MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/m_MTBLS14172_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/m_MTBLS14172_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/s_MTBLS14172.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/a_MTBLS14172_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/a_MTBLS14172_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14172fecesMetabolites were identified by matching accurate mass (<20 ppm) and MS/MS spectra against HMDB, MassBank, and an in-house library.mass spectrometry assaySamples (90 mg) were extracted with 1 mL of pre-cooled methanol/acetonitrile/water (2:2:1, v/v/v), sonicated for 1 hour, incubated at -20°C for 1 hour, and centrifuged at 16,000g for 20 min. The supernatant was dried and reconstituted in 100 µL methanol/water (1:1, v/v).Ovis ariesRaw data were processed using MS-DIAL for peak alignment and feature extraction. Features with >50% missing values were removed. Total peak area normalization was applied, and data were UV-scaled using R software.Weaning statusWeaning protocolhttps://www.ebi.ac.uk/metabolights/MTBLS14172zhongtao@sicau.edu.cnFecal samples were collected from the rectum using sterile cotton swabs at three time points: three days before weaning, on the day of weaning, and three days after weaning, for lambs weaned at 30, 40, and 50 days of age, respectively.MetaboLightsPublicMetabolomicsLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseMetabolomicsOvis ariesBiological specimenWeaninguntargeted analysisShimadzu Nexera UHPLC systemFecesThermo Scientific Q Exactive Plusuntargeted metabolite profilingChromatography was performed on a Shimadzu Nexera UHPLC system using an ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm). Mobile phase: 0.1% formic acid in water (A) and acetonitrile (B). Gradient: 0-15 min, 0-100% B. Flow rate: 0.3 mL/min, column temperature: 40°C, injection volume: 6 µL.Deciphering early weaning stress cross-talk between gut microbiota with host gene expression and metabolic adaptation insights from lambs.MetabolomicsOvis ariesBiological specimenWeaninguntargeted analysisShimadzu Nexera UHPLC systemFecesThermo Scientific Q Exactive Plusuntargeted metabolite profilingSichuan Agricultural UniversityTao ZhongMass spectrometry was performed on a Thermo Scientific Q Exactive Plus with HESI source. Scan range: 70-1050 m/z, resolution: 70,000. Data were acquired in positive and negative ion modes. MS/MS was triggered for the top 10 precursors with NCE of 20, 30, and 40 eV.falseMetabolome sequencing data of Hu Lambs<p>Fecal metabolomic data of Hu sheep collected before and after weaning at 30, 40, and 50 days of age under three weaning strategies: abrupt weaning , single intermittent separation, and two-stage intermittent separation .</p>2026-03-282026-03-28MTBLS14172