And then peakswerematched with the mzCloud
(https://www.mzcloud.org/), mzVault and MassList database to obtain the accuratequalitative and relative quantitative results.Statistical analyses were performed usingthe statistical software R(R version R-3.4.3),Python (Python 2.7.6 version) andCentOS (CentOS release 6.6),When data were not normally distributed, normaltransformations were attempted using of area normalization method
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (Thermo Fisher, Germany) coupled with an Orbitrap Q Exactive™ HF-X mass spectrometer (Thermo Fisher, Germany) at Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypersil Gold column (100 × 2.1 mm, 1.9 µm) using a 17-minute linear gradient at a flow rate of 0.2 mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in water) and eluent B (methanol). The eluents for the negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (methanol). The solvent gradient was set as follows: 2% B, 1.5 min; 2–100% B, 3 min; 100% B, 10 min; 100–2% B, 10.1 min; 2% B, 12 min.
"],"publication":["Deletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses."],"submitter_affiliation":["Capital Medical University"],"submitter_name":["yuqing sun"],"organism_part":["Mycelium"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The samples were placed in the EP tubes and resuspended with prechilled 80%methanol by well vortex. Then the samples were melted on ice and whirled for 30 s.After the sonification for 6 min, they were centrifuged at 5,000 rpm, 4°C for 1 minThe supernatant was freeze-dried and dissolvedwith 10% methanol. Finally, thesolution was injected into the LC-MS/MS system analysis
"],"organism":["Aspergillus fumigatus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14012"],"author":["Yuqing Sun. Chinese Center For Disease Control and Prevention. sunyq0519@163.com."],"data_transformation_protocol":["The raw data files generated by UHPLC-MS/MS were processed using the CompoundDiscoverer 3.1 (CD3.1, ThermoFisher) to perform peak alignment, peak picking, andquantitation for each metabolite. The main parameterswere set as follows: retentiontime tolerance, 0.2 minutes; actual mass tolerance, Sppm; signal intensity tolerance30%; signalnoise ratio, 3; and minimum intensity, et al. After that, peak intensitieswere normalized to the total spectral intensity.The normalized data was used topredict the molecular formula based on additive ions, molecular ion peaks andions.
"],"study_factor":["Treatment"],"submitter_email":["sunyq0519@163.com"],"sample_collection_protocol":["After culturing Aspergillus fumigatus for 24 hours, the culture was centrifuged at 8000 rpm for 10 minutes. The supernatant was discarded, and the pellet was immediately frozen in liquid nitrogen.
"],"omics_type":["Metabolomics"],"study_design":["mycelium","gliotoxin","Aspergillus fumigatus","Immunity","untargeted metabolite profiling"],"curator_keywords":["mycelium","gliotoxin","Aspergillus fumigatus","Immunity","untargeted metabolite profiling"],"mass_spectrometry_protocol":["QExactiveTM HlF-X mass spectrometer was operated in positive/negative polarity modewith spray voltage of 3.5 kV, capillary temperature of 320°C, sheath gas flow rate of35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heatertemperature of350°C
"],"metabolite_name":["3-(4-ethoxyphenyl)-2-(tetrahydro-1H-pyrrol-1-ylcarbonyl)acrylonitrile","N~1~-(2,5-dimethoxyphenyl)-N~2~-(2-pyridin-2-ylethyl)ethanediamide","HNH","HNK","Prostaglandin G2","DL-Panthenol","Monolaurin","2-(benzylamino)-6-methyl-5-(2-methyl-1,3-thiazol-4-yl)nicotinonitrile","13(S)-HOTrE","N-Acetyl-L-histidine","U-47700-d6","Imidazolelactic acid","Stearoyl ethanolamide","Dl-3,4-Dihydroxymandelic Acid","Lysopa 16:0","2-[(3S)-1-(2-Thienylmethyl)-3-pyrrolidinyl]-1,3-benzoxazole","8-Aminooctanoic acid","WLK","ACar 5:0","PC (18:2/18:3)","5-Hydroxymethyl-2-furancarboxylic acid","L-Histidine","Isobutyryl carnitine","(2E)-3-phenyl-N-(2-phenylethyl)prop-2-enamide","Normorphine","2-Arachidonoyl glycerol","4-[4-(1-phenyl-1H-1,2,4-triazol-5-yl)-1,3-thiazol-2-yl]pyridine","L-Tyrosinemethylester","D-Sphingosine","2-(3,4-dihydroxyphenyl)-7-hydroxy-3,4-dihydro-2H-1-benzopyran-4-one","ethyl 2-[3-oxo-5,6-diphenyl-1,2,4-triazin-2(3H)-yl]acetate","2,3-Dinor-TXB2","3-Hydroxyanthranilic Acid","YQH","Tropolone","6-Methoxy-2-naphthoic acid","N-Acetyl-D-tryptophan","HPH","HPK","FLK","7-(2-aminophenyl)heptanoic acid","1-(4-benzoylpiperidino)-2-[(4-chlorophenyl)thio]ethan-1-one","Prostaglandin E2","Meclocycline","Proline-hydroxyproline","Prostaglandin E1","2-(1,3-benzodioxol-5-yl)-7-ethylimidazo[1,2-a]pyridine","N6-Me-dA","20-Carboxy-Leukotriene B4","N6-Acetyl-L-lysine","WNK","5,6-dihydroxyindole","3-(propan-2-yl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione","2-hydroxy-6-[(8Z,11Z)-pentadeca-8,11,14-trien-1-yl]benzoic acid","Boldione","HQH","N-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy)phenyl]urea","N1,N1-dimethyl-4-fluoro-2-nitro-5-tetrahydro-1H-pyrrol-1-ylaniline","Glutaric Acid","Oxohongdenafil","4-(5-propyl-2-pyridyl)benzonitrile","PC (16:1e/2:0)","D-(+)-Maltose","(+/-)12(13)-DiHOME","Fexofenadine","Isophorone","4-Methyl-5-thiazoleethanol","D-(-)-Aspartic acid","1,4-Dihydro-1-Methyl-4-Oxo-3-Pyridinecarboxamide","N-(4-piperidinophenyl)benzamide","Thiazolidine-4-carboxylic acid","N-Acetylneuraminic acid","FNK","Indoxylsulfuric acid","Vindoline","4-(cyclohexylmethyl)-6-(2-thienyl)-2,3-dihydropyridazin-3-one hydrate","3-hydroxy-2-octylpentanedioic acid","Pheniramine N-oxide","5-fluoro AB-PINACA N-(4-hydroxypentyl) metabolite","7-Ketocholesterol","L-Ornithine","PC (17:0/18:2)","5,6-dimethyl-4-oxo-4H-pyran-2-carboxylic acid","4-Morpholinepropanesulfonic acid","5,6-Dihydroxyindole-2-Carboxylic Acid","N-Formylkynurenine","Neosaxitoxin","S-Lactoyglutathione","Valerophenone","Ethyl chrysanthemumate","Oxaceprol","DKK","4-oxododecanedioic acid","Thymopentin","Mupirocin","1-Phenyl-3-methyl-5-pyrazolone","Asp-glu","1-(4-methylphenyl)-3,5-diphenylpent-2-ene-1,5-dione","8Z,11Z,14Z-Eicosatrienoic acid","(12Z)-9,10,11-trihydroxyoctadec-12-enoic acid","5-Henicosyl-1,3-benzenediol","PC (7:0/8:0)","UMP","2-Phenylglycine","Androstenedione","4-(4-hydroxy-3,5-dimethylphenyl)-2-methylphthalazin-1(2H)-one","(2-morpholino-5-nitrophenyl)methanol","FPH","FPK","4,4'-dimethoxy[1,1'-biphenyl]-2-carbonitrile","PC (14:1e/5:0)","DLK","Indole","Octadeca-11E,13E,15Z-trienoic acid","DL-Arginine","10-Nitrolinoleate","4-(2,3-dihydro-1,4-benzodioxin-6-yl)butanoic