MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/m_MTBLS14067_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/m_MTBLS14067_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/a_MTBLS14067_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/a_MTBLS14067_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067/s_MTBLS14067.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14067Metabolite identification was performed by matching the mass spectrometry data (accurate mass and MS/MS fragmentation patterns) against public databases including the Human Metabolome Database (HMDB, http://www.hmdb.ca/), Metlin (https://metlin.scripps.edu/), and the in-house Majorbio database. The data processing and identification steps were integrated within the Progenesis QI software workflow.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseChromatographic separation was performed on an HSS T3 column (100 mm × 2.1 mm i.d., 1.8 µm). The column temperature was maintained at 40°C. The flow rate was 0.40 mL/min. The mobile phase A was water/acetonitrile (95/5, v/v) with 0.1% formic acid. Mobile phase B was acetonitrile/isopropanol/water (47.5/47.5/5, v/v/v) with 0.1% formic acid. The injection volume was 3 µL.Royal jelly and 10-hydroxy-2-decenoic acid mitigate alcoholic fatty liver disease in mice via the gut-microbiota-metabolite axis.Beijing Forestry University,lining renfecesmass spectrometry assay50 mg of fecal sample was placed in a 2 mL centrifuge tube with one 6 mm grinding bead. 400 µL of extraction solvent (methanol: water = 4:1, v/v) containing internal standards (including 0.02 mg/mL L-2-chlorophenylalanine) was added. The mixture was homogenized in a frozen tissue grinder at -10°C, 50 Hz for 6 min, followed by low-temperature ultrasonic extraction at 5°C, 40 kHz for 30 min. The sample was then incubated at -20°C for 30 min and centrifuged at 4°C, 13000 g for 15 min. The supernatant was transferred to an autosampler vial with an insert for LC-MS analysis. A pooled quality control (QC) sample was prepared by mixing equal volumes of supernatant from all samples. QC samples were injected every 5-10 sample runs to monitor the repeatability of the analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14067Guochen Liu. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. 19863935773@163.com.Huimin Zhang. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. 1324342085@qq.com.Yuhang Xiao. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. xyuhang163@163.com.Xinyu Ma. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. 13209155447@163.com.Weiman Gao. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. gaoweiman225@bjfu.edu.cn.Lining Ren. The (primary) affiliation, institution or organisation for this contact. e.g. European Bioinformatics Institute. Beijing Forestry University, Beijing 100083, China. renlining525@bjfu.edu.cn.Aidong Sun. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. adsun@bjfu.edu.cn.Shijing Jiang. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. College of Biological Science and Technology, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China. 18501268988@163.com.The raw LC-MS data were imported into Progenesis QI software (Waters Corporation, Milford, USA) for preprocessing. The workflow included baseline filtering, peak picking, integration, retention time correction, and alignment. The resulting data matrix containing retention time, m/z, and peak intensities was then exported for further analysis.Treatmentrenlining525@bjfu.edu.cn1. Animal Treatment and Fecal Sample CollectionMale C57BL/6J mice (8 weeks old, 20 ± 2 g) were obtained from Sibeifu Biotechnology Co., Ltd. (License number: SCXK (Beijing) 2024-0001). All mice were housed in specific pathogen-free (SPF) facilities with controlled temperature and humidity (21–25°C, 12-hour light/dark cycle) and had free access to food and water. After one week of acclimatization, the mice were randomly allocated into seven groups (n = 6 per group). The alcoholic fatty liver disease (AFLD) model was established using chronic alcohol feeding combined with acute alcohol gavage. Mice in the control group (Con) received a Lieber-DeCarli (LD) liquid diet for 15 days. Mice in the other groups were gradually adapted to a 5% (v/v) ethanol LD liquid diet over 5 days and then maintained on this diet for an additional 10 days. On day 16, all ethanol-fed mice received a single oral gavage of 31.5% (v/v) ethanol (5 g/kg body weight), while the Con group received an isocaloric maltodextrin solution.Based on physiological and biochemical evaluations, four representative groups (n = 6 per group) were selected for untargeted metabolomics analysis: the control group (Con), the model group (Mol), the high-dose royal jelly group (H-RJ, 200 mg/kg/day), and the high-dose 10-HDA group (H-10-HDA, 100 mg/kg/day). Fecal samples were collected from each mouse under sterile conditions on day 15, strictly representing the period after the chronic feeding phase and exactly one day prior to the final acute alcohol gavage. Upon collection, the fresh fecal samples were immediately snap-frozen in liquid nitrogen and stored at −80°C until further analysis.MetabolomicsMulti-omics studyalcoholic fatty liver diseaseuntargeted metabolite profilingMulti-omics studyalcoholic fatty liver diseaseuntargeted metabolite profilingMass spectrometry analysis was performed on a Thermo UHPLC-Q Exactive HF-X system. Data were acquired in both positive and negative ion modes. The mass scan range was m/z 70-1050. The ion spray voltage was set to 3500 V for positive mode and -3000 V for negative mode. Sheath gas flow rate was 50 arb, auxiliary gas flow rate was 13 arb, and ion source heating temperature was 450°C. The stepped normalized collision energy (NCE) was set to 20-40-60 V.Bakkenolide IiiaPe(18:1/0:0)Pbd DimerCholestane-3,7,12,25-Tetrol-3-GlucuronideL-RhamnuloseCellopentaosePhe-IleCys Phe SerMethylarbutinL-HistidineZaragozic Acid ADeoxycholic AcidCoumarinic AcidTriglyceride Palmitate5-Acetamido-1,4-Anhydro-2,5-Dideoxy-2-[(4-Methoxybenzyl)Amino]-D-ArabinitolErucoylacetoneCastasteroneErythrinasinate ASerylisoleucine3Beta,7Alpha-Dihydroxy-5-CholestenoateDl-4-Hydroxyphenyllactic AcidAcetyl-Arginyl-Glycyl-Aspartyl-SerinamideCarboprost6-Hydroxyhexanoic AcidAsebotoxinPenfluridolKinetensin 4-7Dg(31:0)Val-Pro-Pro20-Carboxy-Leukotriene B4GailiapentaTeucvidin6-Acetyl-2,3-Dihydro-2-(Hydroxymethyl)-4(1H)-PyridinonePolysorbate 20Silybin4-[N-(P-Coumaroyl)Serotonin-4''-Yl]-N-FeruloylserotoninSativanIndole Carboxylic Acid Sulfate8-Aha-CampGamma-GlutamyltyrosineTetrahydrocorticosteronePgp(5-Iso Pgf2Vi/16:1(9Z))MaltopentaoseHophe-Lys-OhOryzalide BCis-3-Hexenyl LactatePenicillic AcidLeu-Val2-Hydroxyquinoline-3-Carboxylic Acid7-Deoxyechinosporin8-Amino-7-Oxononanoic AcidNeopetasitenineD-Aspartic AcidSpiculisporic AcidN-AcetyllactosamineVal-Pro-AsnAsparaginylisoleucineRiddelliine N-OxideCrustecdysonePressinoic Acid25-AcetylvulgarosideGlyceryl Monolinoleate2-[4-(2-Hydroxy-3-Methylbut-3-Enoxy)Phenyl]Acetic AcidGly-IleMometasoneNonacosanoylcarnitine4-Hydroxynonenal Glutathione2,4-QuinolinediolAla-AlaMomordicilin2-((4-Chlorophenyl)Thio)-N-(4-(Pyridin-2-Yl)Thiazol-2-Yl)AcetamideCryptomeridiolHomogentisic Acid3-Oxocholic AcidCarbidopaIsovalerylcarnitineEgonol Glucoside(3R)-3-Hydroxy-N-[(3S)-2-Oxooxolan-3-Yl]ButanamideN1-[(2,6-Dimethylmorpholino)Carbonyl]-3-Chloro-4-Fluorobenzamide2-(14,15-Epoxyeicosatrienoyl) GlycerolCoclaurine2,3,4,5-Tetrahydroxy-6-(1,2,3,4-Tetrahydroxybutyl)Oxane-2-CarbaldehydeN-(2,4-Dimethoxyphenyl)-5-Phenyl[1,2,4]Triazolo[1,5-A]Pyrimidin-7-AmineDl-AcetylcarnitineD-Gal Alpha 1->6D-Gal Alpha 1->6D-Glucose(1R)-1-Amino-2-Sulfanylethanesulfinic Acid11-Hydroxyeicosatetraenoate Glyceryl EsterBeta-CortolProstaglandin B2Stachyoside AGentiobiosideAmibegronL-Phenylalanine(S,E)-Zearalenone8,11-Eicosadiynoic AcidArgatrobanCoumaryl AcetateGlaucarubolone 15-O-Beta-D-Glucopyranoside2-Hydroxyphenylacetic Acid SulfateEcgonine13,14-Dihydro-15-Keto-20-Ethyl Prostaglandin F2Alpha8-Hydroxy-5,6-Octadienoic