MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13926/m_MTBLS13926_LC-MS_negative_hilic_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13926/a_MTBLS13926_LC-MS_negative_hilic_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13926/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13926/s_MTBLS13926.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13926<p>Perform retention time correction before peak area extraction using XCMS. After normalizing the total peak intensity, the processed data was imported into SIMCA-P (version 14.1, Umetrics) software to screen for differential metabolites and perform KEGG pathway analysis.</p>MetaboLightsPublicLiquid Chromatography MS - negative - hilic<p>Chromatographic separation was performed using an Agilent 1290 Infinity UHPLC system. Samples were separated using a liquid chromatography method suitable for non-volatile metabolite profiling. The mobile phases, gradient elution program, flow rate, column temperature, autosampler temperature, and injection volume were set according to the established LC-MS metabolomics method used in this study.</p>Effects of Magnesium Potassium Sulfate on Tibetan Sheep Quality and Its Volatile and Non-Volatile Metabolic Substancesate the publication title.Qinghai universityYuan Wanglongissimus dorsimass spectrometry assay<p>For metabolite extraction, 50 mg of frozen longissimus dorsi muscle sample was mixed with a pre-chilled methanol/acetonitrile/water solution at a ratio of 2:2:1 (v/v/v). The mixture was vortexed, sonicated for 30 min, and then incubated at -20°C for 10 min. The sample was centrifuged at 14,000 g for 20 min at 4°C. The supernatant was collected and dried under vacuum. For LC-MS analysis, the dried extract was reconstituted in 100 μL of acetonitrile/water solution at a ratio of 1:1 (v/v), vortexed, and centrifuged at 14,000 g for 15 min at 4°C. The supernatant was transferred to autosampler vials for analysis. Quality control samples were prepared by pooling equal aliquots from all study samples and processed using the same extraction procedure.</p>Ovis arieshttps://www.ebi.ac.uk/metabolights/MTBLS13926Yuan Wang. No. 251, Qinghai University, Chengbei District, Xining, Qinghai Province, China. 15111736236@163.com.<p>The raw data was converted to mzXML format using ProteoWizard (metabolite structure identification was performed using MetDDAA and LipDDA methods), followed by peak alignment</p>Addition amount of magnesium potassium sulfate15111736236@163.com<p>In this experiment, 120 healthy, similarly conditioned, 2-month-old weaned Tibetan lambs were selected and randomly assigned to four groups, with 30 lambs in each group. Group K served as the control group and was fed a basal diet consisting of concentrate supplement, silage oat hay, and green-dried oat hay. The concentrate supplement was mainly composed of corn, soybean meal, and premix. The experimental groups S1, S2, and S3 were fed the basal diet supplemented with PMS at concentrations of 0.2%, 0.25%, and 0.3% in the concentrate supplement, respectively. All lambs were housed in a fully enclosed barn with separate pens and an exercise yard under sheltered, sunny, dry, and well-ventilated conditions. Lambs were fed twice daily at 8:30 AM and 4:30 PM and had ad libitum access to feed and water. Longissimus dorsi muscle samples were collected after slaughter, immediately frozen in liquid nitrogen, and stored at -80°C until metabolomic analysis. Quality control samples were prepared by pooling equal aliquots from all study samples.