acid","DL-3,4-Dihydroxyphenyl glycol","L-Carnitine","L-Hydroxylysine","4,6,8-trihydroxy-7-methoxy-3-methyl-3,4-dihydro-1H-2-benzopyran-1-one","FQH","o-Cresol","1-(1,3-benzodioxol-5-yl)-2-[4-(9H-fluoren-9-yl)piperazino]-1-ethanone","DMK","PE (18:2/18:2)","Gly-Tyr-Ala","N-Desmethylclobazam","DL-Norvaline","3,14-dihydro-15-keto-tetranor Prostaglandin E2","2-Hydroxycinnamic acid","23-Norcholic acid","SKK","6-(benzylamino)-3-phenyl-1,4-dihydropyridazin-4-one","2-(14,15-Epoxyeicosatrienoyl) glycerol","D-Erythrose 4-phosphate","3,4,5-trihydroxy-6-methyloxan-2-yl 2-(methylamino)benzoate","2-[5-(2-hydroxypropyl)oxolan-2-yl]propanoic acid","FRH","DNK","2-Deoxyuridine","Norfludiazepam","PC (14:1e/3:0)","L-Phenylalanine","3-[1-(dimethylamino)-1H-pyrrol-2-yl]-2-(2-furylcarbonyl)acrylonitrile","5-Methylcytosine","Ala-Leu","Docosahexaenoic acid","Thioguanine","Isoproterenol","SLH","2,5-bis(4-hydroxy-3-methoxyphenyl)-3,4-dimethyloxolan-3-ol","Oleanolic acid","LPE 20:1","LPE 20:2","6 ¦Â-Hydroxycortisol","(5-hydroxy-3-methyl-1-phenyl-1H-pyrazol-4-yl)(phenyl)methanone","N'3-(3,4,5-trimethoxybenzylidene)pyridine-3-carbohydrazide","SLK","tert-Butyl N-[1-(aminocarbonyl)-3-methylbutyl]carbamate","(+)-Evodiamine","gamma-Glutamylleucine","gamma-Glutamylmethionine","Tetrahydrocortisone","Celestolide","2-(cyclopropylimino)-3-(2-thienylmethyl)-1,3-thiazolan-4-one","Nitrazepam-d5","SMH","SMK","6-Sialyllactose","12,13-EODE","DPH","DPK","DL-Tryptophan","4-(3-Methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acid","cis-7-Hexadecenoic Acid","6-benzyl-4-oxo-1,4-dihydropyridine-3-carboxamide","2,3,4-Trihydroxybenzoic acid","SNH","L-Glutamic acid monosodium salt","PC (16:2e/4:0)","Guanine","2-Naphthol","SNK","N-Acetyl-L-carnosine","Adenosine 5'-Diphosphate","Penta-N-acetylchitopentaose","(3S,9aS)-3-benzyl-octahydro-1H-pyrido[1,2-a]pyrazin-1-one","Lysopg 18:1","Uridine 5'-monophosphate","1,4-dihydroxyheptadec-16-en-2-yl acetate","QKK","7-Methylguanine","Bicyclo prostaglandin E2","6-(3-hydroxybutan-2-yl)-5-(hydroxymethyl)-4-methoxy-2H-pyran-2-one","N-{[(2R,4S,5R)-5-Ethyl-1-azabicyclo[2.2.2]oct-2-yl]methyl}-2-furamide","3-(4-hydroxy-3-methoxyphenyl)propanoic acid","(3beta,9xi)-3-(beta-D-Glucopyranosyloxy)-14-hydroxycard-20(22)-enolide","DRH","Prostaglandin K2","11-Epiprostaglandin E1","MJN110","1-(7-methoxy-2-oxo-2H-chromen-8-yl)-3-methyl-2-oxobutyl acetate","SPH","QLH","4-[4-(trifluoromethoxy)anilino]-2H-1,3-benzoxazin-2-one","Adenosine 3'5'-cyclic monophosphate","Pipecolic acid","SPK","Indole-3-acrylic acid","QLK","4-(4-methoxyphenyl)-2-(2-thienyl)pyrimidine","2'-Deoxyuridine","15-epi Prostaglandin A1","Cinnamoylglycine","4-(3,4-dihydro-2H-1,5-benzodioxepin-7-ylamino)-4-oxobutanoic acid","Bisphenol G","QMK","(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-(2-phenylethoxy)oxane-3,4,5-triol","6-cyclohexyl-5-(cyclohexylimino)-4-thia-6-azaspiro[2.4]heptan-7-one","2-{[(4,5-dimethoxy-2-nitrophenethyl)imino]methyl}phenol","3-hydroxy-4-(3-hydroxyphenyl)-1,2-dihydroquinolin-2-one","Ofloxacin impurity E","Taurochenodeoxycholic acid","2'-Deoxyadenosine-5'-monophosphate","Ecgonine methyl ester","2-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]pyridin-3-amine","5-Hydroxymebendazole","N3,N4-Dimethyl-L-arginine","THJ2201 N-(5-hydroxypentyl) metabolite","Cafestol","J147","2-Benzyl-5-[(3S)-1-isopropyl-3-pyrrolidinyl]-1,3,4-oxadiazole","QNK","N1-{4-methyl-2-[(3,4,5-trimethoxybenzylidene)amino]phenyl}acetamide","cis-Aconitic acid","N epsilon,N epsilon,N epsilon-trimethyllysine","(2E,4E)-N-[2-(4-hydroxyphenyl)ethyl]dodeca-2,4-dienamide","Prostaglandin J2","Thymine","9,10-Dihome","PC (14:0e/3:0)","Physostigmine","2-[(3S)-1-(2-Pyridinylmethyl)-3-pyrrolidinyl]-1,3-benzothiazole","Stercobilin","Oleoyl-L-alpha-lysophosphatidic acid","1-Methyladenosine","N-Acetylglucosamine 1-phosphate","4-Hydroxyisoleucine","L-Cysteine-glutathione gisulfide","1-Methylhistamine","Coenzyme Q2","Gamma-Glu-Leu","QPH","4-(4-methoxyphenyl)-6-pyridin-4-yl-1,3,5-triazin-2(3H)-one","3-Hydroxy-3-methylbutanoic acid","Lithocholic acid","2-({2-[(3-methyl-5-cinnolinyl)amino]-2-oxoethyl}sulfanyl)acetic acid","Pro-Leu","5-methoxy-8,8-dimethyl-2-phenyl-4H,8H-pyrano[2,3-h]chromen-4-one","N-Acetylsphingosine","L-Threonine","Guanosine 3',5'-cyclic monophosphate","o-Toluic Acid","Oxolamine","D-Alanyl-D-alanine","LDGTS 16:0","Andrographolide","3-(5-phenyl-1,3-oxazol-2-yl)-4-(trifluoromethyl)pyridine","LSD-d3","5-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-2-one","Pyridoxal","N-Acetyl-Asp-Glu","5,8-dihydroxy-10-methyl-5,8,9,10-tetrahydro-2H-oxecin-2-one","(+/-)11(12)-EET","(1E)-5-hydroxy-1,7-diphenylhept-1-en-3-one","N-Propionylglycine","N6-Succinyl Adenosine","diethyl 2-[(4-benzhydrylpiperidino)methylidene]malonate","11-Dehydro thromboxane B2","Lysopc 16:1","R-1 Methanandamide phosphate","MF498","2,3-dihydroxypropyl 12-methyltridecanoate","L-Palmitoylcarnitine","L-Tryptophan","Orlistat","PC (16:0e/3:0)","13,14-Dihydro-15-keto Prostaglandin A2","N-Tetradecanamide","4-Guanidinobutanoic acid","gamma-Tocopherol","Glycitin","L-Leucyl-L-alanine Hydrate","W-15-d4","Testosterone","O-Desmethylnaproxen","N1-[4-hydroxy-6-(methoxymethyl)pyrimidin-2-yl]acetamide","Eicosapentaenoic acid","Uracil","Cuminaldehyde","L-Kynurenine","(2,6-dimethylmorpholino)(1-methyl-5-nitro-1H-pyrazol-4-yl)methanone","15-Acetyldeoxynivalenol","1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene acetaldehyde","Desmethylclozapine","2-[2-(2,6-Dimethylmorpholino)-2-oxoethoxy]phenyl N,N-dimethylcarbamate","16-Heptadecyne-1,2,4-triol","Cytosine","Ciprostene","LPC 16:2","LPC 16:0","11-Deoxy prostaglandin F1¦Á","MMK","N-Acetyl-5-aminosalicylic acid","N-acetyl-D-glucosamine","Prostaglandin E2-1-glyceryl ester","N-acetyl-L-ornithine","PC (17:1/17:2)","LPE 19:1","N1-cyclooctyl-4-hydroxy-1-piperidinecarbothioamide","PE (17:1/17:2)","N-(4-fluorophenyl)-N'-(2-piperidinophenyl)urea","MNK","2,6-Dihydroxypurine","Nicotinuric Acid","Flurazepam-d10","4-Hydroxytamoxifen","L-Glutamic acid","3-(methylsulfonyl)-2H-chromen-2-one","KKK","4-oxo-4-[(1-phenylethyl)amino]but-2-enoic acid","dAMP","2-(2-thienyl)-4H-chromen-4-one","Ergosta-5,7,9(11),22-Tetraen-3-beta-Ol","dopaquinone","Palmitoleic acid","3-benzyl-1-methyl-2-oxo-1,2-dihydroquinolin-4-yl benzoate","Citraconic acid","(11E,15Z)-9,10,13-trihydroxyoctadeca-11,15-dienoic acid","Taurocholic acid sodium salt hydrate","L-(+)-Citrulline","MPH","Desthiobiotin","LysoPC 