MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/m_MTBLS14011_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/m_MTBLS14011_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/a_MTBLS14011_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/s_MTBLS14011.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011/a_MTBLS14011_LC-MS_negative_reverse-phase_metabolite_profiling.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14011And then peakswerematched with the mzCloud(https://www.mzcloud.org/), mzVault and MassList database to obtain the accuratequalitative and relative quantitative results.Statistical analyses were performed usingthe statistical software R(R version R-3.4.3),Python (Python 2.7.6 version) andCentOS (CentOS release 6.6),When data were not normally distributed, normaltransformations were attempted using of area normalization methodMetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseUHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (Thermo Fisher, Germany) coupled with an Orbitrap Q Exactive™ HF-X mass spectrometer (Thermo Fisher, Germany) at Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypersil Gold column (100 × 2.1 mm, 1.9 µm) using a 17-minute linear gradient at a flow rate of 0.2 mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in water) and eluent B (methanol). The eluents for the negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (methanol). The solvent gradient was set as follows: 2% B, 1.5 min; 2–100% B, 3 min; 100% B, 10 min; 100–2% B, 10.1 min; 2% B, 12 min.Deletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses.Capital Medical Universityyuqing sunCulture Supernatantmass spectrometry assayThe samples (100 uL) were placed in the EP tubes and resuspended with prechilled 80%methanol by well vortex. Then the samples were incubated on ice for 5 min andcentrifuged at 15,000 g, 4'C for 20 min. Some of supernatant was diluted to finalconcentration containing 53% methanol by LC-MS grade water.The samples weresubsequently transferred to a fresh Eppendorf tube and then were centrifuged at15000 g, 4'C for 20 min. Finally, the supernatant was injected into the LC-MS/MSsystem analysis[2-3]Aspergillus fumigatushttps://www.ebi.ac.uk/metabolights/MTBLS14011Yuqing Sun. Department for Disinfection and Infection Control, Chinese PLA Center for Disease Control and Prevention. sunyq0519@163.com.The raw data files generated by UHPLC-MS/MS were processed using the CompoundDiscoverer 3.1 (CD3.1, ThermoFisher) to perform peak alignment, peak picking, andquantitation for each metabolite. The main parameterswere set as follows: retentiontime tolerance, 0.2 minutes; actual mass tolerance, Sppm; signal intensity tolerance30%; signalnoise ratio, 3; and minimum intensity, et al. After that, peak intensitieswere normalized to the total spectral intensity.The normalized data was used topredict the molecular formula based on additive ions, molecular ion peaks andions.Treatmentsunyq0519@163.comAfter culturing