MetaboLights

application/xml

ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/m_MTBLS13598_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/m_MTBLS13598_LC-MS_positive_hilic_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/m_MTBLS13598_GC-MS_positive__metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/s_MTBLS13598.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/a_MTBLS13598_LC-MS_negative_hilic_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/a_MTBLS13598_LC-MS_positive_hilic_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598/a_MTBLS13598_GC-MS_positive__metabolite_profiling.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13598The metabolite data were log2- transformed (log2) to improve normality and mean centering before OPLS-DA analysis by SIMCA-16.0 software (Umetricus AB, Sweden). The variable importance in the projection (VIP) ≥ 1 in the OPLS-DA model and the absolute Log2FC (fold change) ≥ 1 were set for screening differential metabolites. Those having a p-value of t-test 0.05 and VIP ≥1 were deemed to be different between the two groups. Venn diagrams were employed to illustrate the number of divergent metabolites. An enrichment analysis of differential metabolites was conducted using the KEGG database (https://www.kegg.jp/kegg/pathway.html). KEGG enrichment analysis was performed using the Metware Cloud, a free online platform for data analysis (https://cloud.metware.cn).MetaboLightsPublicLiquid Chromatography MS - positive - hilicGas Chromatography MS - positiveLiquid Chromatography MS - negative - hilicGC-TOF-MS analysis was performed using an Agilent 7890 gas chromatograph coupled with a time-of-flight mass spectrometer. The system utilized a DB-5MS capillary column. 1 μL aliquot of sample was injected in split (10:1). Helium was used as the carrier gas, the front inlet purge flow was 3 mL min−1, and the gas flow rate through the column was 1 mL min−1. The initial temperature was kept at 50 °C for 1 min, then raised to 310 °C at a rate of 10 °C min−1, then kept for 8 min at 310 °C. The injection, transfer line, and ion source temperatures were 280, 280and 250 °C, respectively. The energy was -70 eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 50-500 at a rate of 12.5 spectra per second after a solvent delay of 6.25 min.Influence of Culture Conditions on Metabolite Diversity in Epichloë sinensis Strains.Lanzhou UniversityJunying LiuFestuca sinensismass spectrometryWeigh 500 mg sample into a 2 mL EP tube and add steel ball. 1000 μL extract (methanol: acetonitrile: water = 2:2:1) containing internal ribonucleol was added. 35 Hz grinding treatment for 4 min, ultrasonic in ice water bath for 5 min (repeat the step 3 times). Refrigerate at -40℃ for one hour . Centrifuge the sample at 4℃ at 12000 rpm for 15 min. Carefully remove 900 μL supernatant into 2 mL EP tube and blow dry. Add 90 μL methanol: acetonitrile: water = 2:2:1 for resolution. Centrifuge the sample at 4℃ at 12000 rpm for 15 min. Carefully remove 50 μL supernatant from each sample, take 20 μL from mixed QC. Dry extract in a vacuum concentrator.After evaporation in a vacuum concentrator, 80 μL of Methoxyamination hydrochloride (20mg/mL in pyridine) was added and then incubated at 80 ℃ for 30 min, then derivatized by 100 μL of BSTFA regent (1% TMCS, v/v) at 40 ℃ for 1.5 h. Gradually cooling samples to room temperature, 8 μL of FAMEs (in chloroform) was added to QC sample. All samples were then analyzed by gas chromatograph coupled with a time-of-flight mass spectrometer (GC-TOF-MS).Epichloe sinensishttps://www.ebi.ac.uk/metabolights/MTBLS13598Junying Liu. State Key Laboratory of Herbage Improvement and Grassland Argo-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. Jyliu2021@lzu.edu.cn.Wenbo Xu. State Key Laboratory of Herbage Improvement and Grassland Argo-ecosystems, Key Laboratory of Grassland Livestock Industry Innovation, Ministry of Agriculture and Rural Affairs; Engineering Research Center of Grassland