Acid(E)-4-[(1-Methoxy-1-Oxopropan-2-Yl)Amino]-4-Oxobut-2-Enoic AcidPe(18:2/0:0)Uridine, 2'-Deoxy-2'-Fluoro-2'-Methyl-, (2'R)-Ile-IleTrelanserinMusabalbisiane A2-Cyano-3-(3,4-Dimethoxyphenyl)Acrylic AcidSquamoxinoneLibenzapril5Z-7-OxozeaenolN-MalonyltryptophanZeatinPe(18:0/0:0)2-Dodecylbenzenesulfonic Acid4-O-(Indole-3-Acetyl)-D-Glucopyranose3-Hydroxycinnamic AcidArg Gly AspMelibiitol5-Chloro-2'-DeoxyuridinePterosin G1H-Pyrazolo[3,4-D]Pyrimidin-4-Amine6-GingerolPseudo-AnisatinVinyl-L-NioNivalenolN-Acetyl-L-Tyrosine5-Hydroxy-P-Mentha-6,8-Dien-2-OnePrednisoloneMethionine SulfoneIndole-3-CarboxaldehydeGlu-ValXanthosineDimethyl-3-Hydroxypyrid-4-One(+/-)12,13-DihomeDodecyl BenzenesulfonateMetabolite M62-Propenamide, 2-Cyano-3-(4-Hydroxy-3,5-Bis(1-Methylethyl)Phenyl)-Ile-Ile-SerSuberic AcidFoliandrinLeu Val GlyVerbascosideS-Cysteinosuccinic AcidZolmitriptanThymine DimerFasciculic Acid B2'-DeoxyuridineVal Ala Ser AspStachyose(3Beta,22R,23R,24S)-3,22,23-Trihydroxystigmastan-6-One6-(2-Amino-2-Carboxyethyl)-4-HydroxybenzothiazoleIle-Val10-Hydroxydecanoic Acid5-(10-Nonadecenyl)ResorcinolPoncirinHydroquinone3-Hydroxy-1-(4-Hydroxy-2-Methoxyphenyl)-3-(4-Methoxyphenyl)Propan-1-OneN-Palmitoyl PhenylalanineTrihydroxycoprostanoic AcidSa-Beta-Gal3-Hydroxy-7-Methoxy-3H-2-Benzofuran-1-OneGibepyrone DGlucose PropionateAcetyl Citrate4-Amino-1-(6-Aminopurin-9-Yl)Pyrimidin-2-OneProstaglandin-C2D-(+)-Malic Acid1,1,1-Trifluoroheptadecan-2-OnePhytyl Acetate(6E)-8-HydroxygeraniolXenognosin A3-Hydroxylup-20(29)-Ene-23,27,28-Trioic AcidProstaglandin J2N-Butyrylhomoserine LactoneBarrinic AcidZierin4-[(1E,3E)-Hepta-1,3-Dienyl]-3-(Hydroxymethyl)Cyclohexane-1,2-DiolPhysostigmineStercobilinN-CyclohexanecarbonyltetradecylamineN-Carboxyethyl-Gamma-Aminobutyric AcidNeoacrimarine K2-[(3S)-1-(3,4-Difluorobenzyl)-3-Pyrrolidinyl]-1,3-BenzoxazoleS-(3-Oxo-3-Carboxy-N-Propyl)CysteineChlorowax 40Calcidiol6-Hydroxymusizin 8-O-B-D-Glucopyranoside5-(4-Carboxybutylperoxy)Pentanoic Acid3-Methylorsellinic AcidMyo-Inositol GlutamateD-ApioseCoenzyme Q2Methyl 3-[(4-Anilino-2-Methoxyphenyl)Sulfamoyl]Thiophene-2-CarboxylateTaurine4-(3-Amino-2-Hydroxypropoxy)PhenylacetamidePe(16:1/0:0)FruleuileSoybean Lecithin15H-11,12-Eeta2-HydroxyethanesulfonatePiperonyl ButoxideRicinoleic AcidMeconineO-Benzoyl-L-Malic AcidMedicagenateAndrographolideProscillaridin[12]-GingerdiolMethyl-[12]-GingerdiolGly-Pro-Gly-Arg-Ala-PhePyriminobac-MethylN2-Ethyl-2'-Deoxyguanosine8-Hydroxyguanosine2-Hydroxyadipic AcidDiosmetin-7-O-Beta-D-GlucopyranosideUrsinic AcidCarbamoyl (2R)-2,5-DiaminopentanoateDemissidine5,8-Dihydroxy-3-(4-Hydroxybenzyl)-7-Methoxy-4-Chromanone 8-Acetate6-Hydroxynicotinic AcidCitbrasine7-HydroxymitragynineSulbactamUzarigenin3B-Hydroxy-5-Cholenoic AcidFraxidin11B-HydroxyprogesteroneAmpicilloylSoyasapogenol DIsopropylmaleic AcidAlternariolVal-Ile(E)-3,10-Dihydroxy-12-Methoxy-4,9-Dimethyl-12-Oxododec-6-Enoic AcidLeukotriene C4Ethyl Hydrogen Sulfate2',3',4'-TrihydroxyacetophenoneRuscogenin3'-Hydroxy-5,7,4',5'-Tetramethoxyflavone5-Methoxy-3-(1,2,3,6-Tetrahydropyridin-4-Yl)-1H-IndoleEtamucineN-Arachidonoyl Glutamic AcidDopamineDopamine 4-SulfateNeohesperidoseDodecyl GallateCyclooctylureaPe(15:0/0:0)Soyasapogenol ATestosteroneGamma-L-Glutamyl-Gamma-L-Glutamyl-L-Methionine4-Ene-Valproic Acid7-Chloro-4-Piperidinoquinoline2-Thiouracil1-Isopropyl-1,2,3,4,-Tetrahydro-Beta-Carboline-3-Carboxylic AcidFructose CitrateHalometasone2-(1-{2-Oxo-2-[(2-Pyridinylmethyl)Amino]Ethyl}Cyclohexyl)Acetic AcidNalfurafineEnterodiol Glucuronide{1-[2-(4-Carbamimidoyl-Benzoylamino)-Propionyl]-Piperidin-4-Yloxy}-Acetic Acid2,4-Dihydroxy-2H-1,4-Benzoxazin-3(4H)-OneLactodifucotetraoseTyrosine-TyrosinateIndacaterol-8-O-Glucuronide9,11-Methane-Epoxy Pgf1Alpha19-Oxotestosterone2-Amino-5-Chloro-3-Phenyl-3H-Indol-3-OlGoshuyic AcidMomordol12,20-Dihete6B-Hydroxy-8A-Methoxy-7(11)-Eremophilen-12,8-OlideTriflumizoleLucidenic Acid M6,8-Dihydroxy-3-(10-Hydroxyundecyl)-3,4-Dihydro-1H-Isochromen-1-OneFlusilazoleIsoscopoletinBrassinolideTetranorprostanedioic Acid9R,10S-EpomeOctadeca-2,4-Dienedioic