MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/m_MTBLS14124_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/m_MTBLS14124_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/s_MTBLS14124.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/a_MTBLS14124_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124/a_MTBLS14124_LC-MS_negative_reverse-phase_metabolite_profiling.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14124<p>Metabolite annotation was performed based on accurate mass and MS/MS fragmentation information when available. Detected features were matched against reference metabolite databases for putative identification.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using an ultra-high-performance liquid chromatography system coupled to mass spectrometry for non-targeted metabolomics analysis. Metabolites were separated on a reversed-phase C18 column. Mobile phase A consisted of water containing 0.1 percent formic acid, and mobile phase B consisted of acetonitrile containing 0.1 percent formic acid.</p>Effects of Trichoderma and Salicylic Acid/Methyl Jasmonate on the Growth, Development, and Lipid Metabolism of Codonopsis pilosula.Lanzhou University of Technologypengfei GaoRootmass spectrometry assay<p>Fresh root samples were ground into a fine powder in liquid nitrogen before extraction. Approximately 50 mg of each sample was extracted with 1.0 mL of methanol/water solution. The mixture was vortexed thoroughly, sonicated for 30 min, and then centrifuged at 12000 rpm for 10 min at 4 C. The supernatant was collected and filtered through a 0.22 um membrane prior to LC-MS analysis. A pooled quality control sample was prepared by mixing equal aliquots from all samples.</p><p><br></p>Codonopsishttps://www.ebi.ac.uk/metabolights/MTBLS14124Wenbin Sun.Zhongduo Yang.Pengfei Gao. School of Life Science and Engineering, Lanzhou University of Technology No. 287, Langongping Road, Qilihe district, Lanzhou, Gansu. 1950063375@qq.com.Li Li.Yonggang Wang. Lanzhou University of Technology, Lanzhou University, Lanzhou, Gansu, China. wangyg@lut.edu.cn.Xiangfang Tang.Xinjian Wang.Ning Zhua.Lili Liu.Hong Zhang.Yuanlin Ma.Wen Luo.<p>Raw LC-MS data were processed for peak detection, retention time alignment, feature extraction, and normalization. Quality control based filtering was applied to reduce analytical variation, and the processed data matrix was used for downstream statistical analysis.</p>Treatment1950063375@qq.com<p>Fresh root samples of Codonopsis pilosula were collected for non-targeted metabolomics analysis. Root tissues were harvested from healthy plants. After collection, the samples were briefly cleaned to remove surface impurities, immediately frozen in liquid nitrogen, and stored at -80 C until metabolite extraction. Biological replicates were prepared for each group.</p>MetabolomicsSalicylic acidTrichodermaCodonopsis pilosulaCodonopsisMetabolic regulationJasmonic aciduntargeted metabolite profilingSalicylic acidTrichodermaCodonopsis pilosulaCodonopsisMetabolic regulationJasmonic aciduntargeted metabolite profiling<p>Mass spectrometry analysis was performed using a high-resolution mass spectrometer equipped with an electrospray ionization source. Data were acquired in both positive and negative ion modes for non-targeted metabolomics analysis. The mass scan range was set according to the experimental method used in this study. Source parameters including capillary voltage, source temperature, desolvation temperature, cone voltage, and gas flow were optimized according to the instrument method.