Industry, Ministry of Education; College of Pastoral Agriculture Science and Technology, Lanzhou University, Lanzhou 730020, China. xuwb16@lzu.edu.cn.Pei Tian. Lanzhou University. tianp@lzu.edu.cn.Raw data analysis, including peak extraction, baseline adjustment, deconvolution, alignment and integration, was finished with Chroma TOF (V 4.3x, LECO) software and LECO-Fiehn Rtx5 database was used for metabolite identification by matching the mass spectrum and retention index. Finally, the peaks detected in less than half of QC samples or RSD>30% in QC samples was removed.Groupjyliu2021@lzu.edu.cnThree E. sinensis strains, 1, 2 and 84C, were isolated from F. sinensis seeds collected from Xiahe, Gansu Province (102°31'30'N, 35°12'0'E, strain 1), Qinghai Yushu (97°10'15.4'N, 32°49'38'E, strain 2), and Qinghai Ping'an (102°05.116N, 36°17.723E, strain 84C), respectively. The corresponding biological replicates for each group were 4 cases. The strains were stored at 4°C in the Microbial Germplasm Resource Bank of the Center for Grassland Microbiome of Lanzhou University.E. sinensis was grown on a sterilized PDA medium at 25℃ for four weeks to produce hypha. A 6-mm diameter puncher was then taken at the edge of the colony and inoculated into potato dextrose broth (PDB) and PDA media. Liquid culture was carried out using 250 mL conical flasks, containing 100 mL each of PDB medium. The three E. sinensis strains were inoculated and replicated 20 times per strain. The E. sinensis strains were shaken on an intelligent shaker at 25℃ and 145 rpm.Four samples were randomly collected from fungal endophytes mycelia cultured in PDB medium, PDB fermentation broth, and fungal endophytes mycelia cultured in PDA medium (Liu et al. 2004). The fungal endophytes hyphae and fermentation broth was collected as described by Luo and Tian et al. (2021). These samples were preserved on dry ice and subsequently transported to Biotree Biomedical Technology (Biotree Biomedical Technology Co., Ltd, Shanghai, China) for LC-MS and GC-MS analysis.MetabolomicsLiquid Chromatography/Tandem Mass SpectrometryGas Chromatography/Tandem Mass SpectrometryHyphaeIn VitroPDAPDBLiquid Chromatography/Tandem Mass SpectrometryGas Chromatography/Tandem Mass SpectrometryHyphaeIn VitroPDAPDBGC-TOF-MS analysis was performed using an Agilent 7890 gas chromatograph coupled with a time-of-flight mass spectrometer. The system utilized a DB-5MS capillary column. 1 μL aliquot of sample was injected in split (10:1). Helium was used as the carrier gas, the front inlet purge flow was 3 mL min−1, and the gas flow rate through the column was 1 mL min−1. The initial temperature was kept at 50 °C for 1 min, then raised to 310 °C at a rate of 10 °C min−1, then kept for 8 min at 310 °C. The injection, transfer line, and ion source temperatures were 280, 280and 250 °C, respectively. The energy was -70 eV in electron impact mode. The mass spectrometry data were acquired in full-scan mode with the m/z range of 50-500 at a rate of 12.5 spectra per second after a solvent delay of 6.25 min.di-2-furanylmethaneparaquat dichlorideaspartyl-isoleucinetyrosyl-asparaginevalyl-methioninearnamioln5-(4-methoxybenzyl)glutamineagnusidetg(18:2(9z,12z)/18:2(9z,12z)/18:2(9z,12z))2,3-diethylpyrazinen-ethylacetamide4-deoxyerythronic acidmillefinn-acryloylglycinehistidylproline diketopiperazinen-carboxyacetyl-d-phenylalaninevalyl-histidinebeta-d-galactopyranosyl-(1->4)-2-amino-2-deoxy-beta-d-glucopyranosyl-(1->6)-d-mannosecalystegine b2homoarecolinen-carbamoylsarcosineindole-3-propionic acidmethyldopamenthone 1,2-glyceryl ketaloxidized-adrenal-ferredoxinn4-acetylaminobutanal2,5-dimethylpyrazinede-o-methylsimmondsin1h-indole-2,3-dionecalystegine a6cis-5-caffeoylquinic acidmuramic acidtaxiphyllin4-oxo-2-nonenall-agaridoxinlysopc(18:2(9z,12z))pe(p-18:1(9z)/20:4(5z,8z,11z,14z))homo-l-arginined-aspartic acidtyrosyl-glycineethenyl