</p>Metabolomicslongissimus dorsiAbility to Cut Meat QuestionOvis ariesuntargeted analysisBigg metabolomics database identifierpotassium magnesium sulfateexperimental blankBhyanglung, Nepal (Sheep)FlavorAB SCIEX TripleTOF 6600Agilent 1290 Infinity UHPLClongissimus dorsiAbility to Cut Meat QuestionOvis ariesuntargeted analysisBigg metabolomics database identifierpotassium magnesium sulfateexperimental blankBhyanglung, Nepal (Sheep)FlavorAB SCIEX TripleTOF 6600Agilent 1290 Infinity UHPLC<p>Mass spectrometric analysis was performed using an AB Triple TOF 6600 mass spectrometer. The instrument was operated with an electrospray ionization source for untargeted metabolomic profiling. Data were acquired in positive and/or negative ion mode according to the LC-MS acquisition method. The m/z scan range and source parameters, including ion spray voltage, source temperature, gas settings, declustering potential, and collision energy, were set according to the established metabolomics method used in this study.</p>Phosphatidylcholine lyso alkyl 16:0Trans-3'-hydroxycotinine o-.beta.-d-glucuronideL-2-hydroxyglutaric acidPyrocatechol(2-{[3-hydroxy-2-tetradecanamidooctadec-4-en-1-yl phosphonato]oxy}ethyl)trimethylazaniumEchinacosideOctanoic acid3,5-di-tert-butyl-4-hydroxybenzoic acidIsoguvacineBis(p-nitrophenyl)phosphateEthylenediaminetetraacetic acidMethyl hexadecanoate1h-indole-1-pentanoic acid, 3-(1-naphthalenylcarbonyl)-L-cysteine-glutathione disulfideKinetinDeoxycholic acid methyl esterAdenosine 5'-triphosphate (atp)Ketoleucine7-hydroxycoumarin-3-carboxylic acidFerulic acid ethyl esterProstaglandin i2AminocyclopyrachlorGlycolateCarprofenCapric acidTrans-1,4-cyclohexanedicarboxylic acidMesaconic acidD-galacturonic acidKukoamine aAdenylosuccinateGlycine1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholinecis-4-Hydroxy-D-prolineTrimellitic acid anhydrideOleic acid21-hydroxyprogesteroneN-acetyl-l-aspartic acidIsoanhydroicaritinMenthyl salicylateCis,cis-muconic acidGlutathioneFructose 1,6-diphosphate2-hydroxyisocaproic acid1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-n,n-dimethyl12-hete-[d8]Ser-SerPseudouridineUridine 5'-diphosphoglucuronic acid2'-hydroxy-2,5,6'-trimethoxychalcone1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolaminePc 36:2Pc 36:4D-(+)-galactoseEtodolac10-formyl-7,8-dihydrofolic acid2'-deoxyuridine 5'-monophosphate4-pyridoxic acidSinapyl alcoholBisdemethoxycurcuminPristanic acidCitrate3-methyl-2-oxopentanoate4'-hydroxychalcone9-oxo-10(e),12(e)-octadecadienoic acidDiflufenzopyrAcetylglycineSuccinic semialdehydeGlyceric acidL-AnserinePi 40:5Pi 40:615-ketoiloprostDihydrotachysterolArachidonic acid (peroxide free)15-cyclohexylpentanorprostaglandin f2.alpha.2',6'-dihydroxy 4'-methoxydihydrochalconeLinoleoylglycineL-threonateAla-AlaTrans-traumatic acidUridineTrans-3,5-dimethoxy-4-hydroxycinnamaldehydeOrnithine12(13)-epoxy-9z-octadecenoic acidThiosulfuric acidIsogentisinPropanoic acid, 3-[[[2-[(aminoiminomethyl)amino]-4-thiazolyl]methyl]thio]-11-(dansylamino)undecanoic acidPhe-met-arg-phe-amideIndole1-palmitoyl-2-oleoyl-phosphatidylglycerol1-palmitoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)O,o-diethyl phosphate3,7-Dimethyluric acid1-(9z,12z-octadecadienoyl)-2-hydroxy-sn-glycero-3-phosphoethanolamineIsocitric