20:2","Dl-3-Hydroxy-kynurenine","MPK","3-[(4-chlorobenzyl)thio]-4-methyl-5-undecyl-4H-1,2,4-triazole","3-(3,4-dimethoxyphenyl)-1-(2-hydroxy-4,6-dimethoxyphenyl)propan-1-one","D-Panthenol","L-beta-Imidazolelactic acid","GDP","16-Hydroxyhexadecanoic acid","UR-144 N-(2-hydroxypentyl) metabolite","Stearamide","Tyramine","4,7-diphenyl[1,10]phenanthroline","CYM-5442","(6E)-7-(2H-1,3-benzodioxol-5-yl)-1-(piperidin-1-yl)hept-6-en-1-one","Nervonic acid","(¡À)8-HEPE","3b,7b-Dihydroxy-5-androsten-17-one","Sphingosine (d18:1)","KMH","MGMG (18:2)","(-)-Camphor","Sedanolide","Gly-Val","Deoxyadenosine","5,6-dimethoxy-2-(2-methoxyphenyl)-4H-chromen-4-one","N-(4-methoxyphenethyl)-N'-(2-methylphenyl)thiourea","Gatifloxacin","Asp-Phe methyl ester","LPE 17:2","N4-Acetylcytidine","S-Adenosyl-L-methionine","LPE 17:0","Azetidine-2-carboxylic acid","Cyclohexylsulfamate","KNH","Royal jelly acid","KNK","3-(benzylthio)-5-[4-(tert-butyl)phenyl]-4-methyl-4H-1,2,4-triazole","Thr-Leu","Choline Glycerophosphate","2-Aminobenzenesulfonic acid","Xanthurenic Acid","Cystamine","All-Trans-13,14-Dihydroretinol","Dl-Threitol","Carbaprostacyclin","IKK","Xanthine","Diflorasone","3-(3,4-Dihydroxyphenyl)-2-Methylalanine","Gemifloxacin","L-Glutamine","D-Glucosamine","Choline","DL-Leucineamide","Dinophysistoxin-1","KPH","N-Butylbenzenesulfonamide","Octadecanamine","(5E)-7-methylidene-10-oxo-4-(propan-2-yl)undec-5-enoic acid","Monocrotaline","KPK","Acetylcysteine","Methyl N-benzoylphenylalaninate","5-Hydroxymethyluracil","L-Alanyl-L-proline","PC (16:2e/19:2)","TBB","4-(Diethylamino)salicylaldehyde","Inosine 5'-Monophosphate","Carvone","KQH","N-Acetyl-D-lactosamine","AKB48 N-(4-hydroxypentyl) metabolite","3-Amino-4-methylpentanoic acid","IMK","3-Methoxycinnamic acid","Homo-Gamma-Linolenic Acid (C20:3)","3-Methylindole","2-Amino-1,3-octadecanediol","S-Adenosylhomocysteine","cyclohexyl{4-[4-nitro-2-(1H-pyrrol-1-yl)phenyl]piperazino}methanone","Quinidine","Diosgenin","Lysopc 18:3","LPE 15:0","3'-Hydroxystanozolol","INK","4-methyl-3-(methylthio)-5-undecyl-4H-1,2,4-triazole","1,7,8-trihydroxy-3-methyl-1,2,3,4,7,12-hexahydrotetraphen-12-one","Thromboxane B2","L-Alanyl-L-Lysine","5-Methyl-2'-deoxycytidine","16¦Á-Hydroxydehydroepiandrosterone","3-(3-morpholinopropyl)-2-(2-pyridinyl)-2,3-dihydro-4(1H)-quinazolinone","2-(Dimethylamino)Guanosine","Kanosamine","2-[(3S)-1-(3,4-Difluorobenzyl)-3-pyrrolidinyl]-1,3-benzothiazole","3-hydroxy-2-(3-nitro-4-piperidinobenzyl)propanenitrile","H-Gly-Pro-OH","D-(+)-Proline","Feruloylcholine","GKK","3-hydroxy-2-[5-nitro-2-(1-pyrrolidinyl)benzyl]propanenitrile","DL-Glutamine","p-Coumaric acid","3-Acetoxyurs-12-en-23-oic acid","Glycochenodeoxycholic acid sodium salt","1-(3,4-dimethylphenyl)-3-piperidinopyrrolidine-2,5-dione","Phosphocholine","pentane-1,2,3,4,5-pentol","5-Methyl-dl-tryptophan","Lysopc 16:2 (2N Isomer)","Xylitol","N-[(4-hydroxy-3-methoxyphenyl)methyl]-8-methylnonanamide","1,2,10-Trimethoxy-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-9-ol","(¡À)9-HpODE","Estrone","D-2-Aminoadipic acid","2-Methoxyestradiol (2-MeOE2)","alpha-Farnesene","IPK","1-morpholino-3-(4-nitrophenoxy)propan-2-ol","GLK","Riboflavin","L-(-)-alpha-Amino-epsilon-Caprolactam","Deoxyinosine","5¦Á-Dihydrotestosterone","Feruloyl Putrescine","PC (22:4e/18:5)","2-mercapto-4,6-dimethylnicotinonitrile","PC (14:1e/6:0)","Milbemycin A4 oxime","Lenalidomide","(+)-ar-Turmerone","Biotin","Flunitrazepam-d3","N2-Methylguanosine","2-Methylbutyroylcarnitine","Methyl beta-D-galactopyranoside","Benzamidine Hydrochloride Hydrate","IRH","1-(2-methoxy-4-nitrophenyl)pyrrolidine","4,7,8-trimethoxyfuro[2,3-b]quinoline","GNK","N-(1-adamantyl)-N'-(2-chlorobenzyl)thiourea","HexCer-NS (d18:2/18:2)","PC (14:0e/2:0)","THC","CHEMBL1616521","cis-4-Hydroxy-D-proline","VLH","JWH 073 N-(4-hydroxybutyl)-d5","Guanosine monophosphate","(+/-)11(12)-DiHET","VLK","2-[(3S)-1-(2-Chlorobenzyl)-3-pyrrolidinyl]-5-methyl-1,3,4-oxadiazole","Oleic acid","Estriol","2-[(3S)-1-Benzyl-3-pyrrolidinyl]-1,3-benzothiazole","2-[(5-anilino-4-phenyl-4H-1,2,4-triazol-3-yl)thio]acetic acid","Lysopc 20:4","EKK","Cytidine 5'-monophosphate (hydrate)","5-S-cysteinyldopa","3-Anilinoacraldehyde anil, HCl salt","Amitriptyline-d3","11-Oxoetiocholanolone","6-Phosphogluconic Acid Trisodium Salt","VMK","N-benzyl-2-(6-methoxy-2-naphthyl)propanamide","1,2-di[3-(benzyloxy)benzylidene]hydrazine","(+)-alpha-Lipoic acid","LPE 14:0","GPH","4-Pyridoxic acid","Octyl hydrogen phthalate","5-[4-(phenylsulfonyl)phenyl]-1H-pyrazole","2,4-dihydroxyheptadec-16-en-1-yl acetate","glutathione disulfide","GPK","Tyrosylalanine","ELK","N-Acetyl-L-methionine","Adenosine diphosphate (ADP)","4-{4-[3-(4-chlorophenoxy)propyl]piperazino}-1H-indole","5'-S-Methyl-5'-thioadenosine","DL-Lysine","VNK","(1E,4E)-1,5-bis(4-methoxyphenyl)penta-1,4-dien-3-one","dUDP","¦Á-Linolenoyl ethanolamide","Lysope 16:0","1,7-bis(4-hydroxyphenyl)-5-methoxyheptan-3-one","7-(3,4-dihydroxyphenyl)-5-hydroxy-1-(4-hydroxyphenyl)heptan-3-one","Boc-beta-cyano-L-alanine","2-furyl[4-(1H-indol-4-yl)piperazino]methanone","GQH","EMK","17¦Á-Hydroxyprogesterone","3,4-dihydroxy-4-(4-methoxyphenyl)-1,2,3,4-tetrahydroquinolin-2-one","L-Fucose","2-(4,4-diphenyl-1-piperidinobuta-1,3-dienyl)phenyl acetate","Alanyltyrosine","2-(1-benzofuran-2-yl)-4H-chromen-4-one","TKK","1-Methylguanine","GRH","Fmoc-L-Isoleucine","D-Gluconic acid","2,4-dichloro-N-{2-[(2-furylmethyl)amino]-1-methyl-2-oxoethyl}benzamide","Ocfentanil-d5","ENK","Dulcitol","Proline","cGMP","Angiotensin IV","U-44069","¦´-L-Glutamyl-L-glutamic acid","2-[(3S)-1-(3,4-Difluorobenzyl)-3-pyrrolidinyl]-1,3-benzoxazole","VPH","Puromycin","TLH","TLK","Fasciculic acid B","Thiamine","1,2-dihydroxyheptadec-16-yn-4-yl acetate","VQH","TMK","Fumaric acid","L-Tyrosine","Argininosuccinic acid","Cyclohexaneacetic acid","(-)-Caryophyllene oxide","EPK","PC (19:2/18:5)","Phe-Pro","3-hydroxy-3-methylpentanedioic acid","5-Phenylvaleric Acid","gamma-Glutamyltyrosine","Glycylproline","N-(5-acetamidopentyl)acetamide","TNK","L-Canavanine","5-(2,4-difluorophenyl)-2-furaldehyde oxime","Norverapamil","methyl 3,4,5-trihydroxycyclohex-1-ene-1-carboxylate","3-(4-methylpiperazino)-5,6-diphenyl-1,2,4-triazine","Spermidine","S-(Methyl)Glutathione","2-amino-8-mercapto-9H-purin-6-ol","LPC 18:3","EQH","farensyl diphosphate","Ergosterol peroxide","2-(tert-butyl)-1,3-thiazolane-4-carboxylic acid","4-phenoxyphenyl 4-hydroxypiperidine-1-carboxylate","Spectinomycin","Meperidine-d5","Rauwolscine","Avocadyne 1-acetate","Hydroxyglutaric