MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/m_MTBLS13898_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/m_MTBLS13898_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/a_MTBLS13898_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/s_MTBLS13898.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/a_MTBLS13898_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13898<p>Metabolites were identified using Compound Discoverer 3.3 with reference to public metabolomics databases including HMDB, ChEBI, PubChem, ChemSpider, mzCloud, and mzVault.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using a reversed-phase C18 UHPLC column with a water–methanol mobile phase containing 0.1% formic acid. A gradient elution was applied at a constant flow rate prior to mass spectrometric detection.</p>Metabolomic signatures in blastocyst spent culture medium as noninvasıve predictors of live birth: a pilot study. 10.1016/j.rbmo.2025.105442.Funda GödeBahceci Women's Health and Medical Centercell culture supernatantmass spectrometry assay<p>SCM samples were thawed and metabolites were extracted by protein precipitation using an acetonitrile:methanol:acetone mixture (8:1:1). Stable isotope-labeled phenylalanine and valine were added as internal standards. Samples were incubated at 4°C, centrifuged, and the supernatant was collected, dried under nitrogen, and reconstituted in water prior to LC-MS analysis.</p>Homo sapienshttps://www.ebi.ac.uk/metabolights/MTBLS13898Funda Gode. İzmir University of Economics. Hitay Plaza, Adalet, Anadolu Cd. No:34, 35010 Bayrakli/İzmir. fgode@bahceci.com.<p>Raw LC-MS data were converted to MS1 format and processed using Compound Discoverer 3.3 software. Data normalization was performed using pooled quality control samples and internal standards.</p>PregnancyLive birthAbortionAgefunda.gode@gmail.com<p>Spent blastocyst culture medium samples were collected from patients undergoing IVF/ICSI treatment. Embryos were cultured individually in 20 µL SAGE 1-Step™ medium supplemented with human serum albumin and covered with paraffin oil under controlled incubation conditions. After embryo transfer, spent culture media were collected using sterile pipettes and stored at −20°C until metabolomic analysis. Each sample was processed individually.</p>MetabolomicsMetabolomicsliquid chromatography mass spectrometry assayuntargeted metabolite profilingMetabolomicsliquid chromatography mass spectrometry assayuntargeted metabolite profiling<p>Mass spectrometric analysis was performed using an Orbitrap Exploris 240 mass spectrometer coupled to a UHPLC system. Data were acquired in both positive and negative electrospray ionization modes under high-resolution settings.</p>10-Undecenoic acidL-Phenylalanine(±)9-HpODE2-Aminonicotinic acidpyroglutamylglycineγ-Linolenic acidD-(-)-GlutamineN,N-Bis(2-hydroxyethyl)dodecanamide13(S)-HOTrEL-Homoserine1-[5-(4-Hydroxy-2-butanyl)-2-methyltetrahydro-2-furanyl]-4-methyl-2-pentanone2,6-di-tert-Butyl-4-methoxyphenol3-acetamidopropanalD-(+)-GlucoseL-Histidine3-{[3,4,5-Trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl]amino}-2-[(3,4,5-trihydroxytetrahydro-2H-pyran-2-yl)amino]propanenitrile (non-preferred name)Ala-GlnMyristoleic acidMFCD00209536L-(-)-methionine1-(Propyldisulfanyl)-1-(propylsulfinyl)propane4-tert-Octylphenol(2Z)-2-(2-Ethoxy-2-oxoethylidene)succinic acid2-Furoic acidNonanoic acidL-IditolMyristic acid2-Hydroxy-2-methylbutyric acid5-Hydroxy-DL-tryptophanTraumatin1-(4-Methoxyphenyl)-3-pentanone(1E)-5-(2+3:7-Hydroxy-2-propanyl)-2-methyl-8-methylene-7-oxo-1-cyclodecen-1-yl acetate(6E,9Z,12E)-6,9,12-Pentadecatrien-2-one(+/-)-Gingerol1-(5-Hydroxy-3a,4-dimethyl-3a,4,5,6,7,7a-hexahydro-1H-inden-2-yl)ethanoneDL-β-Leucine(R)-3-Hydroxy myristic acidDecanoic acidCP 47,497-C6-HomologAcrylic acidPyruvic acid(2E)-2-[(3E)-4,8-Dimethyl-10-oxo-3,7-cyclodecadien-1-ylidene]propanalDL-AlanineEthanoic anhydrideOleic acidα,α-TrehalosePentadecanoic acidBenzyl chloride4-sec-ButylphenolVS1180000MFCD11045308Levulinic acidMethyl (2E,4Z)-2,4-hexadecadienoate5-Aminovaleric acidcis-7-Hexadecenoic acid4-IndolecarbaldehydeL-(+)-Lactic acid(6Z)-9-Hydroxy-3,6,10-trimethyl-3a,4,5,8,9,10,11,11a-octahydrocyclodeca[b]furan-2(3H)-oneL-(+)-ErythruloseLinoleic acidL-TyrosineTridecylic acidHEPESMargaric acid2-Hydroxy-4-oxopentanoic acidEthyl myristate3-Hydroxypentadecanoic acidCyclopentadecanolideDiethylpyrocarbonatetrans-10-Heptadecenoic acidDinosebEicosapentaenoic acid3-Hydroxybutyric acidL-(+)-Aspartic acidfalseMetabolomic signatures in blastocyst spent culture medium as noninvasive predictors of live birth: a pilot study<p>Research Question: Can metabolomic profiling of blastocyst spent culture medium (SCM) serve as a non-invasive tool to predict live birth?