The processed data were analyzed by R package (ropls), where it was subjected to multivariate data analysis, including Pareto-scaled principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). The 7-fold cross-validation and response permutation testing were used to evaluate the robustness of the model. The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Student's t test was applied to determine the significance of differences between two groups of independent samples. p value < 0.05 were used to screen significant changed metabolites. Pearson's correlation analysis was performed to determine the correlation between two variables.Commercial databases including was used for pathway enrichment analysis.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - alternating - hilic","Liquid Chromatography MS - alternating - reverse-phase"],"chromatography_protocol":["Analyses were performed using an UHPLC (1290 Infinity LC, Agilent Technologies) coupled to a QTRAP MS (6500+, AB Sciex) in Shanghai Applied Protein Technology Co., Ltd. The analytes were separated on HILIC (Waters UPLC BEH Amide column, 2.1 mm 100 mm, 1.7 um) and C18 columns (Waters UPLC BEH C18-2.1x100 mm, 1.7 um).
For HILIC separation, the column temperature was set at 35 degrees Celsius and the injection volume was 2 uL. Mobile phase A: 90% H2O + 2 mM ammonium formate + 10% acetonitrile , mobile phase B: 0.4% formic acid in acetonitrile. A gradient (85% B at 0-1 min, 80% B at 3-4 min, 70% B at 6 min, 50% B at 10-15.5 min, 85% B at 15.6 -23 min ) was then initiated at a flow rate of 300 μL/min.
For RPLC separation, the column temperature was set at 40 degrees Celsius and the injection volume was 2 uL. Mobile phase A: 5 mM ammonium acetate in water, mobile phase B: 99.5% acetonitrile.A gradient (5% B at 0 min, 60% B at 5 min, 100% B at 11-13 min, 5% B at 13.1-16 min ) was then initiated at a flow rate of 400 uL/min. The sample was placed at 4 degrees Celsius during the whole analysis process.
"],"publication":["ACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload by regulating histone isobutyrylation."],"submitter_name":["Xinyan Zhao"],"submitter_affiliation":["Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences"],"organism_part":["Heart"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["The animal tissues (e.g., mice liver) were quickly frozen in liquid nitrogen immediately after dissection. Then the tissue was cut on dry ice (~80 mg) into an Eppendorf tube (2 mL). To extract metabolites from tissue samples, 1000 uL of cold Methanol / acetonitrile / water (2:2:1, v/v) extraction solvent was added to 80 mg sample, and adequately vortexed. For absolute quantification of the metabolites, stock solutions of stable-isotope internal standards were added to the extraction solvent simultaneously. The samples was homogenized by MP homogenizer (24 x 2, 6.0 M/S, 60 s, twice) and sonicated at 4 degrees Celsius (30min/once, twice) then centrifuged at 14,000 g for 20 minutes at 4degrees Celsius and the supernatant was dried in a vacuum centrifuge at 4°C. For LC-MS analysis, the samples were re-dissolved in 100 uL acetonitrile/water (1:1, v/v) solvent and centrifuged at 14000 g at 4degrees Celsius for 15 min, then the supernatant was injected.
"],"organism":["Mus musculus"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14207"],"author":["Xinyan Zhao. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. lily2000222@126.com.","Xin Sun.","Kexin Si.","Heping Wang.","Xiang Wei.","Yuefei Zhang.","Depei Liu. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences. liudp@pumc.edu.cn.","Xiaoqiang Tang. West China Second University Hospital, Sichuan University. tangxiaoqiang@scu.edu.cn.","Houzao Chen. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences. chenhouzao@ibms.cams.cn.","Jingyi Wang. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. jywangburn@163.com.","Dingsheng Jiang.","Huiyu Wang."],"data_transformation_protocol":["MultiQuant or Analyst was used for quantitative data processing. The QCs were processed together with the biological samples. Metabolites in QCs with coefficient of variation (CV) less than 30 % were denoted as reproducible measurements.
"],"study_factor":["Genotype"],"submitter_email":["lily2000222@126.com"],"sample_collection_protocol":["Acad8flox/flox mice were obtained from the Experimental Animal Tech Co. of Saiye (Beijing, China). The Myh6-Cre (αMHC-MerCreMer) transgenic mice were obtained from Jackson Laboratory. Male C57BL/6 mice (8 weeks old) were obtained from Vital River Laboratory Animal Technology Co., Ltd. Acad8cKO mice were generated by crossing Acad8flox/flox mice with Myh6-Cre (αMHC-MerCreMer) transgenic mice. Conditional cardiomyocyte-specific Acad8 knockout was induced by tamoxifen treatment. Mice (6-8 weeks old, body weight > 22 g) were intraperitoneally administered either vehicle (oil) or tamoxifen (40 mg/kg) every other day for a total of two doses to induce Cre activity. Then, we harvested hearts from both Myh6-Cre and Acad8cKO mice that were subjected to TAC surgery.
