MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/m_MTBLS13964_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/m_MTBLS13964_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/s_MTBLS13964.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/a_MTBLS13964_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/a_MTBLS13964_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964/i_Investigation.txtprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS13964<p>The metabolite variables were combined by linear combination with certain weights to produce new characteristic variables, and the groups of data were categorised by the main new variables (principal components).</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>All samples were acquired by the LC-MS system followed machine orders. Firstly, all chromatographic separations were performed using a Thermo Scientifc UltiMate 3000 HPLC. An ACQUITY UPLC BEH C18 column (100 mm x 2.1 mm, 1.8 um, Waters, UK) was used for the reversed phase separation. The column oven was maintained at 35 °C. The flow rate was 0.4 mL/min and the mobile phase consisted of solvent A (water, 0.1% formic acid) and solvent B (acetonitrile, 0.1% formic acid). Gradient elution conditions were set as follows: 0.0-0.5 min, 5% B, 0.5-7.0 min, 5-100% B; 7.0-8.0 min, 100% B; 8.0-8.1 min, 100-5% B; 8 1-10.0 min, 5% B. The injection volume for each sample was 4 μL.</p>Changes in the Abundance of the Fecal Microbiota are Implicated in the Pathogenesis of Diarrhea in Yak Calves.Shaanxi normal universityMantang Gechyme materialmass spectrometry assay<p>The collected samples were thawed on ice, and metabolite were extracted with 50% methanol buffer. Briefly, 20 uL of sample was extracted with 120 uL of precooled 50% methanol, vortexed for 1 min, and incubated at room temperature for 10 min, the extraction mixture was then storedovernight at -20 °C, After centrifugation at 4000 x g for 20 min, the supernatants were transferredinto new 96-well plates. The samples were stored at -80 °C prior to the LC-MS analysis. Inaddition, pooled OC samples were also prepared by combining 10 j of each extraction mixture</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS13964Zhu Yanbin. 535922126@qq.com.Zhu Yanbin. Tibet Academy of Agricultural and Animal Husbandry Sciences. No.620 at Xi Chang'an Street Xi'an province. 535922126@qq.com.<p>The raw mass spectrometry downcomer files were converted to mzXML file format by the MSConvert tool in the Proteowizard software package (v3.0.8789). Peak detection, peak filtering and peak alignment were processed using the R XCMS software package, with parameters such as bw=2, ppm=15, peakwidth=c(5, 30), mzwid=0.015, mzdiff=0.01 and method='centWave' to obtain a Substance quantification list.Support vector regression correction based on QC samples was used to eliminate systematic errors. Substances with Coefficient of Variance (CV) less than 30% in the QC samples were then retained for subsequent analysis.</p>SexTimepoint535922126@qq.com<p>Mice were fed barley for 7, 21 and 35 days and hippocampus and intestinal contents were taken for metabolic analyses.</p>MetabolomicsIntestineRattus norvegicusuntargeted metabolite profilingIntestineRattus norvegicusuntargeted metabolite profiling<p>A high-resolution tandem mass spectrometer TripleTOF6600plus (SClEX, Framingham, MA, USA) was used to detect metabolites eluted form the column. The Q-TOF was operated in both positive and negative ion modes. The curtain gas was set 30 PSl, lon source gasl was set 60 PSl, lon source gas2 was set 60 PSl and an interface heater temperature was 650 °C. For positive ionmode, the lonspray voltage floating were set at 5000 V, respectively. For negative ion mode, the lonspray voltage floating were set at -4500 V, respectively.