MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/m_MTBLS14131_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/m_MTBLS14131_LC-MS_negative_reverse-phase_metabolite_profiling_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/a_MTBLS14131_LC-MS_positive_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/a_MTBLS14131_LC-MS_negative_reverse-phase_metabolite_profiling.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/s_MTBLS14131.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14131<p>Metabolites were annotated based on accurate mass and spectral matching against public databases, including the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Human Metabolome Database (HMDB).</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed on an ACQUITY UPLC HSS T3 column (100 mm × 2.1 mm, 1.8 μm, Waters). The mobile phases consisted of 0.1% formic acid in water (A) and acetonitrile (B). The flow rate was 0.40 mL/min and the column temperature was 40°C.</p>Amino acid partitioned plant microbiome strategies sustain crop performance under drought.LD ZInstitute of Urban Environment, Chinese Academy of Sciencesrootmass spectrometry assay<p>Freeze-dried samples (100 mg) were extracted with 800 μL of methanol–water (4:1, v/v) containing an internal standard. Samples were homogenized using a cryogenic grinder, followed by ultrasonic extraction at low temperature. After centrifugation and standing, the supernatant was collected for subsequent analysis.</p>Sorghum bicolorhttps://www.ebi.ac.uk/metabolights/MTBLS14131Liandong Zhang. Institute of Urban Environment, Chinese Academy of Sciences. No. 88 Zhongke Road, Chunxiao Town, Beilun District, Ningbo, Zhejiang 315800, China. 1141488604z@gmail.com.<p>Raw data were processed to generate peak intensity matrices, including peak detection, alignment, filtering, and normalization. Missing values were imputed and features with high variability in quality control samples were removed.</p>TreatmentVarietyGrowth stage1141488604z@gmail.com<p>Root exudate samples were collected from sorghum (Sorghum bicolor) grown under control and severe drought conditions across four growth stages in Shanxi, China. Roots were carefully washed to remove adhering soil and then incubated in sterile deionized water for 24 h to collect exudates. Collected samples were subsequently processed and stored under appropriate conditions prior to metabolomic analysis.</p>MetabolomicsThermo Scientific Vanquish UHPLC SystemSorghum bicolordroughtThermo Scientific Orbitrap Exploris 240untargeted analysisplantrootexperimental blankuntargeted metabolite profilingThermo Scientific Vanquish UHPLC SystemSorghum bicolordroughtuntargeted analysisThermo Scientific Orbitrap Exploris 240rootplantexperimental blankuntargeted metabolite profiling<p>Mass spectrometric analysis was performed using an Orbitrap Exploris 240 mass spectrometer equipped with an electrospray ionization (ESI) source operating in both positive and negative modes. The scan range was m/z 70–1050.</p>falseUntargeted LC–MS metabolomics analysis of sorghum root exudates under drought stress<p>This study presents an untargeted metabolomics analysis of root exudates from sorghum (Sorghum bicolor) under control and severe drought conditions. Samples were collected from experimental sites in Shanxi, China. The study aims to investigate metabolic changes in sorghum root exudates in response to drought stress.</p>2026-06-092026-03-24MTBLS14131