Acid24,24-Difluoro-1Alpha,25-Dihydroxyvitamin D31-(Cyclohexylmethyl)Indazole-3-Carboxylic AcidDigoxigeninD-Leucic Acid11'-Carboxy-Gamma-ChromanolSec-O-GlucosylhamaudolLovastatin Acid1-(4-O-Beta-D-Glucopyranosyl-3-Methoxyphenyl)-3,5-DihydroxydecaneBetavulgarin GlucosideDiacetoxyscirpenolAlpha-Methyl-M-Tyrosine2-Hydroxy-L-Methionine2-Hexadec-7-Enylicosa-8,11-Dienedioic AcidMethyl 5-[(5-Phenylthieno[2,3-D]Pyrimidin-4-Yl)Oxy]-2-FuroateTyr-Hophe-Oh3-Aminosalicylic Acid4,6,8-Trihydroxy-3-Methyl-3,4-Dihydroisochromen-1-OneLignicolArabinosylhypoxanthine4-O-Carboxymethyl AscochlorinGlycochenodeoxycholic Acid 3-GlucuronideSulfolithocholylglycineP-Hydroxynefazodone20-Cooh Ltb4N-AcetylaspartateOligomycinMollicellin CN6-Benzyl-7H-Purine-2,6-DiamineMethyl 4-Amino-5-Ethyl-3-Thiophenecarboxylate6''-O-Alpha-D-GalactopyranosylciceritolP-Salicylic AcidCaninDihydro-3-Coumaric AcidPangamic AcidTrans-Piceid7-Methyladenine6-Methyl 2-Galloylgalactarate3,9-Dimethoxy-6,11A-Dihydro-[1]Benzofuro[3,2-C]Chromen-6A-OlDesoxycarbadoxOctenoyl-L-CarnitineTyramine4-(Cytidine 5'-Diphospho)-2-C-Methyl-D-Erythritol4-Methylumbelliferyl Beta-D-XylopyranosideGly-ValN-Acetyl-D-Tryptophan5,9-Epidioxy-3-Hydroxyergost-7-En-6-OneKanakugiolDg(Pgj2/I-15:0/0:0)MelezitoseSterculic AcidPeonidin 3-(6''-P-Coumaroyl-Glucoside) 5-GlucosideValtrateL-Gulonolactone1-DehydroperuvinineAmafolone10-Hydroxy-2E-Decenoic AcidMethyl 3,8-Dihydroxy-9-Oxoxanthene-1-Carboxylate2-(3-(2-Aminoethyl)-1H-Indol-5-Yloxy)Acetyl-L-Tyrosyl-GlycinamideUrothionSucrose 1',4'-(4,4'-Dihydroxy-3,3'-Dimethoxy-B-Truxinate)Al 8810N-Methoxysuccinyl-Ala-Ala-Pro-Val3-O-Beta-D-Galactopyranosyl-L-ArabinoseNizatidine5-(4-Hydroxypentyl)Benzene-1,3-DiolResolvin E1Xanthurenic AcidHymenoxonMirificinFrangulanineNotoginsenoside R10(2S,3S,4S,5R)-3,4,5-Trihydroxy-6-Pyren-1-Yloxyoxane-2-Carboxylic AcidGlycogen3-Hydroxy-5-(2-Hydroxypropyl)-2-Methyl-2,3-Dihydropyran-6-OneXanthine13-Hydroxyoctadeca-2,4-Dienoic Acid6-Ketoprostaglandin E1[2-Acetyl-4-(1,2-Dihydroxypropyl)Phenyl] 1,3-Benzodioxole-5-CarboxylateMaslinic Acid1B,3A,7A-Trihydroxy-5B-Cholanoic Acid1,2-DiferuloylgentiobioseN-(1-Propylbenzimidazol-2-Yl)Quinoline-2-Carboxamide(7Z)-Tetradec-7-Enedioylcarnitine2-Methylidene-3-Oxo-4A,7,8,8A-Tetrahydro-4H-1,4-Benzoxazine-7-Carboxylic AcidSodium Benzofuran IsophthalateAsteltoxinRosavinLasepitinLeucylphenylalanineMethyl (3B,11X)-3-Hydroxy-8-Oxo-6-Eremophilen-12-OateHistidinyl-AsparagineActeosideImazamethabenz-Methyl5-(10,13-Nonadecadienyl)-1,3-Benzenediol3,4-Dhpea-Ea2-[(Acridin-9-Ylamino)Methyl]-4-[Bis(2-Chloroethyl)Amino]Butanoic AcidLeu Ile GlyCore OligosaccharideQuinic Acid2-(10-Heptadecenyl)-6-Hydroxybenzoic AcidDolicholide2-Aminopurine6-Hydroxy-4-Methoxy-3-(3-Methyl-2-Butenyl)-2-(2-Phenylethenyl)Benzoic AcidAllyl Hexanoate20, 22-DihydrodigoxigeninPropane-1,3-Diyl Bis(4-Aminobenzoate)Methyl 2-(Aminosulfonyl)BenzoateAndrostane-3,17-Diol 17-Sulfate2-Methylidene-4-(Oxiran-2-Yl)Butanoic AcidS-AdenosylhomocysteineLacto-N-Biose I3,4-Dimethyl-5-Pentyl-2-Furanpentadecanoic Acid(1-Methyl-2-Oxoquinolin-4-Yl) N,N-Dimethylcarbamate3-Dehydroteasterone5-Hydroxy-N-FormylkynurenineN-Linoleoyl PhenylalanineNordeoxycholic Acid2-Methoxyacetaminophen Sulfate[5-(2-Thienyl)-3-Isoxazolyl]MethanolGeniposidic AcidBlumenol C O-[Rhamnosyl-(1->6)-Glucoside]Orotic AcidKaempferol 3-(2''-Rhamnosyl-6''-Acetylgalactoside) 7-RhamnosideN-Methyl-D-Aspartic Acid7,4'-Dihydroxy-8-Methylflavan(+/-)13-Hpode2-Isopropyl CitrateGlacin A2-Stearyl CitrateEdulisin IvCis-SolaminPantoyllactone GlucosidePyrrhoxanthinolAzelaic AcidTizoxanide GlucuronideTaurocholic Acid 3-SulfateMaltohexaoseGemeprost3-DeoxyguanosineHemateinTws119Beta-AmanitinIsopropyl Tartaric Acid2,5-Dioxopyrrolidin-1-Yl 2-(Acetylthio)AcetateEthyl Beta-D-GlucopyranosideBolandiolDelta-HydroxylysylnorleucinePelargonidin 5-GalactosideFenspirideTheasapogenol AEchinacosideHydroxymethylbilaneLotrafibanDeoxyinosinePheophorbide ASpermine NonoateDesmethyl DabrafenibGlucose Pyruvate LactateDibutyl SulfosuccinateCaffeic AcidCurcumin DiglucosideArabinogalactoseIndole-3-Acetic