acid","Propionylcarnitine","RKK","4-decyl-3-hydroxy-5-oxooxolane-2,3-dicarboxylic acid","ERH","Ureidoisobutyric Acid","p-Mentha-1,3,8-triene","3-amino-2-phenyl-2H-pyrazolo[4,3-c]pyridine-4,6-diol","3-Acetyl-11-keto-¦Â-boswellic acid","4-methoxy-6-[2-(4-methoxyphenyl)ethyl]-2H-pyran-2-one","2-(benzylthio)-1-phenethyl-5-(trifluoromethyl)-1H-benzo[d]imidazole","JNJ-1661010","N-Acetyl-¦Á-D-glucosamine","5-(tert-butyl)-2-methyl-N-(4-nitrophenyl)-3-furamide","Pyridoxal 5'-phosphate","TPH","RLH","RLK","2,6-Dihydroxybenzoic acid","Acetophenone","2-[2-oxo-2-(2-pyridylamino)ethoxy]acetic acid","N-Acetyl-aspartic acid","Octhilinone","dimethyl 2-(3-nitro-2-pyridyl)malonate","PC (17:2/22:6)","Coniferin","Acetyl-L-carnitine","TQH","Pyridoxine","RMH","RMK","3-(4-morpholinophenyl)-2-(4-nitrophenyl)acrylonitrile","2-Benzyl-5-[(3S)-1-cyclobutyl-3-pyrrolidinyl]-1,3,4-oxadiazole","Glycohyocholic acid Sodium salt","2-Amino-1,3,4-octadecanetriol","ALK","Stiripentol","N-[2-chloro-6-(trifluoromethoxy)phenyl]-2,2-dimethylpropanamide","Linoleoyl ethanolamide","LDGTS 18:2","Bilirubin","9-Oxo-10(E),12(E)-octadecadienoic acid","RNH","RNK","Tenofovir","1-O-(3,4,5-Trimethoxybenzoyl)-beta-L-galactopyranose","mesityl(piperidin-4-yl)methanone hydrochloride","Betaine","DGMG (18:2)","Levodopa","AMK","AL 8810 Methyl ester","4-Acetamidobutanoic acid","6-Acetylmorphine","Cytidine","Enrofloxacin","6-Methylflavone","2-amino-7,9-dimethyl-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrile","Isoquinoline","octadec-9-ynoic acid","2-(4-aminophenoxy)isophthalonitrile","Pyridostigmine","P-Aminohippuric Acid","ANH","ANK","N-Benzylformamide","Cys-Gly","NAT13-331713_POS","4-{2-[(2-chlorophenoxy)methyl]-1,3-thiazol-4-yl}benzonitrile","¦Ø-Hydroxynorfentanyl","7-methyl-3-nitroimidazo[1,2-a]pyridine","Bz-RS-ISer(3-Ph)-Ome","1-Methylhistidine","PLH","5-(2,5-dihydroxyhexyl)oxolan-2-one","Deoxycorticosterone 21-glucoside","PC (14:1e/2:0)","N-{4-[(2R,3R)-3-(Hydroxymethyl)-5-oxo-2-morpholinyl]phenyl}acetamide","2-Thio-acetyl MAGE","¦Á-Lapachone","4-acetyl-4-(ethoxycarbonyl)heptanedioic acid","N'-[6-(tert-butyl)thieno[3,2-d]pyrimidin-4-yl]-4-methylbenzohydrazide","DL-2-(acetylamino)-3-phenylpropanoic acid","RQH","5'-Deoxy-5'-(Methylthio)Adenosine","1-Palmitoylglycerol","PMK","Monoolein","cyclohexyl[4-(2-methoxyphenyl)piperidino]methanone","APH","dUMP","APK","Anthranilic acid","3-(3,4-dihydroxyphenyl)propanoic acid","Prolylleucine","Allantoin","N1,N3-di(2-methylphenyl)-2-phenylmalonamide","2-furaldehyde N-(1-phenyl-1H-pyrazolo[3,4-d]pyrimidin-4-yl)hydrazone","PNK","Arachidonoyl serinol","5-(6-hydroxy-6-methyloctyl)-2,5-dihydrofuran-2-one","5-Hydroxytryptophan","9-KODE","Atropine","L-Saccharopine","Noradrenaline","3-Indoleacrylic acid","LysoPC 12:1","Methylmalonate","Cytidine 5'-diphosphocholine","Leukotriene B4 Ethanolamide","L-Ergothioneine","Muscone","3-Methoxy prostaglandin F1¦Á","Taurochenodeoxycholic Acid (sodium salt)","Methionine","Muramic acid","Phosphopyruvic acid","N-lactoyl-phenylalanine","MAG (18:3)","NKK","1-[(3S)-3-(1,3-Benzoxazol-2-yl)-1-pyrrolidinyl]-3-methoxy-1-propanone","Styrene","ARH","4-oxo-4-(5,6,7,8-tetrahydronaphthalen-1-ylamino)but-2-enoic acid","1H-indol-3-yl(pyridin-2-yl)methanol","PC (16:0e/2:0)","N-gamma-Acetyl-N-2-Formyl-5-Methoxykynurenamine","D-threo-Isocitric acid","L-Histidinol","2,6-Di-tert-butyl-1,4-benzoquinone","Carnosine","PPK","L-Glutathione oxidized","1-Stearoylglycerol","trans-4-Hydroxy-L-proline","4-Butylresorcinol","3-Methyladipic acid","Uridine 5'-Diphospho-N-Acetylgalactosamine","N-allyl-2-(2-methyl-1-benzofuran-3-yl)acetamide","Glu-Gln","LPC 15:0","Diaminopimelic acid","N,N-Diethyldodecanamide","PQH","N-Stearoyl taurine","trans-4-Phenyl-4-piperidinocyclohexanol","NMK","N2-(2,4-dimethylphenyl)-1,3-benzothiazol-2-amine","JWH-018 N-(3-methylbutyl) isomer","Leucylproline","AKB48 N-(4-fluorobenzyl) analog","Gamma-Aminobutyric acid","Nor-9-carboxy-¦Ä9-THC","Ala-Ile","PC (16:2e/2:0)","PC (20:5e/17:2)","NNK","Phytosphingosine","6,15-diketo-13,14-dihydro Prostaglandin F1¦Á","3-(1-naphthylmethylidene)indolin-2-one","UR-144 N-(5-Chloropentyl) analog","Fisetin","(¡À)5(6)-DiHETE","LysoPC 10:0","DL-Carnitine","Homoarginine","Ergocalciferol","Quinine","LKK","Coumarin","N-Desmethylclomipramine","7-(2-hydroxypropan-2-yl)-1,4a-dimethyl-decahydronaphthalen-1-ol","PDMP","Nicotinate ribonucleoside","Spermine","1-(4-Methoxyphenyl)-2-propanone","Hypoxanthine","4-(2-((4-Methoxyphenyl)amino)oxazol-5-yl)benzonitrile","Palmitoyl ethanolamide","Homoserine","2-Phenylpropionic acid","N-Acetyl-DL-phenylalanine","morpholine-4-carboximidamide hydrobromide","Hippuric acid","Heptadecanoic Acid","NPK","LLK","15-Deoxy-¦¤12,14-prostaglandin J2-2-glycerol ester","8-Isoprostaglandin F1¦Á","Shikonin","8-Isoprostaglandin F1¦Â","N-glycyl-L-proline","Methyl palmitate","N6,N6,N6-Trimethyl-L-lysine","5-Hydroxyindole","5-Methyluridine","LPC 17:2","LMH","Adenine","(5-methyl-3-isoxazolyl)[4-(5-propyl-2-pyrimidinyl)piperazino]methanone","Glu-Val-Phe","3-Nitro-L-Tyrosine","D-Proline","(R)-Prunasin","N1-(1H-indol-4-yl)cyclohexane-1-carboxamide","Inosine","Dl-3-Hydroxynorvaline","Naratriptan","Glycerophospho-N-palmitoyl ethanolamine","LNH","LNK","Tetranor-12(S)-HETE","5-[4-(tert-butyl)phenyl]-1,3,4-oxadiazole-2-thiol","Vitamin B12","Arachidonic acid","1,7-bis(4-hydroxyphenyl)heptan-3-one","N-Oleoyl Glycine","Pantethine","Phenylpropiolic acid","(2R)-2-[(2R,5S)-5-[(2S)-2-hydroxybutyl]oxolan-2-yl]propanoic acid","Ala-Gln","2-(cyclopropylcarbonyl)-3-(4-fluoroanilino)acrylonitrile","PB-22 N-(4-Hydroxypentyl)-3-carboxyindole metabolite","2-[1-(4-isobutylphenyl)ethyl]-5-(3-nitrophenyl)-1,3,4-oxadiazole","gamma-Glutamylcysteine","6-Ketoprostaglandin F1¦Á","6-phenyl-1,2,3,4-tetrahydro-2,5-benzodiazocin-1-one","N-Phenylacetylglutamine","N-Acetylhistamine","Nicotinamide","11(Z),14(Z),17(Z)-Eicosatrienoic acid","Methyl dihydrojasmonate","3-Methyl-2-oxobutanoic acid","13-HPODE","Estradiol Benzoate","LPH","7-Ketodeoxycholic acid","H-gamma-glutamyl-glutamine","6-Hydroxymelatonin","Oleoyl ethanolamide","N-(4-butyl-2-methylphenyl)-N'-[4-(4-methylpiperazino)phenyl]urea","D-Glucarate","N7-Methylguanosine","Pyroglutamic acid","Pyridoxamine","4-methoxyphenyl 3,5-dimethyl-1-phenyl-1H-pyrazole-4-carboxylate","Phenothiazine","L-Pyroglutamic acid","9-Oxo-ODE","LQH","Pyridoxal 5-phosphate monohydrate","PC (5:0/13:1)","Androsterone","¦Â-Nicotinamide mononucleotide","PC (17:0/18:3)","3-[(4-hydroxyphenyl)methyl]-octahydropyrrolo[1,2-a]pyrazine-1,4-dione","UR-144 N-(5-Methylhexyl) analog","LPE 