A. fumigatus for 24 hours, the culture supernatant was collected by centrifugation at 8000 rpm for 10 minutes and immediately frozen in liquid nitrogen.MetabolomicsgliotoxinAspergillus fumigatusImmunityuntargeted metabolite profilinggliotoxinAspergillus fumigatusImmunityuntargeted metabolite profilingQExactiveTM HlF-X mass spectrometer was operated in positive/negative polarity modewith spray voltage of 3.5 kV, capillary temperature of 320°C, sheath gas flow rate of35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heatertemperature of350°C3-(4-ethoxyphenyl)-2-(tetrahydro-1H-pyrrol-1-ylcarbonyl)acrylonitrile1-morpholino-3-(4-nitrophenoxy)propan-2-olNicotinamide adenine dinucleotide (NAD+)Prostaglandin G2RiboflavinMonolaurinL-PipecolateLimonin13(S)-HOTrEN-Desmethyltramadol2-[(3S)-1-(1H-Indol-3-ylmethyl)-3-pyrrolidinyl]-1H-benzimidazoleKinetinNormorphineL-TyrosinemethylesterProstaglandin H1(+)-ar-TurmeroneVasicinoneApocyninBiotin2-[4-(2,4-dimethylphenyl)-1,3-thiazol-2-yl]pyrazineD-RaffinoseTropolone¦Á-Aspartylphenylalanine6-Methoxy-2-naphthoic acidBialaphos6-methoxy-2,3,4,9-tetrahydro-1H-beta-carbolin-1-one3-Hydroxyanthranilic acid2-(3,4-dimethoxyphenyl)-N-(4-morpholinophenyl)acetamideN-Acetyl-D-tryptophan3-(5,7-dimethoxy-4-oxo-4H-chromen-2-yl)propanoic acid4-HydroxybenzaldehydeUracil 1-beta-D-arabinofuranoside2-[1-oxophthalazin-2(1H)-yl]ethyl thiophene-2-carboxylate7-(2-aminophenyl)heptanoic acidN6-Me-dAcis-4-Hydroxy-D-prolineL-AsparagineParacetamolGuanosine monophosphateRicinine3-(propan-2-yl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione2-hydroxy-6-[(8Z,11Z)-pentadeca-8,11,14-trien-1-yl]benzoic acid1-[(2,5-dichloro-3-thienyl)sulfonyl]-4-phenethyl-1,4-diazepaneCorey Lactone Diol6-MethylquinolineGlutaric AcidOxohongdenafilCytidine 5'-monophosphate (hydrate)D-(+)-MaltoseMaltopentaose1-(4-hydroxyphenyl)propane-1,2-diolN4-[5-(2-pyridyl)-2-thienyl]morpholine-4-carboxamideIsophoroneIsoferulic acid4-Methyl-5-thiazoleethanol1,4-Dihydro-1-Methyl-4-Oxo-3-PyridinecarboxamideN6-Methyladenine(+)-alpha-Lipoic acid2-[(carboxymethyl)(methyl)amino]-5-methoxybenzoic acidUR-144 N-(5-Bromopentyl) analogN-Acetylneuraminic acid4-Pyridoxic acidPantothenic acid5-[4-(phenylsulfonyl)phenyl]-1H-pyrazoleVindolineProtopine4-(cyclohexylmethyl)-6-(2-thienyl)-2,3-dihydropyridazin-3-one hydrateN1-(3,5-dichlorophenyl)-3-amino-3-hydroxyiminopropanamide2-oxopiperidine-3-carbohydrazidePheniramine N-oxide3-hydroxy-2-octylpentanedioic acidL-OrnithineD-Glucosamine 6-phosphate5'-S-Methyl-5'-thioadenosine5,6-dimethyl-4-oxo-4H-pyran-2-carboxylic acidN2-tetrahydrofuran-2-ylmethyl-4-(4-fluorophenyl)-1,3-thiazol-2-amine2-{[(2-{[(2-hydroxyphenyl)methylene]amino}ethyl)imino]methyl}phenol5-Fluoro-2-[(3S)-1-(2-fluorobenzyl)-3-pyrrolidinyl]-1H-benzimidazoleDL-Lysine5,6-Dihydroxyindole-2-Carboxylic AcidN-Formylkynurenine7-(3,4-dihydroxyphenyl)-5-hydroxy-1-(4-hydroxyphenyl)heptan-3-oneNeosaxitoxinBoc-beta-cyano-L-alanine2-furyl[4-(1H-indol-4-yl)piperazino]methanone4-[1-(dimethylamino)ethylidene]-2-phenyl-1,3-oxazol-5(4H)-oneOxaceprol4-oxododecanedioic