Acid1,2-Dipalmitoyl-Rac-Glycero-3-PhosphoethanolamineNevadensinTyrosylhydroxyproline3,9-Dimethoxypterocarpan12'-Apo-B-Carotene-3,12'-Diol3,14-Dihydro-15-Keto-Tetranor Prostaglandin E2EvogliptinBeta-D-Xylopyranosyl-(1->5)-Alpha-L-Arabinofuranosyl-(1->5)-L-ArabinoseNarcissin1,3,2-Dioxathiolane 2,2-DioxideFumagillin7-Methyl-Cholic AcidFructosamine3-Hydroxy-1,2-Dimethylpyridin-4(1H)-OneVanillic Acid22-Deoxocucurbitacin DAsitrilobin D3-Keto Fusidic Acid(+/-)-Tryptophan2-HydroxyquinolineDodec-6-EnedioylcarnitineVerbascoside A5,5-Diphenylbarbituric AcidMepiprazoleDl-2-Hydroxy Stearic Acid6-Hydroxymusizin 8-O-GlucosideS-(9-Hydroxy-Pga1)-GlutathioneTris(2-Chloroethyl) PhosphateRotenone3-Hydroxy-4-Methyl-2-(2-Methylbutanoyloxy)Pentanoic AcidN-Acetyl-D-PhenylalanineMonic Acid2-Keto-3-Deoxy-D-Gluconic Acid6-HydroxymelleinKaempferol 3-O-Beta-Robinoside 7-O-Alpha-L-RhamnopyranosideThiaclopridDihydroferulic Acid 4-O-SulfateMonoketocholic AcidJgb1741Guanosine6-(I(2)-D-Glucopyranosyloxy)-4-Methoxy-5-Benzofuranpropanoic Acid5-S-Glutathionyl-Aminochrome ReducedDihydrocortisolKetoace1-[(4-Hydroxycyclohexyl)Methyl]Indazole-3-Carboxylic AcidDimethylaminoparthenolide2-(Carboxymethoxy)-4-Methoxybenzoic Acid3Alpha,7Alpha,12Alpha-Trihydroxy-5Beta-CholestanateN-(Carbomethoxyacetyl)-4-S-Chlorotryptophan(Z)-2-(4-Methylpent-3-Enyl)-6-Oxohept-2-Enoic AcidDemethylsuberosinHelenalin4-(4-Chlorophenyl)-1-Phenyl-1,3-Dihydro-2H-Imidazole-2-ThioneSesaminol Glucosyl-(1->2)-[Glucosyl-(1->6)]-Glucoside1-O-Feruloyl-Beta-D-GlucoseEriojaposide BLiquiritin ApiosideDihydrophaseic AcidAmphibine HSalmefamolVanillic Acid 4-O-Sulfate12-Ketodeoxycholic Acid2-Hydroxy-6-[(Z)-Nonadec-8-Enyl]Benzoic AcidArg-Val-TyrStreptozocinLys-Tyrme-OhBorapetoside7-Hydroxy-3-(Hydroxymethyl)-5-Methylisochromen-1-OnePyraclostrobin1-Methyl-5-(4-Benzoyl)Pyrrole-2-Acetic Acid 2-(Theophylline-7-Yl)Ethyl EsterSojagolMethyl (3X,10R)-Dihydroxy-11-Dodecene-6,8-Diynoate 10-GlucosideDihydroresveratrol 3-Glucuronide2-Ethyl-N-{4-[(2R,3R)-3-(Hydroxymethyl)-5-Oxo-2-Morpholinyl]Phenyl}Butanamide6,8-Dihydroxy-7-Methoxy-3-Methyl-3,4-Dihydroisochromen-1-One5-Acetamidovalerate2-Octenedioic AcidNorethindrone Oxime1,10-Epoxy-3,8-Dihydroxy-4,11(13)-Germacradien-12,6-OlideSaxitoxinPe(17:0/0:0)Methyl Dioxindole-3-AcetateFucosyllactose7Alpha,12Alpha,26-Trihydroxycholest-4-En-3-OneSakuranin3'-BenzoylsalicinOleyl AcetateS-(1-Propenyl)-Cysteine Sulfoxide(R)-HeraclenolCarbamoyl Cholesterol1-DeoxynojirimycinLabdadien-15,16-Olid-19-Oic AcidPhe-Gln-Lys4-[(E)-5-Hydroxy-3-Methylpent-3-Enyl]-3,5,5-Trimethylcyclohex-2-En-1-One4-Tert-Butylphenyl Glycidyl EtherSuccinic Acid, Sulfo-, 1,4-Dioctyl Ester1-O-Cinnamoyl-Beta-D-GentiobioseAmyloseIsofraxidinAlpha-D-Gal-(1->3)-Beta-D-Gal-(1->4)-D-GlcnacLeu-AlaEpiphyllic AcidScytalonePgg-GlucanSenkyunolide GPe(14:0/0:0)(2R)-2-Acetamido-5-Hydroxy-2-(Sulfanylmethyl)Pentanoic AcidSialosyl-Tn SaccharideSinistrinAspartic Acid Methyl Ester2,2-Dimethylpropanal 2,4-Dinitrophenylhydrazone2-(Arabinosylamino)-3-(Glucosylamino)PropanenitrileObscurolide A1(-)-Epigallocatechin 3-CinnamateIsoglobotriaosePectolinarin4-NitrophenolEncecalin3'-Deoxy-3'-FluorothymidineGlucoliquiritin ApiosideIndoline-2-Carboxylic Acid(2R)-2-Acetamido-3-[[(2R)-2-Acetamido-2-Carboxyethyl]Disulfanyl]Propanoic AcidN-Acetyl-L-AlanineTetramethylscutellareinBiotin SulfoxideDimethylsphingosineAcetyl-Dl-LeucineN-Lauroyl Serine3-Hydroxypentadecanoic Acid9H-Purin-9-AmineLicheninAjugose2-Ethoxy-6(5-Tetrazolyl)XanthoneDifructose Anhydride Iii2-[2-(3-Nitropyridin-2-Yl)Sulfanylphenyl]-1,4,5,6-TetrahydropyrimidineGenistein 4'-O-GlucosideFerrostatin-1Sedoheptulose 1-PhosphateSacubitrilatDioctyl PhthalateGlyasperin C1-Ipomeanol2-Methyl-3-Pyrimidin-2-Yl-Propionic AcidExo,Exo-1,8-Epoxy-P-Menthane-2,6-DiolYucalexin P15(4E,7E)-Dodeca-4,7-Dienedioic AcidPiperdialLeu-LeuN-Methyl-L-ProlineGibberellin A8-CatabolitePhe Pro Lys8-HydroxyadenineMitoxantroneN-Undecylbenzenesulfonic AcidErythroskyrinMarmesin Rutinoside6-Methyl-7-Nitro-2,3-Dihydro-1,4-Benzodioxine3A,7A,12A-Trihydroxy-5B-Cholestan-26-Al4-AcetylzearalenoneMesaconitineNiazidin2-(Malonylamino)Benzoic