</p>POS1POS3POS2POS803POS802POS801POS800POS807POS806POS805POS804POS809POS808POS810POS9POS8POS814POS5POS813POS4POS812POS7POS811POS6POS816POS815POS821POS820POS825POS824POS823POS822POS829POS828POS827POS832POS831POS830POS836POS835POS834POS833POS839POS838POS837POS169POS171POS170POS175POS174POS173POS172POS179POS178POS176POS182POS181POS180POS186POS185POS184POS189POS188POS187POS193POS192POS191POS197POS196POS194POS199POS198POS447POS205POS446POS687POS445POS203POS686POS444POS202POS208POS449POS207POS448POS692POS450POS691POS690POS696POS695POS453POS694POS452POS210POS693POS451POS458POS699POS698POS697POS455POS213POS219POS459POS217POS460POS465POS223POS464POS222POS221POS220POS469POS227POS468POS226POS225POS466POS224POS229POS228POS230POS471POS470POS476POS234POS233POS232POS231POS238POS479POS237POS478POS236POS477POS235POS239POS483POS241POS482POS240POS481POS480POS487POS486POS485POS484POS242POS249POS248POS489POS488POS246POS490POS252POS493POS251POS492POS491POS256POS255POS496POS254POS495POS259POS258POS499POS257POS260POS267POS264POS269POS270POS274POS273POS272POS271POS278POS277POS275POS279POS281POS280POS283POS289POS288POS287POS286POS601POS843POS600POS842POS841POS840POS605POS604POS846POS603POS845POS602POS844POS609POS608POS849POS848POS38POS39POS34POS35POS36POS850POS30POS32POS612POS854POS611POS853POS610POS852POS851POS616POS615POS614POS613POS48POS44POS45POS46POS47POS861POS40POS860POS41POS42POS623POS865POS622POS864POS621POS863POS620POS862POS50POS627POS869POS868POS625POS867POS624POS629POS628POS59POS55POS56POS57POS58POS630POS872POS51POS871POS52POS870POS53POS54POS634POS876POS875POS632POS874POS631POS873POS61POS638POS637POS879POS636POS878POS877POS639POS66POS67POS68POS69POS641POS883POS62POS640POS882POS881POS64POS880POS65POS645POS887POS644POS886POS402POS643POS885POS401POS642POS884POS649POS407POS648POS889POS405POS888POS404POS409POS408POS890POS652POS894POS651POS650POS656POS898POS414POS655POS897POS413POS654POS653POS418POS659POS658POS416POS657POS899POS415POS663POS421POS662POS420POS661POS660POS667POS425POS666POS424POS665POS423POS664POS422POS429POS669POS427POS668POS426POS19POS17POS18POS670POS12POS13POS14POS674POS432POS673POS672POS430POS671POS10POS678POS436POS677POS435POS676POS434POS675POS433POS439POS438POS679POS437POS26POS27POS28POS29POS681POS680POS24POS443POS684POS442POS200POS683POS441POS20POS682POS440POS21POS902POS901POS900POS906POS904POS903POS909POS908POS907POS913POS912POS911POS910POS917POS915POS914POS919POS920POS70POS71POS72POS923POS922POS921POS925POS929POS78POS73POS74POS75POS931POS80POS930POS81POS82POS935POS934POS933POS932POS939POS938POS937POS88POS89POS84POS85POS700POS942POS91POS941POS940POS945POS702POS944POS701POS943POS90POS708POS707POS949POS706POS947POS99POS95POS96POS97POS98POS711POS953POS710POS952POS951POS950POS715POS957POS714POS956POS713POS712POS954POS719POS718POS959POS960POS292POS290POS295POS299POS298POS297POS326POS567POS325POS324POS565POS323POS1071POS1070POS329POS1073POS328POS569POS1072POS327POS571POS1075POS570POS1077POS1076POS575POS1079POS333POS574POS1078POS332POS573POS331POS572POS330POS579POS337POS578POS336POS577POS335POS576POS1060POS1062POS339POS1061POS338POS582POS1064POS340POS581POS1063POS580POS1066POS1065POS586POS1068POS344POS102POS585POS1067POS343POS101POS584POS342POS1069POS348POS106POS589POS588POS346POS587POS103POS109POS1051POS108POS1050POS349POS593POS1053POS351POS592POS1052POS350POS591POS1055POS590POS1054POS1057POS355POS113POS1056POS112POS595POS1059POS353POS111POS1058POS359POS117POS358POS116POS599POS357POS115POS598POS356POS114POS1040POS119POS118POS1049POS1042POS362POS1041POS361POS1044POS360POS1043POS1046POS366POS124POS1045POS365POS123POS1048POS364POS122POS1047POS363POS121POS128POS369POS127POS368POS126POS367POS125POS129POS131POS372POS130POS371POS370POS135POS134POS375POS374POS380POS384POS383POS141POS382POS381POS388POS146POS145POS144POS143POS149POS1091POS148POS1090POS389POS147POS1092POS1095POS1094POS391POS1097POS395POS1096POS394POS393POS151POS392POS150POS399POS398POS397POS396POS1080POS159POS158POS1082POS1081POS1084POS1083POS160POS1086POS164POS1085POS163POS1088POS162POS1087POS161POS168POS1089POS167POS964POS721POS963POS720POS962POS961POS726POS968POS967POS724POS966POS723POS965POS729POS728POS727POS969POS971POS970POS733POS975POS732POS974POS731POS973POS730POS972POS979POS736POS978POS735POS977POS734POS976POS739POS738POS740POS982POS981POS980POS744POS986POS985POS742POS748POS506POS747POS989POS505POS746POS988POS504POS745POS987POS503POS749POS507POS751POS993POS750POS992POS991POS990POS755POS997POS754POS996POS512POS753POS995POS752POS994POS510POS759POS517POS758POS516POS757POS999POS515POS998POS514POS519POS518POS520POS760POS765POS523POS764POS522POS521POS528POS769POS527POS768POS767POS525POS529POS1039POS1038POS1031POS1030POS1033POS1032POS1035POS531POS1034POS530POS1037POS770POS1036POS777POS535POS776POS534POS533POS532POS539POS538POS537POS536POS1028POS1027POS1029POS780POS1020POS1022POS1021POS784POS1024POS542POS300POS783POS1023POS541POS782POS1026POS540POS781POS1025POS546POS304POS787POS545POS303POS786POS544POS302POS785POS543POS301POS549POS307POS548POS306POS789POS547POS305POS1017POS1016POS1019POS1018POS1011POS1010POS795POS1013POS311POS552POS1012POS310POS551POS1015POS550POS1014POS799POS798POS556POS797POS555POS796POS554POS319POS318POS559POS317POS558POS316POS1006POS1005POS1008POS1007POS1009POS560POS1000POS564POS1002POS322POS563POS1001POS321POS562POS1004POS561POS1003falseEffects