acetatebeta-d-glucosamine1,3,5-trihydroxybenzene5-acetamidovalerate3,3,5-triiodo-l-thyronine-beta-d-glucuronosidebeta-aminopropionitrilelysope(18:1(9z)/0:0)3-acetamidobutanaldimethylbenzimidazoleserylalanine1-methyl-1,3-cyclohexadieneornithinecreatinine(2s,4s)-pinnatanine5,6-dihydroxyindoleguaiacol4-deoxyphysalolactonecotinine n-oxidebetaine aldehydeuralenneosidepc(20:3(8z,11z,14z)/14:0)serylthreonine6-methyladenineprolylglycine1-isothiocyanato-7-(methylthio)heptaneoleamideoccidentosiden-succinyl-2-amino-6-ketopimelate(e)-5-(3,4,5,6-tetrahydro-3-pyridylidenemethyl)-2-furanmethanol2,5-dihydro-2,4,5-trimethyloxazole5-methyl-2(3h)-furanone3'-o-methyl-(-)-epicatechin 7-o-glucuronidelysopc(18:1(9z))2-[(methylthio)methyl]-2-butenaln-(carbomethoxyacetyl)-4-s-chlorotryptophan2-o-methylcytosine2-hydroxypyridinegamma-l-glutamyl-l-pipecolic acid2-methoxy-5-methylpyrazine1-isothiocyanato-6-(methylthio)hexaneaflatoxin gm14-(3,4-dihydroxyphenyl)-2-hydroxy-1h-phenalen-1-oneaflatoxin gm2lauroyl diethanolamide4-acetamido-2-amino-6-nitrotoluene4-o-alpha-d-galactopyranosylcalystegine b2citalopram propionic acid3'-methoxyfukiic acidribitolmalondialdehyde1,3-dihydro-(2h)-indol-2-oned-pipecolic acid3-methyl-5-pentyl-2-furanundecanoic acidl-targinine5,9:6,9-diepoxy-4-megastigmene(2r)-2-hydroxy-2-methylbutanenitrile6-hydroxy-1h-indole-3-acetamide5-acetyl-2,4-dimethyloxazolephenylalanyl-threoninecellobiose3-furanmethanol glucosidehistidinyl-valine4',8-dimethylgossypetin 3-glucosideeicosapentaenoic acid2,4-dihydroxy-7,8-dimethoxy-2h-1,4-benzoxazin-3(4h)-one 2-glucosideisosojagollysopc(20:4(5z,8z,11z,14z))l-lysopinemalonylcarnitinel-lysine3-pyridylacetic aciddeoxyribosed-alaninebeta-guanidinopropionic acid1,2,3,4-tetrahydro-b-carboline-1,3-dicarboxylic acid3alpha,4,5,7alpha-tetrahydro-5-hydroxy-1h-isoindole-1,3(2h)-dionee-3179cadabicinecalystegine c1leucyl-valinethialdinediaminopimelic acidavenic acid bmannose 6-phosphatel-furosineprolyl-aspartatecalystegine b5lysyl-proline2-benzofurancarboxaldehyde2-propionylpyrrolelysope(16:0/0:0)acetylglycineboviquinone 4linalool 3,7-oxide beta-primeverosidephenylalanyl-gamma-glutamateniazirinn(omega)-hydroxyarginine5-(3-pyridyl)-2-hydroxytetrahydrofuranhydroxykynureninedemethylated antipyrinedeoxypyridinolinelimonexic acidgrossamidel-glutamine3-hydroxykynurenamineindicancnidimol 7-glucosidepersicogenin 3'-glucoside2-hydroxybenzaldehydehydroxyprolyl-hydroxyproline(z)-narceine imidehirsutin9-(beta-d-ribofuranosyl)zeatinn-acetyl-2,3-dihydro-1h-pyrrolebetainemethyl 3,4,6-tri-o-galloyl-beta-d-glucopyranosidephenethylamine glucuronidephenylalanyl-isoleucinealanyl-valineaminoadipic acidbeta-solamarinehippuric acidcer(d18:0/16:0)stachyoseacetylhydrazinen2-(3-hydroxysuccinoyl)arginine(2-methoxyethoxy)propanoic acidaspartyl-alaninenad(r)-pantothenic acid 4'-o-b-d-glucosideleucyl-tyrosinehypoxanthinecasimiroincinncassiol d2 glucoside1-methylhistidineallysinepentanenitrilethujyl 19-trachylobanoatepc(18:3(6z,9z,12z)/18:0)n-acetyl-l-glutamate 5-semialdehydebenzofurandieporeticenin7-methylinosinechorismateniacinamidepyridine2-methylbutyrylglycinecellulose triacetate2-(2-furanyl)-3-piperidinoltyrosyl-isoleucinevalyl-argininegerberinol5-pentyloxazole4,6-dihydroxy-2-quinolinecarboxylic acidn8-acetylspermidinetyrosyl-tyrosines-(3-methylbutanoyl)-dihydrolipoamide-enadpd-glyceric acidmethyl n-acetylanthranilate3-o-methylniveusin a1,3-diisopropylbenzenen-methylnicotinium20-hydroxyeicosatetraenoic acid3-(2-furanylmethyl)-1h-pyrrole3-methyluridined-prolineglycerol 1-propanoate diacetatepalmitoylethanolamideisovalerylglycine1-methyladenosinephosphonoacetatetromethamine4alpha-hydroxymethyl-4beta-methyl-5alpha-cholesta-8,24-dien-3beta-ol5-[2h-pyrrol-4-(3h)-ylidenemethyl]-2-furanmethanolalpha-(methoxyimino)-n-methyl-2-[[[1-[3-(trifluoromethyl)phenyl]ethoxy]imino]methyl]benzeneacetamideisolinderanolidebeta-d-glucopyranosyl anthranilate5-nitro-2-propoxyanilinen-acetylleucinedihydroxycitracridone ilinoleoyl ethanolamidequindoxinphytosphingosined-1-piperideine-2-carboxylic acid3,4-dihydro-5-(5-methyl-2-furanyl)-2h-pyrrole2,5-dimethyl-3-(2-propenyl)pyrazineglycylprolylhydroxyprolinen-acetyl-l-tyrosine3-hydroxylidocaineaustalide