MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/m_MTBLS14012_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/m_MTBLS14012_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/a_MTBLS14012_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/s_MTBLS14012.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012/a_MTBLS14012_LC-MS_negative_reverse-phase_metabolite_profiling.txtprimary200OKftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14012<p>And then peakswerematched with the mzCloud</p><p>(https://www.mzcloud.org/), mzVault and MassList database to obtain the accuratequalitative and relative quantitative results.Statistical analyses were performed usingthe statistical software R(R version R-3.4.3),Python (Python 2.7.6 version) andCentOS (CentOS release 6.6),When data were not normally distributed, normaltransformations were attempted using of area normalization method</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>UHPLC-MS/MS analyses were performed using a Vanquish UHPLC system (Thermo Fisher, Germany) coupled with an Orbitrap Q Exactive™ HF-X mass spectrometer (Thermo Fisher, Germany) at Novogene Co., Ltd. (Beijing, China). Samples were injected onto a Hypersil Gold column (100 × 2.1 mm, 1.9 µm) using a 17-minute linear gradient at a flow rate of 0.2 mL/min. The eluents for the positive polarity mode were eluent A (0.1% FA in water) and eluent B (methanol). The eluents for the negative polarity mode were eluent A (5 mM ammonium acetate, pH 9.0) and eluent B (methanol). The solvent gradient was set as follows: 2% B, 1.5 min; 2–100% B, 3 min; 100% B, 10 min; 100–2% B, 10.1 min; 2% B, 12 min.</p>Deletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses.Capital Medical Universityyuqing sunMyceliummass spectrometry assay<p>The samples were placed in the EP tubes and resuspended with prechilled 80%methanol by well vortex. Then the samples were melted on ice and whirled for 30 s.After the sonification for 6 min, they were centrifuged at 5,000 rpm, 4°C for 1 minThe supernatant was freeze-dried and dissolvedwith 10% methanol. Finally, thesolution was injected into the LC-MS/MS system analysis</p>Aspergillus fumigatushttps://www.ebi.ac.uk/metabolights/MTBLS14012Yuqing Sun. Chinese Center For Disease Control and Prevention. sunyq0519@163.com.<p>The raw data files generated by UHPLC-MS/MS were processed using the CompoundDiscoverer 3.1 (CD3.1, ThermoFisher) to perform peak alignment, peak picking, andquantitation for each metabolite. The main parameterswere set as follows: retentiontime tolerance, 0.2 minutes; actual mass tolerance, Sppm; signal intensity tolerance30%; signalnoise ratio, 3; and minimum intensity, et al. After that, peak intensitieswere normalized to the total spectral intensity.The normalized data was used topredict the molecular formula based on additive ions, molecular ion peaks andions.</p>Treatmentsunyq0519@163.com<p>After culturing&nbsp;Aspergillus fumigatus&nbsp;for 24 hours, the culture was centrifuged at 8000 rpm for 10 minutes. The supernatant was discarded, and the pellet was immediately frozen in liquid nitrogen.</p>MetabolomicsmyceliumgliotoxinAspergillus fumigatusImmunityuntargeted metabolite profilingmyceliumgliotoxinAspergillus fumigatusImmunityuntargeted metabolite profiling<p>QExactiveTM HlF-X mass spectrometer was operated in positive/negative polarity modewith spray voltage of 3.5 kV, capillary temperature of 320°C, sheath gas flow rate of35 psi and aux gas flow rate of 10 L/min, S-lens RF level of 60, Aux gas heatertemperature of350°C</p>3-(4-ethoxyphenyl)-2-(tetrahydro-1H-pyrrol-1-ylcarbonyl)acrylonitrileN~1~-(2,5-dimethoxyphenyl)-N~2~-(2-pyridin-2-ylethyl)ethanediamideHNHHNKProstaglandin G2DL-PanthenolMonolaurin2-(benzylamino)-6-methyl-5-(2-methyl-1,3-thiazol-4-yl)nicotinonitrile13(S)-HOTrEN-Acetyl-L-histidineU-47700-d6Imidazolelactic acidStearoyl ethanolamideDl-3,4-Dihydroxymandelic AcidLysopa 16:02-[(3S)-1-(2-Thienylmethyl)-3-pyrrolidinyl]-1,3-benzoxazole8-Aminooctanoic