</p>(-)-.alpha.-kainic acidTaurineTrans-cinnamic acid7,8-dihydrobiopterin2(1h)-pyridinoneMilnacipranSolanidineEthylenediaminetetraacetic acidDihydrofolic acidStearoyl ethanolamideGamma-l-glutamyl-l-glutamic acidAtropine1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholineL-HistidineTrigonellinePyridoxalDocosatrienoic acidGly-LeuNg,ng-dimethyl-l-arginineHispidulinD-erythro-sphingosine-1-phosphatePerchloroterephthalic acidPentostatinAmpicillin2'-deoxycytidine5-L-Glutamyl-L-alanine4-oxo-2-nonenal2-linoleoyl-1-palmitoyl-sn-glycero-3-phosphoethanolamineFeruloyl tyramineL-methioninePrilocaineD-Mannose17alpha-hydroxyprogesteroneMyristoyl-l-carnitineD-erythro-sphinganine-1-phosphateDaunorubicinArginine1-arachidoyl-2-hydroxy-sn-glycero-3-phosphocholineGlu-ArgL-NorleucineN-acetyl-l-aspartic acidTriethanolamineDicyclomineCyclizineOleoyl-l-carnitineDL-valineNiacinamideCoenzyme q21-stearoyl-2-docosahexaenoyl-sn-glycerolPhenylalanineMelamine4-hydroxy-3-methoxybenzyl alcohol7.alpha.-hydroxy-4-cholesten-3-oneL-carnitineL-theanine5-hydroxyflavone.beta.-naphtholPentazocinePantothenateN-lauroyl-d-erythro-sphinganineIsophoroneL-homoarginineOleamide(+)-catechinHexylamine3-phosphonoalanineSarafloxacinAmprenavir5-aminolevulinic acidPantothenic acidL-leucyl-l-proline1-Stearoyl-2-hydroxy-sn-glycero-3-phosphocholineHydrocinnamic acid ethyl esterCuminaldehydePraziquantelCis-13-docosenoic acid2-hydroxyestradiolTrimethoprimHarmineN-acetylhistamineCreatineGossypolN-phenyl-2-naphthylamineD-asparagineAcetyleugenolPhytosphingosinePro-hypTropicamideOctanoylcarnitineAflatoxin b1Glu-HisG-guanidinobutyrateCytosinePropranololDL-cysteineOctaethylene glycol1,2-dioleoyl-sn-glycero-3-phosphatidylcholine2-hydroxyatrazine3-aminobenzoic acid2-palmitoyl-rac-glycerolLevamisoleBenzyl alcohol1-stearoyl-2-linoleoyl-sn-glycero-3-phosphocholine1-palmitoyl-sn-glycero-3-phosphocholinePalmitoyl sphingomyelinMethylguanidinePyridineSulfamethazineL-saccharopine1-(1z-octadecenyl)-2-(5z,8z,11z,14z-eicosatetraenoyl)-sn-glycero-3-phosphocholineHypoxanthineSialyl lewis aBisphenol a diglycidyl etherCinoxacinPalmitoyl ethanolamidePindolol16.alpha.-hydroxyestroneTrans-zeatinCyprodinilD-glutaminePermethrinN6-methyl-l-lysineNevirapineAndrostan-3-ol-17-one 3-glucuronideEquolLomefloxacin1,6-anhydro-.beta.-d-glucoseCysteic acidN-stearoylsphinganine1-stearoyl-2-linoleoyl-sn-glycero-3-phospho-l-serineN-myristoylsphinganineDiazinonN-oleoyl-d-erythro-sphingosylphosphorylcholineL-homocitrullineBeclomethasone1,2-dioleoyl-sn-glycerolPyroquilonDiethanolamine1-Stearoyl-2-arachidonoyl-sn-glycerolN-acetyl-.beta.-d-mannosamine1,2-dihexadecanoyl-sn-glycero-3-phosphocholinePhenylacetyl-l-glutamineHarmalineEstrone sulfate.gamma.-aminobutyric acidEnoxacinAdenine2-ketohexanoic acid(+)-.alpha.-tocopherolPterostilbene2-arachidonoylglycerol1-behenoyl-2-hydroxy-sn-glycero-3-phosphocholineCortisone1-Stearoyl-sn-glycerol 3-phosphocholine(LPC(18:0))1,2-dilinoleoyl-sn-glycero-3-phosphoethanolamineCyromazineCocaineDimethylglycinePro-Trp5.alpha.-cholest-7-en-3.beta.-ol.gamma.-l-glu-.epsilon.