18:0","2-methoxy-4-{3-[(4-methylbenzoyl)amino]butyl}phenyl 4-methylbenzoate","LPE 18:2","Beta-Muricholic acid","Acetylcholine","LPE 18:3","5-OxoETE","N1-perhydrocyclopenta[d]pyrimidin-2-ylidenbenzene-1-sulfonamide","1-[4-(1-adamantyl)phenoxy]-3-piperidinopropan-2-ol hydrochloride","N1-(3,4-dimethoxyphenethyl)-4-methylbenzene-1-sulfonamide","Diphenylamine","Pantetheine","Val-Ser","hydroxyphenylpyruvate","6-phenyl-3,4-dihydro-1H-2,5-benzoxazocin-1-one","YLH","Lysopa 18:0","YLK","Nicotinamide N-oxide","UDP","2-[(3-methyl-1-phenyl-1H-pyrazol-5-yl)amino]benzoic acid","HKK","7¦Á-Hydroxytestosterone","lipoamide","Linolelaidic Acid (C18:2N6T)","3'-Adenosine monophosphate (3'-AMP)","N-[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]benzamide","2,6-di(tert-butyl)-4-[2-(4-chlorophenyl)-1,3-thiazol-4-yl]phenol","Nicotinic Acid","4-(methylthio)-6-phenyl-2-(3-pyridyl)pyrimidine-5-carbonitrile","trans-Cinnamaldehyde","Noroxymorphone","cis-gondoic acid","13,14-dihydro-15-keto-tetranor Prostaglandin E2","(5-L-Glutamyl)-L-Amino Acid","Phe-Phe","N-Desmethyl sildenafil","Mag (18:1)","Jervine","Methionine sulfoxide","Zaleplon-d4","HLK","Desalkylflurazepam-d4","Hexadecanamide","Gly-Tyr","acridine-9(10H)-thione","Tauro-alpha-Muricholic acid sodium salt","3-morpholino-5,6-diphenylpyridazine-4-carbonitrile","YNK","1,3-Dimethyluracil","HexCer-NS (d19:2/18:1)","4-[3-(4-phenoxyphenyl)-1H-pyrazol-5-yl]morpholine","4-[1-(acetyloxy)prop-2-en-1-yl]-2-methoxyphenyl 2-methylpropanoate","16(R)-HETE","3-N-Methyl-L-histidine","N-Acetyl-L-leucine","Imidazoleacetic acid","2,5-Furandicarboxylic acid","5beta-Androstane-3,17-dione","2'-Deoxyguanosine","Valylproline","WKK","(S)-2-Hydroxybutanoicacid","Gamithromycin","LPE 16:1"],"additional_accession":[]},"is_claimable":false,"name":"Deletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses_Mycelium","description":"Gliotoxin is a secondary metabolite produced by Aspergillus fumigatus, known to play a significant role in immune regulation and virulence phenotypes. This study investigates how the deletion of gliotoxin biosynthesis remodels the metabolic state of A. fumigatus and subsequently affects host immune responses. We employed a multi-omics approach, integrating fungal metabolomics and host transcriptomics data, to assess the impact of gliotoxin deletion on intra- and extracellular metabolic processes. The results showed that the loss of gliotoxin biosynthesis significantly altered the metabolic profile of A. fumigatus, with distinct changes observed in metabolites from both fungal mycelia and culture supernatants. These metabolic alterations were closely linked to changes in host cellular immune-related pathways. Notably, in macrophages and epithelial cells, treatment with gliotoxin led to significant changes in immune gene expression, revealing its role in modulating host immune responses.
Furthermore, treating macrophages with gliotoxin inhibited the secretion of inflammatory cytokines and reduced the cells' phagocytic capacity for A. fumigatus conidia, indicating that gliotoxin may play a role in suppressing host defense mechanisms. However, transcriptomic analysis also suggested that exposure to gliotoxin could promote the expression of certain immune genes, pointing to a complex interaction between the fungal metabolite and the host immune system. Through integrative analysis, we identified key metabolic and immune pathways affected by gliotoxin. These findings provide new insights into the impact of gliotoxin deletion on fungal virulence and host immunity, and offer a theoretical basis for potential therapeutic strategies in managing Aspergillus fumigatus infections.
","dates":{"publication":"2026-05-11","submission":"2026-03-09"},"accession":"MTBLS14012","cross_references":{"KEGG":["Com_3_neg","Com_13_neg","Com_23_neg","Com_32_neg","Com_39_neg","Com_40_neg","Com_42_neg","Com_44_neg","Com_59_neg","Com_81_neg","Com_93_neg","Com_110_neg","Com_157_neg","Com_267_neg","Com_286_neg","Com_316_neg","Com_333_neg","Com_348_neg","Com_363_neg","Com_366_neg","Com_397_neg","Com_436_neg","Com_489_neg","Com_515_neg","Com_555_neg","Com_734_neg","Com_1021_neg","Com_1354_neg","Com_1481_neg","Com_1502_neg","Com_1516_neg","Com_1721_neg","Com_1866_neg","Com_1878_neg","Com_2835_neg","Com_2862_neg","Com_3018_neg","Com_3599_neg","Com_4445_neg","Com_4934_neg","Com_5766_neg","Com_8509_neg","Com_72_neg","Com_46_neg","Com_268_neg","Com_153_neg","Com_63_neg","Com_149_neg","Com_3990_neg","Com_119_neg","Com_523_neg","Com_266_neg","Com_99_neg","Com_520_neg","Com_773_neg","Com_78_neg","Com_4844_neg","Com_3313_neg","Com_8490_neg","Com_2795_neg","Com_974_neg","Com_2139_neg","Com_2762_neg","Com_452_neg","Com_5_neg","Com_7_neg","Com_20_neg","Com_24_neg","Com_31_neg","Com_41_neg","Com_43_neg","Com_65_neg","Com_86_neg","Com_89_neg","Com_94_neg","Com_97_neg","Com_127_neg","Com_137_neg","Com_148_neg","Com_166_neg","Com_185_neg","Com_205_neg","Com_207_neg","Com_211_neg","Com_236_neg","Com_249_neg","Com_253_neg","Com_275_neg","Com_281_neg","Com_282_neg","Com_297_neg","Com_302_neg","Com_325_neg","Com_354_neg","Com_360_neg","Com_392_neg","Com_404_neg","Com_411_neg","Com_461_neg","Com_476_neg","Com_479_neg","Com_485_neg","Com_511_neg","Com_524_neg","Com_540_neg","Com_542_neg","Com_590_neg","Com_592_neg","Com_657_neg","Com_675_neg","Com_682_neg","Com_692_neg","Com_712_neg","Com_715_neg","Com_722_neg","Com_735_neg","Com_746_neg","Com_772_neg","Com_774_neg","Com_776_neg","Com_789_neg","Com_805_neg","Com_846_neg","Com_911_neg","Com_918_neg","Com_946_neg","Com_969_neg","Com_972_neg","Com_977_neg","Com_981_neg","Com_986_neg","Com_993_neg","Com_994_neg","Com_1056_neg","Com_1065_neg","Com_1066_neg","Com_1090_neg","Com_1099_neg","Com_1119_neg","Com_1121_neg","Com_1135_neg","Com_1137_neg","Com_1149_neg","Com_1164_neg","Com_1181_neg","Com_1195_neg","Com_1241_neg","Com_1254_neg","Com_1291_neg","Com_1292_neg","Com_1337_neg","Com_1377_neg","Com_1386_neg","Com_1388_neg","Com_1406_neg","Com_1418_neg","Com_1419_neg","Com_1459_neg","Com_1504_neg","Com_1526_neg","Com_1539_neg","Com_1571_neg","Com_1629_neg","Com_1630_neg","Com_1635_neg","Com_1650_neg","Com_1711_neg","Com_1712_neg","Com_1739_neg","Com_1746_neg","Com_1770_neg","Com_1802_neg","Com_1820_neg","Com_1833_neg","Com_1891_neg","Com_1911_neg","Com_1939_neg","Com_1984_neg","Com_1991_neg","Com_2000_neg","Com_2009_neg","Com_2010_neg","Com_2064_neg","Com_2092_neg","Com_2104_neg","Com_2138_neg","Com_2162_neg","Com_2169_neg","Com_2210