acidWLKACar 5:0PC (18:2/18:3)5-Hydroxymethyl-2-furancarboxylic acidL-HistidineIsobutyryl carnitine(2E)-3-phenyl-N-(2-phenylethyl)prop-2-enamideNormorphine2-Arachidonoyl glycerol4-[4-(1-phenyl-1H-1,2,4-triazol-5-yl)-1,3-thiazol-2-yl]pyridineL-TyrosinemethylesterD-Sphingosine2-(3,4-dihydroxyphenyl)-7-hydroxy-3,4-dihydro-2H-1-benzopyran-4-oneethyl 2-[3-oxo-5,6-diphenyl-1,2,4-triazin-2(3H)-yl]acetate2,3-Dinor-TXB23-Hydroxyanthranilic AcidYQHTropolone6-Methoxy-2-naphthoic acidN-Acetyl-D-tryptophanHPHHPKFLK7-(2-aminophenyl)heptanoic acid1-(4-benzoylpiperidino)-2-[(4-chlorophenyl)thio]ethan-1-oneProstaglandin E2MeclocyclineProline-hydroxyprolineProstaglandin E12-(1,3-benzodioxol-5-yl)-7-ethylimidazo[1,2-a]pyridineN6-Me-dA20-Carboxy-Leukotriene B4N6-Acetyl-L-lysineWNK5,6-dihydroxyindole3-(propan-2-yl)-octahydropyrrolo[1,2-a]pyrazine-1,4-dione2-hydroxy-6-[(8Z,11Z)-pentadeca-8,11,14-trien-1-yl]benzoic acidBoldioneHQHN-benzyl-N-isopropyl-N'-[4-(trifluoromethoxy)phenyl]ureaN1,N1-dimethyl-4-fluoro-2-nitro-5-tetrahydro-1H-pyrrol-1-ylanilineGlutaric AcidOxohongdenafil4-(5-propyl-2-pyridyl)benzonitrilePC (16:1e/2:0)D-(+)-Maltose(+/-)12(13)-DiHOMEFexofenadineIsophorone4-Methyl-5-thiazoleethanolD-(-)-Aspartic acid1,4-Dihydro-1-Methyl-4-Oxo-3-PyridinecarboxamideN-(4-piperidinophenyl)benzamideThiazolidine-4-carboxylic acidN-Acetylneuraminic acidFNKIndoxylsulfuric acidVindoline4-(cyclohexylmethyl)-6-(2-thienyl)-2,3-dihydropyridazin-3-one hydrate3-hydroxy-2-octylpentanedioic acidPheniramine N-oxide5-fluoro AB-PINACA N-(4-hydroxypentyl) metabolite7-KetocholesterolL-OrnithinePC (17:0/18:2)5,6-dimethyl-4-oxo-4H-pyran-2-carboxylic acid4-Morpholinepropanesulfonic acid5,6-Dihydroxyindole-2-Carboxylic AcidN-FormylkynurenineNeosaxitoxinS-LactoyglutathioneValerophenoneEthyl chrysanthemumateOxaceprolDKK4-oxododecanedioic acidThymopentinMupirocin1-Phenyl-3-methyl-5-pyrazoloneAsp-glu1-(4-methylphenyl)-3,5-diphenylpent-2-ene-1,5-dione8Z,11Z,14Z-Eicosatrienoic acid(12Z)-9,10,11-trihydroxyoctadec-12-enoic acid5-Henicosyl-1,3-benzenediolPC (7:0/8:0)UMP2-PhenylglycineAndrostenedione4-(4-hydroxy-3,5-dimethylphenyl)-2-methylphthalazin-1(2H)-one(2-morpholino-5-nitrophenyl)methanolFPHFPK4,4'-dimethoxy[1,1'-biphenyl]-2-carbonitrilePC (14:1e/5:0)DLKIndoleOctadeca-11E,13E,15Z-trienoic acidDL-Arginine10-Nitrolinoleate4-(2,3-dihydro-1,4-benzodioxin-6-yl)butanoic acidDL-3,4-Dihydroxyphenyl glycolL-CarnitineL-Hydroxylysine4,6,8-trihydroxy-7-methoxy-3-methyl-3,4-dihydro-1H-2-benzopyran-1-oneFQHo-Cresol1-(1,3-benzodioxol-5-yl)-2-[4-(9H-fluoren-9-yl)piperazino]-1-ethanoneDMKPE (18:2/18:2)Gly-Tyr-AlaN-DesmethylclobazamDL-Norvaline3,14-dihydro-15-keto-tetranor Prostaglandin E22-Hydroxycinnamic acid23-Norcholic acidSKK6-(benzylamino)-3-phenyl-1,4-dihydropyridazin-4-one2-(14,15-Epoxyeicosatrienoyl) glycerolD-Erythrose 4-phosphate3,4,5-trihydroxy-6-methyloxan-2-yl 2-(methylamino)benzoate2-[5-(2-hydroxypropyl)oxolan-2-yl]propanoic acidFRHDNK2-DeoxyuridineNorfludiazepamPC (14:1e/3:0)L-Phenylalanine3-[1-(dimethylamino)-1H-pyrrol-2-yl]-2-(2-furylcarbonyl)acrylonitrile5-MethylcytosineAla-LeuDocosahexaenoic acidThioguanineIsoproterenolSLH2,5-bis(4-hydroxy-3-methoxyphenyl)-3,4-dimethyloxolan-3-olOleanolic acidLPE 20:1LPE 20:26 ¦Â-Hydroxycortisol(5-hydroxy-3-methyl-1-phenyl-1H-pyrazol-4-yl)(phenyl)methanoneN'3-(3,4,5-trimethoxybenzylidene)pyridine-3-carbohydrazideSLKtert-Butyl N-[1-(aminocarbonyl)-3-methylbutyl]carbamate(+)-Evodiaminegamma-Glutamylleucinegamma-GlutamylmethionineTetrahydrocortisoneCelestolide2-(cyclopropylimino)-3-(2-thienylmethyl)-1,3-thiazolan-4-oneNitrazepam-d5SMHSMK6-Sialyllactose12,13-EODEDPHDPKDL-Tryptophan4-(3-Methyl-5-oxo-4,5-dihydro-1H-pyrazol-1-yl)benzenesulfonic acidcis-7-Hexadecenoic Acid6-benzyl-4-oxo-1,4-dihydropyridine-3-carboxamide2,3,4-Trihydroxybenzoic acidSNHL-Glutamic acid monosodium saltPC (16:2e/4:0)Guanine2-NaphtholSNKN-Acetyl-L-carnosineAdenosine 5'-DiphosphatePenta-N-acetylchitopentaose(3S,9aS)-3-benzyl-octahydro-1H-pyrido[1,2-a]pyrazin-1-oneLysopg 18:1Uridine 