acidGlu-Met1-(1z-octadecenyl)-2-(9z-octadecenoyl)-sn-glycero-3-phosphoethanolaminePg 36:4D-xyloseThr-AlaUdp-d-glucoseGlycochenodeoxycholate5-[[17-(5,6-dihydroxy-6-methylheptan-2-yl)-3,12-dihydroxy-4,4,10,13,14-pentamethyl-2,3,5,6,7,11,12,15,16,17-decahydro-1h-cyclopenta[a]phenanthren-2-yl]oxy]-3-hydroxy-3-methyl-5-oxopentanoic acidGuanidoacetic acidLinoleic acid3,4-dihydroxymandelic acid3-(cyclohexylamino)-2-hydroxy-1-propanesulfonic acidN-(1,3-dihydroxyoctadec-4-en-2-yl)tetradecanamideDauricine17-keto-4(z),7(z),10(z),13(z),15(e),19(z)-docosahexaenoic acidBenzoic acidN-(phosphonomethyl)glycineSucrose(-)-secoisolariciresinol4-phenylphenolN-acetyl-l-methionineMannose6-hydroxymelatonin2,4-diaminobenzenesulfonic acidThr-SerZanamivir4-hydroxyquinoline6-azathymineOphthalmateS-lactoylglutathionePhytanic acid2'-hydroxy-4'-methoxyacetophenone3-hydroxymyristic acidTerephthalic acid3',4'-methylenedioxyorobolBestatinAmmelineValeric acidAbietic acid2,3-dinor-8-isoprostaglandin-f2.alpha.1-(1z-octadecenyl)-2-(5z,8z,11z,14z-eicosatetraenoyl)-sn-glycero-3-phosphoethanolaminePhenylbenzimidazolesulfonic acidEicosenoic acid18.beta.-glycyrrhetinic acidP-toluenesulfonic acid5-iodouracilVal-Tyr5-heptenoic acid, 7-[(1r,2r,3s,5s)-2-[(1e,3s)-3-(2,3-dihydro-1h-inden-2-yl)-3-hydroxy-1-propen-1-yl]-3-fluoro-5-hydroxycyclopentyl]-, (5z)-(r)-2-hydroxystearic acid6,8-dihydroxy-2,2,4,4-tetramethyl-7-(3-methylbutanoyl)-9-(2-methylpropyl)-9h-xanthene-1,3-dione1h-indole-6-carboxamide, n-hydroxy-1-[(4-methoxyphenyl)methyl]-3,5-dimethoxy-4-hydroxycinnamic acid2-phenylbutyric acid2-propylglutaric acidUdp-n-acetylglucosamineAlpha-ketoisovaleric acidAcetyl phosphateThiorphan(2-aminoethoxy)[2-[docosa-4.7.10.13.16.19-hexaenoyloxy]-3-[octadec-1-en-1-yloxy]propoxy]phosphinic acid3,4-Dihydroxy-L-phenylalanine(L-DOPA)Myo-inositolN-hydroxy-4-aminobiphenyl4,6-dinitro-o-cresolN-lauroylsarcosineUridine 5'-monophosphateTizoxanideAla-ArgVal-AsnGly-His-Lys4-aminosalicylic acidPc 32:1Flufenacet ethanesulfonic acid1,2-dioleoyl-sn-glycero-3-phospho-l-serineN-acetylputrescineD-2-phosphoglyceric acid.beta.-tocotrienol7-keto-8-aminopelargonic acidPravastatinGlycocholic acidOctadecanoic acid5-hydroxytryptopholTenofovirLeu-Leu2-cis-4-trans-abscisic acidNeobavaisoflavone3',5'-cyclic inosine monophosphate9-oxo-10e,12z,15z-octadecatrienoic acidD-myo-inositol-1,4,5-triphosphateJasmonic acid3'-.alpha.-sialyl-n-acetyllactosamine3'-hydroxyrepaglinide2,6-dihydroxy-7-methoxy-1,1,4a-trimethyl-3,4,10,10a-tetrahydro-2h-phenanthren-9-oneAla-AsnThymol-beta-d-glucoside1-palmitoyl-2-lauroyl-sn-glycero-3-phosphorylcholinePe 38:5Met-PheHeneicosanoic acidCytidine7.alpha.-hydroxy-3-oxo-4-cholestenoic acid(2r,4ar,7s,8s,8ar)-8-[(z)-5-hydroxy-3-methylpent-3-enyl]-4,4,7,8a-tetramethyl-2,3,4a,5,6,8-hexahydro-1h-naphthalene-2,7-diol1,4-butynediolOrientinGuanidinopropionic acidPhosphoserineL-dihydroorotateBeta-n-methylaminoalanineN-acetylneuraminic acid2-hydroxyanthraquinoneTaurochenodeoxycholateD-proline1-(3-pyridyl)-1-butanone-4-carboxylic acidDodecanoic acidUridine 5'-diphosphateD-mannose 6-phosphateVal-LeuHis-serCitramalateGlutathione, oxidized1-hexadecanoyl-2-sn-glycero-3-phosphateL-pyroglutamic acidCholecalciferol3-nitrophenol1-stearoyl-2-oleoyl-sn-glycero-3-phospho-(1'-sn-glycerol)Lithocholylglycine8,11-tridecadienoic acid, 13-(3-pentyl-2-oxiranyl)-, (8z,11z)-D-ribose 5-phosphateGuanosine 5'-monophosphatePelargonic acid2-heptyl-4-hydroxyquinoline