acidN'2-[4-(trifluoromethyl)benzylidene]-3-aminopyrazine-2-carbohydrazideAlanyltyrosine1-Phenyl-3-methyl-5-pyrazolone(12Z)-9,10,11-trihydroxyoctadec-12-enoic acidTKK1-MethylguanineUMP2-PhenylglycineD-Gluconic acidFmoc-L-IsoleucineFPH3-(2-Naphthyl)-D-AlanineVitamin B2HomotaurineIndoleDulcitolProlineOctadeca-11E,13E,15Z-trienoic acid¦´-L-Glutamyl-L-glutamic acidProstaglandin A3DL-Arginine4-(2,3-dihydro-1,4-benzodioxin-6-yl)butanoic acid6-Keto-prostaglandin f1alphaAsp-Glu2-chloro-6-(1,4-thiazinan-4-yl)benzonitrile3-NitrotyrosineL-HydroxylysineZeatin-7-N-glucosideo-CresolThiamineGly-Tyr-Ala2-(2,6-dimethoxyphenyl)-5,6-dimethoxy-4H-chromen-4-one6-(7-methyloctyl)-1H,3H,4H,6H-furo[3,4-c]furan-1-oneVQHCycloheximideN1-(2-amino-2-oxoethyl)-2-(isopropylthio)acetamideSKKTMKL-TyrosineL-DopaAdipamide3-oxoindane-1-carboxylic acid2-[5-(2-hydroxypropyl)oxolan-2-yl]propanoic acidEPH2-Isopropylmalate(-)-Caryophyllene oxideEPKL-Phenylalanine2,6-dimethyl-4-(3-thienyl)pyridine-3,5-dicarboxylic acid4-(1H-pyrazol-1-yl)-N,N-bis(2-pyridinylmethyl)benzenesulfonamide5-Methylcytosine3-hydroxy-3-methylpentanedioic acidN1-isopropyl-2-(phenylthio)benzamide4-methoxy-6-(prop-2-en-1-yl)-2H-1,3-benzodioxoleCLOFAZIMINETerephthalic AcidN6-Acetyl-L-LysineThioguanineIsoproterenol4-hydroxy-3-(3-methylbut-2-en-1-yl)benzoic acid2,5-bis(4-hydroxy-3-methoxyphenyl)-3,4-dimethyloxolan-3-olN1-prop-2-ynyl-2,5-di(2,2,2-trifluoroethoxy)benzene-1-sulfonamideSpermidineL-(-)-MethionineS-(Methyl)Glutathione5-allyl-6-methyl-2-(1,4-thiazinan-4-yl)pyrimidin-4-ol3-(4-nitrophenyl)[1,2,3]triazolo[1,5-a]quinazolin-5-aminefarensyl diphosphategamma-GlutamylmethionineAflatoxin M1Threonine4-phenoxyphenyl 4-hydroxypiperidine-1-carboxylateRauwolscineHydroxyglutaric acidSorbic acidPropionylcarnitine2-ethyl-2-(3-methoxyphenyl)cyclohexanone oximeL-Iditol4-decyl-3-hydroxy-5-oxooxolane-2,3-dicarboxylic acid12,13-EODE5-acetyl-2,6-dimethyl-1,2,3,4-tetrahydropyridin-4-oneUreidoisobutyric Acidp-Mentha-1,3,8-triene3-amino-2-phenyl-2H-pyrazolo[4,3-c]pyridine-4,6-diolObscurolide A1DL-TryptophanN-{4-[4-(3-chlorophenyl)piperazino]butyl}-4-methoxybenzamideTrehalose4-methoxy-6-[2-(4-methoxyphenyl)ethyl]-2H-pyran-2-one2,3,4-Trihydroxybenzoic acidN-[2,5-bis(2,2,2-trifluoroethoxy)benzoyl]-N'-(4-methoxyphenyl)ureaTPHL-Glutamic acid monosodium saltGuanine2-NaphtholN-Acetyl-L-glutamine(¡À)11(12)-EET2,6-Dihydroxybenzoic acid2-[2-oxo-2-(2-pyridylamino)ethoxy]acetic acidAcetophenoneAdenosine 5'-DiphosphateN-Acetyl-aspartic acidCreatinine4-Methoxycinnamic Acid(3S,9aS)-3-benzyl-octahydro-1H-pyrido[1,2-a]pyrazin-1-one2-{4-[(trifluoromethyl)thio]phenoxy}ethanohydrazideAcetyl-L-carnitineGuanosine 5'-diphosphate (GDP)N6-Me-Adenosine7-MethylguanineBicyclo prostaglandin E26-(3-hydroxybutan-2-yl)-5-(hydroxymethyl)-4-methoxy-2H-pyran-2-onetrans-3-Indoleacrylic acidLL-2,6-Diaminopimelate3-(4-hydroxy-3-methoxyphenyl)propanoic acidDRH2-Amino-1,3,4-octadecanetrioltetranor-12(R)-HETEProstaglandin