5'-monophosphate1,4-dihydroxyheptadec-16-en-2-yl acetateQKK7-MethylguanineBicyclo prostaglandin E26-(3-hydroxybutan-2-yl)-5-(hydroxymethyl)-4-methoxy-2H-pyran-2-oneN-{[(2R,4S,5R)-5-Ethyl-1-azabicyclo[2.2.2]oct-2-yl]methyl}-2-furamide3-(4-hydroxy-3-methoxyphenyl)propanoic acid(3beta,9xi)-3-(beta-D-Glucopyranosyloxy)-14-hydroxycard-20(22)-enolideDRHProstaglandin K211-Epiprostaglandin E1MJN1101-(7-methoxy-2-oxo-2H-chromen-8-yl)-3-methyl-2-oxobutyl acetateSPHQLH4-[4-(trifluoromethoxy)anilino]-2H-1,3-benzoxazin-2-oneAdenosine 3'5'-cyclic monophosphatePipecolic acidSPKIndole-3-acrylic acidQLK4-(4-methoxyphenyl)-2-(2-thienyl)pyrimidine2'-Deoxyuridine15-epi Prostaglandin A1Cinnamoylglycine4-(3,4-dihydro-2H-1,5-benzodioxepin-7-ylamino)-4-oxobutanoic acidBisphenol GQMK(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-(2-phenylethoxy)oxane-3,4,5-triol6-cyclohexyl-5-(cyclohexylimino)-4-thia-6-azaspiro[2.4]heptan-7-one2-{[(4,5-dimethoxy-2-nitrophenethyl)imino]methyl}phenol3-hydroxy-4-(3-hydroxyphenyl)-1,2-dihydroquinolin-2-oneOfloxacin impurity ETaurochenodeoxycholic acid2'-Deoxyadenosine-5'-monophosphateEcgonine methyl ester2-[(7-methyl-2,3-dihydro-1H-inden-4-yl)oxy]pyridin-3-amine5-HydroxymebendazoleN3,N4-Dimethyl-L-arginineTHJ2201 N-(5-hydroxypentyl) metaboliteCafestolJ1472-Benzyl-5-[(3S)-1-isopropyl-3-pyrrolidinyl]-1,3,4-oxadiazoleQNKN1-{4-methyl-2-[(3,4,5-trimethoxybenzylidene)amino]phenyl}acetamidecis-Aconitic acidN epsilon,N epsilon,N epsilon-trimethyllysine(2E,4E)-N-[2-(4-hydroxyphenyl)ethyl]dodeca-2,4-dienamideProstaglandin J2Thymine9,10-DihomePC (14:0e/3:0)Physostigmine2-[(3S)-1-(2-Pyridinylmethyl)-3-pyrrolidinyl]-1,3-benzothiazoleStercobilinOleoyl-L-alpha-lysophosphatidic acid1-MethyladenosineN-Acetylglucosamine 1-phosphate4-HydroxyisoleucineL-Cysteine-glutathione gisulfide1-MethylhistamineCoenzyme Q2Gamma-Glu-LeuQPH4-(4-methoxyphenyl)-6-pyridin-4-yl-1,3,5-triazin-2(3H)-one3-Hydroxy-3-methylbutanoic acidLithocholic acid2-({2-[(3-methyl-5-cinnolinyl)amino]-2-oxoethyl}sulfanyl)acetic acidPro-Leu5-methoxy-8,8-dimethyl-2-phenyl-4H,8H-pyrano[2,3-h]chromen-4-oneN-AcetylsphingosineL-ThreonineGuanosine 3',5'-cyclic monophosphateo-Toluic AcidOxolamineD-Alanyl-D-alanineLDGTS 16:0Andrographolide3-(5-phenyl-1,3-oxazol-2-yl)-4-(trifluoromethyl)pyridineLSD-d35-phenyl-2,3-dihydro-1H-1,4-benzodiazepin-2-onePyridoxalN-Acetyl-Asp-Glu5,8-dihydroxy-10-methyl-5,8,9,10-tetrahydro-2H-oxecin-2-one(+/-)11(12)-EET(1E)-5-hydroxy-1,7-diphenylhept-1-en-3-oneN-PropionylglycineN6-Succinyl Adenosinediethyl 2-[(4-benzhydrylpiperidino)methylidene]malonate11-Dehydro thromboxane B2Lysopc 16:1R-1 Methanandamide phosphateMF4982,3-dihydroxypropyl 12-methyltridecanoateL-PalmitoylcarnitineL-TryptophanOrlistatPC (16:0e/3:0)13,14-Dihydro-15-keto Prostaglandin A2N-Tetradecanamide4-Guanidinobutanoic acidgamma-TocopherolGlycitinL-Leucyl-L-alanine HydrateW-15-d4TestosteroneO-DesmethylnaproxenN1-[4-hydroxy-6-(methoxymethyl)pyrimidin-2-yl]acetamideEicosapentaenoic acidUracilCuminaldehydeL-Kynurenine(2,6-dimethylmorpholino)(1-methyl-5-nitro-1H-pyrazol-4-yl)methanone15-Acetyldeoxynivalenol1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene acetaldehydeDesmethylclozapine2-[2-(2,6-Dimethylmorpholino)-2-oxoethoxy]phenyl N,N-dimethylcarbamate16-Heptadecyne-1,2,4-triolCytosineCiprosteneLPC 16:2LPC 16:011-Deoxy prostaglandin F1¦ÁMMKN-Acetyl-5-aminosalicylic acidN-acetyl-D-glucosamineProstaglandin E2-1-glyceryl esterN-acetyl-L-ornithinePC (17:1/17:2)LPE 19:1N1-cyclooctyl-4-hydroxy-1-piperidinecarbothioamidePE (17:1/17:2)N-(4-fluorophenyl)-N'-(2-piperidinophenyl)ureaMNK2,6-DihydroxypurineNicotinuric AcidFlurazepam-d104-HydroxytamoxifenL-Glutamic acid3-(methylsulfonyl)-2H-chromen-2-oneKKK4-oxo-4-[(1-phenylethyl)amino]but-2-enoic aciddAMP2-(2-thienyl)-4H-chromen-4-oneErgosta-5,7,9(11),22-Tetraen-3-beta-OldopaquinonePalmitoleic acid3-benzyl-1-methyl-2-oxo-1,2-dihydroquinolin-4-yl benzoateCitraconic acid(11E,15Z)-9,10,13-trihydroxyoctadeca-11,15-dienoic acidTaurocholic acid sodium salt