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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/m_MTBLS13496_LC-MS_positive_hilic_metabolite_profiling_v2_maf_1.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/m_MTBLS13496_LC-MS_positive_hilic_metabolite_profiling_v2_maf_2.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/a_MTBLS13496_LC-MS_negative_reverse-phase_metabolite_profiling-1.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/a_MTBLS13496_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/s_MTBLS13496.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13496The Peak View 1.2 was used to identify metabolites and Multi Quant 2.1 was used to quantity bile acid based on the m/z value and sample retention time.Multi Quant 2.1 ParametersParameters   negative ionm/z wide     ±0.0025Gaussian smooth width   2 pointsRT half window 10 secMin. peak width 3 pointsMin. peak height 100Noise percentage 5 %Baseline sub. Window     2.00 minPeak splitting     2 points MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseAfter the pretreatment of plasma and ileum samples was completed, the samples were analyzed using the Eksigent ekspert ultraLC 110, which is coupled with the AB SCIEX Triple TOF 5600. The chromatographic column used was the XBridge Peptide BEH C18 column (1.5μm, 2.1 x 100 mm; Waters Corp.). The column temperature was 40℃, the flow rate was 0.4 mL/min, and the mobile phase consisted of A: water + 0.1% formic acid + 10 mM acetic amine, B: 80% methanol + 20% acetonitrile + 0.1% formic acid. For bile acids :The elution gradient began at 35% B (v/v) for 0.5 min; linearly increased to 60% B over 2.5 min; then to 80% B over 7 min; then to 90% B over 6 min; before finally decreasing linearly to 35% B over 4.5 min and holding for 2.5 min for column re-equilibration.For ceramides: The mobile phase consisted of (A) water and (B) a 1:1 (v/v) mixture of acetonitrile and isopropanol, both containing 10 mM ammonium formate and 0.1% formic acid. A gradient elution was performed with the following profile: 35% B at 0 min, ramped to 80% B at 2 min, further to 100% B at 9 min, held at 100% B until 15 min, then decreased to 35% B at 16 min, and finally held at 35% B until 20 min.4-Hydroxyphenanthrene exacerbates obesity by altering gut microbiota and bile acid metabolism.chao yuantianjin medical universityblood plasmafecesendometabolomemass spectrometryFor the quantitative profiling of bile acids and cermaide, an aliquot of 50 µL of plasma was mixed with 300 µL of ice-cold methanol and 10 µL internal standards mixture . Tissues and feces (10 mg) were weighed, and to homogenated at with 100μL of water at 3000 rpm using homogenizer. 10 μL of internal standard mixtureand 300μL ice-cold methanol were added for extraction through homogenization. All the mixture was allowed to stand at 4 oC for 10 min and vortexed for 45 s, and then centrifuged at 16000× g for 10 min at 4°C. An aliquot of 200 µL of superntant was transferred to another tube and then vacuum-dried. 50 µL of methanol was added, and the sample was re-vortexed at 16000× g for 10 min. The supernatant was transferred to an autosampler vial for LC-MS analysis, and the injection volume was 5 µL. Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS13496Chao Yuan. Tianjin Medical University. No. 22, Qixiangtai Road, Heping District, Tianjin, PR, China. hellgel_yc@126.com.The Peak View 1.2 was used to identify metabolites and Multi Quant 2.1 was used to quantity bile acid based on the m/z value and sample retention time.Multi Quant 2.1 ParametersParameters   negative ionm/z wide     ±0.0025Gaussian smooth width   2 pointsRT half window 10 secMin. peak width 3 pointsMin. peak height 100 Noise percentage 5 %Baseline sub. Window     2.00 minPeak splitting     2 points Detection batchhellgel_yc@126.comIn the 4-OHPhe intervention study, 8-week-old mice were acclimatized for one week. Mice were randomized into two groups based on whether they were exposed to 4-OHPhe. All mice received