MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/m_MTBLS13465_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/m_MTBLS13465_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/a_MTBLS13465_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/a_MTBLS13465_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465/s_MTBLS13465.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13465<p>Raw data were collected and processed on Thermo Xcalibur Qual 4.0.27.19 (Thermo Fisher Scientific) software. The MS peaks were extracted at a mass tolerance of 5 ppm. For compound quantification, the corresponding peak area for each sample was used. Absolute concentrations were determined using calibration curves prepared from the corresponding standards (0.1 - 10 μg/ml). </p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Samples were separated on a Gemini C18 3 μm NX LC column 100*2.1 mm (Phenomenex) at 200 ul/min flow rate. Separation was done by a linear gradient of 90% acetonitrile in water, 10 mM ammonium formate, 0.1% formic acid (Buffer B) in 99.9% H2O, 10 mM ammonium formate, 0.1% formic acid (Buffer A): 1% B at 0 min, 50% B at 3 min, 99% B at 8 min, followed by 3 min wash at 99% B and 2 min equilibration at 1% B before the next run. UV data was collected at 220 nm.</p>Non-invasive imaging of defense responses in plants.Olga BelozerovaShemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of SciencesblankStandardleafmass spectrometry<p>Fresh leaves were homogenised in liquid nitrogen and lyophilised. Extracts were obtained from 25 mg of dry plant biomass using 1 mL of 70% methanol, filtered through 0.45 μm GF/PVDF (Phenex) filter and lyophilised in miVac machine. Dry residues were reconstituted in 100 µL of 70% methanol by vortexing, and transferred for LC-MS analysis.</p>Nicotiana benthamianablankStandardhttps://www.ebi.ac.uk/metabolights/MTBLS13465Olga Belozerova. o.belozyorova@gmail.com.Karen Sarkisyan. Imperial College London. karen@light.bio.<p>Raw data were collected and processed on Thermo Xcalibur Qual 4.0.27.19 (Thermo Fisher Scientific) software. The MS peaks were extracted at a mass tolerance of 5 ppm. For compound quantification, the corresponding peak area for each sample was used. Absolute concentrations were determined using calibration curves prepared from the corresponding standards (0.1 - 10 μg/ml). Data shown are means of three biological replicates.</p>Treatmento.belozyorova@gmail.com<p>Fresh leaves were homogenised in liquid nitrogen and lyophilised. Extracts were obtained from 25 mg of dry plant biomass using 1 mL of 70% methanol, filtered through 0.45 μm GF/PVDF (Phenex) filter and lyophilised in miVac machine. Dry residues were reconstituted in 100 µL of 70% methanol by vortexing, and transferred for LC-MS analysis.</p>Metabolomicsplant hormonePlantstargeted metabolitesplant hormonePlantstargeted metabolites<p>LC-MS analysis was carried out on an Ultimate 3000 RSLCnano HPLC system connected to a QExactive Plus mass spectrometer (Thermo Fisher Scientific). Samples were separated on a Gemini C18 3 μm NX LC column 100*2.1 mm (Phenomenex) at 200 ul/min flow rate. Separation was done by a linear gradient of 90% acetonitrile in water, 10 mM ammonium formate, 0.1% formic acid (Buffer B) in 99.9% H2O, 10 mM ammonium formate, 0.1% formic acid (Buffer A): 1% B at 0 min, 50% B at 3 min, 99% B at 8 min, followed by 3 min wash at 99% B and 2 min equilibration at 1% B before the next run. UV data was collected at 220 nm. MS1 spectra were collected in Negative ion mode at 30K Orbitrap resolution with 100-1000 a.e.m mass range. MS2 spectra were collected at 15K resolution. Fragmentation was done by HCD with stepped CE of 25, 30 and 35%. </p>211.1334324.2174falsePhytohormone profiling in Nicotiana benthamiana leaves upon mechanical damage and whitefly infestation<p>Jasmonic and salicylic acids are the major hormones involved in plant response to pests and pathogens. Non-invasive imaging of these hormones at the tissue and whole-organism levels has remained challenging. Here, we engineer autoluminescent plants that report activity of salicylic and jasmonic acids with up to 53-fold contrast. Using consumer-grade cameras, we image reporter Arabidopsis thaliana and Nicotiana benthamiana plants throughout normal development, and in response to attacks of pests and pathogens.</p>2025-12-052025-12-05MTBLS13465MTBLC36332MTBLC16914MTBLC139300MTBLC81897CHEBI:36332CHEBI:16914CHEBI:139300CHEBI:81897