_neg","Com_2215_neg","Com_2226_neg","Com_2258_neg","Com_2275_neg","Com_2289_neg","Com_2308_neg","Com_2322_neg","Com_2346_neg","Com_2351_neg","Com_2370_neg","Com_2398_neg","Com_2413_neg","Com_2438_neg","Com_2451_neg","Com_2471_neg","Com_2474_neg","Com_2483_neg","Com_2533_neg","Com_2562_neg","Com_2571_neg","Com_2604_neg","Com_2619_neg","Com_2623_neg","Com_2647_neg","Com_2694_neg","Com_2721_neg","Com_2735_neg","Com_2751_neg","Com_2806_neg","Com_2901_neg","Com_2934_neg","Com_2947_neg","Com_2970_neg","Com_3021_neg","Com_3052_neg","Com_3159_neg","Com_3224_neg","Com_3265_neg","Com_3269_neg","Com_3281_neg","Com_3291_neg","Com_3305_neg","Com_3309_neg","Com_3423_neg","Com_3439_neg","Com_3477_neg","Com_3550_neg","Com_3591_neg","Com_3718_neg","Com_3758_neg","Com_3802_neg","Com_3865_neg","Com_3971_neg","Com_4056_neg","Com_4084_neg","Com_4131_neg","Com_4225_neg","Com_4415_neg","Com_4465_neg","Com_4503_neg","Com_4542_neg","Com_4600_neg","Com_4711_neg","Com_4722_neg","Com_4742_neg","Com_4827_neg","Com_4851_neg","Com_4854_neg","Com_4881_neg","Com_4888_neg","Com_4896_neg","Com_4922_neg","Com_4953_neg","Com_4962_neg","Com_4964_neg","Com_5034_neg","Com_5036_neg","Com_5146_neg","Com_5165_neg","Com_5183_neg","Com_5211_neg","Com_5269_neg","Com_5298_neg","Com_5358_neg","Com_5493_neg","Com_5584_neg","Com_5707_neg","Com_5736_neg","Com_5747_neg","Com_5784_neg","Com_5891_neg","Com_5901_neg","Com_5919_neg","Com_6036_neg","Com_6040_neg","Com_6059_neg","Com_6091_neg","Com_6112_neg","Com_6158_neg","Com_6162_neg","Com_6172_neg","Com_6174_neg","Com_6177_neg","Com_6294_neg","Com_6346_neg","Com_6359_neg","Com_6369_neg","Com_6420_neg","Com_6598_neg","Com_6694_neg","Com_6757_neg","Com_6908_neg","Com_6931_neg","Com_6957_neg","Com_7056_neg","Com_7093_neg","Com_7156_neg","Com_7272_neg","Com_7309_neg","Com_7319_neg","Com_7361_neg","Com_7379_neg","Com_7391_neg","Com_7438_neg","Com_7461_neg","Com_7550_neg","Com_7567_neg","Com_7617_neg","Com_7647_neg","Com_7740_neg","Com_7765_neg","Com_7794_neg","Com_7888_neg","Com_7906_neg","Com_7915_neg","Com_7933_neg","Com_7954_neg","Com_8010_neg","Com_8078_neg","Com_8129_neg","Com_8162_neg","Com_8211_neg","Com_8234_neg","Com_8292_neg","Com_8330_neg","Com_8398_neg","Com_8442_neg","Com_8546_neg","Com_8641_neg","Com_8783_neg","Com_8786_neg","Com_8794_neg","Com_8886_neg","Com_8929_neg","Com_9011_neg","Com_9076_neg","Com_9174_neg","Com_9188_neg","Com_9269_neg","Com_9307_neg","Com_9315_neg","Com_9372_neg","Com_9390_neg","Com_9528_neg","Com_9561_neg","Com_9570_neg","Com_9573_neg","Com_9826_neg","Com_10049_neg","Com_10190_neg","Com_10250_neg","Com_10256_neg","Com_10380_neg","Com_10430_neg","Com_10454_neg","Com_10570_neg","Com_10640_neg","Com_11017_neg","Com_11173_neg","Com_11292_neg","Com_11323_neg","Com_11380_neg","Com_11467_neg","Com_11568_neg","Com_11602_neg","Com_11680_neg","Com_11723_neg","Com_11999_neg","Com_12147_neg","Com_12152_neg","Com_12293_neg","Com_12361_neg","Com_12388_neg","Com_12463_neg","Com_12533_neg","Com_12596_neg","Com_12800_neg","Com_12985_neg","Com_13096_neg","Com_13179_neg","Com_13369_neg","Com_13392_neg","Com_13439_neg","Com_13593_neg","Com_13727_neg","Com_13777_neg","Com_13969_neg","Com_14004_neg","Com_14059_neg","Com_14098_neg","Com_14143_neg","Com_14220_neg","Com_14258_neg","Com_14640_neg","Com_14796_neg","Com_14830_neg","Com_14915_neg","Com_15134_neg","Com_15219_neg","Com_15396_neg","Com_15570_neg","Com_15762_neg","Com_15781_neg","Com_15926_neg","Com_16116_neg","Com_16169_neg","Com_16252_neg","Com_16266_neg","Com_16270_neg","Com_16424_neg","Com_16593_neg","Com_16628_neg","Com_16759_neg","Com_16872_neg","Com_17209_neg","Com_17313_neg","Com_17823_neg","Com_17904_neg","Com_18367_neg","Com_18414_neg","Com_18583_neg","Com_19440_neg","Com_19574_neg","Com_19604_neg","Com_19628_neg","Com_19724_neg","Com_19732_neg","Com_19800_neg","Com_19890_neg","Com_20382_neg","Com_20425_neg","Com_20560_neg","Com_20618_neg","Com_20934_neg","Com_23007_neg","Com_23162_neg","Com_23410_neg","Com_23538_neg","Com_24132_neg","Com_24247_neg","Com_24414_neg","Com_24685_neg","Com_3307_neg","Com_48_neg","Com_29_neg","Com_1658_neg","Com_3135_neg","Com_1301_neg","Com_12532_neg","Com_130_neg","Com_114_neg","Com_265_neg","Com_353_neg","Com_150_neg","Com_328_neg","Com_90_neg","Com_583_neg","Com_672_neg","Com_594_neg","Com_51_neg","Com_7692_neg","Com_1144_neg","Com_1045_neg","Com_1519_neg","Com_1697_neg","Com_802_neg","Com_2408_neg","Com_1672_neg","Com_652_neg","Com_10723_neg","Com_507_neg","Com_377_neg","Com_821_neg","Com_781_neg","Com_1293_neg","Com_1020_neg","Com_3328_neg","Com_1260_neg","Com_2191_neg","Com_1677_neg","Com_2268_neg","Com_1795_neg","Com_2266_neg","Com_91_neg","Com_1485_neg","Com_2893_neg","Com_1996_neg","Com_317_neg","Com_570_neg","Com_2039_neg","Com_4183_neg","Com_1086_neg","Com_401_neg","Com_1297_neg","Com_3817_neg","Com_762_neg","Com_1718_neg","Com_786_neg","Com_6221_neg","Com_877_neg","Com_447_neg","Com_2081_neg","Com_2887_neg","Com_3533_neg","Com_3830_neg","Com_3906_neg","Com_699_neg","Com_2672_neg","Com_7240_neg","Com_3852_neg","Com_4687_neg","Com_4254_neg","Com_4341_neg","Com_2236_neg","Com_6187_neg","Com_6869_neg","Com_222_neg","Com_7962_neg","Com_1461_neg","Com_11395_neg","Com_2725_neg","Com_9663_neg","Com_19462_neg","Com_6945_neg","Com_4005_neg","Com_1497_neg","Com_8114_neg","Com_221_neg","Com_15819_neg","Com_8932_neg","Com_6342_neg","Com_5484_neg","Com_10_pos","Com_13_pos","Com_24_pos","Com_41_pos","Com_45_pos","Com_49_pos","Com_71_pos","Com_78_pos","Com_88_pos","Com_113_pos","Com_114_pos","Com_188_pos","Com_208_pos","Com_270_pos","Com_312_pos","Com_315_pos","Com_337_pos","Com_379_pos","Com_447_pos","Com_459_pos","Com_493_pos","Com_539_pos","Com_557_pos","Com_828_pos","Com_1160_pos","Com_1438_pos","Com_1573_pos","Com_1687_pos","Com_1835_pos","Com_1839_pos","Com_2095_pos","Com_2189_pos","Com_2769_pos","Com_3204_pos","Com_3226_pos","Com_3919_pos","Com_4165_pos","Com_9636_pos","Com_10332_pos","Com_1_pos","Com_12_pos","Com_28_pos","Com_35_pos","Com_38_pos","Com_39_pos","Com_67_pos","Com_164_pos","Com_223_pos","Com_276_pos","Com_360_pos","Com_813_pos","Com_842_pos","Com_1064_pos","Com_1189_pos","Com_1383_pos","Com_1445_pos","Com_1588_pos","Com_1873_pos","Com_2320_pos","Com_3407_pos","Com_7015_pos","Com_12322_pos","Com_23_pos","Com_33_pos","Com_37_pos","Com_40_pos","Com_44_pos","Com_53_pos","Com_79_pos","Com_94_pos","Com_130_pos","Com_131_pos","Com_133_pos","Com_136_pos","Com_153_pos","Com_162_pos","Com_202_pos","Com_221_pos","Com_243_pos","Com_273_pos","Com_297_pos