MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/m_MTBLS14174_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/m_MTBLS14174_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/a_MTBLS14174_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/s_MTBLS14174.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/a_MTBLS14174_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174/i_Investigation.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14174Metabolites were identified by matching accurate mass (<20 ppm) and MS/MS spectra against HMDB, MassBank, and an in-house library.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phaseChromatography was performed on a Shimadzu Nexera UHPLC system using an ACQUITY UPLC HSS T3 column (2.1 × 100 mm, 1.8 µm). Mobile phase: 0.1% formic acid in water (A) and acetonitrile (B). Gradient: 0-15 min, 0-100% B. Flow rate: 0.3 mL/min, column temperature: 40°C, injection volume: 6 µL.Deciphering early weaning stress cross-talk between gut microbiota with host gene expression and metabolic adaptation insights from lambs.Tao ZhongSichuan Agricultural Universityserummass spectrometry assayFor metabolite extraction, 100 μL of serum sample was mixed with 300 μL of pre-chilled methanol/acetonitrile/water (2:2:1, v/v/v). The mixture was vortexed for 30 seconds, sonicated in an ice bath for 1 hour, then incubated at -20°C for 1 hour to precipitate proteins. After centrifugation at 16,000 × g for 20 minutes at 4°C, the supernatant was collected and dried in a vacuum concentrator. For LC-MS analysis, the dried extract was reconstituted in 100 μL of methanol/water (1:1, v/v), centrifuged at 20,000 × g for 20 minutes at 4°C, and the supernatant was used for analysis. A pooled quality control (QC) sample was prepared by mixing equal volumes of all samples and was used to monitor system stability throughout the analysis.Ovis ariesRaw data were processed using MS-DIAL for peak alignment and feature extraction. Features with >50% missing values were removed. Total peak area normalization was applied, and data were UV-scaled using R software.Weaning statusWeaning protocolzhongtao@sicau.edu.cnhttps://www.ebi.ac.uk/metabolights/MTBLS14174Serum samples were collected from Hu sheep at 30 days of age before and after weaning. Blood samples were collected from the jugular vein into vacuum tubes without anticoagulant, allowed to clot at room temperature for 30 minutes, and then centrifuged at 3,000 × g for 10 minutes. The serum was separated and immediately frozen in liquid nitrogen, then stored at -80°C until analysis.MetabolomicsMetabolomicsOvis ariesBiological specimenWeaninguntargeted analysisSerumShimadzu Nexera UHPLC systemThermo Scientific Q Exactive Plusuntargeted metabolite profilingMetabolomicsOvis ariesBiological specimenWeaninguntargeted analysisSerumShimadzu Nexera UHPLC systemThermo Scientific Q Exactive Plusuntargeted metabolite profilingMass spectrometry was performed on a Thermo Scientific Q Exactive Plus with HESI source. Scan range: 70-1050 m/z, resolution: 70,000. Data were acquired in positive and negative ion modes. MS/MS was triggered for the top 10 precursors with NCE of 20, 30, and 40 eV.TaurineAminohippuric acidAscorbic acidD-LeucinePhenylacetylglutamine2H-Pyrido[1,2-a][1,4,7,10]tetraazacyclododecine-1,4,7,10(3H,12H)-tetrone, 3-(7,8-dihydroxy-6-oxooctyl)octahydro-9-(1-methylpropyl)-6-(phenylmethyl)-Cholic acidPC(16:0/18:2)6-Benzyl-3-butan-2-yl-9-(7-hydroxy-6-oxooctyl)-1,4,7,10-tetrazabicyclo[10.4.0]hexadecane-2,5,8,11-tetroneHistidineSuccinoadenosinePC(16:0/16:0)N-CaproylglycineTropic acidLPC(15:0)Kajiichigoside F13-MethylhistidinePhosphoric acidThreonine6-Hydroxy-2,6,10,10-tetramethyl-1-oxaspiro[4.5]dec-8-yl 6-O-[(2R,3R,4R)-3,4-dihydroxy-4-(hydroxymethyl)tetrahydro-2-furanyl]-beta-D-glucopyranoside(2E,6E,12E)-19-(2-Amino-2-oxoethyl)-9,11-dihydroxy-8-methoxy-10,12,14-trimethyl-15-oxohenicosa-2,6,12-trienedioic acid[9-[(E)-3-acetyloxy-4-methylhex-4-en-2-yl]-12-hydroxy-3-(2-hydroxypropan-2-yl)-10-methoxy-6a,12b-dimethyl-8,11-dioxo-1,2,3,4a,5,6,12,12a-octahydropyrano[3,2-a]xanthen-1-yl] acetate4-Hydroxyhippuric