j1-o-galloylglycerol2-hydroxybenzyl alcohol4-(3,4-dihydroxyphenyl)-2,3-dihydro-2,3-dihydroxy-1h-phenalen-1-one(r)-2-hydroxysterculic acidisobutyrylglycinetyrosine methylesterl-methionineaica-ribosidecarbofurancis-6-nitro-p-mentha-1(7),2-dienedihydroxyacetone phosphate acyl ester5-(2-methylpropyl)tetrahydro-2-oxo-3-furancarboxylic acid(2e,4e)-2,7-dimethyl-2,4-octadienedioic acidbenzamidemethionyl-alaninebeta-d-galactopyranosyl-(1->4)-beta-d-galactopyranosyl-(1->4)-d-galactose5-(2-furanyl)-3,4-dihydro-2h-pyrrolegamma-l-glutamyl-l-methionine sulfoxideoctadecylaminepyridoxaminepipecolic acidgoyaglycoside cfapy-adeninerosmarinic acidd-arabinose 5-phosphatel-2-aminoethyl seryl phosphate(1r*,3r*,3's*)-1,2,3,4-tetrahydro-1-(2-thio-3-pyrrolidinyl)-beta-carboline-3-carboxylic acidd-glutamic acid7,8-dihydro-3-methylpyrrolo[1,2-a]pyrimidin-2(6h)-onepicraquassioside a4-oxo-1-(3-pyridyl)-1-butanone2-acetyl-1-methylpyrrolehydroxyprolyl-prolinel-n-(1h-indol-3-ylacetyl)aspartic acid2-phenylglycinen-acetyl-a-neuraminic acidn-acetyl-5-aminosalicylic acidaminoacetonedtdp-4-acetamido-4,6-dideoxy-d-galactosephenylalanyl-glycine8-isobutanoylneosolaniolthreoninyl-isoleucine1-(1-pyrrolidinyl)-2-propanonen-trans-feruloyl-4-o-methyldopamine2-ethylpyrazine2-o-(6-phospho-alpha-mannosyl)-d-glycerate3alpha,4,7,7alpha-tetrahydro-1h-isoindole-1,3(2h)-dionesmyrindiolosiden'-hydroxymethylnorcotininepc(20:4(8z,11z,14z,17z)/14:0)phlorin2-phenylacetamide1-[(5-amino-5-carboxypentyl)amino]-1-deoxyfructosetyrosyl-alanine2-acetylfuranmaltopentaosep-hydroxyfelbamateglycylleucinemethyl 2-aminobenzoatedioxinoacrimarine a3-(4-hydroxyphenyl)-1-propanolaminofructose 6-phosphatemaltohexaosetenuazonic aciddimethyl fukiic acidacrylamideprulaurasinethylmalonic acidprolylhydroxyproline11-dehydro-thromboxane b24-oxo-4-(3-pyridyl)-butanamideleukoaminochromebutyrylcarnitine2-ethoxy-6-methylpyrazinehydroxyphenylacetylglycinehomocarnosineprotoanemoninquercetin 3-o-glucuronideglycyl-phenylalaninen-ethylglycine2'-deoxyguanosine 5'-monophosphate1-isothiocyanato-8-(methylthio)octaneanandamidel-nicotianineacetone cyanohydringamma-glutaminyl-4-hydroxybenzeneglucose 1-phosphatel-tryptophans-adenosylmethionined-fructosazinel-isoleucinezanthodiolineprolyl-alanine1-hydroxy-10-methylacridone2-acetyloxazolecreatinemethylimidazole acetaldehyden-acetyl-beta-alanineisotheaflavin3'-glucosyl-2',4',6'-trihydroxyacetophenonenivalenolfalcarindionecysteineglutathione disulfide7,8-dihydropteroic acidthreoninyl-alanineethyl 2-aminobenzoateudp-n-acetyl-alpha-d-galactosamineisopropylpyrazineaflatoxin g22,3-dihydro-6-methyl-1h-pyrrolizine-5-carboxaldehydeepicatechinalanyl-prolineblasticidin s2-furancarboxaldehyden-carboxyethyl-g-aminobutyric acid3-(5-methyl-2-furanyl)butanalo-acetylethanolaminediacetylxanthurenic acidtyramine glucuronide1h-indol-3-ylacetyl-myo-inositolmarmesin rhamnosideergosterol2,6-di-tert-butyl-4-methylphenol8alpha,13r-epoxy-14-labden-19-oic acidistanbulin bxanthine2,3-dihydro-6-methyl-5-(5-methyl-2-furanyl)-1h-pyrrolizinepc(20:2(11z,14z)/14:0)s-propyl-l-cysteine2-hydroxy-7-methoxy-2h-1,4-benzoxazin-3(4h)-onedopamineergothioneinequeuinel-alpha-amino-1h-pyrrole-1-hexanoic acidadenosine 2'-phosphate3-isopropylmalatel-dopanicotinamide ribotideglutaminylproline2-methylfuran4-trimethylammoniobutanalmethoxypyrazinelysyl-gamma-glutamateisoleucyl-lysinedg(14:0/22:6(4z,7z,10z,13z,16z,19z)/0:0)(e)-2-butenalnicotine2-o-galloylsucroseethylphosphaten-a-acetyl-l-arginineepinephrinevinylacetylglycinen-[4'-hydroxy-(e)-cinnamoyl]-l-aspartic acid3-methylhistidinetheaspirone aacetaminophendulciol dvalyl-threoninemethyl 2-furoate2,5-dimethyloxazoleporphobilinogencinnamyl benzoatequeuosinegentiatibetinen1-methyl-4-pyridone-3-carboxamide3-hydroxysebacic acid2-ethyl-4-methyloxazoleleucyl-glycinepyro-l-glutaminyl-l-glutaminelinoleamidel-trans-4-methyl-2-pyrrolidinecarboxylic