The raw data were converted to the mzXML format using ProteoWizard and processed with an in-house program. which was developed using R and based on XCMS, for peak detection, extraction, alignment, and integration. The R package and the BiotreeDB(V3.0) were applied in metabolite identification.
"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The animal tissue samples (25 mg±1 mg) were taken, mixed with beads and 500 μL of extraction solution (MeOH:ACN:H2O, 2:2:1 (v/v)). The extraction solution contain deuterated internal standards. The mixed solution were vortexed for 30 s.
"],"organism":["Cyprinus carpio"],"data_transformation_protocol":["The raw data were converted to the mzXML format using ProteoWizard and processed with an in-house program. which was developed using R and based on XCMS, for peak detection, extraction, alignment, and integration. The R package and the BiotreeDB(V3.0) were applied in metabolite identification.
"],"study_factor":["Treatment"],"metabolights_link":["https://www.ebi.ac.uk/metabolights/MTBLS14203"],"submitter_email":["18706599910@163.com"],"sample_collection_protocol":["They were left in the anaesthetic bath and monitored for 20 min to ensure death via an overdose of MS222 and to ensure they did not regain consciousness, For each cage, thirteen fish were randomly collected for sampling.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"omics_type":["Metabolomics"],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"study_design":["Emodin","Thermo Scientific Vanquish UHPLC System","Immunostimulant","Metabolomics","carp","untargeted analysis","Thermo Scientific Orbitrap Exploris 120","Aeromonas hydrophila","Cyprinus carpio","hindgut","Aquaculture","untargeted metabolite profiling"],"chromatography_protocol":["For polar metabolites, LC-MS/MS analyses were performed using an UHPLC system (Vanquish, Thermo Fisher Scientific) with a Waters ACQUITY UPLC BEH Amide (2.1 mm × 50 mm, 1.7 μm) coupled to Orbitrap Exploris 120 mass spectrometer (Orbitrap MS, Thermo). The mobile phase consisted of 25 mmol/L ammonium acetate and 25 ammonia hydroxide in water(pH = 9.75)(A) and acetonitrile (B). The auto-sampler temperature was 4 °C, and the injection volume was 2 μL.
"],"publication":["Multi-Omics integration reveals that Emodin enhances resistance to Aeromonas hydrophila in Yellow River Carp (Cyprinus carpio haematopterus) by modulating the gut microbiota-metabolism-immune Axis."],"curator_keywords":["Thermo Scientific Vanquish UHPLC System","Emodin","Immunostimulant","Metabolomics","carp","untargeted analysis","Thermo Scientific Orbitrap Exploris 120","Aeromonas hydrophila","Cyprinus carpio","hindgut","Aquaculture","untargeted metabolite profiling"],"submitter_affiliation":["Henan Agricultural University"],"submitter_name":["Liu YuQing"],"mass_spectrometry_protocol":["The Orbitrap Exploris 120 mass spectrometer was used for its ability to acquire MS/MS spectra on information-dependent acquisition (IDA) mode in the control of the acquisition software (Xcalibur, Thermo). In this mode, the acquisition software continuously evaluates the full scan MS spectrum. The ESI source conditions were set as following: sheath gas flow rate as 50 Arb, Aux gas flow rate as 15 Arb, capillary temperature 320 °C, full MS resolution as 60000, MS/MS resolution as 15000, collision energy: SNCE 20/30/40, spray voltage as 3.8 kV (positive) or -3.4 kV (negative), respectively.
"],"additional_accession":[]},"is_claimable":false,"name":"Multi-Omics integration reveals that Emodin enhances resistance to Aeromonas hydrophila in Yellow River Carp (Cyprinus carpio haematopterus) by modulating the gut microbiota-metabolism-immune Axis","description":"Multi-Omics integration reveals that Emodin enhances resistance to Aeromonas hydrophila in Yellow River Carp (Cyprinus carpio haematopterus) by modulating the gut microbiota-metabolism-immune Axis","dates":{"publication":"2026-04-01","submission":"2026-04-01"},"accession":"MTBLS14203","cross_references":{}}