acid16,17-Dihydroxykauran-18-oic acid4-Hydroxybenzoic acidBonactinAvocadyne 1-acetateCitrullineCorylifol APropionylcarnitine1,10-EpoxygermacroneSebacic acidPhenolSalicinCarnequinazoline AAcetaminophenSphinganine 1-phosphate3',4'-Dimethoxyflavonol6,8-Dihydroxy-2-methylmercaptopurinecyclo[DL-Nle(Unk)-D-Phe-xiIle-Pip]LPG(17:0)Ergothioneine2-[5,7-dihydroxy-3-oxo-6-[(2E,6E)-3,7,11-trimethyldodeca-2,6,10-trienyl]-1H-isoindol-2-yl]pentanedioic acidGlutamic acidSuberic acid[1-(7-Hydroxy-2,4b,8,8-Tetramethyl-4,4a,5,6,7,8a,9,10-Octahydro-3H-Phenanthren-2-Yl)-2-[3,4,5-Trihydroxy-6-(Hydroxymethyl)Oxan-2-Yl]Oxyethyl] AcetateLPE(18:0)LincomycinArginineKynurenineTaurohyocholateChenodeoxycholylaspartic acidLPC(17:0)N-Methylglutamic acidGlucosamine 6-phosphateCreatinineN-Acetylglutamic acidTaurolithocholic acidPhenylalanineDodecanedioic acidTryptophanPhenylacetylglycine5-Hydroxylysine(2E)-3-{3-[(2-cyclohexylphenoxy)methyl]-4-methoxyphenyl}-1-(4-hydroxyphenyl)pr op-2-en-1-onePyridoxineN-AcetylisoleucinePseudouridinePimelic acidXanthineProlylhydroxyprolineCitric acidLPE(18:1)EmodinPoricoic acid A3h-Indole-3-propanoic acid,a-amino-[6,10a-Dihydroxy-4-(hydroxymethyl)-4,7,11b-trimethyl-9-oxo-1,2,3,4a,5,6,6a,7,11,11a-decahydronaphtho[2,1-][1]benzouran-5-yl] acetateGlycolithocholic acidPC(16:0/18:1)5-[5-Hydroxy-3-(hydroxymethyl)pentyl]-8a-(hydroxymethyl)-5,6-dimethyl-3,4,4a,6,7,8-hexahydronaphthalene-1-carboxylic acidAsparagine2-(3-phenylpropanamido)acetic acidLPC(14:0)[(2-indol-3-ylethyl)amino]-N-(2-methoxyphenyl)carboxamideUracilN-Acetylneuraminic acidPantothenic acidCapryloyl glycine(2E)-3-(4-Acetoxy-2,3-dihydroxy-2,5,5,8a-tetramethyldecahydro-1-naphthalenyl)acrylic acidGuanosineCortisolThymidineCreatine3-phenyl-2-pyrrolylpropanoic acid(23R,24R)-3beta-(Beta-D-Xylopyranosyloxy)-9beta,19-Cyclo-16beta,23:16,24-Diepoxy-5alpha-Lanosta-7-Ene-15alpha,25-DiolEfavirenzShikimic acid4-Methyoxybenzoyl-N-glycineCytochalasin BGlycocholatePyrrolo[1,2-d][1,4,7,10,13,16]oxapentaazacyclononadecine-1,4,7,10,14,17(11H,16H)-hexone, 16-(3-chloro-2-hydroxypropyl)dodecahydro-5,8,9-trimethyl-6-(1-methylethyl)-3-(1-methylpropyl)-LPA(18:1)AcetylglycineLPC(16:1)Methionine sulfoneChartarlactam I8-[2-(3,4-dihydroxy-2,5-dimethoxyoxolan-3-yl)ethyl]-4a,7,8-trimethylspiro[2,3,5,6,7,8a-hexahydro-1H-naphthalene-4,2'-oxirane]-2,3-diolMetacetamolGlutamylleucineTyrosinePruninConglobatinGallic acid3-Phenyllactic acidCinnamoylglycine2,3,4-Trihydroxy-4'-ethoxybenzophenoneTetradecanoylphorbol acetateVitamin K27,8-Dihydroxyflavonecis-Aconitate(4-oxido-2,3,5,6,7,8-hexahydro-1H-pyrrolizin-4-ium-1-yl)methyl 2,3-dihydroxy-3-methylpentanoateHypoxanthineChenodeoxycholic acid1-CarboxyethylphenylalanineOrnithineLPC(18:2)(E)-4-[(1R,2S,3S,4R,8aS)-2,3,4-trihydroxy-2,5,5,8a-tetramethyl-3,4,4a,6,7,8-hexahydro-1H-naphthalen-1-yl]but-3-en-2-oneHippuric acid5-HydroxymethylcytosineEnterolactoneFusidic acidTaurodeoxycholic acid[1-(3,5-Dihydroxyphenyl)-12-hydroxytridecan-2-yl] acetateN-(4-methylphenyl)-2-(1-methyl-3-phenylthioindol-2-yl)acetamideGlutaminylarginineIndole-3-Lactic AcidLPC(18:1)2-[(2,5-dimethyl(1,3-oxazolino[4,5-e]pyrimidin-7-yl))amino]-4-methylpentanoic acid5-O-MethyllicoricidinIndole-3-acetic acid7-Benzofurancarboxylic acid, 2,3-dihydro-2-(1-hydroxy-1-methylethyl)-4-methoxy-6-(2-phenylethyl)-3Beta-Hydroxy-23,24-Bisnorchol-5-Enic AcidSuberylglycine5-HydroxyindoleacetateUric acidPhosphocholineDeoxycholic acid3-Hydroxyflavone15,16-DiHODEDeoxycytidineTMPCholylalanineGlycodeoxycholic acidPC(18:0/20:4)8,11,14-Anacardic Acid3,15-dibenzyl-1,4,12-trimethyl-1,4,7,10,13-pentazacyclopentadecane-2,5,8,11,14-pentoneVanillinNcgc00385436-01!GlutaminefalseMetabolome sequencing data of Hu Lambs<p>Serum metabolomic data of Hu sheep collected before and after weaning at 30, 40, and 50 days of age under three weaning strategies: abrupt weaning, single intermittent separation, and two-stage intermittent separation.</p>2026-03-282026-03-28MTBLS14174MTBLC64575MTBLC88811MTBLC88465CHEBI:64575CHEBI:88811CHEBI:88465