Quantitation was performed using external calibration curves based on the peak area ratio of each SCFA to the internal standard.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - reverse-phase"],"chromatography_protocol":["Chromatographic separation used helium carrier gas (1.0 mL/min) with the following temperature program: 100°C (1 min), increased to 180°C at 8°C/min, and held for 1 min.
"],"publication":["Targeted metabolomics of SCFAs in Caenorhabditis elegans."],"submitter_affiliation":["Ocean University of China"],"submitter_name":["jiayuan Cao"],"organism_part":["Whole Organism"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The worms were mixed with 50 µL of 15% phosphoric acid, 100 µL of internal standard (isohexanoic acid, 125 µg/mL), and 400 µL of diethyl ether.
"],"organism":["blank"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14034"],"author":["Liu yisuo.","Yi huaxi. Ocean University of China. yihx@ouc.edu.cn.","Cao jiayuan."],"data_transformation_protocol":["After tested for the series concentrations of standard solution, the calibration curve
was constructed by using the concentration of standard solution as the abscissa, and the
ratio of peak area between it and internal standard as the ordinate. The obtained linear
regression equation of each substance was shown in Table 1 (correlation coefficient
R >0.99).
"],"study_factor":["Probiotics","Caenorhabitis elegans","Targeted metabolome"],"submitter_email":["jiayuanalice@163.com"],"sample_collection_protocol":["After 5 days of incubation, nematodes from different treatment groups were collected.
"],"omics_type":["Metabolomics"],"study_design":["Aging","targeted metabolite profiling","microbiome"],"curator_keywords":["Aging","targeted metabolite profiling","microbiome"],"mass_spectrometry_protocol":["After vortexing and centrifugation, the supernatant was analyzed on a Thermo ScientificTM Trace 1310 GC-ISQ LT MS system (Waltham, MA, USA) equipped with Agilent DB-FFAP capillary column (30 m × 0.25 mm × 0.25 μm).
"],"metabolite_name":["Valeric acid","Isobutyric acid","Isovaleric acid","Propionic acid","Acetic acid","Butyric acid"],"additional_accession":[]},"is_claimable":false,"name":"Targeted Metabolome of Caenorhabditis elegans","description":"The effect of Lactiplantibacillus plantarum Q7 on SCFAs in Caenorhabditis elegans
","dates":{"publication":"2026-07-20","submission":"2026-03-12"},"accession":"MTBLS14034","cross_references":{"HMDB":["HMDB"]}}