of Trichoderma and Salicylic Acid/Methyl Jasmonate on the Growth, Development, and Lipid Metabolism of Codonopsis pilosula<p>Endophytic fungi and phytohormone signaling can jointly influence growth–defense trade-offs in medicinal plants by reprogramming metabolism in a stage-dependent manner. To elucidate how the endophytic fungus Trichoderma longibrachiatum&nbsp;FG and exogenous salicylic acid (SA) and methyl jasmonate (MJ) regulate Codonopsis pilosula&nbsp;(C. pilosula), seedlings were assigned to five treatments: control (CK), FG, SA, MJ, and SA + MJ (SM). Assessments were conducted at multiple growth stages using morphological measurements, photosynthetic and physiological assays, antioxidant profiling, and non-targeted metabolomics. All treatments promoted seedling growth, with SM producing the most pronounced improvements in biomass-related traits, including root growth and overall biomass accumulation. FG, SA, and MJ modified photosynthetic traits and redox status, as evidenced by changes in photosynthetic parameters, antioxidant enzyme activities, lipid peroxidation levels, and endogenous signaling molecules (SA, JA, NO), indicating coordinated regulation of primary physiology and stress-related responses. UHPLC/Q-TOF-based metabolomic profiling revealed temporally distinct patterns: FG induced limited early changes but triggered marked metabolic reprogramming at 50 days, whereas SA, MJ, and SM elicited stronger metabolite shifts at 15 days. Differential metabolites were predominantly lipids and lipid-like molecules, with steroid and steroid-derived pathways emerging as key responsive hubs; notably, FG at the late stage was associated with enrichment of intermediates linked to brassinosteroid biosynthesis. Overall, SA–MJ interactions were trait-dependent, showing clearer synergy in biomass-related performance, while FG primarily contributed to late-stage metabolic adjustments. These findings provide a mechanistic basis for optimizing high-quality cultivation and sustainable utilization of C. pilosula.</p>2026-03-242026-03-24MTBLS14124Chromate (CrO42-), hydrogenCarboxyphosphateChloroacetyl chlorideChloroformCalcium cationCl-Calcium carbimideCalcium formateCryoliteCitraconic acidChlorthiamidCalcium sulfateChloranocrylCypromidChloraminophenamideChlorpyrifos-methylCuminaldehydClopidolCrystal VioletCoumestrol diacetateChlorobiocic acidCloxacillinChlorfenacCarumonam sodiumCI Acid Orange 3Cefpodocime proxetilCycloprothrinChlorquinoxChlorobensideC20916CymarinChloroxanthinConiferinCarboxy-N-[(3,4-dichlorophenyl)methyl]-N-(phosphonomethyl)methanamine N-oxideCefotetan disodiumCopper(2+) bis(6,6,7,7,8,8,8-heptafluoro-2,2-dimethyl-5-oxooct-3-en-3-olate)Compound III(S)Cannabidiolic acidCammaconineCer-AS d65:4Cer-ADS d31:1CHEMBL58084Chloral hydrateCalcium oxideCinnamic acidCinnamalChloropicrinChlorine dioxideCalcium hydroxideClenproperolCuprous oxideChlorotetracyclineClorprenalineCis-8,11,14-Eicosatrienoic acidColterolCopper(II) ionophore IClenpenterolCholesteryl laurateChlorfensulphideCarazololClencyclohexerolCetraxate benzyl esterChenodeoxycholic acidChlordimeformChlormadinone acetateClomipheneCostatoneCamoensineCortisone 21-AcetateChloromebuformCycloartenolCucurbitacin SCodonocarpineClobetasol propionateCoixenolideCaribbean ciguatoxin 1CyclizineCyanotriphenylborateCotylenin FChloramphenicol palmitateCPXCrocetin dialdehydeClomipramineClozapineCompound V(S)CimicifugosideCucurbitacin AColumbamineC-CurarineCarnosolCudaCalycanthidineClidiniumCyclobuxine DChlorendic acidCDCA-d4CalcifediolCer-AP t47:3Chlorophyll ACathasterone