Subsequently, the features were filtered across samples, resulting in a qualitative library matching report (including sample name, MS1 and MS2 matches) and a visualized matrix for relative quantification based on peak areas.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["For positive ion mode, the mobile phase consisted of water with 0.1% formic acid (Solution A) and 100% methanol with 0.1% formic acid (Solution B). For negative ion mode, it consisted of water with 0.05% acetic acid (Solution C) and methanol with 0.05% acetic acid (Solution D). The chromatographic gradient is shown in the table below, with a flow rate of 0.3 mL/min, a column temperature of 40 °C, and an injection volume of 3 μL.
"],"publication":["Evaluation of the Efficacy and Mechanism of FMT in Treating Malnutrition Following Gastroduodenal Reconstruction Surgery."],"submitter_affiliation":["The Second Affiliated Hospital of Hainan Medical University"],"submitter_name":["shiju chen"],"organism_part":["urine"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["a) Thaw the sample and vortex for 30 seconds.
b) Transfer 500 µL of the sample into a 2 mL centrifuge tube, and centrifuge at 4°C, 13,000 rpm for 5 minutes.
c) Pass the supernatant through a 0.22 µm aqueous phase syringe filter. Collect 80 µL of the filtrate into a 1.5 mL centrifuge tube, add 20 µL of 300 µg/mL internal standard, and vortex for 30 seconds.
d) Take 80 µL of the supernatant for instrumental analysis.
"],"organism":["Homo sapiens"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS13415"],"author":["Shiju Chen. The Second Affiliated Hospital of Hainan Medical University. 937817657@qq.com."],"data_transformation_protocol":["Raw data were converted to the universal ABF format using the AnalysisBase File Converter. On the MS-DIAL software platform, preprocessing steps including peak identification, retention time correction, and automatic integration were performed. This generated a visualized matrix containing metabolite ID, retention time, m/z, metabolite name, ion mode, peak area, etc.
"],"study_factor":["Treatment"],"submitter_email":["937817657@qq.com"],"sample_collection_protocol":["Fecal microbiota transplantation was performed in patients with malnutrition following gastric bypass surgery (13 cases showed clinical response, and urine samples from 12 of these effective patients were analyzed for metabolite changes before and after treatment). Urine samples were collected from the patients at 10:00 AM and stored in a -80°C freezer.
"],"omics_type":["Metabolomics"],"study_design":["malnutrition","Metagenomics","Ultimate 3000","Q Exactive HF-X( Thermo Fisher Scientific, USA)","untargeted analysis","urine","untargeted metabolites","Homo sapiens","experimental blank","fecal microbiota transplantation"],"curator_keywords":["malnutrition","Metagenomics","Ultimate 3000","Q Exactive HF-X( Thermo Fisher Scientific, USA)","untargeted analysis","urine","untargeted metabolites","Homo sapiens","experimental blank","fecal microbiota transplantation"],"mass_spectrometry_protocol":["Scan Mode: Positive and negative ions were scanned separately.
Ion Source: ESI
Spray Voltage: 2.5 kV (Positive); 2.5 kV (Negative)
Capillary Temperature: 325 °C
Sheath Gas Flow Rate: 50 Arb
Nebulizer Temperature: 300 °C
"],"additional_accession":[]},"is_claimable":false,"name":"Evaluation of the Efficacy and Mechanism of FMT in Treating Malnutrition Following Gastroduodenal Reconstruction Surgery","description":"Objective: To investigate the clinical efficacy of FMT in treating malnutrition following gastroduodenal reconstruction surgery, alongside changes in the metagenome and metabolome before and after transplantation.
Methods: A total of 20 patients underwent FMT (10 cases each in the mid-gastrointestinal tract TET group and the colonic TET group). Clinical response (defined as a BMI increase > 2%) was assessed one month post-FMT, whilst simultaneously observing the short-term, consecutive, fixed-time-point in situ (duodenal and ileocecal) microbial metagenome and urinary metabolome profiles.
","dates":{"publication":"2026-08-02","submission":"2025-11-28"},"accession":"MTBLS13415","cross_references":{}}