n-oxide7-methylguanineNudifloramideN-Acetylglucosamine 1-phosphateQuinateCyclic gmpSarcosinePc 34:1ProgesteroneCys-GlnTaurineXanthohumolEmbelinAllantoinAmastatinPi 34:1Arachidic acidProbucol1-hexadecyl lysophosphatidic acid3-hydroxy-3-methylglutaric acidGamma-l-glutamyl-l-glutamic acid3,4-dihydroxy-5-[(2s,3r,4s,5s,6r)-3,4,5-trihydroxy-6-[[4-(2-hydroxypropan-2-yl)cyclohexene-1-carbonyl]oxymethyl]oxan-2-yl]oxybenzoic acidPhosphoenolpyruvatePc 40:7Adenosine 3'-monophosphatePe 36:2PyridoxalChalcone base + 2o, 1meo, 1prenyl or licochalcone a (not validated)Pe 36:1Thiamine monophosphateGln-gluAntibiotic k 252b(2-aminoethoxy)[3-[hexadec-1-en-1-yloxy]-2-[icosa-5.8.11.14-tetraenoyloxy]propoxy]phosphinic acid2-phosphoglycerate3-methylphosphinicopropionic acid2-amino-4,6-dihydroxypyrimidine2-naphthalenesulfonic acidCer[ns] d36:2Phosphatidylcholine 18:0-18:0 / 16:0-20:0L-aspartic acidPi 35:2L-methionine sulfoneCholesteryl sulfateUndecanoic acidBenzenethiol, 4,4'-thiobis-Ricinoleic acidIrisxanthone2'-chloro-2-hydroxy-5-methylbenzophenone2,2'-methylene-bis(6-tert-butyl)-4-ethylphenolFlurtamone1,2-dioleoyl-sn-glycero-3-phosphateInosine 5'-monophosphate3,4-dihydroxybenzaldehydePentadecanoic acid2,4-dichlorobenzoic acid[6]-gingerolL-threonineDopamineN-arachidonoyl-3-hydroxy-.gamma.-aminobutyric acidKojic acidBlood group b trisaccharideGalactinol3-hydroxybutyric acidN-acetylneuraminateTestosteroneCyclohexanesulfamic acidL-tyrosine, n-(2-benzoylphenyl)-o-[2-(methyl-2-pyridinylamino)ethyl]-FumarateN-acetyl-l-carnosine8-gingerolL-alaninamide, n-[2-[2-(hydroxyamino)-2-oxoethyl]-4-methyl-1-oxopentyl]-3-(2-naphthalenyl)-l-alanyl-Dl-a-hydroxybutyric acid7.alpha.,17.alpha.-dimethyl-5.beta.-androstane-3.alpha.,17.beta.-diol glucuronidePi 36:2L-ValineUracilPi 36:4Indolelactic acidDl-malic acidTaurocholate20-hydroxyarachidonic acidOstruthinCytidine 5'-monophosphateDL-asparagineN-stearoyltaurineValerenic acidXanthosine 5'-monophosphatePhenaceturic acidD-sorbitolIsopentenyl pyrophosphate3-[(4-chlorobenzoyl)amino]propanoic acidL-homocysteic acidOctyl gallatePe 34:34-hydroxybenzaldehydeIle-Leu4-fluorophenylacetic acidSm d34:1Methylphosphonic acidPg 40:61-oleoyl-sn-glycero-3-phosphoethanolaminePg 40:7MalateMyristic acid3-Phospho-D-glycerateTyr-Arg11-ketofusidic acidAdipic acid1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamineD-glutamineAdenosine 5'-diphosphateLeu-PheSaluamine3-hydroxyphenylacetic acid.gamma.-hydroxybutyric acid1,4-d-xylobioseHymeglusin5.alpha.-pregnan-3.alpha.,17-diol-20-one 3-sulfateL-homocitrulline3-dehydroquinic acidGln-serN6,N6,N6-Trimethyl-L-lysineGly-Ala1-oleoyl-2-hydroxy-sn-glycero-3-phospho-(1'-rac-glycerol)EthephonIsoleucineGlyphosinePyruvateD-arabinose3-hydroxyanthranilic acidGln-thr2,4-dichlorophenolHeptadecanoic acidCitraconic acidInosinePi 38:4Pi 38:5LumichromeCyclohexyl methylphosphonic acidAndrastin a6-aminouracilDihydroxyacetone phosphateCholic acidPalmitic acidAla-GlyD-aspartic acid2-deoxyribose 5-phosphateD-gluconateCaffeinePhosphoric acid1-octadecanoyl-sn-glycero-3-phospho-(1'-myo-inositol)Propionic acidNonanoic acidProstaglandin a2Deoxyadenosine2,4,6-trichlorophenolHomocitrateDl-.beta.-hydroxypalmitic acidDl-lactateGlimepirideAla-SerNorharmaneSm d42:2Hydroxyphenyllactic acid.beta.