"],"omics_type":["Metabolomics"],"study_design":["Heart","Metabolomics","AB SCIEX QTRAP 6500+","Mus musculus","targeted analysis","Mouse","cardiac hypertrophy","Agilent 1290 Infinity LC","Timepoint","pooled sample","Chinese Academy of Medical Sciences & Peking Union Medical College"],"curator_keywords":["Heart","Metabolomics","AB SCIEX QTRAP 6500+","Mus musculus","targeted analysis","Mouse","cardiac hypertrophy","Timepoint","Agilent 1290 Infinity LC","pooled sample","Chinese Academy of Medical Sciences & Peking Union Medical College"],"mass_spectrometry_protocol":["6500+ QTRAP (AB SCIEX) was performed in positive and negative switch mode. The ESI positive source conditions were as follows: Source temperature: 580 degrees Celsius Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain Gas (CUR): 35; IonSpray Voltage (IS): +4500 V; The ESI negative source conditions were as follows: Source temperature: 580 degrees Celsius Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain gas (CUR): 35; IonSpray Voltage(IS): -4500 V. MRM method was used for mass spectrometry quantitative data acquisition. The MRM ion pairs are showed in the attached file. A polled quality control (QC) samples were set in the sample queue to evaluate the stability and repeatability of the system.
"],"metabolite_name":["Taurodeoxycholic acid (TDCA)","Docosanoic Acid","Corticosterone","Myristelaidic acid","Palmitic acid","Arachidonic acid","Protocatechuic acid","Kynurenate","N-Acetylmethionine","2-Pyrocatechuic acid","Cortexolone","Quinoline","Indole-3-methyl acetate","5-Valerolactone","Elaidic Acid","11Z,14Z,17Z-Eicosatrienoic Acid","Caffeine","Myristoleic acid","m-Aminobenzoic acid","Benzenebutanoic acid","Palmitoylethanolamide","Indole-3-lactic Acid","Flavone","4-Hydroxyhippuric acid","4-Hydroxybenzoic acid","Beta-Hyodeoxycholic acid (¦Â-HDCA)","Gamma-Linolenic acid","Isolithocholic acid (IsoLCA)","Hyocholic acid (HCA)","Sebacic acid","Cinnavalininate","7-Ketolithocholic acid (7-KLCA)","Phenol","Tauro-¦Á-muricholic acid (¦Á-TMCA)","7-Dehydrocholic acid (7-DHCA)","Retinoic acid","Theophylline","4-Hydroxybenzaldehyde","Hexanoylcarnitine","Undecanoic acid","6-Methylthiopurine","Ricinoleic acid","Suberic acid","Isodeoxycholic acid (IsoDCA)","Methylglutaric acid","Pyrrole-2-carboxylic acid","10Z-Heptadecenoic acid","Butyrylcarnitine","Deoxycholic acid (DCA)","Palmitelaidic acid","5'-Methylthioadenosine","3-Methoxytyramine","Quinaldic acid","Oleic acid","10E-Heptadecenoic acid","Murideoxycholic acid","Indoleacetic acid","Taurolithocholic acid (TLCA)","Erucic Acid","3-Methyladipic acid","10Z-Pentadecenoic Acid","Pentadecanoic acid","3-Dehydrocholic acid (3-DHCA)","gamma-Glutamyl-phenylalanine","5,6-Dimethylbenzimidazole","N-Acetylphenylalanine","Hydroxyphenyllactic acid","Ursodeoxycholic acid (UDCA)","N-Methylnicotinamide","Dopamine","Serotonin","Chenodeoxycholic acid £¨CDCA£©","Petroselinic acid","Phenylacetylglycine","Linoelaidic acid","p-Cresyl sulfate","Tauro-¦Â-muricholic acid (¦Â-TMCA)","Decanoylcarnitine","N-Formyl-methionine","Linoleyl carnitine","N-Acetylleucine","Lipoamide","Salicylamide","13Z,16Z-Docosadienoic Acid","Leucylphenylalanine","Valerylcarnitine","Tryptamine","Glycocholic acid £¨GCA£©","10Z-Nonadecaenoic acid","12-hydroxystearic acid","Conjugated linoleic acids (CLA)","Melatonine","Indole-3-acetamide","Glycoursodeoxycholic acid (GUDCA)","Glycolithocholic acid-3-Sulfate","Octanoylcarnitine","Phenylalanylalanine","Vanillic acid","Tryptophanol","Cholesterol