K2N-[2-chloro-6-(trifluoromethoxy)phenyl]-2,2-dimethylpropanamideN'2-cinnamoyl-3-(1H-pyrrol-1-yl)thiophene-2-carbohydrazideAdenosine 3'5'-cyclic monophosphateIndole-3-acrylic acid2-(2-methoxyphenyl)-N-(2-quinolinyl)acetamidemesityl(piperidin-4-yl)methanone hydrochloride4-(4-methoxyphenyl)-2-(2-thienyl)pyrimidineBetaineN-MethylhydantoinLevodopaN-(2,6-Dimethylphenyl)-N-(methoxyacetyl) alaninedimethyl N~1~,N~4~-dicyanotetrahydropyrazine-1,4-dicarbimidothioateAsp-PheEnrofloxacinCytidine5-(tert-butyl)-2-methyl-N-(5-methyl-3-isoxazolyl)-3-furamide3-hydroxy-4-methoxy-[2,2'-bipyridine]-6-carboxamide3-(2-thienyl)-1,2,4-oxadiazole-5-carbohydrazide2-{[(4,5-dimethoxy-2-nitrophenethyl)imino]methyl}phenolN-P-Coumaroyl SpermidineIsoquinolineP-Aminohippuric AcidMonobutyl phthalateANH2'-Deoxyadenosine-5'-monophosphate2-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]pyridin-3-amineN-BenzylformamideCys-GlyN3,N4-Dimethyl-L-arginine7-methyl-3-nitroimidazo[1,2-a]pyridineBz-RS-ISer(3-Ph)-Ome2-Benzyl-5-[(3S)-1-isopropyl-3-pyrrolidinyl]-1,3,4-oxadiazole1,4-dihydroxy-1,4-dimethyl-7-(propan-2-ylidene)-decahydroazulen-6-one4-Oxoretinol3-[3,5-bis(trifluoromethyl)phenyl]-N'-hydroxyprop-2-enimidamideQNK3,6-dibenzyl-1,2-dihydro-1,2,4,5-tetraazineDL-5-Methoxytryptophan(2R)-5-hydroxy-7-methoxy-2-phenyl-3,4-dihydro-2H-1-benzopyran-4-oneL-Adrenalinecis-Aconitic acidN alpha-Acetyl-L-ArginineN-{4-[(2R,3R)-3-(Hydroxymethyl)-5-oxo-2-morpholinyl]phenyl}acetamide¦Á-Lapachone3-(dimethylamino)-1-[4-(phenylsulfonyl)phenyl]prop-2-en-1-oneThymine9,10-Dihome3-{[(4-fluorophenyl)sulfonyl]amino}-5-phenylthiophene-2-carboxamideNootkatone1-Methyladenosine6-(4-phenylpiperazino)hexanoic acid hydrochloride4-Hydroxy-6-methyl-2-pyroneAPKAnthranilic acid(5S)-5-hydroxy-1,7-diphenylheptan-3-oneN(6)-OH-Me-AdenosineL-Cysteine-glutathione gisulfideGamma-Glu-Leu7-Hydroxy-3,4-dihydrocarbostyrilL-arginine3-Hydroxy-3-methylbutanoic acid5-methoxy-8,8-dimethyl-2-phenyl-4H,8H-pyrano[2,3-h]chromen-4-one5-(6-hydroxy-6-methyloctyl)-2,5-dihydrofuran-2-one4-cyclopropyl-6-methoxy-1,3,5-triazin-2-amineo-Toluic Acid4-Guanidinobutyric acidL-Saccharopine5-Hydroxytryptophan9-KODE3-(5-phenyl-1,3-oxazol-2-yl)-4-(trifluoromethyl)pyridine4-(2,3-dihydro-1H-indol-1-yl)-1-phenyl-1H-pyrazolo[3,4-d]pyrimidine4-Ethylbenzaldehyde3-Indoleacrylic acidTrigonellineMethylmalonate3-(2,4-dichloroanilino)-1-(4-methoxyphenyl)prop-2-en-1-oneMethyl 3-indolyacetateL-ErgothioneineN-Acetyl-Asp-Glu1-ethyl 4-(2-oxo-1,2-diphenylethyl) succinate3-Methoxy prostaglandin F1¦Á5,8-dihydroxy-10-methyl-5,8,9,10-tetrahydro-2H-oxecin-2-one5-Hydroxyindole-3-acetic acidN1-(4-cyclohexylphenyl)-2-[(4-methylphenyl)thio]acetamideN-lactoyl-phenylalanineDeisopropylatrazineMAG (18:3)N-PropionylglycineN1-methyl-5-methoxy-2-({2-[(methylamino)carbonyl]phenyl}thio)benzamideN6-Succinyl AdenosineStyreneDl-2-Amino-3-phosphonopropionic acid7-MethylguanosineL-5-Hydroxytryptophan2-[4-(tert-butyl)phenyl]-5-(2-nitrophenyl)-1,3,4-oxadiazole2-[(1,3-thiazol-2-ylamino)methylidene]malononitrileD-threo-Isocitric