hydrateL-(+)-CitrullineMPHDesthiobiotinLysoPC 20:2Dl-3-Hydroxy-kynurenineMPK3-[(4-chlorobenzyl)thio]-4-methyl-5-undecyl-4H-1,2,4-triazole3-(3,4-dimethoxyphenyl)-1-(2-hydroxy-4,6-dimethoxyphenyl)propan-1-oneD-PanthenolL-beta-Imidazolelactic acidGDP16-Hydroxyhexadecanoic acidUR-144 N-(2-hydroxypentyl) metaboliteStearamideTyramine4,7-diphenyl[1,10]phenanthrolineCYM-5442(6E)-7-(2H-1,3-benzodioxol-5-yl)-1-(piperidin-1-yl)hept-6-en-1-oneNervonic acid(¡À)8-HEPE3b,7b-Dihydroxy-5-androsten-17-oneSphingosine (d18:1)KMHMGMG (18:2)(-)-CamphorSedanolideGly-ValDeoxyadenosine5,6-dimethoxy-2-(2-methoxyphenyl)-4H-chromen-4-oneN-(4-methoxyphenethyl)-N'-(2-methylphenyl)thioureaGatifloxacinAsp-Phe methyl esterLPE 17:2N4-AcetylcytidineS-Adenosyl-L-methionineLPE 17:0Azetidine-2-carboxylic acidCyclohexylsulfamateKNHRoyal jelly acidKNK3-(benzylthio)-5-[4-(tert-butyl)phenyl]-4-methyl-4H-1,2,4-triazoleThr-LeuCholine Glycerophosphate2-Aminobenzenesulfonic acidXanthurenic AcidCystamineAll-Trans-13,14-DihydroretinolDl-ThreitolCarbaprostacyclinIKKXanthineDiflorasone3-(3,4-Dihydroxyphenyl)-2-MethylalanineGemifloxacinL-GlutamineD-GlucosamineCholineDL-LeucineamideDinophysistoxin-1KPHN-ButylbenzenesulfonamideOctadecanamine(5E)-7-methylidene-10-oxo-4-(propan-2-yl)undec-5-enoic acidMonocrotalineKPKAcetylcysteineMethyl N-benzoylphenylalaninate5-HydroxymethyluracilL-Alanyl-L-prolinePC (16:2e/19:2)TBB4-(Diethylamino)salicylaldehydeInosine 5'-MonophosphateCarvoneKQHN-Acetyl-D-lactosamineAKB48 N-(4-hydroxypentyl) metabolite3-Amino-4-methylpentanoic acidIMK3-Methoxycinnamic acidHomo-Gamma-Linolenic Acid (C20:3)3-Methylindole2-Amino-1,3-octadecanediolS-Adenosylhomocysteinecyclohexyl{4-[4-nitro-2-(1H-pyrrol-1-yl)phenyl]piperazino}methanoneQuinidineDiosgeninLysopc 18:3LPE 15:03'-HydroxystanozololINK4-methyl-3-(methylthio)-5-undecyl-4H-1,2,4-triazole1,7,8-trihydroxy-3-methyl-1,2,3,4,7,12-hexahydrotetraphen-12-oneThromboxane B2L-Alanyl-L-Lysine5-Methyl-2'-deoxycytidine16¦Á-Hydroxydehydroepiandrosterone3-(3-morpholinopropyl)-2-(2-pyridinyl)-2,3-dihydro-4(1H)-quinazolinone2-(Dimethylamino)GuanosineKanosamine2-[(3S)-1-(3,4-Difluorobenzyl)-3-pyrrolidinyl]-1,3-benzothiazole3-hydroxy-2-(3-nitro-4-piperidinobenzyl)propanenitrileH-Gly-Pro-OHD-(+)-ProlineFeruloylcholineGKK3-hydroxy-2-[5-nitro-2-(1-pyrrolidinyl)benzyl]propanenitrileDL-Glutaminep-Coumaric acid3-Acetoxyurs-12-en-23-oic acidGlycochenodeoxycholic acid sodium salt1-(3,4-dimethylphenyl)-3-piperidinopyrrolidine-2,5-dionePhosphocholinepentane-1,2,3,4,5-pentol5-Methyl-dl-tryptophanLysopc 16:2 (2N Isomer)XylitolN-[(4-hydroxy-3-methoxyphenyl)methyl]-8-methylnonanamide1,2,10-Trimethoxy-5,6,6a,7-tetrahydro-4H-dibenzo[de,g]quinolin-9-ol(¡À)9-HpODEEstroneD-2-Aminoadipic acid2-Methoxyestradiol (2-MeOE2)alpha-FarneseneIPK1-morpholino-3-(4-nitrophenoxy)propan-2-olGLKRiboflavinL-(-)-alpha-Amino-epsilon-CaprolactamDeoxyinosine5¦Á-DihydrotestosteroneFeruloyl PutrescinePC (22:4e/18:5)2-mercapto-4,6-dimethylnicotinonitrilePC (14:1e/6:0)Milbemycin A4 oximeLenalidomide(+)-ar-TurmeroneBiotinFlunitrazepam-d3N2-Methylguanosine2-MethylbutyroylcarnitineMethyl beta-D-galactopyranosideBenzamidine Hydrochloride HydrateIRH1-(2-methoxy-4-nitrophenyl)pyrrolidine4,7,8-trimethoxyfuro[2,3-b]quinolineGNKN-(1-adamantyl)-N'-(2-chlorobenzyl)thioureaHexCer-NS (d18:2/18:2)PC (14:0e/2:0)THCCHEMBL1616521cis-4-Hydroxy-D-prolineVLHJWH 073 N-(4-hydroxybutyl)-d5Guanosine monophosphate(+/-)11(12)-DiHETVLK2-[(3S)-1-(2-Chlorobenzyl)-3-pyrrolidinyl]-5-methyl-1,3,4-oxadiazoleOleic acidEstriol2-[(3S)-1-Benzyl-3-pyrrolidinyl]-1,3-benzothiazole2-[(5-anilino-4-phenyl-4H-1,2,4-triazol-3-yl)thio]acetic acidLysopc 20:4EKKCytidine 5'-monophosphate (hydrate)5-S-cysteinyldopa3-Anilinoacraldehyde anil, HCl saltAmitriptyline-d311-Oxoetiocholanolone6-Phosphogluconic Acid Trisodium SaltVMKN-benzyl-2-(6-methoxy-2-naphthyl)propanamide1,2-di[3-(benzyloxy)benzylidene]hydrazine(+)-alpha-Lipoic acidLPE 14:0GPH4-Pyridoxic acidOctyl hydrogen phthalate5-[4-(phenylsulfonyl)phenyl]-1H-pyrazole2,4-dihydroxyheptadec-16-en-1-yl