</p><p>Design: This pilot study investigated the association between metabolite profiles in SCM and clinical outcomes following single blastocyst transfer. Seventy SCM samples were analyzed using untargeted liquid chromatography-mass spectrometry (LC-MS). Metabolites were identified via Human Metabolome Database (HMDB) and Compound Discoverer 3.3. Statistical analyses were performed using SPSS.</p><p>Results: Six metabolites exhibited significant differences between pregnancy and non-pregnancy groups, while ten metabolites varied between the live birth and non-live birth groups. Notably eicosapentaenoic acid (EPA), a polyunsaturated fatty acid, decreased in pregnancy cases. In contrast 1-(4-methoxyphenyl)- 3-pentanone and (2S)-2-(6-methoxynaphthalen-2-yl) propanoic acid were consistently elevated in both pregnancy and live birth groups. Additional differential metabolites included L-glutamine, pyroglutamylglycine, alanylproline, and 15,16-dihydroxyoctadecanoic acid, potentially reflecting implantation-related metabolic activity. Logistic regression and receiver operating characteristic (ROC) curve analyses demonstrated acceptable predictive performance, with an area under curve (AUC) values of 0.788 for pregnancy and 0.834 for live birth.</p><p>Conclusions: Metabolomic profiling of SCM may offer a promising noninvasive adjunct to embryo selection strategies. While these findings suggest biological relevance of several metabolites, particularly lipids and amino acid derivatives, larger studies are needed to validate predictive value and clinical applicability.</p>2026-02-132026-02-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:91151CHEBI:89809CHEBI:19092CHEBI:192106CHEBI:132810CHEBI:173243CHEBI:70976CHEBI:41218CHEBI:166517CHEBI:58354CHEBI:230729CHEBI:91399CHEBI:17405CHEBI:73393CHEBI:16870CHEBI:143097CHEBI:32664CHEBI:17169CHEBI:16716CHEBI:137246CHEBI:40957CHEBI:28579CHEBI:15354CHEBI:49284CHEBI:30260CHEBI:16924CHEBI:16296CHEBI:90873CHEBI:28123CHEBI:35702CHEBI:27454CHEBI:18377CHEBI:233526CHEBI:28787CHEBI:27943CHEBI:18086CHEBI:53405CHEBI:16092CHEBI:16643CHEBI:17053CHEBI:16283CHEBI:16015CHEBI:167175CHEBI:15971CHEBI:5738CHEBI:17016CHEBI:18347CHEBI:17295CHEBI:57926CHEBI:746917CHEBI:16414CHEBI:75144CHEBI:16146CHEBI:27915CHEBI:6802CHEBI:28092CHEBI:134543CHEBI:17752CHEBI:183735CHEBI:44920CHEBI:90355CHEBI:18049CHEBI:51148CHEBI:9444CHEBI:26271CHEBI:33135CHEBI:74077CHEBI:137944CHEBI:166486CHEBI:32220CHEBI:132244CHEBI:30817CHEBI:36812CHEBI:10136CHEBI:177850CHEBI:35045CHEBI:84441CHEBI:183868CHEBI:68572CHEBI:30845CHEBI:68454CHEBI:173533CHEBI:30322CHEBI:20067CHEBI:176993CHEBI:34442CHEBI:34445CHEBI:145234CHEBI:28171CHEBI:18308CHEBI:73788CHEBI:615597CHEBI:176577CHEBI:177420CHEBI:17061CHEBI:30813CHEBI:170067CHEBI:25017CHEBI:36610CHEBI:84849CHEBI:192076CHEBI:27913CHEBI:422CHEBI:15699CHEBI:18202CHEBI:45630CHEBI:17351CHEBI:32365CHEBI:178059CHEBI:27781CHEBI:143726CHEBI:29019CHEBI:16196CHEBI:42504CHEBI:83067CHEBI:32816CHEBI:75097CHEBI:19144CHEBI:45919CHEBI:16551CHEBI:28661CHEBI:143235CHEBI:193189CHEBI:42334ChemSpider:24765792ChemSpider:35014481ChemSpider:74886449ChemSpider:35014090ChemSpider:1228222ChemSpider:133583ChemSpider:74853410ChemSpider: 3348ChemSpider:142829ChemSpider:63972ChemSpider:4938371ChemSpider:227ChemSpider:4521424ChemSpider:769ChemSpider:14201ChemSpider:6780ChemSpider:19304ChemSpider:35032786ChemSpider: 137720ChemSpider:56318ChemSpider:8011965ChemSpider:8603910ChemSpider:35014006ChemSpider:30777171ChemSpider:30777482ChemSpider:30777352ChemSpider:35014185ChemSpider:4450466ChemSpider:198513ChemSpider:35013076ChemSpider:35013539ChemSpider:35015044ChemSpider:35014816ChemSpider:29342068ChemSpider:96749ChemSpider:2943ChemSpider:6684ChemSpider:30776875ChemSpider:200182ChemSpider:4445512ChemSpider:7758