Database search: HMDB, KEGG, LipidMaps (±10 ppm); MS/MS match:
MassBank, GNPS (cosine >0.7); RT tolerance ±30 s. Levels 1-4
identification. Software: MetaboAnalyst, SIMCA.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Chromatographic separation was performed on a Waters ACQUITY UPLC I-Class
System with an ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 × 100 mm). The
mobile phase consisted of (A) 0.1% formic acid in water and (B) 0.1%
formic acid in acetonitrile, with a gradient of 5-95% B over 22 min.
The column temperature was 40°C, flow rate 0.3 mL/min, and injection
volume 5 μL. The autosampler was maintained at 4°C.
"],"publication":["Non-targeted metabolomics of mouse feces in response to dietary fiber."],"submitter_affiliation":["yangzhou university"],"submitter_name":["wei liu"],"organism_part":["intestine"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Frozen fecal samples (~50 mg) were extracted with methanol/water (4:1)
containing internal standards, homogenized by bead-beating, sonicated,
and centrifuged. Supernatants were dried under nitrogen and reconstituted
in 100 μL mobile phase, with pooled QC samples and solvent blanks prepared
for quality control.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14046"],"author":["xiao xia. Yangzhou University. xiaoxia@yzu.edu.cn.","wei liu. Yangzhou University. lw15264770396@163.com."],"data_transformation_protocol":["Raw .raw files were converted to .mzML format using ProteoWizard
MSConvert (v3.0). Peak detection and alignment were performed with
XCMS (v3.14) using CentWave algorithm (10 ppm, peak width 5-20 s).
Features with RSD >30% in QC samples or detected in <50% of samples
were removed. Missing values were imputed by k-nearest neighbors
(k=10). Data were normalized by total ion current (TIC) or
probabilistic quotient normalization (PQN).
"],"study_factor":["Group"],"submitter_email":["lw15264770396@163.com"],"sample_collection_protocol":["Fecal samples were collected from C57BL/6J mice at 8 weeks of age. Samples were collected directly into sterile tubes and immediately snap-frozen in liquid nitrogen. All samples were stored at -80°C until further processing.
"],"omics_type":["Metabolomics"],"study_design":["fecal samples","Non-targeted metabolomic","dietary fiber"],"curator_keywords":["fecal samples","Non-targeted metabolomic","dietary fiber"],"mass_spectrometry_protocol":["Mass spectrometry was performed on a Thermo Q Exactive HF with HESI-II
source in positive and negative modes (separate runs). Spray voltage was
+3.5 kV (pos) and -3.2 kV (neg); capillary temperature 320°C; probe
heater 350°C; sheath/aux gas 35/10 arb. Mass range m/z 70-1050;
resolution 70,000 (MS) and 17,500 (MS/MS); ddMS2 on top 10 ions with
stepped NCE 20/40/60 eV.
"],"additional_accession":[]},"is_claimable":false,"name":"Non-targeted metabolomics of mouse feces in response to dietary fiber","description":"Non-targeted metabolomic analysis of fecal samples from mice with and without soy dietary fiber in their diet
","dates":{"publication":"2026-03-13","submission":"2026-03-13"},"accession":"MTBLS14046","cross_references":{}}