AcidTyramine-O-SulfateNeoagarotetraoseMesobilirubinogenSialyl Lewis X Antigen(+/-)-Ethylketocyclazocine(R)-CanadineClindamycin Phosphate6-(2-Hydroxyethoxy)-6-Oxohexanoic AcidDocosanal1,5,6,7-Tetrahydro-4H-Indol-4-OneAloeresin BAlectrolVal-LeuSulindac6-Keto-28-Homobrassinolide11-Oxo-Androsterone Glucuronide3,4-Methylenedioxybenzoic AcidDehydroepiandrosterone Sulfate4-[(1-Cyclopropylethyl)Anilino]-4-Oxobutanoic Acid3-Hydroxy-4-ButanolideInosine Pyruvate1-Methoxyindole-3-Carboxylic AcidMetoprololAmylopectinHistidinohydroxylysinonorleucine22-Hydroxydocosanoic Acid5-Hydroxy DiclofenacAceneuramic Acid3-[4-(Sulfooxy)Phenyl]Propanoic AcidEthyl 3-OxohexanoateSinensetin1-Methyluric AcidCulmorinDichlorpropEthisteroneAllantoin(R)-2-Hydroxysterculic AcidGlutamyltyrosine3-MethoxynobiletinKavainAcexamic AcidLoxoribineIsoswertisin 4'-RhamnosideFusarin C6,8-Dihydroxy-3-(2-Hydroxy-4-Oxopentyl)Isochromen-1-OneN-Formylmethionine6,7-Dimethyl-8-(1-D-Ribityl)LumazineAndrosinKaempferol 3-(2'''-(E)-Caffeylsophoroside)-7-GlucosidePi-1032,8-QuinolinediolEsmololGly-Leu2-Methylcyclohexan-1,3-DioneVomifoliol 9-[Xylosyl-(1->6)-Glucoside]Cohibin ACortisone Acetate3-Epipapyriferic Acid[8]-DehydrogingerdioneL-PyridosineAsn-His-TyrSarmentogenin2-Hydroxyhexadecanoic AcidHmboa Dihexose3-(3-Pyridyl)-1-PropanolGigantol2,2,2-Trifluoro-N-Methyl-N-(Trifluoroacetyl)AcetamideThomsen-Friedenreich AntigenErgocornine5-(1,2-Dihydroxypropan-2-Yl)-4-Methylbicyclo[3.1.0]Hexan-2-OnePlicatic AcidIsorhamnetin 3-Sophoroside-7-RhamnosideAustalide I4-Ethylphenylsulfate2-Hydroxysebacic Acid3-(Glutathion-S-Yl)Acetaminophen5'-Hydroxy-3'-MethoxysativanZeranolFkgk 11ChalconaringeninPhe-LeuPanaxatriolCyclohexaamyloseA-L-Arabinofuranosyl-(1->3)-B-D-Xylopyranosyl-(1->4)-D-XyloseHydroxyphenyllactic AcidAla-IleMaltotetraose15(R)-Hydroperoxy-EpePropoxateBeta-N-AcetylglucosamineUndecylenic AcidMimosine4-Dodecylbenzenesulfonic Acid4-Phenylbutyl Glucosinolate16A-Hydroxyestrone2,3-Dihydroxypropyl OctanoateDelphinidin 3-GlucosideDocosahexaenoic Acid Ethyl EsterCyclo(Leu-Phe)Macrolactin-ADesmethyldeschlorobenzoyl IndomethacinIle-LeuCarbonyloxycamptothecinHydroxyaniline MustardMycophenolic AcidAdipic AcidRibavirin MonophosphateGlycerophosphoserineAcetyl-Dl-ValineSyrups, Hydrolyzed Starch, HydrogenatedKaempferol 3-O-Caffeoyl-Sophoroside 7-O-Glucoside3,4-Dihydroxy-2-Hydroxymethyl-1-PyrrolidinepropanamideAmarogentin1-[6-(2-Carboxypyrrolidin-1-Yl)-6-Oxohexanoyl]Pyrrolidine-2-Carboxylic AcidMethyl Nicotinate(2S)-3-Methyl-2-Naphthalen-1-Yloxybutanoic Acid3-Hydroxyphenylacetic AcidGlucosylisomaltol5-Hydroxyindol-2-Carboxylic AcidVaranic AcidRibostamycinCholesterol GlucuronideCholylmethionineCephalomannine8-DeoxylactucinForsythiasideCycluron(-)-Caryophyllene OxideGlutaminyltyrosineSulfametrole4-(3,4-Dimethoxyphenyl)-3-Methyl-1H-Pyrazol-5-AminePhosphoramidonGlu-IleBarnidipine13-Hydroxy-5'-O-MethylmelledonalVerbascose(3E,5E)-2-(4-Methoxy-4-Oxobutyl)Nona-3,5-Dienoic AcidAdavosertib1-O-Alpha-D-Glucopyranosyl-D-MannitolAla Pro LeuMab-Chminaca Metabolite M11InosineLysope(19:0)-D5Cinncassiol C1 19-GlucosideDidanosineLumichrome3-Isobutyl-1-MethylxanthineGanoderiol G9-Oxo-Nonanoic AcidPe(17:1/0:0)2-MaleylacetateIle-PheN,N-Dimethyl-N'-P-Tolylsulphamide7A-Hydroxy-5B-Cholestan-3-OneCentpropazineVal-Ile-SerEstrone SulfateMethotrexateProstaglandin F1A10-Hydroxymelleolide15-HexadecanolideCucurbitacin A3,5-Dimethoxybenzoic Acid20-Hydroxyeicosatetraenoic AcidThreonylisoleucinePa(Pgf2Alpha/8:0)1-Hexanol ArabinosylglucosideMaltotrioseN-Hexadecyl-L-HydroxyprolineTetrahydrodeoxycorticosteroneCd 17904-Hydroxy-6-[(E)-2-Hydroxy-7,9-Dimethyl-4-Oxoundec-7-Enyl]-3-Methylpyran-2-OneOxyglutinosone11-Beta-Hydroxyandrosterone-3-GlucuronideCyclo(Pro-Val)O-(17-Carboxyheptadecanoyl)CarnitineIndan-1-One 1-(4,5-Dihydro-1H-Imidazol-2-Yl)Hydrazone17A,20A-DihydroxycholesterolOridonin3-Cresotinic AcidRifamycinsIsohomovanillic AcidSalidroside12S-HhtAncymidolMethionine SulfoxideN-Oleoyl