acid2,6-Di-tert-butyl-1,4-benzoquinone2,3-dihydroxypropyl 12-methyltridecanoatePPH5-oxoprolinePPKCystineL-Glutathione oxidized3-Methoxytyramineethyl 4-bromo-1,5-dimethyl-1H-pyrazole-3-carboxylateL-Tryptophan4-ButylresorcinolLysops 22:6N1-(1-benzyl-4-piperidyl)-4-chlorobenzene-1-sulfonamideGlu-Gln5-oxo-3-phenyl-5-(2-quinolinylamino)pentanoic acidPhenylacetylglycinedCMP2-Methylpentanedioic acidL-Leucyl-L-alanine Hydrate2-Hydroxy-6-AminopurinePsoralidin3-{[(3S)-3-(1,3-Benzothiazol-2-yl)-1-pyrrolidinyl]methyl}benzonitrileO-DesmethylnaproxenAKB48 N-(4-fluorobenzyl) analogLeucylprolineTangeritinN1-[4-hydroxy-6-(methoxymethyl)pyrimidin-2-yl]acetamideL-ValineDY131UracilMelanin1-ethyl-4-(2-phenylethynyl)piperidin-4-ol8-HydroxyquinolineLuotonin A5-hydroxy-4-methoxy-5,6-dihydro-2H-pyran-2-onedUTPCuminaldehyde(2,6-dimethylmorpholino)(1-methyl-5-nitro-1H-pyrazol-4-yl)methanoneMaltotetraoseMorphinealprazolam-d54-(4-nitrophenylazo)anilineDL-CitrullineOctopine3,4-Dihydroxyphenylpropionic acid16-Heptadecyne-1,2,4-triolCytosine3-(1-naphthylmethylidene)indolin-2-one2-[(butylamino)(imino)methyl]-1-oxohydrazinium-1-olateN-(1,3-benzodioxol-5-ylmethyl)-N'-(1-benzothiophen-3-yl)urea4-(trifluoromethyl)nicotinic acidL-CystineDL-CarnitineN3-(2-morpholinophenyl)-2-oxo-2H-chromene-3-carboxamideCoumarin7-(2-hydroxypropan-2-yl)-1,4a-dimethyl-decahydronaphthalen-1-olPDMPNicotinate ribonucleosideSpermineN-acetyl-L-ornithineHypoxanthinePalmitoyl ethanolamideCalcium D-PanthotenateN-Acetyl-DL-phenylalanineethyl 4-(6-bromo-2-methyl-4-oxo-3,4-dihydroquinazolin-3-yl)benzoate3-isopropoxy-4-morpholinocyclobut-3-ene-1,2-dioneO-Aceyl-L-Serine4-(4-methoxyphenyl)-2-(methylthio)pyrimidine2,6-DihydroxypurineN-glycyl-L-proline2-Methoxybenzaldehyde7,8-Dihydrofolate2-(4-methoxyphenyl)-3-(1H-pyrazol-4-yl)acrylonitrileTriiodothyronine4-(3-methoxyphenyl)-4-methyl-2,6-dioxopiperidine-3,5-dicarbonitrile5-HydroxyindoleDiacetoxyscirpenol5-MethyluridineL-Glutamic acid7-chloro-4-piperidinoquinolineHaloperidol metabolite IIPalmitoleic Acid2-(tert-butyl)-6,7-dimethoxy-4H-3,1-benzoxazin-4-oneAdenineD-Ala-D-AlaN2-Acetyl-L-ornithine4-oxo-4-[(1-phenylethyl)amino]but-2-enoic aciddAMP2-(2-thienyl)-4H-chromen-4-oneGly-Pheethyl 4-methyl-2-(1H-pyrrol-1-yl)-1,3-thiazole-5-carboxylate6-cyclohex-3-enyl-5-nitropiperidin-2-oneN1-(1H-indol-4-yl)cyclohexane-1-carboxamidePurineDl-3-HydroxynorvalineNaratriptanLNH1-(4-bromophenyl)-2-phenylethan-1-oneDesthiobiotinTetranor-12(S)-HETEPseurotin AMezlocillinN-Palmitoyl taurine(2R)-2-[(2R,5S)-5-[(2S)-2-hydroxybutyl]oxolan-2-yl]propanoic acidAla-Gln4-(2-chloro-6-fluorostyryl)pyrimidin-2-amineTyramine(6E)-7-(2H-1,3-benzodioxol-5-yl)-1-(piperidin-1-yl)hept-6-en-1-one(¡À)8-HEPESedanolideDeoxyadenosine5,6-dimethoxy-2-(2-methoxyphenyl)-4H-chromen-4-oneD-Galactosamine4-Phenyl-3-buten-2-oneAsp-Phe methyl ester1-benzyl-1,2,3,5-tetrahydroimidazo[2,1-b]quinazolin-5-oneNicotinamideMethyl dihydrojasmonateAzetidine-2-carboxylic acidQuinoline-4-carboxylic