acetateglutathione disulfideGPKTyrosylalanineELKN-Acetyl-L-methionineAdenosine diphosphate (ADP)4-{4-[3-(4-chlorophenoxy)propyl]piperazino}-1H-indole5'-S-Methyl-5'-thioadenosineDL-LysineVNK(1E,4E)-1,5-bis(4-methoxyphenyl)penta-1,4-dien-3-onedUDP¦Á-Linolenoyl ethanolamideLysope 16:01,7-bis(4-hydroxyphenyl)-5-methoxyheptan-3-one7-(3,4-dihydroxyphenyl)-5-hydroxy-1-(4-hydroxyphenyl)heptan-3-oneBoc-beta-cyano-L-alanine2-furyl[4-(1H-indol-4-yl)piperazino]methanoneGQHEMK17¦Á-Hydroxyprogesterone3,4-dihydroxy-4-(4-methoxyphenyl)-1,2,3,4-tetrahydroquinolin-2-oneL-Fucose2-(4,4-diphenyl-1-piperidinobuta-1,3-dienyl)phenyl acetateAlanyltyrosine2-(1-benzofuran-2-yl)-4H-chromen-4-oneTKK1-MethylguanineGRHFmoc-L-IsoleucineD-Gluconic acid2,4-dichloro-N-{2-[(2-furylmethyl)amino]-1-methyl-2-oxoethyl}benzamideOcfentanil-d5ENKDulcitolProlinecGMPAngiotensin IVU-44069¦´-L-Glutamyl-L-glutamic acid2-[(3S)-1-(3,4-Difluorobenzyl)-3-pyrrolidinyl]-1,3-benzoxazoleVPHPuromycinTLHTLKFasciculic acid BThiamine1,2-dihydroxyheptadec-16-yn-4-yl acetateVQHTMKFumaric acidL-TyrosineArgininosuccinic acidCyclohexaneacetic acid(-)-Caryophyllene oxideEPKPC (19:2/18:5)Phe-Pro3-hydroxy-3-methylpentanedioic acid5-Phenylvaleric Acidgamma-GlutamyltyrosineGlycylprolineN-(5-acetamidopentyl)acetamideTNKL-Canavanine5-(2,4-difluorophenyl)-2-furaldehyde oximeNorverapamilmethyl 3,4,5-trihydroxycyclohex-1-ene-1-carboxylate3-(4-methylpiperazino)-5,6-diphenyl-1,2,4-triazineSpermidineS-(Methyl)Glutathione2-amino-8-mercapto-9H-purin-6-olLPC 18:3EQHfarensyl diphosphateErgosterol peroxide2-(tert-butyl)-1,3-thiazolane-4-carboxylic acid4-phenoxyphenyl 4-hydroxypiperidine-1-carboxylateSpectinomycinMeperidine-d5RauwolscineAvocadyne 1-acetateHydroxyglutaric acidPropionylcarnitineRKK4-decyl-3-hydroxy-5-oxooxolane-2,3-dicarboxylic acidERHUreidoisobutyric Acidp-Mentha-1,3,8-triene3-amino-2-phenyl-2H-pyrazolo[4,3-c]pyridine-4,6-diol3-Acetyl-11-keto-¦Â-boswellic acid4-methoxy-6-[2-(4-methoxyphenyl)ethyl]-2H-pyran-2-one2-(benzylthio)-1-phenethyl-5-(trifluoromethyl)-1H-benzo[d]imidazoleJNJ-1661010N-Acetyl-¦Á-D-glucosamine5-(tert-butyl)-2-methyl-N-(4-nitrophenyl)-3-furamidePyridoxal 5'-phosphateTPHRLHRLK2,6-Dihydroxybenzoic acidAcetophenone2-[2-oxo-2-(2-pyridylamino)ethoxy]acetic acidN-Acetyl-aspartic acidOcthilinonedimethyl 2-(3-nitro-2-pyridyl)malonatePC (17:2/22:6)ConiferinAcetyl-L-carnitineTQHPyridoxineRMHRMK3-(4-morpholinophenyl)-2-(4-nitrophenyl)acrylonitrile2-Benzyl-5-[(3S)-1-cyclobutyl-3-pyrrolidinyl]-1,3,4-oxadiazoleGlycohyocholic acid Sodium salt2-Amino-1,3,4-octadecanetriolALKStiripentolN-[2-chloro-6-(trifluoromethoxy)phenyl]-2,2-dimethylpropanamideLinoleoyl ethanolamideLDGTS 18:2Bilirubin9-Oxo-10(E),12(E)-octadecadienoic acidRNHRNKTenofovir1-O-(3,4,5-Trimethoxybenzoyl)-beta-L-galactopyranosemesityl(piperidin-4-yl)methanone hydrochlorideBetaineDGMG (18:2)LevodopaAMKAL 8810 Methyl ester4-Acetamidobutanoic acid6-AcetylmorphineCytidineEnrofloxacin6-Methylflavone2-amino-7,9-dimethyl-5-oxo-5H-chromeno[2,3-b]pyridine-3-carbonitrileIsoquinolineoctadec-9-ynoic acid2-(4-aminophenoxy)isophthalonitrilePyridostigmineP-Aminohippuric AcidANHANKN-BenzylformamideCys-GlyNAT13-331713_POS4-{2-[(2-chlorophenoxy)methyl]-1,3-thiazol-4-yl}benzonitrile¦Ø-Hydroxynorfentanyl7-methyl-3-nitroimidazo[1,2-a]pyridineBz-RS-ISer(3-Ph)-Ome1-MethylhistidinePLH5-(2,5-dihydroxyhexyl)oxolan-2-oneDeoxycorticosterone 21-glucosidePC (14:1e/2:0)N-{4-[(2R,3R)-3-(Hydroxymethyl)-5-oxo-2-morpholinyl]phenyl}acetamide2-Thio-acetyl MAGE¦Á-Lapachone4-acetyl-4-(ethoxycarbonyl)heptanedioic acidN'-[6-(tert-butyl)thieno[3,2-d]pyrimidin-4-yl]-4-methylbenzohydrazideDL-2-(acetylamino)-3-phenylpropanoic acidRQH5'-Deoxy-5'-(Methylthio)Adenosine1-PalmitoylglycerolPMKMonooleincyclohexyl[4-(2-methoxyphenyl)piperidino]methanoneAPHdUMPAPKAnthranilic acid3-(3,4-dihydroxyphenyl)propanoic acidProlylleucineAllantoinN1,N3-di(2-methylphenyl)-2-phenylmalonamide2-furaldehyde N-(1-phenyl-1H-pyrazolo[3,4-d]pyrimidin-4-yl)hydrazonePNKArachidonoyl