For peak picking, the following parameters were used: centWave m/z= 25 ppm, peakwidth = c (10, 60), prefilter = c (10, 100). For peak grouping, bw= 5, mzwid =l0.025, minfrac= 0.5 were used. In the extracted ion features, only the variables having more than 50% of the nonzero measurement values in at least one group were kept. Compound identification of metabolites by MS/MS spectra with an in-house database established with available authentic standards.
"],"disease":[""],"extraction_protocol":["Samples were extracted with 400 μL of 10% formic acid in methanol-water (1:1, v/v), vortex for 30 s and then centrifuged at 2000 g and 4℃ for 5 min. Take an appropriate amount of supernatant and add 10% formic acid in methanol-water (1:1, v/v) to dilute 20 times and vortex for 30 s. Take 100 μL of supernatant and add 100 μL Trp-d3(20 ng/mL), vortex for 30 s. The supernatant was filtered through 0.22 μm membrane, and the filtrate was added to the LC-MS bottle.
"],"organism":["Homo sapiens"],"data_transformation_protocol":["The raw MS data (wiff.scan files) were converted to MzXML files using ProteoWizard MSConvert before importing into freely available XCMS software.
"],"study_factor":["Group"],"submitter_email":["ghc1399@163.com"],"metabolights_link":["https://www.ebi.ac.uk/metabolights/MTBLS13561"],"sample_collection_protocol":["All samples were blood specimens from female breast cancer patients. 'Normal' represents blood samples from patients who had not received cyclophosphamide chemotherapy, while 'CTX' denotes blood samples collected after a single course of cyclophosphamide chemotherapy. All blood samples were stored in a -80°C freezer.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"omics_type":["Metabolomics"],"instrument_platform":["Diode array detection MS - alternating - reverse-phase"],"study_design":["Ovary","Serine","Targeted Metabolomics"],"chromatography_protocol":["The samples were injected into the ACQUITY UPLC® BEH C18 Column (2.1×100 mm, 1.7 μm, Waters, USA), the injection volume was 5 μL, the column temperature was 40℃, and the mobile phase A-50 % methanol in water (containing 0.1% formic acid), B-10% methanol water (containing 0.1% formic acid). The gradient elution conditions were 0-6.5 min, 90-70% B; 6.5-7 min, 70-0% B; 7-14 min, 0% B; 14-14.5 min, 0-90% B; 14.5-17.5 min, 90% B. Flow rate 0~8.0 min, 0.3 mL/min; 8.0~17.5 min, 0.4 mL/min.
"],"publication":["Targeted Metabolomics Study: The Association Between Ovarian Function and Amino Acid Level Changes in Breast Cancer Patients Before and After Chemotherapy."],"curator_keywords":["Ovary","Serine","Targeted Metabolomics"],"submitter_name":["Haocheng Gu"],"submitter_affiliation":["Nanchang university"],"additional_accession":[]},"is_claimable":false,"name":"Targeted Metabolomics Study: The Association Between Ovarian Function and Amino Acid Level Changes in Breast Cancer Patients Before and After Chemotherapy.","description":"Cyclophosphamide (CTX) is a primary medicine for curing breast cancer which often caused premature ovarian insufficiency (POI). Our recent publication reveals that CTX induced POI by promoting the expression of SLC1A4, a transporter of serine efflux, in ovarian granulosa cells (GCs). Here, we report that there is a closed connection between the reduction of serum serine and ovarian hypofunction in the breast cancer patients treated with CTX or women of childbearing age who are suffered from the staying-up-late. Additionally, we observe that dietary serine supplementation protects mice from CTX-induced POI without altering its anti-breast cancer. Furthermore, we demonstrate that the elevated serine promoted S1P synthesis, and in turn, inhibit the nuclear translocation of Nrf2 and consequent HO-1 expression, to suppress ferroptosis in GCs. Our study reveal that the chemotherapy-induced or idiopathic POI shared the same mechanisms, indicating that serine is a critical factor for maintaining ovarian function.
","dates":{"publication":"2025-12-23","submission":"2025-12-23"},"accession":"MTBLS13561","cross_references":{}}