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used an internal tool called LipidOracle, which performs MS2 annotation by rule-based approaches and spectral libraries and MS1 annotation and RT checking. It harmonises nomenclature compliant with LIPIDMAPS.</p>MetaboLightsPublicLiquid Chromatography MS - positive - reverse-phase<p>LC was performed on a Agilent 1290 Infinity II with a BEH C18 column. Column temperature was 60°C, flow rate 1.2ml/min and the injection volume 2ul. The gradient was from 85% buffer A consisting of acetonitrile/H2O (6:4, v/v), with 0.1% formic acid and 10mM ammonium acetate to 99% buffer B consisting IPA/acetonitrile (9:1, v/v) with 0.1% formic acid and 10mM ammonium acetate after 2 minutes.</p>Untargeted lipidomics analysis of yeast metabolic proteins degradations to investigate the role of metabolic proteins in lipid metabolism.ETH ZürichStephan DurotsolventWhole Organismmass spectrometry assay<p>Strains were grown in triplicates to an OD of 0.5 with a volume of 1ml per replicate in 96 deep-well plates. Protein degradation was induced with 10uM 5-Ph-IAA. The cultures were then centrifuged, supernatant removed and the lipids extracted with 150ul MeOH/IPA 50:50 for 24h at -20°C. The samples were centrifuged and supernatant containing the lipids transferred to a fresh plate and stored at -20°C until measurement. Pooled study samples were created by pooling aliquots from 96 randomly picked samples. Blanks denote to solvent blanks.</p>blank sampleSaccharomyces cerevisiaehttps://www.ebi.ac.uk/metabolights/MTBLS14098Stephan Durot. ETH Zurich. stephan.durot@hotmail.com.Nicola Zamboni. ETH Zurich. zamboni@imsb.biol.ethz.ch.Maya Schuldiner. Weizmann Institute of Science. maya.schuldiner@weizmann.ac.il.Rosario Valenti. Weizmann Institute of Science. rosariom@weizmann.ac.il.<p>Raw files were converted to mzML files and a master matrix was generated with an internal software (MASSter). The resulting ion intensities were TIC normalized before downstream analysis.</p>AUXINstephan.durot@hotmail.com<p>704 strains of S. cerevisiae, with each strain having a specific protein with an AID (auxin-inducible degron) tag, grown in SD medium with histidine, methionine and uracil in a shaker at 30C and with 250rpm in 96 deep-well plates. Protein degradation was induced with 10uM 5-Ph-IAA for 10 minutes.</p>Metabolomicsliquid chromatography mass spectrometry assayLipidomicsprotein degradationliquid chromatography mass spectrometry assayLipidomicsprotein degradation<p>Mass spectrometry measurements were performed on a Sciex ZenoTOF 7600 equipped with an electrospray ionization source operating in positive mode. The source temperature was 500°C, spray voltage 5500V, curtain gas at 45, CAD gas at 7 and ion source gas 1 and 2 at 70. Mass detection was done from 70 to 2000m/z. Data acquisition was performed with Data Dependent Acquisition, with the collision energy at 30V, accumulation time in MS2 at 5ms and zeno threshold at 1000000.</p>falseUntargeted lipidomics analysis of yeast metabolic proteins degradations to investigate the role of metabolic proteins in lipid metabolismWe combine auxin-inducible degradation of 500 characterized and 204 uncharacterized proteins with untargeted lipidomics providing a quantitative readout of cellular lipidomic states.2026-03-242026-03-20MTBLS14098