serinol5-(6-hydroxy-6-methyloctyl)-2,5-dihydrofuran-2-one5-Hydroxytryptophan9-KODEAtropineL-SaccharopineNoradrenaline3-Indoleacrylic acidLysoPC 12:1MethylmalonateCytidine 5'-diphosphocholineLeukotriene B4 EthanolamideL-ErgothioneineMuscone3-Methoxy prostaglandin F1¦ÁTaurochenodeoxycholic Acid (sodium salt)MethionineMuramic acidPhosphopyruvic acidN-lactoyl-phenylalanineMAG (18:3)NKK1-[(3S)-3-(1,3-Benzoxazol-2-yl)-1-pyrrolidinyl]-3-methoxy-1-propanoneStyreneARH4-oxo-4-(5,6,7,8-tetrahydronaphthalen-1-ylamino)but-2-enoic acid1H-indol-3-yl(pyridin-2-yl)methanolPC (16:0e/2:0)N-gamma-Acetyl-N-2-Formyl-5-MethoxykynurenamineD-threo-Isocitric acidL-Histidinol2,6-Di-tert-butyl-1,4-benzoquinoneCarnosinePPKL-Glutathione oxidized1-Stearoylglyceroltrans-4-Hydroxy-L-proline4-Butylresorcinol3-Methyladipic acidUridine 5'-Diphospho-N-AcetylgalactosamineN-allyl-2-(2-methyl-1-benzofuran-3-yl)acetamideGlu-GlnLPC 15:0Diaminopimelic acidN,N-DiethyldodecanamidePQHN-Stearoyl taurinetrans-4-Phenyl-4-piperidinocyclohexanolNMKN2-(2,4-dimethylphenyl)-1,3-benzothiazol-2-amineJWH-018 N-(3-methylbutyl) isomerLeucylprolineAKB48 N-(4-fluorobenzyl) analogGamma-Aminobutyric acidNor-9-carboxy-¦Ä9-THCAla-IlePC (16:2e/2:0)PC (20:5e/17:2)NNKPhytosphingosine6,15-diketo-13,14-dihydro Prostaglandin F1¦Á3-(1-naphthylmethylidene)indolin-2-oneUR-144 N-(5-Chloropentyl) analogFisetin(¡À)5(6)-DiHETELysoPC 10:0DL-CarnitineHomoarginineErgocalciferolQuinineLKKCoumarinN-Desmethylclomipramine7-(2-hydroxypropan-2-yl)-1,4a-dimethyl-decahydronaphthalen-1-olPDMPNicotinate ribonucleosideSpermine1-(4-Methoxyphenyl)-2-propanoneHypoxanthine4-(2-((4-Methoxyphenyl)amino)oxazol-5-yl)benzonitrilePalmitoyl ethanolamideHomoserine2-Phenylpropionic acidN-Acetyl-DL-phenylalaninemorpholine-4-carboximidamide hydrobromideHippuric acidHeptadecanoic AcidNPKLLK15-Deoxy-¦¤12,14-prostaglandin J2-2-glycerol ester8-Isoprostaglandin F1¦ÁShikonin8-Isoprostaglandin F1¦ÂN-glycyl-L-prolineMethyl palmitateN6,N6,N6-Trimethyl-L-lysine5-Hydroxyindole5-MethyluridineLPC 17:2LMHAdenine(5-methyl-3-isoxazolyl)[4-(5-propyl-2-pyrimidinyl)piperazino]methanoneGlu-Val-Phe3-Nitro-L-TyrosineD-Proline(R)-PrunasinN1-(1H-indol-4-yl)cyclohexane-1-carboxamideInosineDl-3-HydroxynorvalineNaratriptanGlycerophospho-N-palmitoyl ethanolamineLNHLNKTetranor-12(S)-HETE5-[4-(tert-butyl)phenyl]-1,3,4-oxadiazole-2-thiolVitamin B12Arachidonic acid1,7-bis(4-hydroxyphenyl)heptan-3-oneN-Oleoyl GlycinePantethinePhenylpropiolic acid(2R)-2-[(2R,5S)-5-[(2S)-2-hydroxybutyl]oxolan-2-yl]propanoic acidAla-Gln2-(cyclopropylcarbonyl)-3-(4-fluoroanilino)acrylonitrilePB-22 N-(4-Hydroxypentyl)-3-carboxyindole metabolite2-[1-(4-isobutylphenyl)ethyl]-5-(3-nitrophenyl)-1,3,4-oxadiazolegamma-Glutamylcysteine6-Ketoprostaglandin F1¦Á6-phenyl-1,2,3,4-tetrahydro-2,5-benzodiazocin-1-oneN-PhenylacetylglutamineN-AcetylhistamineNicotinamide11(Z),14(Z),17(Z)-Eicosatrienoic acidMethyl dihydrojasmonate3-Methyl-2-oxobutanoic acid13-HPODEEstradiol BenzoateLPH7-Ketodeoxycholic acidH-gamma-glutamyl-glutamine6-HydroxymelatoninOleoyl ethanolamideN-(4-butyl-2-methylphenyl)-N'-[4-(4-methylpiperazino)phenyl]ureaD-GlucarateN7-MethylguanosinePyroglutamic acidPyridoxamine4-methoxyphenyl 3,5-dimethyl-1-phenyl-1H-pyrazole-4-carboxylatePhenothiazineL-Pyroglutamic acid9-Oxo-ODELQHPyridoxal 5-phosphate monohydratePC (5:0/13:1)Androsterone¦Â-Nicotinamide mononucleotidePC (17:0/18:3)3-[(4-hydroxyphenyl)methyl]-octahydropyrrolo[1,2-a]pyrazine-1,4-dioneUR-144 N-(5-Methylhexyl) analogLPE 18:02-methoxy-4-{3-[(4-methylbenzoyl)amino]butyl}phenyl 4-methylbenzoateLPE 18:2Beta-Muricholic acidAcetylcholineLPE 18:35-OxoETEN1-perhydrocyclopenta[d]pyrimidin-2-ylidenbenzene-1-sulfonamide1-[4-(1-adamantyl)phenoxy]-3-piperidinopropan-2-ol hydrochlorideN1-(3,4-dimethoxyphenethyl)-4-methylbenzene-1-sulfonamideDiphenylaminePantetheineVal-Serhydroxyphenylpyruvate6-phenyl-3,4-dihydro-1H-2,5-benzoxazocin-1-oneYLHLysopa 18:0YLKNicotinamide N-oxideUDP2-[(3-methyl-1-phenyl-1H-pyrazol-5-yl)amino]benzoic acidHKK7¦Á-HydroxytestosteronelipoamideLinolelaidic Acid (C18:2N6T)3'-Adenosine monophosphate (3'-AMP)N-[4-(6-methyl-1,3-benzothiazol-2-yl)phenyl]benzamide2,6-di(tert-butyl)-4-[2-(4-chlorophenyl)-1,3-thiazol-4-yl]phenolNicotinic Acid4-(methylthio)-6-phenyl-2-(3-pyridyl)pyrimidine-5-carbonitriletrans-CinnamaldehydeNoroxymorphonecis-gondoic acid13,14-dihydro-15-keto-tetranor Prostaglandin E2(5-L-Glutamyl)-L-Amino AcidPhe-PheN-Desmethyl sildenafilMag (18:1)JervineMethionine sulfoxideZaleplon-d4HLKDesalkylflurazepam-d4HexadecanamideGly-Tyracridine-9(10H)-thioneTauro-alpha-Muricholic acid sodium salt3-morpholino-5,6-diphenylpyridazine-4-carbonitrileYNK1,3-DimethyluracilHexCer-NS (d19:2/18:1)4-[3-(4-phenoxyphenyl)-1H-pyrazol-5-yl]morpholine4-[1-(acetyloxy)prop-2-en-1-yl]-2-methoxyphenyl 2-methylpropanoate16(R)-HETE3-N-Methyl-L-histidineN-Acetyl-L-leucineImidazoleacetic acid2,5-Furandicarboxylic acid5beta-Androstane-3,17-dione2'-DeoxyguanosineValylprolineWKK(S)-2-HydroxybutanoicacidGamithromycinLPE 16:1falseDeletion of Gliotoxin Biosynthesis Remodels the Intra- and Extracellular Metabolic Status of Aspergillus fumigatus, Alters Its Virulence-Related Phenotypes, and Modulates Host Immune Responses_Mycelium<p>Gliotoxin is a secondary metabolite produced by&nbsp;Aspergillus fumigatus, known to play a significant role in immune regulation and virulence phenotypes. This study investigates how the deletion of gliotoxin biosynthesis remodels the metabolic state of&nbsp;A. fumigatus&nbsp;and subsequently affects host immune responses. We employed a multi-omics approach, integrating fungal metabolomics and host transcriptomics data, to assess the impact of gliotoxin deletion on intra- and extracellular metabolic processes. The results showed that the loss of gliotoxin biosynthesis significantly altered the metabolic profile of&nbsp;A. fumigatus, with distinct changes observed in metabolites from both fungal mycelia and culture supernatants. These metabolic alterations were closely linked to changes in host cellular immune-related pathways. Notably, in macrophages and epithelial cells, treatment with gliotoxin led to significant changes in immune gene expression, revealing its role in modulating host immune responses.</p><p>Furthermore, treating macrophages with gliotoxin inhibited the secretion of inflammatory cytokines and reduced the cells' phagocytic capacity for&nbsp;A. fumigatus&nbsp;conidia, indicating that gliotoxin may play a role in suppressing host defense mechanisms. However, transcriptomic analysis also suggested that exposure to gliotoxin could promote the expression of certain immune genes, pointing to a complex interaction between the fungal metabolite and the host immune system. Through integrative analysis, we identified key metabolic and immune pathways affected by gliotoxin. These findings provide new insights into the impact of gliotoxin deletion on fungal virulence and host immunity, and offer a theoretical basis for potential therapeutic strategies in managing&nbsp;Aspergillus fumigatus&nbsp;infections.</p>2026-05-112026-03-09MTBLS14012Com_3_negCom_13_negCom_23_negCom_32_negCom_39_negCom_40_negCom_42_negCom_44_negCom_59_negCom_81_negCom_93_negCom_110_negCom_157_negCom_267_negCom_286_negCom_316_negCom_333_negCom_348_negCom_363_negCom_366_negCom_397_negCom_436_negCom_489_negCom_515_negCom_555_negCom_734_negCom_1021_negCom_1354_negCom_1481_negCom_1502_negCom_1516_negCom_1721_negCom_1866_negCom_1878_negCom_2835_negCom_2862_negCom_3018_negCom_3599_negCom_4445_negCom_4934_negCom_5766_negCom_8509_negCom_72_negCom_46_negCom_268_negCom_153_negCom_63_negCom_149_negCom_3990_negCom_119_negCom_523_negCom_266_negCom_99_negCom_520_negCom_773_negCom_78_negCom_4844_negCom_3313_negCom_8490_negCom_2795_negCom_974_negCom_2139_negCom_2762_negCom_452_negCom_5_negCom_7_negCom_20_negCom_24_negCom_31_negCom_41_negCom_43_negCom_65_negCom_86_negCom_89_negCom_94_negCom_97_negCom_127_negCom_137_negCom_148_negCom_166_negCom_185_negCom_205_negCom_207_negCom_211_negCom_236_negCom_249_negCom_253_negCom_275_negCom_281_negCom_282_