acid2-methoxy-3,5-dimethylpyrimidinenorcapsaicindg(18:3(9z,12z,15z)/18:1(11z)/0:0)pc(18:1(11z)/14:0)6-acetyl-2,3-dihydro-2-(hydroxymethyl)-4(1h)-pyridinonequercetin2-amino-3-methylbenzoatealpha-hydroxy-1-methyl-1h-indole-3-propanoic acidphenylbutyrylglutaminenopalinic acidn-a-acetylcitrullinesphinganine1,4,5-naphthalenetriolbetanidinfalimint3-amino-2-piperidone2-methyl-1-hydroxybutyl-thpposmaronin(3beta,5alpha,6alpha,7alpha,22e,24r)-5,6-epoxyergosta-8,14,22-triene-3,7-diolpc(18:3(6z,9z,12z)/18:1(11z))alanyl-isoleucineprolyl-arginine1-methyladenine2-aminoacrylic acidpyridoxal3-hydroxyisovalerylcarnitines-methyl propanethioate(e)-2-propenyl [3-(2-propenylthio)-2-propenyl] sulfatesemilepidinoside asemilepidinoside bsedoheptulose 1-phosphategonyautoxin vlysopc(15:0)1,2-dihydro-1,1,6-trimethylnaphthalenecoffeastereneginkgotoxinophthalmic acidn-methyl-l-glutamic acidanigorufoneorotic acidfenoprofen glucuronidevalyl-gamma-glutamatedeoxyfructosazinel-threoninen-lactoyl ethanolamine2-(ethylamino)-4,5-dihydroxybenzamide12-oxo-2,3-dinor-10,15-phytodienoic acidn-succinyl-l,l-2,6-diaminopimelategalactose-beta-1,4-xylosel-kynureninen-acetyltryptophanphenobarbitalbutyl 3-o-caffeoylquinatephenols-acetyldihydrolipoamide-e2-oxoargininemollicellin a3-methyladenine3-isomangostin hydratedeoxyadenosinepiplartineleucyl-threoninepyridoxinedg(22:5(4z,7z,10z,13z,16z)/14:0/0:0)(+)-2,3-dihydro-3-methyl-1h-pyrroleciticolinen-hydroxy-l-tyrosine6,7-dihydro-2-methyl-5h-cyclopenta[b]pyrazine5-(3,4-methylenedioxyphenyl)pentanoic acidphenylacetaldehyde2-methyl-3-(2-methylpropyl)pyrazineepidermin2-methoxy-(3 or 5 or 6)-isopropylpyrazinepuddumin an-acetylglutamined-serinepyrrolidinethaumatin b, recombinant3-dehydrosphinganinenervonyl carnitinedimethylethanolaminen,n-dimethylformamidemaltol3'-o-methyladenosinestearidonic acidbenzyl o-[arabinofuranosyl-(1->6)-glucoside]galactinol5,6,7,8-tetrahydro-4-methylquinolinephenylacetylglycinealanyl-argininephenylalanyl-serine3'-ampsuccinyladenosine3-acetylpyridinealpha-[3-(nitrosoamino)propyl]-3-pyridinemethanolalanyl-tyrosine1-pyrroline-4-hydroxy-2-carboxylate3-hydroxy-carbofuran4,5-dimethyloxazoledihydroneopterin phosphateresolvin d53-dehydroxycarnitinetrimethyloxazolevalyl-prolines-(2-methylpropionyl)-dihydrolipoamide-efurfuryl acetate3-aminocaproic acid1-deoxynojirimycin1-pyrrolidinecarboxaldehyde5-(3',4',5'-trihydroxyphenyl)-gamma-valerolactonel-asparagine2-keto-6-acetamidocaproateenalaprilatxi-3,5-dimethyl-2(5h)-furanone7-hydroxymethyl-12-methylbenz[a]anthracene sulfated-xyluloseaspartyl-valine6-(alpha-d-glucosaminyl)-1d-myo-inositol3-dehydrocarnitine1h-indole-3-acetamidepseudoecgoninearginyl-gamma-glutamateimidazoleacetic acid ribotidel-carnitinepyrimidine1-(3-methyl-2-butenoyl)-6-apiosylglucoseo-acetylserinediethyl tartrate2-hydroxy-4,7-dimethoxy-2h-1,4-benzoxazin-3(4h)-onen-cyclopropyl-trans-2-cis-6-nonadienamidebenzyl cinnamateleucyl-isoleucineisoleucyl-threoninegamma-glutamylglutamic acid(5alpha,10alpha)-3,7(11)-eudesmadien-2-one2-amino-1,7,9-trimethylimidazo[4,5-g]quinoxalinen2-methylguanine3-(1-pyrrolidinyl)-2-butanonetriglochinin5'-methylthioadenosinelaccarinpi-methylimidazoleacetic acidl-tyrosinealdosine(r)-2-nitro-p-mentha-1,5-dienen-acetylneuraminic acidvalyl-lysinel-galactosetryptophyl-proline2-hydroxy-6-tridecylbenzoic acidhydroxycotinine1-pentanesulfenothioic acid1,3-dimethyluracilcyclotetradecanedinoseb acetate2,4-dimethyloxazolen2-(2-carboxymethyl-2-hydroxysuccinoyl)argininetetraphyllin b4-(methylthio)-2-butanolsec-butylaminetetramethylpyrazinemyricetinsolavetivonen6-methyladenosine2',4'-dihydroxy-6'-methoxyacetophenoneiprobenfos4-methylpyrrolo[1,2-a]pyrazineisopropyl apiosylglucosidecytidinenitrites-acetyldihydrolipoamideo-xylene4-aminohippuric acidl-4-hydroxyglutamate semialdehydequinonesedoheptulosehistaminemandelonitrile1-(hydroxymethyl)-5,5-dimethyl-2,4-imidazolidinedionehomoanserine3,4-dihydro-4-[(5-methyl-2-furanyl)methylene]-2h-pyrrole1-ethyl-1h-pyrrole-2-carboxaldehyde2-galloylglucosedihydrobiopterinsaxitoxindopamine quinoneglucosylisomaltolacetylhomoserinecynaroside