{"database":"MetaboLights","file_versions":[{"headers":{"Content-Type":["application/json"]},"body":{"files":{"Tabular":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/m_MTBLS14172_LC-MS_positive_reverse-phase_v2_maf.tsv","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/m_MTBLS14172_LC-MS_negative_reverse-phase_v2_maf.tsv"],"Txt":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/s_MTBLS14172.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/a_MTBLS14172_LC-MS_negative_reverse-phase.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/a_MTBLS14172_LC-MS_positive_reverse-phase.txt","ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/i_Investigation.txt"]},"type":"primary"},"statusCode":"OK","statusCodeValue":200}],"scores":null,"additional":{"ftp_download_link":["ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172"],"organism_part":["feces"],"metabolite_identification_protocol":["Metabolites were identified by matching accurate mass (<20 ppm) and MS/MS spectra against HMDB, MassBank, and an in-house library."],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Samples (90 mg) were extracted with 1 mL of pre-cooled methanol/acetonitrile/water (2:2:1, v/v/v), sonicated for 1 hour, incubated at -20°C for 1 hour, and centrifuged at 16,000g for 20 min. The supernatant was dried and reconstituted in 100 µL methanol/water (1:1, v/v)."],"organism":["Ovis aries"],"data_transformation_protocol":["Raw data were processed using MS-DIAL for peak alignment and feature extraction. Features with >50% missing values were removed. Total peak area normalization was applied, and data were UV-scaled using R software."],"study_factor":["Weaning status","Weaning protocol"],"metabolights_link":["https://www.ebi.ac.uk/metabolights/MTBLS14172"],"submitter_email":["zhongtao@sicau.edu.cn"],"sample_collection_protocol":["Fecal samples were collected from the rectum using sterile cotton swabs at three time points: three days before weaning, on the day of weaning, and three days after weaning, for lambs weaned at 30, 40, and 50 days of age, respectively."],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"omics_type":["Metabolomics"],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"study_design":["Metabolomics","Ovis aries","Biological specimen","Weaning","untargeted analysis","Shimadzu Nexera UHPLC system","Feces","Thermo Scientific Q Exactive Plus","untargeted metabolite profiling"],"chromatography_protocol":["Chromatography was performed on a Shimadzu Nexera UHPLC system using an ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm). Mobile phase: 0.1% formic acid in water (A) and acetonitrile (B). Gradient: 0-15 min, 0-100% B. Flow rate: 0.3 mL/min, column temperature: 40°C, injection volume: 6 µL."],"publication":["Deciphering early weaning stress cross-talk between gut microbiota with host gene expression and metabolic adaptation insights from lambs."],"curator_keywords":["Metabolomics","Ovis aries","Biological specimen","Weaning","untargeted analysis","Shimadzu Nexera UHPLC system","Feces","Thermo Scientific Q Exactive Plus","untargeted metabolite profiling"],"submitter_affiliation":["Sichuan Agricultural University"],"submitter_name":["Tao Zhong"],"mass_spectrometry_protocol":["Mass spectrometry was performed on a Thermo Scientific Q Exactive Plus with HESI source. Scan range: 70-1050 m/z, resolution: 70,000. Data were acquired in positive and negative ion modes. MS/MS was triggered for the top 10 precursors with NCE of 20, 30, and 40 eV."],"additional_accession":[]},"is_claimable":false,"name":"Metabolome sequencing data of Hu Lambs","description":"<p>Fecal metabolomic data of Hu sheep collected before and after weaning at 30, 40, and 50 days of age under three weaning strategies: abrupt weaning , single intermittent separation, and two-stage intermittent separation .</p>","dates":{"publication":"2026-03-28","submission":"2026-03-28"},"accession":"MTBLS14172","cross_references":{}}