negCom_297_negCom_302_negCom_325_negCom_354_negCom_360_negCom_392_negCom_404_negCom_411_negCom_461_negCom_476_negCom_479_negCom_485_negCom_511_negCom_524_negCom_540_negCom_542_negCom_590_negCom_592_negCom_657_negCom_675_negCom_682_negCom_692_negCom_712_negCom_715_negCom_722_negCom_735_negCom_746_negCom_772_negCom_774_negCom_776_negCom_789_negCom_805_negCom_846_negCom_911_negCom_918_negCom_946_negCom_969_negCom_972_negCom_977_negCom_981_negCom_986_negCom_993_negCom_994_negCom_1056_negCom_1065_negCom_1066_negCom_1090_negCom_1099_negCom_1119_negCom_1121_negCom_1135_negCom_1137_negCom_1149_negCom_1164_negCom_1181_negCom_1195_negCom_1241_negCom_1254_negCom_1291_negCom_1292_negCom_1337_negCom_1377_negCom_1386_negCom_1388_negCom_1406_negCom_1418_negCom_1419_negCom_1459_negCom_1504_negCom_1526_negCom_1539_negCom_1571_negCom_1629_negCom_1630_negCom_1635_negCom_1650_negCom_1711_negCom_1712_negCom_1739_negCom_1746_negCom_1770_negCom_1802_negCom_1820_negCom_1833_negCom_1891_negCom_1911_negCom_1939_negCom_1984_negCom_1991_negCom_2000_negCom_2009_negCom_2010_negCom_2064_negCom_2092_negCom_2104_negCom_2138_negCom_2162_negCom_2169_negCom_2210_negCom_2215_negCom_2226_negCom_2258_negCom_2275_negCom_2289_negCom_2308_negCom_2322_negCom_2346_negCom_2351_negCom_2370_negCom_2398_negCom_2413_negCom_2438_negCom_2451_negCom_2471_negCom_2474_negCom_2483_negCom_2533_negCom_2562_negCom_2571_negCom_2604_negCom_2619_negCom_2623_negCom_2647_negCom_2694_negCom_2721_negCom_2735_negCom_2751_negCom_2806_negCom_2901_negCom_2934_negCom_2947_negCom_2970_negCom_3021_negCom_3052_negCom_3159_negCom_3224_negCom_3265_negCom_3269_negCom_3281_negCom_3291_negCom_3305_negCom_3309_negCom_3423_negCom_3439_negCom_3477_negCom_3550_negCom_3591_negCom_3718_negCom_3758_negCom_3802_negCom_3865_negCom_3971_negCom_4056_negCom_4084_negCom_4131_negCom_4225_negCom_4415_negCom_4465_negCom_4503_negCom_4542_negCom_4600_negCom_4711_negCom_4722_negCom_4742_negCom_4827_negCom_4851_negCom_4854_negCom_4881_negCom_4888_negCom_4896_negCom_4922_negCom_4953_negCom_4962_negCom_4964_negCom_5034_negCom_5036_negCom_5146_negCom_5165_negCom_5183_negCom_5211_negCom_5269_negCom_5298_negCom_5358_negCom_5493_negCom_5584_negCom_5707_negCom_5736_negCom_5747_negCom_5784_negCom_5891_negCom_5901_negCom_5919_negCom_6036_negCom_6040_negCom_6059_negCom_6091_negCom_6112_negCom_6158_negCom_6162_negCom_6172_negCom_6174_negCom_6177_negCom_6294_negCom_6346_negCom_6359_negCom_6369_negCom_6420_negCom_6598_negCom_6694_negCom_6757_negCom_6908_negCom_6931_negCom_6957_negCom_7056_negCom_7093_negCom_7156_negCom_7272_negCom_7309_negCom_7319_negCom_7361_negCom_7379_negCom_7391_negCom_7438_negCom_7461_negCom_7550_negCom_7567_negCom_7617_negCom_7647_negCom_7740_negCom_7765_negCom_7794_negCom_7888_negCom_7906_negCom_7915_negCom_7933_negCom_7954_negCom_8010_negCom_8078_negCom_8129_negCom_8162_negCom_8211_negCom_8234_negCom_8292_negCom_8330_negCom_8398_negCom_8442_negCom_8546_negCom_8641_negCom_8783_negCom_8786_negCom_8794_negCom_8886_negCom_8929_negCom_9011_negCom_9076_negCom_9174_negCom_9188_negCom_9269_negCom_9307_negCom_9315_negCom_9372_negCom_9390_negCom_9528_negCom_9561_negCom_9570_negCom_9573_negCom_9826_negCom_10049_negCom_10190_negCom_10250_negCom_10256_negCom_10380_negCom_10430_negCom_10454_negCom_10570_negCom_10640_negCom_11017_negCom_11173_negCom_11292_negCom_11323_negCom_11380_negCom_11467_negCom_11568_negCom_11602_negCom_11680_negCom_11723_negCom_11999_negCom_12147_negCom_12152_negCom_12293_negCom_12361_negCom_12388_negCom_12463_negCom_12533_negCom_12596_negCom_12800_negCom_12985_negCom_13096_negCom_13179_negCom_13369_negCom_13392_negCom_13439_negCom_13593_negCom_13727_negCom_13777_negCom_13969_negCom_14004_negCom_14059_negCom_14098_negCom_14143_negCom_14220_negCom_14258_negCom_14640_negCom_14796_negCom_14830_negCom_14915_negCom_15134_negCom_15219_negCom_15396_negCom_15570_negCom_15762_negCom_15781_negCom_15926_negCom_16116_negCom_16169_negCom_16252_negCom_16266_negCom_16270_negCom_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