MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/m_MTBLS14034_LC-MS_alternating_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/a_MTBLS14034_LC-MS_alternating_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/s_MTBLS14034.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_3.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_1.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_4.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_5.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/Q7_2.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_6.zipftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034/FILES/OP50_4.zipprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14034<p>Quantitation was performed using external calibration curves based on the peak area ratio of each SCFA to the internal standard.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - reverse-phase<p>Chromatographic separation used helium carrier gas (1.0 mL/min) with the following temperature program: 100°C (1 min), increased to 180°C at 8°C/min, and held for 1 min.</p>Targeted metabolomics of SCFAs in Caenorhabditis elegans.Ocean University of Chinajiayuan CaoWhole Organismmass spectrometry assay<p>The worms were mixed with 50 µL of 15% phosphoric acid, 100 µL of internal standard (isohexanoic acid, 125 µg/mL), and 400 µL of diethyl ether.</p>blankhttps://www.ebi.ac.uk/metabolights/MTBLS14034Liu yisuo.Yi huaxi. Ocean University of China. yihx@ouc.edu.cn.Cao jiayuan.<p>After tested for the series concentrations of standard solution, the calibration curve </p><p>was constructed by using the concentration of standard solution as the abscissa, and the </p><p>ratio of peak area between it and internal standard as the ordinate. The obtained linear </p><p>regression equation of each substance was shown in Table 1 (correlation coefficient </p><p>R >0.99).</p>ProbioticsCaenorhabitis elegansTargeted metabolomejiayuanalice@163.com<p>After 5 days of incubation, nematodes from different treatment groups were collected.</p>MetabolomicsAgingtargeted metabolite profilingmicrobiomeAgingtargeted metabolite profilingmicrobiome<p>After vortexing and centrifugation, the supernatant was analyzed on a Thermo ScientificTM Trace 1310 GC-ISQ LT MS system (Waltham, MA, USA) equipped with Agilent DB-FFAP capillary column (30 m × 0.25 mm × 0.25 μm).</p>Valeric acidIsobutyric acidIsovaleric acidPropionic acidAcetic acidButyric acidfalseTargeted Metabolome of Caenorhabditis elegans<p>The effect of Lactiplantibacillus plantarum Q7 on SCFAs in Caenorhabditis elegans</p>2026-07-202026-03-12MTBLS14034HMDB