acidN-(4-butyl-2-methylphenyl)-N'-[4-(4-methylpiperazino)phenyl]ureaSkatole6,6-dimethyl-4-piperidino-5,6-dihydro-2H-thiine-2-thione4-Hydroxy-3-methylbenzoic acid2-Aminobenzenesulfonic acid4-methyl-5-oxo-2-pentyl-2,5-dihydrofuran-3-carboxylic acidCystaminePyridoxamine5-Methyl-8-nitro-3-spirocyclohexyl-2,3,4,5-tetrahydro-1H-2-benzazepine12-Oxo phytodienoic acid4-(tert-butyl)phenyl 3,5-dimethylisoxazole-4-carboxylateL-Pyroglutamic acidDl-ThreitolN-Acetylhistidine2-Phenylethylamine(¡À)-Abscisic acidPyrithioxinAndrosterone6-Aminonicotinamide¦Â-Nicotinamide mononucleotide4-phenyl-2-[(3-pyridylamino)methylidene]cyclohexane-1,3-dioneUR-144 N-Heptyl analog3-(3,4-Dihydroxyphenyl)-2-MethylalanineADP-riboseNobiletinCholine2,4-Dihydroxybenzoic acidAcetylcholine5-OxoETEAdenosine 5'-monophosphate2-(3,4-dimethoxyphenyl)ethanamineOctadecanamineNicotinic acid2,4-di(2,3-dihydro-1,4-benzodioxin-6-yl)-1,3-thiazoleVal-SerNicotinamide N-oxideYLK3-Phosphoglyceric acidGelsemineInositol7¦Á-Hydroxytestosteronelipoamide3-MethylindoleLinolelaidic Acid (C18:2N6T)S-AdenosylhomocysteineTetracyclineHistamine3'-Adenosine monophosphate (3'-AMP)3-(2-thienyl)cinnoline-4-carboxylic acidYMH4-ethoxy-6,7-dimethoxyquinazoline2-(2-amino-3-methylbutanamido)-3-phenylpropanoic acidDl-LanthionineTetranor-PGDM(5-L-Glutamyl)-L-Amino AcidINHPhe-PheL-Dihydroorotic Acid5-Methyl-2'-deoxycytidineGly-Tyracridine-9(10H)-thioneDihydrothymineKanosamine2'-O-Methylcytidine1,3-DimethyluracilPalmitic AcidN'2-benzylidene-5-hex-1-ynylfuran-2-carbohydrazideH-Gly-Pro-OH1-(4-benzylpiperazino)-2-(pyridin-2-ylamino)propan-1-oneN2-neopentyl-5-methyl-1,3,4-thiadiazol-2-amineD-(+)-Proline5-fluoro AKB48 N-(4-hydroxypentyl) metaboliteO1-[4-(tert-butyl)benzoyl]-2-(tert-butylsulfonyl)ethanehydroximamideN-Acetyl-L-leucine5-Methoxyindole-3-CarbaldehydeImidazoleacetic acidDL-Glutamine4-(1,2,3-thiadiazol-4-yl)phenyl pivalate2,5-Furandicarboxylic acidPhosphocholinepentane-1,2,3,4,5-pentolXylitolN-[(4-hydroxy-3-methoxyphenyl)methyl]-8-methylnonanamideJWH 250 N-pentanoic acid metaboliteValylprolineWKKN-(4-piperidinophenyl)-2-thiophenecarboxamideEstroneN1-(2-methyl-4-nitrophenyl)acetamidefalseDeletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses_culture supernatantGliotoxin is a secondary metabolite produced by Aspergillus fumigatus, known to play a significant role in immune regulation and virulence phenotypes. This study investigates how the deletion of gliotoxin biosynthesis remodels the metabolic state of A. fumigatus and subsequently affects host immune responses. We employed a multi-omics approach, integrating fungal metabolomics and host transcriptomics data, to assess the impact of gliotoxin deletion on intra- and extracellular metabolic processes. The results showed that the loss of gliotoxin biosynthesis significantly altered the metabolic profile of A. fumigatus, with distinct changes observed in metabolites from both fungal mycelia and culture supernatants. These metabolic alterations were closely linked to changes in host cellular immune-related