-l-lys4-(methylnitrosamino)-1-(3-pyridyl)-1-butanolPrimaquineN-acetyl-d-lactosamineFamciclovirTrachelanthineIsoproterenol4-cholestenoneCarnosolStearoyl-l-carnitineHistidineLntNormeperidineAnserineCortexoloneS-lactoylglutathioneSphingosineDisulfiram1-methylhistidineLeukotriene b4 dimethylamideChlorhexidine(-)-riboflavin3-Methylhistidine1-(1z-octadecenyl)-2-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glycero-3-phosphocholine3h-imidazo(4,5-f)quinoline, 2-amino-3-methyl-MetyraponePhenylbutazoneBeta-estradiolTelmisartan1-(1z-hexadecenyl)-sn-glycero-3-phosphocholineIsobutyryl-l-carnitine4-methyl-1h-pyrazoleLysineAntipyrine5-methylcytosineLinoleoylcarnitinePerindoprilTrimethylamine n-oxideGlycerophosphocholineDiethyl phthalate1-Aminocyclopropanecarboxylic acid4,6-diamino-5-formamidopyrimidineTetraethylene glycolLauroyl-l-carnitine1-myristoyl-2-palmitoyl-sn-glycero-3-phosphocholineMeperidine1-myristoyl-sn-glycero-3-phosphocholine1-pentadecanoyl-sn-glycero-3-phosphocholineIndoleacetic acidDiethylcarbamazine2-amino-1-phenylethanolDidodecyl 3,3'-thiodipropionate4-hydroxycyclophosphamideCreatinineDexpanthenolFlunisolideL-propionylcarnitine1-palmitoyl-2-linoleoyl-sn-glycero-3-phosphocholine.beta.-ecdysoneN-nitrosodimethylamineDiacetylL-palmitoylcarnitineAll-trans-4-ketoretinoic acidKetamineL-GlutamineD-glucosamine 6-phosphateNorcodeineN-palmitoyl-d-sphingosineStachydrineCholineAcetylcholine2-hydroxyestroneHexaethylene glycolAtrazineMethaqualoneN-acetylputrescineTrans-4-(aminomethyl)cyclohexanecarboxylic acidMalonyl-l-carnitine1-stearoyl-2-arachidonoyl-sn-glycero-3-phospho-(1'-myo-inositol)5-phospho-d-ribose 1-diphosphate.delta.-hexalactoneLabetalolSofalconeDesisopropyldisopyramideSorbitane monopalmitate - polysorbate 40 in-source fragmentGamma-glu-gluChenodeoxycholate1-(1z-octadecenyl)-sn-glycero-3-phosphocholineTenofovirDiosmetinBisphenol a.gamma.-glu-cysBetaineTramadol3-methoxytyramine1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine1,2-didocosahexaenoyl-sn-glycero-3-phosphocholineN.epsilon.-acetyl-l-lysineHesperetinMenadionePiperettineL-pipecolic acidCidofovirPhosphorylcholine5-methoxytryptophanL-hydroxyarginineN,n-dimethylformamideZaleplonTiclopidineHydroquinone3-dehydrocarnitineN-nervonoyl-d-erythro-sphingosylphosphorylcholineQuinidineSolasodineAtorvastatinAgomelatinePicrotoxinin(r)-butyrylcarnitineL-GlutamateDesferrioxamineFenamiphosCafestolN,n,n-trimethyllysineAlfentanylN-methyl-l-glutamic acidLeucine3-methyl-l-histidineHydrocortisoneOxazepamN-methyl-l-alanineGenipin1-methyl-l-histidinePalmitamide1-heptadecanoyl-sn-glycero-3-phosphocholineTrioxsalenCurcuminPiperidineGlutathione, oxidized6-benzylaminopurinegamma-L-Glutamyl-L-phenylalanineHalosulfuron-methylL-canavanineCholecalciferolLithocholylglycineArecaidineTerfenadine1-o-hexadecyl-2-o-(4z,7z,10z,13z,16z,19z-docosahexaenoyl)-sn-glyceryl-3-phosphorylcholineQuercetin 3'-methyl etherN-acetylhistidineXylitol7-methylguanineNudifloramideQuinateDeoxycytidineIsatinGlycodeoxycholic acidSarcosine2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholineIsoeugenyl acetateDaidzeinDl-2-aminocaprylic acidfalseChanges in the Abundance of the Fecal Microbiota are Implicated in the Pathogenesis of Diarrhea in Yak Calves<p>The yak, an economically vital species on the Qinghai-Tibet Plateau, faces a significant challenge with diarrhea during the calf stage. However, studies about diarrhea of yak calves were few. Due to the close link between diarrhea and intestinal microbiota. Therefore, this study analyzed the fecal microbiota in diarrheic and healthy yak calves, and using a pseudo germ-free (PGF) mouse model to investigate the causes for diarrhea. This study identified microbial dysbiosis in diarrheic yak calves, characterized by significant enrichment of Ruminococcus gnavus and Streptococcus.</p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