Phenylalanine13-HotreEcdysone3-(4-Nitrophenyl)Imidazo[1,5-A]PyridineN-Myristoyl ThreonineAsp-IleCappariloside BAmorfrutin AHomodolichosterone9-O-Acetylneuraminic AcidPanoseOvalicinGlutamylleucylarginine1-Palmitoyl-2-Linoleoyl-3-Acetyl-Rac-Glycerol4-Oxododecanedioic AcidN-Acetyl-L-Glutamic AcidLipoamide6''-O-Acetylglycitin1,4-Cyclohexanedicarboxylic Acid8-Acetoxypinoresinol 4-GlucosideEthyl 2-PyrrolecarboxylateCyclohexanecarboxylic Acid3,3'-Bisanigorufone3B-Hydroxy-6B-Methoxy-7(11)-Eremophilen-12,8A-OlideTranexamic Acid16-Oxo-PalmitatePregnanetrioloneLactitol1-ArachidonoylglycerolN-Formylanthranilic AcidSesaminol Glucosyl-(1->2)-GlucosideTaurine Lactate5-(4-Chlorophenyl)Sulfonyl-2-Methylpyrimidin-4-AmineNicotinic Acid4-Cholesten-7Alpha,12Alpha,24-Triol-3-OneUndecanedioic Acid3,12-Dihydroxy-13-Methoxypodocarpa-8,11,13-Trien-7-One4-Hydroxyproline GalactosideEnt-Prostaglandin E2Norcarfentanil3-Sn-PhosphatidylethanolamineRaffinoseCynaropicrinAscorbyl Palmitate3,5-Dihydroxy-2-(4-Hydroxyphenyl)-3,4-Dihydro-2H-Chromen-7-Yl PentofuranosideTripdiolide(8As)-7-(3-Phenylpropyl)Hexahydroimidazo[1,5-A]Pyrazin-3(2H)-One2,3-Dihydroxybenzoic AcidProstaglandin F3ADiethyl Hydrogen PhosphateAminofurantoin2-(4-Aminophenylthio) Acetic AcidDelta-CehcAlpha-Dimorphecolic AcidLeonoside BO-Arachidonoyl GlycidolClozapine4-Hydroxy-6,6,9A-Trimethyl-4,5,5A,7,8,9-Hexahydro-3H-Benzo[G][2]Benzofuran-1-One4-Hydroxy-3-Methoxy-Cinnamoylglycine3-Sulfopropyl Methacrylate(Z)-Resveratrol 3,4'-DiglucosideTyphasterolAdynerin3,4,5-Trimethoxycinnamic AcidLeonoside AfalseRoyal jelly and 10-hydroxy-2-decenoic acid mitigate alcoholic fatty liver disease in mice via the gut-microbiota-metabolite axisRoyal jelly (RJ) and its signature bioactive compound, 10-hydroxy-2-decenoic acid (10-HDA), are believed to possess hepatoprotective properties. However, their protective effects and underlying mechanisms against alcoholic fatty liver disease (AFLD) remain unclear. In the present study, we integrated 16S rRNA sequencing, untargeted metabolomics, and molecular biology analyses to evaluate their therapeutic potential in an AFLD mouse model. The results demonstrated that RJ and 10-HDA significantly improved alcohol-induced liver injury by reducing lipid accumulation and mitigating dyslipidemia, inflammation, and oxidative stress. The 16S rRNA sequencing revealed that RJ and 10-HDA ameliorated alcohol-induced gut microbiota disruption. In particular, supplementation with beneficial doses of RJ (200 mg/kg/day) and 10-HDA (100 mg/kg/day) reduced the abundance of Pseudomonadota and Escherichia, and increased the abundance of Verrucomicrobiota (e.g., Akkermansia) and Lactobacillus. Untargeted metabolomics further indicated that the host metabolic regulation involved key pathways such as serotonergic synapse, bile secretion, and tryptophan metabolism. Moreover, RJ and 10-HDA activated the adenosine monophosphate-activated protein kinase (AMPK) pathway, promoting fatty acid β-oxidation while suppressing lipogenesis. Notably, integrated correlation analysis revealed that the beneficial faecal metabolites restored by the intervention were closely associated with the activation of the hepatic AMPK pathway. In contrast, alcohol-enriched deleterious metabolites exhibited the opposite trend, linking luminal metabolic shifts to upstream hepatic lipid regulation. Collectively, these findings suggest that RJ and 10-HDA mitigate AFLD by modulating the gut-microbiota-metabolite axis and activating the AMPK signaling pathway, supporting their potential use as dietary supplements in strategies aimed at managing AFLD and promoting liver