","Com_350_pos","Com_355_pos","Com_376_pos","Com_384_pos","Com_424_pos","Com_428_pos","Com_441_pos","Com_470_pos","Com_486_pos","Com_491_pos","Com_497_pos","Com_503_pos","Com_508_pos","Com_536_pos","Com_545_pos","Com_562_pos","Com_591_pos","Com_601_pos","Com_609_pos","Com_610_pos","Com_643_pos","Com_644_pos","Com_700_pos","Com_769_pos","Com_802_pos","Com_818_pos","Com_832_pos","Com_861_pos","Com_876_pos","Com_912_pos","Com_915_pos","Com_929_pos","Com_947_pos","Com_956_pos","Com_970_pos","Com_972_pos","Com_1045_pos","Com_1052_pos","Com_1062_pos","Com_1070_pos","Com_1089_pos","Com_1098_pos","Com_1104_pos","Com_1113_pos","Com_1127_pos","Com_1142_pos","Com_1144_pos","Com_1145_pos","Com_1187_pos","Com_1234_pos","Com_1259_pos","Com_1271_pos","Com_1277_pos","Com_1288_pos","Com_1311_pos","Com_1332_pos","Com_1345_pos","Com_1352_pos","Com_1362_pos","Com_1378_pos","Com_1434_pos","Com_1468_pos","Com_1481_pos","Com_1488_pos","Com_1499_pos","Com_1501_pos","Com_1515_pos","Com_1543_pos","Com_1551_pos","Com_1559_pos","Com_1584_pos","Com_1610_pos","Com_1658_pos","Com_1679_pos","Com_1719_pos","Com_1745_pos","Com_1761_pos","Com_1775_pos","Com_1782_pos","Com_1795_pos","Com_1799_pos","Com_1801_pos","Com_1811_pos","Com_1815_pos","Com_1824_pos","Com_1864_pos","Com_1866_pos","Com_1869_pos","Com_1887_pos","Com_1898_pos","Com_1908_pos","Com_1910_pos","Com_1942_pos","Com_1949_pos","Com_1967_pos","Com_1981_pos","Com_1998_pos","Com_2031_pos","Com_2037_pos","Com_2042_pos","Com_2060_pos","Com_2070_pos","Com_2105_pos","Com_2158_pos","Com_2168_pos","Com_2209_pos","Com_2213_pos","Com_2270_pos","Com_2289_pos","Com_2323_pos","Com_2353_pos","Com_2355_pos","Com_2404_pos","Com_2496_pos","Com_2507_pos","Com_2514_pos","Com_2530_pos","Com_2574_pos","Com_2586_pos","Com_2612_pos","Com_2696_pos","Com_2706_pos","Com_2707_pos","Com_2722_pos","Com_2726_pos","Com_2744_pos","Com_2785_pos","Com_2813_pos","Com_2831_pos","Com_2834_pos","Com_2844_pos","Com_2849_pos","Com_2854_pos","Com_2860_pos","Com_2862_pos","Com_2894_pos","Com_2905_pos","Com_2907_pos","Com_2913_pos","Com_2920_pos","Com_2925_pos","Com_3010_pos","Com_3020_pos","Com_3024_pos","Com_3065_pos","Com_3072_pos","Com_3080_pos","Com_3084_pos","Com_3095_pos","Com_3166_pos","Com_3243_pos","Com_3319_pos","Com_3326_pos","Com_3327_pos","Com_3333_pos","Com_3357_pos","Com_3366_pos","Com_3382_pos","Com_3392_pos","Com_3399_pos","Com_3413_pos","Com_3420_pos","Com_3436_pos","Com_3456_pos","Com_3494_pos","Com_3502_pos","Com_3505_pos","Com_3506_pos","Com_3529_pos","Com_3574_pos","Com_3617_pos","Com_3666_pos","Com_3711_pos","Com_3730_pos","Com_3754_pos","Com_3762_pos","Com_3780_pos","Com_3781_pos","Com_3821_pos","Com_3834_pos","Com_3873_pos","Com_3894_pos","Com_3895_pos","Com_3933_pos","Com_3942_pos","Com_3946_pos","Com_3974_pos","Com_3981_pos","Com_3984_pos","Com_4019_pos","Com_4031_pos","Com_4039_pos","Com_4042_pos","Com_4064_pos","Com_4100_pos","Com_4102_pos","Com_4117_pos","Com_4140_pos","Com_4157_pos","Com_4159_pos","Com_4184_pos","Com_4204_pos","Com_4250_pos","Com_4256_pos","Com_4284_pos","Com_4295_pos","Com_4297_pos","Com_4300_pos","Com_4320_pos","Com_4376_pos","Com_4391_pos","Com_4415_pos","Com_4435_pos","Com_4448_pos","Com_4485_pos","Com_4535_pos","Com_4554_pos","Com_4557_pos","Com_4570_pos","Com_4580_pos","Com_4638_pos","Com_4651_pos","Com_4663_pos","Com_4671_pos","Com_4696_pos","Com_4703_pos","Com_4708_pos","Com_4718_pos","Com_4745_pos","Com_4779_pos","Com_4781_pos","Com_4786_pos","Com_4796_pos","Com_4798_pos","Com_4837_pos","Com_4869_pos","Com_4903_pos","Com_4923_pos","Com_4953_pos","Com_5038_pos","Com_5062_pos","Com_5071_pos","Com_5146_pos","Com_5222_pos","Com_5252_pos","Com_5279_pos","Com_5377_pos","Com_5425_pos","Com_5428_pos","Com_5471_pos","Com_5486_pos","Com_5491_pos","Com_5495_pos","Com_5601_pos","Com_5617_pos","Com_5643_pos","Com_5673_pos","Com_5705_pos","Com_5782_pos","Com_5804_pos","Com_5825_pos","Com_5829_pos","Com_5850_pos","Com_5883_pos","Com_5965_pos","Com_5991_pos","Com_6040_pos","Com_6100_pos","Com_6158_pos","Com_6182_pos","Com_6222_pos","Com_6251_pos","Com_6257_pos","Com_6265_pos","Com_6336_pos","Com_6375_pos","Com_6411_pos","Com_6413_pos","Com_6429_pos","Com_6440_pos","Com_6447_pos","Com_6463_pos","Com_6587_pos","Com_6591_pos","Com_6621_pos","Com_6675_pos","Com_6870_pos","Com_6959_pos","Com_6963_pos","Com_6976_pos","Com_7065_pos","Com_7068_pos","Com_7142_pos","Com_7171_pos","Com_7209_pos","Com_7240_pos","Com_7290_pos","Com_7305_pos","Com_7330_pos","Com_7332_pos","Com_7352_pos","Com_7405_pos","Com_7411_pos","Com_7433_pos","Com_7459_pos","Com_7475_pos","Com_7492_pos","Com_7510_pos","Com_7567_pos","Com_7600_pos","Com_7660_pos","Com_7820_pos","Com_7825_pos","Com_7856_pos","Com_7868_pos","Com_7873_pos","Com_7894_pos","Com_7961_pos","Com_8024_pos","Com_8029_pos","Com_8055_pos","Com_8117_pos","Com_8165_pos","Com_8236_pos","Com_8285_pos","Com_8303_pos","Com_8330_pos","Com_8340_pos","Com_8380_pos","Com_8414_pos","Com_8431_pos","Com_8439_pos","Com_8454_pos","Com_8464_pos","Com_8531_pos","Com_8562_pos","Com_8567_pos","Com_8591_pos","Com_8669_pos","Com_8687_pos","Com_8731_pos","Com_8736_pos","Com_8749_pos","Com_8750_pos","Com_8762_pos","Com_8783_pos","Com_8800_pos","Com_8804_pos","Com_8830_pos","Com_8835_pos","Com_8846_pos","Com_8851_pos","Com_8900_pos","Com_8957_pos","Com_8973_pos","Com_8990_pos","Com_9247_pos","Com_9284_pos","Com_9299_pos","Com_9303_pos","Com_9330_pos","Com_9342_pos","Com_9359_pos","Com_9361_pos","Com_9430_pos","Com_9443_pos","Com_9447_pos","Com_9530_pos","Com_9593_pos","Com_9675_pos","Com_9764_pos","Com_9850_pos","Com_9861_pos","Com_9962_pos","Com_9966_pos","Com_9969_pos","Com_10019_pos","Com_10029_pos","Com_10104_pos","Com_10122_pos","Com_10144_pos","Com_10159_pos","Com_10192_pos","Com_10219_pos","Com_10231_pos","Com_10249_pos","Com_10351_pos","Com_10367_pos","Com_10383_pos","Com_10384_pos","Com_10461_pos","Com_10500_pos","Com_10513_pos","Com_10514_pos","Com_10545_pos","Com_10575_pos","Com_10700_pos","Com_10730_pos","Com_10731_pos","Com_10750_pos","Com_10761_pos","Com_10795_pos","Com_10897_pos","Com_10911_pos","Com_10929_pos","Com_10960_pos","Com_11035_pos","Com_11087_pos","Com_11102_pos","Com_11164_pos","Com_11208_pos","Com_11263_pos","Com_11398_pos","Com_11403_pos","Com_11452_pos","Com_11464_pos","Com_11496_pos","Com_11534_pos","Com_11581_pos","Com_11708_pos","Com_11710_pos","Com_11722_pos","Com_11937_pos","Com_11945_pos","Com_11952_pos","Com_11961_pos","Com_12100_pos","Com_12133_pos","Com_12140_pos","Com_12195_pos","Com_12276_pos","Com_12352_pos","Com_12375_pos","Com_12465_pos","Com_12507_pos","Com_12570_pos","Com_12645_pos","Com_12736_pos","Com_12756_pos","Com_12779_pos","Com_12972_pos","Com_13014_pos","Com_13071_pos","Com_13192_pos","Com_13214_pos","Com_13288_pos","Com_13440_pos","Com_13455_pos","Com_13515_pos","Com_13571_pos","Com_13632_pos","Com_13651_pos","Com_13654_pos","Com_13696_pos","Com_13799_pos","Com_13818_pos","Com_13836_pos","Com_13841_pos","Com_13845_pos","Com_13949_pos","Com_13950_pos","Com_13992_pos","Com_14051_pos","Com_14052_pos","Com_14173_pos","Com_14191_pos","Com_14245_pos","Com_14250_pos","Com_14307_pos","Com_14359_pos","Com_14379_pos","Com_14553_pos","Com_14570_pos","Com_14651_pos","Com_14773_pos","Com_14874_pos","Com_14881_pos","Com_14897_pos","Com_14948_pos","Com_15011_pos","Com_15016_pos","Com_15054_pos","Com_15099_pos","Com_15234_pos","Com_15303_pos","Com_15305_pos","Com_15362_pos","Com_15391_pos","Com_15470_pos","Com_15667_pos","Com_15677_pos","Com_15737_pos","Com_15755_pos","Com_15888_pos","Com_15902_pos","Com_15930_pos","Com_16007_pos","Com_16091_pos","Com_16139_pos","Com_16257_pos","Com_16406_pos","Com_16430_pos","Com_16494_pos","Com_16510_pos","Com_16520_pos","Com_16601_pos","Com_16747_pos","Com_16808_pos","Com_16831_pos","Com_16977_pos","Com_17257_pos","Com_17330_pos","Com_17334_pos","Com_17581_pos","Com_17631_pos","Com_17674_pos","Com_17717_pos","Com_17897_pos","Com_18042_pos","Com_18269_pos","Com_18282_pos","Com_18283_pos","Com_18315_pos","Com_18328_pos","Com_18342_pos","Com_18513_pos","Com_18624_pos","Com_18643_pos","Com_18647_pos","Com_18695_pos","Com_18712_pos","Com_18837_pos","Com_18842_pos","Com_18893_pos","Com_18997_pos","Com_19012_pos","Com_19086_pos","Com_19133_pos","Com_19158_pos","Com_19619_pos","Com_19700_pos","Com_19746_pos","Com_19782_pos","Com_19857_pos","Com_19928_pos","Com_19960_pos","Com_20003_pos","Com_20110_pos","Com_20184_pos","Com_20461_pos","Com_20722_pos","Com_20821_pos","Com_20948_pos","Com_21062_pos","Com_21093_pos","Com_21129_pos","Com_21186_pos","Com_21251_pos","Com_21314_pos","Com_21415_pos","Com_21443_pos","Com_21729_pos","Com_21769_pos","Com_21938_pos","Com_21952_pos","Com_21960_pos","Com_22085_pos","Com_22137_pos","Com_22142_pos","Com_22230_pos","Com_22235_pos","Com_22244_pos","Com_22280_pos","Com_22787_pos","Com_22923_pos","Com_22960_pos","Com_23013_pos","Com_23030_pos","Com_23051_pos","Com_23131_pos","Com_23212_pos","Com_23256_pos","Com_23272_pos","Com_23308_pos","Com_23541_pos","Com_23580_pos","Com_23602_pos","Com_23638_pos","Com_23767_pos","Com_24091_pos","Com_24140_pos","Com_24344_pos","Com_24688_pos","Com_24740_pos","Com_24994_pos","Com_25067_pos","Com_25107_pos","Com_25109_pos","Com_25133_pos","Com_25465_pos","Com_25519_pos","Com_25537_pos","Com_25540_pos","Com_25704_pos","Com_25743_pos","Com_25835_pos","Com_25858_pos","Com_26094_pos","Com_26101_pos","Com_26202_pos","Com_26329_pos","Com_26519_pos","Com_26566_pos","Com_26660_pos","Com_26793_pos","Com_26901_pos","Com_26930_pos","Com_27032_pos","Com_27134_pos","Com_27305_pos","Com_27318_pos","Com_27368_pos","Com_27590_pos","Com_27893_pos","Com_27991_pos","Com_28024_pos","Com_28060_pos","Com_28270_pos","Com_28590_pos","Com_28603_pos","Com_28853_pos","Com_28921_pos","Com_28963_pos","Com_29104_pos","Com_29354_pos","Com_29536_pos","Com_29573_pos","Com_29691_pos","Com_29707_pos","Com_30030_pos","Com_30647_pos","Com_30714_pos","Com_30886_pos","Com_30897_pos","Com_31000_pos","Com_31019_pos","Com_31123_pos","Com_31283_pos","Com_31909_pos","Com_32486_pos","Com_32504_pos","Com_32619_pos","Com_33031_pos","Com_33384_pos","Com_33548_pos","Com_33885_pos","Com_33924_pos","Com_33947_pos","Com_34217_pos","Com_34869_pos","Com_34966_pos","Com_35410_pos","Com_35821_pos","Com_36051_pos","Com_36235_pos","Com_36984_pos","Com_37148_pos","Com_37386_pos","Com_37836_pos","Com_38105_pos","Com_38329_pos","Com_38572_pos","Com_38858_pos","Com_25_pos","Com_51_pos","Com_56_pos","Com_65_pos","Com_72_pos","Com_77_pos","Com_91_pos","Com_92_pos","Com_107_pos","Com_144_pos","Com_145_pos","Com_149_pos","Com_154_pos","Com_155_pos","Com_158_pos","Com_175_pos","Com_186_pos","Com_219_pos","Com_228_pos","Com_244_pos","Com_262_pos","Com_300_pos","Com_309_pos","Com_316_pos","Com_328_pos","Com_405_pos","Com_442_pos","Com_450_pos","Com_456_pos","Com_458_pos","Com_480_pos","Com_543_pos","Com_560_pos","Com_578_pos","Com_611_pos","Com_625_pos","Com_705_pos","Com_744_pos","Com_765_pos","Com_784_pos","Com_811_pos","Com_823_pos","Com_826_pos","Com_836_pos","Com_848_pos","Com_862_pos","Com_896_pos","Com_944_pos","Com_992_pos","Com_994_pos","Com_1003_pos","Com_1167_pos","Com_1169_pos","Com_1223_pos","Com_1229_pos","Com_1242_pos","Com_1301_pos","Com_1307_pos","Com_1391_pos","Com_1394_pos","Com_1433_pos","Com_1457_pos","Com_1470_pos","Com_1474_pos","Com_1626_pos","Com_1644_pos","Com_1654_pos","Com_1726_pos","Com_1812_pos","Com_1920_pos","Com_1966_pos","Com_2021_pos","Com_2085_pos","Com_2089_pos","Com_2203_pos","Com_2212_pos","Com_2215_pos","Com_2237_pos","Com_2255_pos","Com_2274_pos","Com_2416_pos","Com_2521_pos","Com_2539_pos","Com_2596_pos","Com_2633_pos","Com_2680_pos","Com_2709_pos","Com_2753_pos","Com_2778_pos","Com_2868_pos","Com_2889_pos","Com_2890_pos","Com_2966_pos","Com_3057_pos","Com_3073_pos","Com_3153_pos","Com_3158_pos","Com_3229_pos","Com_3240_pos","Com_3255_pos","Com_3346_pos","Com_3365_pos","Com_3373_pos","Com_3667_pos","Com_3750_pos","Com_4512_pos","Com_4655_pos","Com_4867_pos","Com_4913_pos","Com_4947_pos","Com_4998_pos","Com_5081_pos","Com_5482_pos","Com_5570_pos","Com_5765_pos","Com_6110_pos","Com_6153_pos","Com_6180_pos","Com_6342_pos","Com_6397_pos","Com_6402_pos","Com_6404_pos","Com_6610_pos","Com_6611_pos","Com_6685_pos","Com_6983_pos","Com_6987_pos","Com_7005_pos","Com_7180_pos","Com_7398_pos","Com_7423_pos","Com_7729_pos","Com_7818_pos","Com_8220_pos","Com_8278_pos","Com_8886_pos","Com_9340_pos","Com_10472_pos","Com_10623_pos","Com_10626_pos","Com_11050_pos","Com_11125_pos","Com_11869_pos","Com_12774_pos","Com_13238_pos","Com_13449_pos","Com_13659_pos","Com_14627_pos","Com_15282_pos","Com_15426_pos","Com_15504_pos","Com_15917_pos","Com_16178_pos","Com_16407_pos","Com_16554_pos","Com_16618_pos","Com_16974_pos","Com_17934_pos","Com_18531_pos","Com_19779_pos","Com_21285_pos","Com_21414_pos","Com_21758_pos","Com_22449_pos","Com_23158_pos","Com_23975_pos","Com_24151_pos","Com_25286_pos","Com_25995_pos","Com_28811_pos","Com_29019_pos","Com_29050_pos","Com_29453_pos","Com_32136_pos","Com_34461_pos"]}}