Compound identification was based on accurate mass (error <5 ppm), MS/MS fragmentation, retention time matching with standards, and literature comparison.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Gas Chromatography MS - negative - high-polarity"],"chromatography_protocol":["Untargeted metabolomic profiling was conducted on a Vanquish UHPLC system (Thermo Fisher Scientific, USA) coupled to an Orbitrap Exploris 120 high-resolution mass spectrometer equipped with an electrospray ionization (ESI) source. Chromatographic separation was carried out on an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm; Waters, USA) maintained at 35 °C. The mobile phase consisted of 0.1% aqueous formic acid (A) and methanol (B) at a flow rate of 0.4 mL/min. The gradient program was as follows: 0–3 min, 5–10% B; 3–10 min, 10–30% B; 10–20 min, 30–40% B; 20–30 min, 40–100% B; 30–32 min, 100% B; and 33–38 min, 5% B for column re-equilibration. The column temperature was maintained at 35 °C, and the injection volume was 4 µL.
"],"publication":["Synbiotic microcapsules delivering Lactiplantibacillus Plantarum AR113 protect against acute pancreatitis via acetate-mediated modulation of the gut-pancreas axis."],"submitter_name":["Duo Keai"],"submitter_affiliation":["Peking University"],"organism_part":["feces"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["For sample preparation, capsule contents (0.5 g) from each batch were accurately weighed into a 50 mL centrifuge tube, mixed with 20 mL of 70% aqueous methanol, and ultrasonically extracted at 35 °C for 60 min. After centrifugation, the supernatant was collected. All solutions were stored at 4 °C in the dark until analysis.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14147"],"author":["Wei Xiao. University of Shanghai for Science and Technology. xiao18678170427@163.com.","Yue Zeng. Shanghai Jiao Tong University School of Medicine. carrie_1004@sjtu.edu.cn."],"data_transformation_protocol":["raw MS data were processed using Compound Discoverer 3.4 for feature extraction and alignment.
"],"study_factor":["Group"],"submitter_email":["keaiduoduo998@126.com"],"sample_collection_protocol":["Ten batches of HZXL were kindly provided by Taihe Hospital of Traditional Chinese Medicine (Fuyang, China), with batch numbers 21060901, 21071201, 21081601, 21082301, 22072801, 22062901, 21081801, 22093001, 22112501, and 22121701
"],"omics_type":["Metabolomics"],"study_design":["Gut microbiota","Metabolomics","metabolite profiling assay","Mus musculus","Acute pancreatitis","Agilent 5977B MSD","Acetate","Synbiotic microcapsule","feces","Lactiplantibacillus plantarum","Agilent 8890 GC","experimental sample"],"curator_keywords":["Gut microbiota","Metabolomics","Mus musculus","metabolite profiling assay","Acute pancreatitis","Agilent 5977B MSD","Acetate","Synbiotic microcapsule","feces","Agilent 8890 GC","Lactiplantibacillus plantarum","experimental sample"],"mass_spectrometry_protocol":["Mass spectrometric detection was performed in both positive and negative ionization modes over a full scan range of m/z 150–1500. The ESI source parameters were optimized as follows: spray voltage, 3.5 kV in positive mode and 2.5 kV in negative mode; sheath gas pressure, 50 arbitrary units; auxiliary gas pressure, 12 arbitrary units; ion transfer tube temperature, 325 °C; and vaporizer temperature, 350 °C. Data-dependent MS/MS acquisition was performed for structural elucidation using a normalized collision energy of 30%. Instrument control and data acquisition were managed by Xcalibur software (version 4.1, Thermo Fisher Scientific).Untargeted metabolomic profiling was conducted on a Vanquish UHPLC system (Thermo Fisher Scientific, USA) coupled to an Orbitrap Exploris 120 high-resolution mass spectrometer equipped with an electrospray ionization (ESI) source.
"],"metabolite_name":["Isobutyric acid","Valeric acid","Isohexanoic acid","Isovaleric acid","Butanoic acid","Hexanoic acid","Acetic acid","Propanoic acid"],"additional_accession":[]},"is_claimable":false,"name":"Synbiotic microcapsules delivering Lactiplantibacillus Plantarum AR113 protect against acute pancreatitis via acetate-mediated modulation of the gut-pancreas axis","description":"AM-AR113 protects against AP by improving gut microbial composition, enhancing microbial acetate production, promoting intestinal barrier integrity, and suppressing pancreatic M1 macrophage polarization.","dates":{"publication":"2026-03-26","submission":"2026-03-26"},"accession":"MTBLS14147","cross_references":{"HMDB":["HMDB0000042","HMDB0000237","HMDB0000039","HMDB0001873","HMDB0000892","HMDB0000718","HMDB0000535","HMDB0000689"]}}