pathways. Notably, in macrophages and epithelial cells, treatment with gliotoxin led to significant changes in immune gene expression, revealing its role in modulating host immune responses.Furthermore, treating macrophages with gliotoxin inhibited the secretion of inflammatory cytokines and reduced the cells' phagocytic capacity for A. fumigatus conidia, indicating that gliotoxin may play a role in suppressing host defense mechanisms. However, transcriptomic analysis also suggested that exposure to gliotoxin could promote the expression of certain immune genes, pointing to a complex interaction between the fungal metabolite and the host immune system. Through integrative analysis, we identified key metabolic and immune pathways affected by gliotoxin. These findings provide new insights into the impact of gliotoxin deletion on fungal virulence and host immunity, and offer a theoretical basis for potential therapeutic strategies in managing Aspergillus fumigatus infections.2026-05-112026-03-09MTBLS14011Com_12_negCom_29_negCom_62_negCom_79_negCom_87_negCom_122_negCom_135_negCom_181_negCom_190_negCom_219_negCom_325_negCom_427_negCom_428_negCom_475_negCom_604_negCom_615_negCom_648_negCom_657_negCom_678_negCom_714_negCom_735_negCom_748_negCom_814_negCom_845_negCom_887_negCom_930_negCom_933_negCom_955_negCom_1072_negCom_1154_negCom_1259_negCom_1632_negCom_2065_negCom_2148_negCom_2188_negCom_2413_negCom_2845_negCom_3735_negCom_4659_negCom_6936_negCom_78_negCom_172_negCom_242_negCom_150_negCom_314_negCom_223_negCom_525_negCom_1294_negCom_1540_negCom_545_negCom_1198_negCom_363_negCom_4157_negCom_2_negCom_5_negCom_57_negCom_59_negCom_73_negCom_95_negCom_103_negCom_104_negCom_108_negCom_130_negCom_132_negCom_133_negCom_139_negCom_159_negCom_183_negCom_189_negCom_207_negCom_208_negCom_224_negCom_233_negCom_236_negCom_328_negCom_343_negCom_348_negCom_369_negCom_376_negCom_389_negCom_396_negCom_401_negCom_415_negCom_417_negCom_432_negCom_436_negCom_437_negCom_444_negCom_459_negCom_471_negCom_489_negCom_505_negCom_514_negCom_529_negCom_530_negCom_534_negCom_538_negCom_556_negCom_596_negCom_603_negCom_641_negCom_647_negCom_660_negCom_664_negCom_700_negCom_709_negCom_732_negCom_754_negCom_869_negCom_875_negCom_877_negCom_878_negCom_918_negCom_941_negCom_1035_negCom_1039_negCom_1073_negCom_1076_negCom_1100_negCom_1132_negCom_1133_negCom_1144_negCom_1165_negCom_1181_negCom_1224_negCom_1233_negCom_1235_negCom_1275_negCom_1277_negCom_1286_negCom_1287_negCom_1296_negCom_1302_negCom_1327_negCom_1328_negCom_1394_negCom_1459_negCom_1507_negCom_1527_negCom_1547_negCom_1548_negCom_1596_negCom_1626_negCom_1644_negCom_1666_negCom_1682_negCom_1747_negCom_1756_negCom_1764_negCom_1804_negCom_1816_negCom_1860_negCom_1957_negCom_1964_negCom_1971_negCom_1982_negCom_1993_negCom_2045_negCom_2067_negCom_2100_negCom_2124_negCom_2156_negCom_2204_negCom_2215_negCom_2260_negCom_2282_negCom_2286_negCom_2353_negCom_2360_negCom_2382_negCom_2399_negCom_2408_negCom_2411_negCom_2475_negCom_2599_negCom_2620