a1,4-dimethoxyglucobrassicinpyrazinediethanolaminehydrocotarninen-methyl-d-aspartic acid5'-hydroxycotinine2'-o-methyladenosinetg(18:1(9z)/16:0/22:5(7z,10z,13z,16z,19z))5-aminolevulinic acidcarbendazimsuberylglycinedihydro-2,4-dimethyl-6-(1-methylpropyl)-4h-1,3,5-dithiazineaquifoliunine eiiiindole2,3-dihydro-5-methyl-1h-pyrrolizine-7-carboxaldehydetrimethylaminoacetone1-kestosealpha-methylstyrene5-oxo-2(5h)-isoxazolepropanenitrile2-isopropyl-3-methoxypyrazinevalyl-phenylalaninenicotinamide n-oxidethiamine2'-deoxymugineic acidprolyl-serinecalabaxanthonemelledonal clysope(18:2(9z,12z)/0:0)n-acetylornithine4-hydroxy-4-(3-pyridyl)-butanoic acidaspartamen6-carbamoyl-l-threonyladenosinemethyl (3x,4e,10r)-3,10-dihydroxy-4,11-dodecadiene-6,8-diynoate 10-glucosidegamma-glutamyltyrosinequinolinemethyl 6-o-galloyl-beta-d-glucopyranosidefagominebetamipron2-furanmethanol1,4-dideoxy-1,4-imino-d-ribitol2-methylbutylaminenicotinic acidalanyl-leucinethreoninyl-tyrosines-cysteinosuccinic acidglutaminyltyrosinehypoglycin bglycylprolinepyrrole-2-carboxylic acidhistidinyl-alaninekoeniginequinone aarecaidine2-aminonaphthalenethiomorpholine 3-carboxylatepiperidineparaldehyden2-fructopyranosylargininefalcarinolonen-phenylacetylaspartic acid3-ethyl-5-methyl-2-vinylpyrazinesulforidazine2-aminoheptanedioic acid3,4-dihydroxymandelic acidlacto-n-biose iaspergillomarasmine a2-(methylamino)benzoic acidtetrahydropentoxyline1-nitroheptaneprolyl-threonineleucyl-argininel-histidinepe(18:1(9z)/18:2(9z,12z))glycerol 1,3-di-(9z,12z-octadecadienoate) 2-(9z-octadecenoate)vanillylamineethyl 3-hydroxyoctanoate o-[glucosyl-(1->6)-glucoside]4-hydroxyphenylacetylglutamic acid3-hydroxymethylglutaric acidd-1-amino-2-pyrrolidinecarboxylic acid3-methoxytyrosine1-methyl-3-(2-thiazolyl)-1h-indoleadenosine 3',5'-diphosphatephenylacetylglutamine1,3,5-trihydroxy-10-methylacridone3-hydroxybenzoic acidtocopheronic acidsmall bacteriocin5-hydroxyisouraten-acetylvanilalaninelevan1-octen-3-yl primeveroside3-hydroxymugineic acidgamma-glutamylisoleucine5-methylcytidinenicotinic acid mononucleotide4'-hydroxyflurbiprofen2-aminomuconic acid semialdehydep-aminobenzoic acidsarmentosinkinetineudesmic acid4-[(hydroxymethyl)nitrosoamino]-1-(3-pyridinyl)-1-butanone4-o-alpha-d-galactopyranuronosyl-d-galacturonic acidp-cresol glucuronidehistidinyl-glutaminetriethylaminestyreneavocadene 2-acetateblumenol c o-[rhamnosyl-(1->6)-glucoside]ps(16:0/18:2(9z,12z))armillanecitalopram aldehyde1-aminocyclopropanecarboxylic acidvalyl-tyrosinemelaminegarcimangosone dmethyl nicotinate2,3,6,7-tetrahydrocyclopent[b]azepin-8(1h)-one(z)-1-(methylthio)-5-phenyl-1-penten-3-yneethyl isopropyl disulfide3-(methylthio)-1-propene2,5-dihydro-2,4-dimethyloxazole3-methylguaninebeta-carboline4-hydroxystachydrinecinnamaldehydetyrosyl-threoninedihydro-6-isopropyl-2,4-dimethyl-4h-1,3,5-dithiazinequercetagitrinphyscionincardoltrieneleucyl-tryptophanl-histidine trimethylbetainecamelininpterolactamimidazole acetol-phosphate4-aminophenolpc(22:6(4z,7z,10z,13z,16z,19z)/16:1(9z))abscisic alcohol 11-glucosidegentiotrioseprunasinasymmetric dimethylargininesquamolonen1-(2-hydroxyethyl)flurazepaml-threo-3-phenylserinetetrahydroharmol4-caffeoyl-1,5-quinolactone5-hydroxy-l-tryptophankanzonol kformononetin 7-o-glucuronidebenzaldehyde10-hydroxycarbazepineguaninemethyl 1-methoxy-1h-indole-3-carboxylatehelipyronel-cystathionine3-oxo-carbofuran(r)-dihydromaleimideepoxy-hexobarbitalinosine 2'-phosphateribothymidine3-amino-1,4-dimethyl-5h-pyrido[4,3-b]indoledihydroferuloylglycinedopamine glucuronidedesmethylclomipraminennal-n-oxide2-biphenyloln-acetylmuramate2,3-dihydro-5-(3-hydroxypropanoyl)-1h-pyrrolizineosmundalactoneglutaminylaspartic acid6-galloylglucosel-2-amino-3-oxobutanoic acid4-hydroxy-2,6-dimethylanilinedeoxyadenosine monophosphate2-methylbutyroylcarnitineethylbenzene1-pyrroline-2-carboxylic acidtriptohypol f(e)-2-methyl-2-buten-1-ol o-beta-d-glucopyranosidelysyl-valinep-octopamine6-methylquinoline2-(3,4-dihydroxybenzoyloxy)-4,6-dihydroxybenzoate1-pyrroline-5-carboxylic