health.2026-05-142026-03-17MTBLS14067HMDB0001106HMDB0014672HMDB0002322HMDB0001414;PW_C001092HMDB0000716;PW_C000567HMDB0001257;PW_C000971HMDB0001256;PW_C000970HMDB0255248HMDB0034198HMDB0012234HMDB0252514HMDB0000062;PW_C000044HMDB0000641;PW_C000500HMDB0000086HMDB0002172HMDB0036458HMDB0012136HMDB0000148;PW_C000095HMDB0001280HMDB0000904;PW_C000723HMDB0248691HMDB0240464HMDB0002005HMDB0005923HMDB0256275HMDB0000064;PW_C000046HMDB0254398HMDB0000854HMDB0006029HMDB0252149HMDB0012497HMDB0000162;PW_C000106HMDB0033978HMDB0000247HMDB0000600HMDB0030091HMDB0061927HMDB0004290HMDB0062715HMDB0000468HMDB0006583HMDB0255618HMDB0015654HMDB0011668HMDB0000661HMDB0000132;PW_C000086HMDB0000721HMDB0028695HMDB0302754HMDB0004620HMDB0257791HMDB0039354HMDB0000060;LMFA01060003;PW_C000042HMDB0011727HMDB0242328HMDB0040154HMDB0003539HMDB0059611HMDB0033748HMDB0006591HMDB0037115HMDB0060665HMDB0246355HMDB0254202HMDB0000289HMDB0302439HMDB0000245HMDB0000897HMDB0015527HMDB0255553HMDB0030765HMDB0029423HMDB0000830HMDB0029898HMDB0000786HMDB0014444HMDB0011648HMDB0013815HMDB0041587HMDB0011718HMDB0002035HMDB0249714HMDB0035940HMDB0260240HMDB0035859HMDB0002039HMDB0254871HMDB0000740HMDB0061918;HMDB0244886HMDB0029196HMDB0003374HMDB0029128HMDB0240710HMDB0032914HMDB0034887HMDB0001545;PW_C001188HMDB0040202HMDB0015506HMDB0001200HMDB0252454HMDB0005765HMDB0030697HMDB0038676HMDB0060493HMDB0032332HMDB0000034;PW_C000024HMDB0001476;PW_C001137HMDB0038593HMDB0005199HMDB0000017;PW_C000011HMDB0006900HMDB0255152HMDB0029023HMDB0029114HMDB0259557HMDB0033932HMDB0249379HMDB0028736HMDB0011639HMDB0000157;PW_C000102HMDB0243923HMDB0029000HMDB0032985HMDB0304027HMDB0028720HMDB0006115HMDB0040153HMDB0253648HMDB0000714;HMDB0011723;HMDB0000714HMDB0255868HMDB0034367HMDB0001182HMDB0253941HMDB0259836HMDB0257371HMDB0303973HMDB0014789HMDB0258299HMDB0038890HMDB0000262;HMDB0010337;HMDB0000262;PW_C000179HMDB0028912HMDB0038085HMDB0034157HMDB0303782HMDB0248277HMDB0000210;HMDB0250782;PW_C000136HMDB0033002HMDB0029770HMDB0032590HMDB0247737HMDB0030150HMDB0001924;PW_C001283HMDB0014945HMDB0002759HMDB0006061HMDB0255981HMDB0003134HMDB0255300HMDB0258533HMDB0243496HMDB0012134HMDB0062175HMDB0060472HMDB0255495HMDB0000466HMDB0000734HMDB0006700HMDB0000929;PW_C000741HMDB0257133HMDB0011166HMDB0061822HMDB0015383HMDB0256028HMDB0035337HMDB0001104HMDB0060527HMDB0062645HMDB0245586HMDB0060752HMDB0028890HMDB0255966HMDB0014554HMDB0303660HMDB0004083HMDB0246883HMDB0029427HMDB0033092HMDB0254256HMDB0248358HMDB0254763HMDB0014917HMDB0303360HMDB0302362HMDB0000715;PW_C000566HMDB0003320HMDB0000832HMDB0247904HMDB0249463HMDB0252688HMDB0032936HMDB0302665HMDB0245956HMDB0259056HMDB0251053HMDB0243557HMDB0042008HMDB0040844HMDB0004076HMDB0245302HMDB0244887HMDB0002886HMDB0256544HMDB0304481HMDB0015168HMDB0256122HMDB0254094HMDB0248692HMDB0259752HMDB0242632HMDB0005771HMDB0013010HMDB0252495HMDB0303181HMDB0000763;PW_C000609HMDB0014476HMDB0002209HMDB0030664HMDB0000068;PW_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;PW_C009196HMDB0060990HMDB0000353HMDB0011179HMDB0251980HMDB0253834HMDB0001383HMDB0255737HMDB0006344HMDB0031620HMDB0036748HMDB0036296HMDB0013649HMDB0248517HMDB0015129HMDB0002596HMDB0015224HMDB0250404HMDB0002182HMDB0247936HMDB0242680HMDB0036627HMDB0303367HMDB0033468HMDB0033598HMDB0033783HMDB0029108HMDB0000277HMDB0036074HMDB0304293HMDB0000806HMDB0247386HMDB0011176HMDB0253367HMDB0030271HMDB0258787HMDB0031402HMDB0254813HMDB0030011HMDB0252556HMDB0000053;LMST02020007;PW_C000037HMDB0034695HMDB0254757HMDB0014748;HMDB0245769HMDB0242004HMDB0015282HMDB0062552HMDB0258508HMDB0037845HMDB0033823HMDB0031056HMDB0247832HMDB0034498HMDB0033690HMDB0029852HMDB0031929HMDB0250660HMDB0037530HMDB0242736HMDB0000949HMDB0030608HMDB0037417HMDB0251483HMDB0256205HMDB0031923HMDB0242379HMDB0012993HMDB0034362HMDB0253373HMDB0006294HMDB0242503HMDB0259765HMDB0014439HMDB0060044HMDB0253027HMDB0241929HMDB0006888HMDB0004610;HMDB0004628;HMDB0004610;LMSP01030001HMDB0000252;LMSP01010001;PW_C000172HMDB0038152HMDB0030008HMDB0012624HMDB0034146HMDB0034689HMDB0029638;HMDB0303310HMDB0030461HMDB0031821HMDB0254669HMDB0062410HMDB0013154HMDB0030482HMDB0041206HMDB0040284HMDB0000495HMDB0013654;LMFA08020197HMDB0243990HMDB0285504HMDB0041333HMDB0036850HMDB0032797HMDB0258802HMDB0248837HMDB0004586HMDB0302259HMDB0041371HMDB0030039HMDB0036579HMDB0006547HMDB0252515HMDB0253203HMDB0062534HMDB0037631HMDB0013627HMDB0041085HMDB0012458HMDB0000631;PW_C000491HMDB0035144HMDB0012215HMDB0035918HMDB0002214HMDB0029885HMDB0035834HMDB0035304HMDB0034424HMDB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