MetaboLights

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Chromatographic separation was carried out on an ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm; Waters, USA) maintained at 35 °C. The mobile phase consisted of 0.1% aqueous formic acid (A) and methanol (B) at a flow rate of 0.4 mL/min. The gradient program was as follows: 0–3 min, 5–10% B; 3–10 min, 10–30% B; 10–20 min, 30–40% B; 20–30 min, 40–100% B; 30–32 min, 100% B; and 33–38 min, 5% B for column re-equilibration. The column temperature was maintained at 35 °C, and the injection volume was 4 µL.Synbiotic microcapsules delivering Lactiplantibacillus Plantarum AR113 protect against acute pancreatitis via acetate-mediated modulation of the gut-pancreas axis.Duo KeaiPeking Universityfecesmass spectrometry assayFor sample preparation, capsule contents (0.5 g) from each batch were accurately weighed into a 50 mL centrifuge tube, mixed with 20 mL of 70% aqueous methanol, and ultrasonically extracted at 35 °C for 60 min. After centrifugation, the supernatant was collected. All solutions were stored at 4 °C in the dark until analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14147Wei Xiao. University of Shanghai for Science and Technology. xiao18678170427@163.com.Yue Zeng. Shanghai Jiao Tong University School of Medicine. carrie_1004@sjtu.edu.cn.raw MS data were processed using Compound Discoverer 3.4 for feature extraction and alignment.Groupkeaiduoduo998@126.comTen batches of HZXL were kindly provided by Taihe Hospital of Traditional Chinese Medicine (Fuyang, China), with batch numbers 21060901, 21071201, 21081601, 21082301, 22072801, 22062901, 21081801, 22093001, 22112501, and 22121701MetabolomicsGut microbiotaMetabolomicsmetabolite profiling assayMus musculusAcute pancreatitisAgilent 5977B MSDAcetateSynbiotic microcapsulefecesLactiplantibacillus plantarumAgilent 8890 GCexperimental sampleGut microbiotaMetabolomicsMus musculusmetabolite profiling assayAcute pancreatitisAgilent 5977B MSDAcetateSynbiotic microcapsulefecesAgilent 8890 GCLactiplantibacillus plantarumexperimental sampleMass spectrometric detection was performed in both positive and negative ionization modes over a full scan range of m/z 150–1500. The ESI source parameters were optimized as follows: spray voltage, 3.5 kV in positive mode and 2.5 kV in negative mode; sheath gas pressure, 50 arbitrary units; auxiliary gas pressure, 12 arbitrary units; ion transfer tube temperature, 325 °C; and vaporizer temperature, 350 °C. Data-dependent MS/MS acquisition was performed for structural elucidation using a normalized collision energy of 30%. Instrument control and data acquisition were managed by Xcalibur software (version 4.1, Thermo Fisher Scientific).Untargeted metabolomic profiling was conducted on a Vanquish UHPLC system (Thermo Fisher Scientific, USA) coupled to an Orbitrap Exploris 120 high-resolution mass spectrometer equipped with an electrospray ionization (ESI) source.Isobutyric acidValeric acidIsohexanoic acidIsovaleric acidButanoic acidHexanoic acidAcetic acidPropanoic acidfalseSynbiotic microcapsules delivering Lactiplantibacillus Plantarum AR113 protect against acute pancreatitis via acetate-mediated modulation of the gut-pancreas axisAM-AR113 protects against AP by improving gut microbial composition, enhancing microbial acetate production, promoting intestinal barrier integrity, and suppressing pancreatic M1 macrophage polarization.2026-03-262026-03-26MTBLS14147HMDB0000042HMDB0000237HMDB0000039HMDB0001873HMDB0000892HMDB0000718HMDB0000535HMDB0000689