acid3-aminopropionaldehyde15-acetoxyscirpene-3,4-diol 4-o-a-d-glucopyranosided-maltose6-chloro-n-(1-methylethyl)-1,3,5-triazine-2,4-diaminegamma-glutamyl-se-methylselenocysteinegamma-glutamylvaline4-acetylimidazo[4,5-c]pyridine2-ethoxy-5-methylpyrazinepalmitic amideglycineamideribotideyuccaol cinositol cyclic phosphaten-carbamoyl-2-amino-2-(4-hydroxyphenyl)acetic acidercalcitriol1-amino-propan-2-oll-cis-3-amino-2-pyrrolidinecarboxylic acid2-isopropylmalic acid2-amino-3,4,8-trimethyl-3h-imidazo[4,5-f]quinoxaline8-methoxykynurenatecytokinin bdg(16:0/16:0/0:0)dl-glutamatepimethixene2,2,4,4,6,6-hexamethyl-1,3,5-trithiane4-amino-2-methylenebutanoic acidoxfendazolemytilin a(2s,4r,5s)-muscarinemammea a/ab cyclo f(r)-pelletierine4-hydroxy-5-phenyltetrahydro-1,3-oxazin-2-onen-methylcalystegine b2acetylvalerenolic acidvalyl-serinemethionine sulfoxideharmanineisoleucyl-phenylalanine3-ethyl-2-methoxypyrazined-galactosen4-acetylcytidine4-(4-methyl-3-pentenyl)-3-cyclohexene-1-carboxaldehydeguanidinosuccinic acid2-acetyl-3-ethylpyrazinen,n'-bis(gamma-glutamyl)cystinehumulinic acid aprostaglandin i2alpha-chaconine(2s,2's)-pyrosaccharopinen-acetylputrescine3-methylsuberic acid2-oxo-4-methylthiobutanoic acidprolyl-asparaginen-[(5-hydroxy-2-pyridinyl)methyl]adenosinedcmprosmic acid(-)-3-cyanomethyl-3-hydroxy-1h-indol-2(3h)-one2-amino-4-oxo-6-(1',2'-dioxoprolyl)-7,8-dihydroxypteridineglutaminylalaninealpha-[3-[(hydroxymethyl)nitrosoamino]propyl]-3-pyridinemethanolcohibin bcohibin ccalystegin a3cohibin a5-aminoimidazole ribonucleotide1-phosphatidyl-d-myo-inositoldistichonic acid azizybeoside i2,6-diaminohexanoic acidmusanolone dmusanolone cglycerophosphocholinemusanolone fmusanolone edeoxycytidine3-hydroxyadipic acid 3,6-lactonefructosaminel-acetylcarnitineformyl-5-hydroxykynurenamine1-methylguanosinephosphorylcholinehomocitrullineseryltyrosinededimethylchlorpromazine8-hete1-o-p-coumaroyl-(b-d-glucose 6-o-sulfate)leucyl-gamma-glutamate4-hydroxybenzaldehydearmillaridinagaritinalniazirinin(2e,11z)-5-[5-(methylthio)-4-penten-2-ynyl]-2-furanacroleindeacetyldiltiazem2-methyl-3-propylpyrazineprostaglandin e1prostaglandin e21-pyrrolinephosphoric acidpantetheine 4'-phosphatebis(2-methylpropanoyloxy)-9,10-epoxy-p-mentha-1,3,5-trienemontecristinn-acetyldopaminen(6)-(1,2-dicarboxyethyl)amp4-guanidinobutanoic acidkojibiose2,6-toluenediamines-4-hydroxymephenytoin2-(4-methyl-1,3-pentadienyl)anthraquinone8-hydroxyadeninel-prolinecyclic gmpglycerylphosphorylethanolamine(2r,3r,4r)-2-amino-4-hydroxy-3-methylpentanoic acidserylleucinediethylcarbamazine n-oxidel-glutamic acidtiglylglycinel-pyridosinebox b6,7-dihydro-5-methyl-5h-cyclopenta[b]pyrazinebox aolomoucinesterebin en-benzoylaspartic acidglutarate semialdehydeneotrehaloseloquatosiden2,n2-dimethylguanosinedihydrozeatin1-isopropyl-2,3-dimethylcyclopentaneglucosamine 6-phosphatethreoninyl-argininen-acetyl-b-glucosaminylaminen,n'-diacetylhydrazinetyrosyl-serineglutamylglutamic acidcotinine methonium ionpteroside zsecoeremopetasitolide bbetonicine6''-o-malonyldaidzin4-hydroxy-2-butenoic acid gamma-lactonel-acetopine4-trimethylammoniobutanoic acid1,3,7-trimethyluric acidmg(0:0/18:3(6z,9z,12z)/0:0)l,l-cyclo(leucylprolyl)l-homoserinecpa(18:2(9z,12z)/0:0)argininic acidacetylcholinelysopc(18:3(6z,9z,12z))acetoacetic acidsakacin pn-acetylserine(22e, 24x)-ergosta-4,6,8,22-tetraen-3-onechavicolbergaptentyrosyl-hydroxyprolineturanoseprolyl-gamma-glutamate2-acetyl-3,5-dimethylpyrazine2-ethoxy-3-methylpyrazinel-2-amino-5-hydroxypentanoic acidvalyl-valineglycyltyrosinen-methyl-1-deoxynojirimycintyrosyl-phenylalaninelysope(16:1(9z)/0:0)5h-cyclopentapyrazine(2z,4'z)-2-(5-methylthio-4-penten-2-ynylidene)-1,6-dioxaspiro[4.4]non-3-ene1-cyano-2-hydroxy-3-butenel-aspartic acidallantoic acidvaldecoxib1-(gamma-glutamylamino)cyclopropanecarboxylic acidpc(18:1(9z)/14:1(9z))1-butylaminealpha-methoxy-1h-indole-3-propanoic acidepicatechin 3-o-(4-methylgallate)2-pyrrolidinone1-methyl-2-pyrrolecarboxaldehyden-nitroso-pyrrolidine3beta,6beta-dihydroxynortropane4-(3-pyridyl)-3-butenoic