-glycerophosphate1-(3,4-dichlorophenyl)-3,3-dimethylureaPglu-glu-pro-amideMagnololXanthineDesmethylverapamil(2-aminoethoxy)[3-[hexadec-1-en-1-yloxy]-2-[hexadec-9-enoyloxy]propoxy]phosphinic acidL-gulono-1,4-lactone4-Aminobutyric acid(GABA)5-sulfosalicylic acidLeu-Glu2,4-dichloro-5-ethyl-3-methylphenolGlu-ThrSm d41:2Sulfuric acid2-ketobutyric acid(r)-butaprost (free acid)(S)-2-aminobutyric acidMaleic acidN.epsilon.-acetyl-l-lysineVal-GluIndoxyl sulfateGlufosinate3-hydroxyoctanoic acidNis(monooleoylglycero)phosphate (s,r isomer)Sm d30:1Pg 32:1Glu-SerTrp-CysCer[ns] d34:1IsocitrateFa 12:1+1oOrcinolOxypurinolPyruvaldehyde14,15-dihydroxy-5z,8z,11z-eicosatrienoic acidD-glucarateD-ribulose 1,5-bisphosphate1-hydroxy-2-naphthoic acidPe 40:6Methionine sulfoxideButaprost (free acid)20-hydroxy-4z,7z,10z,13z,16z,18e-docosahexaenoic acid3-methyl-l-histidine16-hydroxyhexadecanoic acid2-hydroxymyristic acidCelastramycin aSm d40:2Cis-9-palmitoleic acidCitramalic acid7-demethylsuberosin1-methyl-l-histidineDihydrothymineAzelaic acidL-CarnosineVal-GlnAminomethylphosphonic acid16-phenyltetranorprostaglandin f2.alpha.(z)-9,12,13-trihydroxyoctadec-15-enoic acidQuinolinateBrugierol (not validated)3-deoxy-d-glycero-d-galacto-2-nonulosonic acidDodecyl phosphateD-OrnithineMandipropamidDL-serine2-aminoadipic acidAdenosine 3',5'-cyclic monophosphateTartaric acid(2e,6e,10e)-13-[(2r)-6-hydroxy-2,8-dimethyl-3,4-dihydrochromen-2-yl]-2,6,10-trimethyltrideca-2,6,10-trienoic acidDeoxycholic acidHexadecanedioic acid, 3,3,14,14-tetramethyl-Pyridoxal phosphate2-(2,6-dihydroxy-4-methoxycarbonylbenzoyl)-3-hydroxybenzoic acidTaurolithocholic acid sulfateN-acetyl-l-phenylalaninePhe-AlaNorvaline, 5-phosphono-Cer(d18:1/18:1(9Z))falseEffects of Magnesium Potassium Sulfate on Tibetan Sheep Quality and Its Volatile and Non-Volatile Metabolic Substancesate the publication title<p>Preliminary research conducted by the team has identified that potassium and magnesium may influence the quality of Tibetan lamb meat, although the underlying mechanisms remain unclear. This study aims to examine the impact of dietary supplementation with magnesium potassium sulfate (PMS) on the carcass quality, meat quality, nutritional composition, and flavor metabolites of Tibetan sheep, while also elucidating potential mechanisms through a metabolomics approach. The findings indicate that the inclusion of 0.3% PMS in the diet significantly enhances the carcass quality of Tibetan sheep (P&lt;0.05), evidenced by increases in abdominal wall thickness and backfat thickness by 13.13% and 7.41%, respectively, compared to the control group. Furthermore, the addition of 0.25% PMS significantly elevates pH at 24 hours, a*, and intramuscular fat content, while significantly reducing pH at 45 minutes and b* (P&lt;0.05). Moreover, the supplementation of 0.25% PMS results in significantly higher concentrations of linoleic acid, linolenic acid, EPA, and DHA compared to the control group (P&lt;0.05). The findings regarding volatile compounds demonstrated that the application of PMS facilitated the production of fruity and nutty flavor compounds, including 3-methylpentanoic acid methyl ester and heptanoate methyl ester, while suppressing the accumulation of malodorous substances such as 1-hydrazino-2-propanol. Concurrently, the non-volatile compound analysis revealed that PMS primarily enhances fatty acid biosynthesis by activating the adipocyte cytokine signaling pathway. Additionally, it modulates the sphingolipid signaling pathway to synergistically regulate meat quality, thereby enhancing the flavor profile of Tibetan sheep.</p>2026-04-282026-02-22MTBLS13926