-N-(phenylmethylene)-, N-oxide2,2,6,6-Tetramethyl-4-piperidinoneDimethyl phthalate4-Ethylbenzoic acidPicolinic acidDiisobutyl phthalate2-Phenoxyethanol(-)-AnabasineEthyl carbamatePyroglutamic acidDanthronCyclocreatineEcdysonePerphenazine8,9-DiHETrEStigmasta-4,22-dien-3-oneIsopalmitic acidShikimic AcidCoriolic acidPalmitoleoyl Ethanolamide2-OxoarginineNonaprenyl-4-hydroxybenzoate6-AminonicotinamideAndrosteronePhosphatidylinositol 4-phosphatePhenyl beta-D-glucopyranoside4-Azaniumyl-4,5-dihydro-1,2-oxazol-3-olate1-arachidonoyl-sn-glycero-3-phospho-1D-myo-inositolN-Acetyl-L-alanineAcetylcholineEthyl methanesulfonatealpha-Ribazole-5'-phosphate9-nitro-9E-octadecenoic acidDibutyl phthalate1-Palmitoyl-2-myristoyl-sn-glycero-3-phosphocholineGlucurolactoneFFA(18:4)3-Palmitoyl-sn-glycerolSpinorphinPantetheine1-(9Z-octadecenoyl)-sn-glycero-3-phospho-(1'-sn-glycerol)L-Dihydroorotic acidValerosidateD-HomocysteineN,N-dimethylsphingosineAzaperoneLPC(22:6/0:0)Pentostam (TN)PE(22:4(7Z,10Z,13Z,16Z)/P-18:1(11Z))Benzaldehyde20-HydroxyecdysoneProlyl-Histidine1-Oleoyl-2-palmitoyl-sn-glycero-3-phosphocholineMiltefosineHistamineMethylamineN-Acetylneuraminic Acid(SA)ClopidolPyruvic AcidPoloxamer-188N-Methyl-D-aspartic acidVERBENONELPC(14:0/0:0)Methionine sulfoxideGuanosine-5'-diphosphateAllantoic acid1,3-Dicyclohexylurea1,3,6,8-TetrahydroxynaphthaleneO-Acetyl-L-homoserinePC(O-16:0/O-1:0)2-Amino-2-methyl-1,3-propanediolPhosphonoacetaldehydePyrrolidineMG(18:0/0:0/0:0)Epinephrine4-Chloro-L-phenylalanine8-demethyl-8-(2,3-O-dimethyl-alpha-L-rhamnosyl)tetracenomycin CBergaptenImidazoleacetic acidCarnitine-2-methyl-C4ClozapineFFA(18:3)1-pentadecanoyl-2-(9Z-hexadecenoyl)-sn-glycero-3-phosphocholinePsoralen3b-Hydroxy-5-cholenoic acidIsatinNorvalineBiopterinLeu-Glycis-7-Hexadecenoic Acid methyl esterfalseUridine Attenuates Osteoclastogenesis and Ovariectomy Induced Bone Loss by Modulating the PI3K/Akt–FoxO ROS Signaling AxisOsteoporosis is a metabolic bone disorder characterized by aberrant osteoclast activation, leading to excessive bone resorption and microarchitectural deterioration. Although the interconnection between osteoclast differentiation and cellular energy metabolism has been increasingly appreciated, the contribution of pyrimidine metabolism to this process remains largely undefined. Here, integrative transcriptomic and metabolomic profiling delineated a distinct metabolic remodeling during osteoclastogenesis and identified uridine as a pivotal metabolite exhibiting a pronounced decline upon RANKL stimulation. Functional and mechanistic studies demonstrated that exogenous uridine supplementation effectively restrained osteoclast formation and bone resorptive activity, concomitant with the downregulation of NFATc1 and CTSK expression. In OVX mice, uridine administration ameliorated trabecular microarchitecture, reduced osteoclast burden, and mitigated bone loss. Mechanistically, uridine inhibited PI3K/Akt phosphorylation, facilitated FoxO nuclear translocation, and suppressed ROS accumulation, thereby preventing NFATc1 activation and nuclear import. Collectively, this study uncovers a previously uncharacterized metabolic–signaling coupling mechanism linking pyrimidine metabolism to osteoclastogenesis and establishes uridine as a potential metabolic modulator for the prevention and treatment of osteoporosis. 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