a high-fat diet (HFD) (Research diets, Cat D12492). Mice in the 4-OHPhe-exposed group received 4-OHPhe (2.5 mg/kg) orally daily for 8 weeks, which was dissolved in corn oil. The control group received the same volume of corn oil intervention daily. The dose of the gavage reagent was adjusted according to the body weight of the mice.Plasma was collected to determine bile acid levels and ceramide levels. WAT, BAT, feces, cecum and ileum were quickly frozen and stored at -80 ℃.Metabolomicsultra-performance liquid chromatography-mass spectrometryMulti-omics studymicrobiometargeted metabolitesultra-performance liquid chromatography-mass spectrometryMulti-omics studymicrobiometargeted metabolitesFor bile acids:Parameters TOF MS(-) Product Ion(-)IDAIon Source Gas 1(GS1) 50 50Ion Source Gas 2(GS2) 50 50Curtain Gas(CUR) 30 30Temperature(TEM) 550 550IonSpray Voltage Floating(ISVF) -4500 -4500TOF Masses(Da) 200-800 50-800Declustering Potential(DP) -80 -80Collision Energy(CE) -10 -45Collision Energy Spread(CES) ---- 20Ion Release Delay(IRD) ---- 67Ion Release Width(IRW) ---- 25For cermaide:Parameters      TOF MS(-)  Cal Product Ion(-)IDA    Cal(996.822)Accumulation time 0.249992     0.499992     0.099966     0.500007Ion Source Gas 1(GS1) 50  50  50  50Ion Source Gas 2(GS2) 50  50  50  50Curtain Gas(CUR) 30  30  30  30Temperature(TEM)      550 550 550 550IonSpray Voltage Floating(ISVF)    -4500    -4500    -4500    -4500TOF Masses(Da)   100-1200     100-1200     50-1200 50-1200Declustering Potential(DP) -80 -100      -80 -120Collision Energy(CE)  -10 -25 -35 -30Collision Energy Spread(CES)  ---- ---- 15  15Ion Release Delay(IRD)     ---- ---- 67  67Ion Release Width(IRW)    ---- ---- 25  25taurodeoxycholic acidC22:0 ceramidetauro-β-muricholic acidcholic acidtaurocholic acidC16:0 ceramidetauroursodeoxycholic acidursodeoxycholic acidglycochenodeoxycholic acidα-muricholic acidtaurohyodeoxycholic acidglycoursodeoxycholic acidC20:0 ceramideC18:1 ceramideglycocholic aciddehydrocholic acidlithocholic acidtauro-α-muricholic acidC24:0 ceramidetaurolithocholic acidC18:0 ceramidetaurochenodeoxycholic acidC24:1 ceramideglycodeoxycholic acidglycolithocholic acidhyodeoxycholic acidβ-muricholic acidchenodeoxycholic acidfalse4-Hydroxyphenanthrene exacerbates obesity by altering gut microbiota and bile acid metabolism Elevated exposure to 4-hydroxyphenanthrenes (4-OHPhe) is positively associated with obesity risk. However, the specific pathogenic mechanism remains unclear. Here, we used SHapley Additive exPlanations (SHAP) and logistic regression analysis to identify a strong association between 4-OHPhe and obesity. Mechanistically, exposure to 4-OHPhe significantly downregulates the expression of hydroxylamine reductase (hcp) genes in Bacteroides uniformis (B. uniformis), thereby inhibiting the removal of reactive oxygen species (ROS) and reducing the abundance of B. uniformis. The decline in B. uniformis abundance reduces hyodeoxycholic acid (HDCA) production and consequent activation of the intestinal farnesoid X receptor (FXR)-ceramide pathway, which ultimately results in the downregulation of white adipose tissue (WAT) thermogenic genes and obesity. Both B. uniformis colonization and HDCA treatment were found to promote this thermogenic effect. These findings demonstrate that 4-OHPhe exacerbates obesity via impairment of gut microbiota composition, thereby establishing a mechanistic link between environmental toxins and host metabolism.2025-12-212025-12-11MTBLS13496MTBLC81298MTBLC16359MTBLC31459MTBLC81243MTBLC9907MTBLC139136MTBLC27471MTBLC36259MTBLC28865MTBLC17687MTBLC16325MTBLC133057MTBLC9410MTBLC16525MTBLC80774MTBLC139138MTBLC36274MTBLC37998MTBLC16755MTBLC137822MTBLC89929MTBLC76751MTBLC76754MTBLC157596CHEBI:81298CHEBI:16359CHEBI:31459CHEBI:81243CHEBI:9907CHEBI:139136CHEBI:27471CHEBI:36259CHEBI:28865CHEBI:17687CHEBI:16325CHEBI:133057CHEBI:9410CHEBI:16525CHEBI:80774CHEBI:139138CHEBI:36274CHEBI:37998CHEBI:16755CHEBI:137822CHEBI:89929CHEBI:76751CHEBI:76754CHEBI:157596