acid6-hydroxy-5-[(4-sulfophenyl)azo]-2-naphthalenesulfonic acidprolylphenylalaninehistidinyl-leucinecholinearabinosylhypoxanthineisovalerylglutamic acidcephalosporin c2-trans-6-cis-dodecadienal3-carbamoyl-2-phenylpropionaldehydebenzoic acid19'-hexanoyloxymytiloxanthinlettowianthinelysyl-tyrosinepsoralidinmorpholine5-ethyl-4-methyloxazolel-histidinolmelleolide mneosaxitoxintyrosyl-valineacetyl-n-formyl-5-methoxykynurenaminehistidinyl-proline6-hydroxydopamine3,4-methylenedioxybenzaldehyde2-(2-furanyl)piperidine4-(4-hydroxyphenyl)-2-butanone o-[2-galloyl-6-p-coumaroylglucoside]dl-o-phosphoserine1-methylnicotinamidecyclic amp2,6-dimethylpyrazineovalicin1-(1-pyrrolidinyl)-2-butanonepunicic acid1h-pyrrole-2-carboxaldehyde2-hydroxy-3-(3,4-dihydroxyphenyl)propanamidealanyl-threoninepropionylcarnitine1,7-dimethylguanosinemyrigalone htriethanolamine6-o-galloylsucroselactose 1(z)-4-(1-propenyl)phenolglycyl-valinen'-formylkynurenineasparaginyl-hydroxyprolinepaucinetetraacetylethylenediamine5-methyldeoxycytidinen-(gamma-glutamyl)ethanolaminephenylalanyl-alaninemetenaminethiodiglycol2-hydroxyphenethylaminephenylethyl primeverosideglucose-1,3-mannose oligosaccharide3,4-dihydroxybenzylamineglycylalanylprolylmethionylphenylalanylvalinamidebiliverdin4',5,7-trimethoxyflavonemethylpyrazinedihydrouracil1-isothiocyanato-7-(methylsulfinyl)heptane3,5-dimethyl-2-propylpyrazine1-methylpyrrole2-amino-3,8-dimethyl-3h-imidazo[4,5-f]quinoxalinecotininelinamarinisovalerylglucuronidebeta-glycerophosphoric acidmethylimidazoleacetic acid3-methyl-3h-imidazo[4,5-f]quinoxalin-2-aminemaltotetraosecis- and trans-ethyl 2,4-dimethyl-1,3-dioxolane-2-acetaten-ribosylhistidine2-pyrrolidineacetic acidn-acetylprocainamidehydroxyprolyl-gamma-glutamatesolanidinetrigonellineadrenochrome7-aminomethyl-7-carbaguanine5-aminopentanoic acid5-aminopentanalmarmesin galactosidepa(20:2(11z,14z)/22:6(4z,7z,10z,13z,16z,19z))lysopc(16:1(9z)/0:0)ethyl formatedhap(10:0)6-methyl-3,5-heptadien-2-oneglycerol 1,2-di-(9z,12z-octadecadienoate) 3-hexadecanoatelysope(0:0/20:5(5z,8z,11z,14z,17z))oxonanteninel-valineimidazoleacetic acidmukolinefalseInfluence of Culture Conditions on Metabolite Diversity in Epichloë sinensis StrainsEpichloë sinensis, associated with Festuca sinensis, exhibits rich morphological, genetic and functional diversity across different strains. To comprehensively evaluate the categories and difference in the secondary metabolites of different E. sinensis strains and thereby reveal the functional diversity, the present study analyzed the metabolites of mycelia collected from potato dextrose agar (PDA) and potato dextrose broth (PDB) cultures and fermentation broths of three different E. sinensis strains. These strains (ID1, 2, and 84C) were isolated from F. sinensis seeds collected from different locations. These three strains of E. sinensis produced a total of 1029 compounds categorized into 14 major classes in the mycelia and fermentation which included a wide range of compound structure and types, revealing E. sinensis possesses abundant metabolites. The different strains shared the similar types of metabolites which only varied in concentrations of some metabolites, indicating the similarity in metabolic function. However, types, quantity and concentrations of secondary metabolites varied in different conditions, suggesting the metabolites changed with environmental variations, as 135 unique compounds were only detected in mycelia from PDA (solid medium), while 205 exclusive metabolites were identified in PDB fermentation broth (liquid environment). Comparing metabolites of hyphae in PDA and PDB, there were a total of 100 different compounds. Comparing metabolites of hyphae in PDB and fermentation broth, there were 136 different compounds. These metabolites were mainly involved in the citrate cycle (TCA cycle) and alanine, aspartate, and glutamate metabolism.2026-01-042026-01-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