_negCom_2627_negCom_2686_negCom_2690_negCom_2702_negCom_2712_negCom_2738_negCom_2744_negCom_2882_negCom_2909_negCom_3131_negCom_3157_negCom_3246_negCom_3284_negCom_3324_negCom_3346_negCom_3485_negCom_3714_negCom_3809_negCom_3837_negCom_3918_negCom_3927_negCom_3939_negCom_3947_negCom_3982_negCom_4021_negCom_4066_negCom_4114_negCom_4138_negCom_4175_negCom_4227_negCom_4256_negCom_4309_negCom_4404_negCom_4419_negCom_4450_negCom_4485_negCom_4613_negCom_4619_negCom_4782_negCom_4865_negCom_4952_negCom_5032_negCom_5079_negCom_5148_negCom_5180_negCom_5232_negCom_5286_negCom_5315_negCom_5327_negCom_5345_negCom_5352_negCom_5426_negCom_5469_negCom_5660_negCom_5664_negCom_5665_negCom_5701_negCom_5705_negCom_5834_negCom_5837_negCom_6155_negCom_6166_negCom_6230_negCom_6514_negCom_6528_negCom_6549_negCom_6716_negCom_6770_negCom_6828_negCom_6831_negCom_6836_negCom_7048_negCom_7058_negCom_7095_negCom_7165_negCom_7274_negCom_7385_negCom_7438_negCom_7700_negCom_7707_negCom_7739_negCom_7804_negCom_7870_negCom_7967_negCom_7996_negCom_8010_negCom_8129_negCom_8153_negCom_8285_negCom_8329_negCom_8386_negCom_8705_negCom_8762_negCom_8799_negCom_8943_negCom_9073_negCom_9110_negCom_9170_negCom_9395_negCom_9508_negCom_9621_negCom_9729_negCom_9931_negCom_10010_negCom_10044_negCom_10051_negCom_10072_negCom_10472_negCom_10582_negCom_10722_negCom_10885_negCom_11076_negCom_11082_negCom_11156_negCom_11341_negCom_11358_negCom_11423_negCom_11694_negCom_13_negCom_33_negCom_88_negCom_225_negCom_203_negCom_508_negCom_51_negCom_25_negCom_827_negCom_593_negCom_473_negCom_1026_negCom_1162_negCom_94_negCom_1176_negCom_597_negCom_118_negCom_1288_negCom_807_negCom_1110_negCom_1188_negCom_724_negCom_92_negCom_1462_negCom_1573_negCom_701_negCom_1528_negCom_2452_negCom_4172_negCom_157_negCom_212_negCom_527_negCom_1406_negCom_1637_negCom_699_negCom_4343_negCom_2096_negCom_2506_negCom_1476_negCom_4008_negCom_4562_negCom_1324_negCom_8889_negCom_3361_negCom_1045_negCom_3901_negCom_106_negCom_5308_negCom_3972_negCom_5059_negCom_2_posCom_26_posCom_31_posCom_53_posCom_55_posCom_57_posCom_98_posCom_107_posCom_161_posCom_260_posCom_269_posCom_292_posCom_301_posCom_308_posCom_507_posCom_530_posCom_574_posCom_589_posCom_809_posCom_842_posCom_861_posCom_937_posCom_1242_posCom_1251_posCom_1253_posCom_1378_posCom_1582_posCom_2371_posCom_2394_posCom_2639_posCom_2879_posCom_2967_posCom_4718_posCom_4850_posCom_5947_posCom_7191_posCom_16_posCom_17_posCom_20_posCom_35_posCom_93_posCom_125_posCom_217_posCom_248_posCom_251_posCom_575_posCom_594_posCom_606_posCom_672_posCom_821_posCom_1171_posCom_1232_posCom_1238_posCom_2563_posCom_2768_posCom_3350_posCom_6141_posCom_12_posCom_36_posCom_42_posCom_50_posCom_61_posCom_132_posCom_140_posCom_143_posCom_162_posCom_170_posCom_182_posCom_207_posCom_219_posCom_231_posCom_240_posCom_265_posCom_275_posCom_276_posCom_291_posCom_293_posCom_315_posCom_317_posCom_334_posCom_364_posCom_384_posCom_401_posCom_406_posCom_418_posCom_429_po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