sulfate","Indole-3-carboxaldehyde","Indoleacrylic acid","Stearic acid","Tetradecanoylcarnitine","Leucylalanine","3-Phenylbutyric acid","Phthalic acid","Gallic acid","Oleylcarnitine","2-Hydroxycaproic acid","4Z,7Z,10Z,13Z,16Z-Docosapentaenoic Acid","Indoxyl sulfate","2-Hydroxy-3-methylbutyric acid","Cholic acid (CA)","3-Methylindole","Beta-Ursodeoxycholic acid (¦Â-UDCA)","Heneicosanoic acid","N-Acetyltyrosine","Myristic acid","2-Hydroxy-2-methylbutyric acid","Ursocholic acid (UCA)","Phenyllactic acid","Xanthurenate","Adipic acid","Taurocholic acid (TCA)","5'-Deoxyadenosine","Vitamin B7","3-Hydroxybenzoic acid","Hippuric acid","Isovalerylcarnitine","3-Aminosalicylic acid","7Z,10Z,13Z,16Z-Docosatetraenoic acid","Phenylethylamine","Caffeate","Dodecanoic acid","Benzamide","Alpha-Linolenic Acid","4-Hydroxyphenylpyruvic acid","Dihomo-gamma-linolenic acid","Azelaic acid","Beta-Muricholic acid (¦Â-MCA)","Tridecanoic acid","4Z,7Z,10Z,13Z,16Z,19Z-Docosahexaenoic Acid (DHA)","3-Methyloxindole","Hyodeoxycholic acid £¨HDCA£©","Taurochenodeoxycholic acid (TCDCA)","11Z-Eicosenoic Acid","Epinephrine","N-Methyltryptamine","Lithocholic acid £¨LCA£©","Palmitoleic Acid","Dethiobiotin","2',4'-Dihydroxyacetophenone","Chlorogenic acid","5Z,8Z,11Z,14Z,17Z-Eicosapentaenoic Acid","2-Hydroxycinnamic acid","2-Hydroxyphenethylamine","Linoleic acid","Indole-3-carboxylic acid","Nordeoxycholic acid (NorDCA)","Heptadecanoic acid","Citraconic acid","1-Oleylglycerol","Vanillin","Stearylcarnitine","Lumichrome","Glycyl-Phenylalanine","Glutamyltryptophan"],"additional_accession":[]},"is_claimable":false,"name":"ACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload by regulating histone isobutyrylation","description":"Branched-chain amino acids play critical roles in cardiac physiology and diseases. Genetic deficiency in the valine catabolic enzyme ACAD8 is clinically associated with isobutyryl-CoA deregulation and cardiomyopathy in humans. To explore the mechanism underlying cardiac pathological hypertrophy in Acad8cKO mice, we performed targeted metabolomic analysis of hearts from both Myh6-Cre and Acad8cKO mice that were subjected to TAC surgery.","dates":{"publication":"2026-04-15","submission":"2026-04-02"},"accession":"MTBLS14207","cross_references":{"MetaboLights":["MTBLC145573"],"HMDB":["HMDB0000824","HMDB0005768","HMDB0029098","HMDB0028853","HMDB0002095","HMDB0028685","HMDB0011733","HMDB0028854","HMDB0001406","HMDB0000134","HMDB0000017","HMDB0000965","HMDB0034155","HMDB0304940","HMDB0013318","HMDB0000289","HMDB0004326","HMDB0013751","HMDB0000318","HMDB0000300","HMDB0000239","HMDB0000130","HMDB0004366","HMDB0003331","HMDB0001366","HMDB0000101","HMDB0004044","HMDB0000034","HMDB0257047","HMDB0000157","HMDB0000050","HMDB0000296","HMDB0003466","HMDB0000064","HMDB0000562","HMDB0001392","HMDB0000754","HMDB0000244","HMDB0000802","HMDB0003192","HMDB0000895","HMDB0000462","HMDB0000008","HMDB0029965","HMDB0001488","HMDB0000195","HMDB0000132","HMDB0000226","HMDB0000292","HMDB0001867","HMDB0000089","HMDB0001431","HMDB0304941","HMDB0000159","HMDB0000929","HMDB0001129","HMDB0011616","HMDB0000011","HMDB0060080","HMDB0002658","HMDB0001434","HMDB0003911","HMDB0000684","HMDB0003349","HMDB0000301","HMDB0000133","HMDB0000299","HMDB0000687","HMDB0001645","HMDB0002024","HMDB0002210","HMDB0000766","HMDB0001200","HMDB0000026","HMDB0000557","HMDB0000172","HMDB0000759","HMDB0001522","HMDB0000210","HMDB0000696","HMDB0000043","HMDB0004437","HMDB0000058","HMDB0000700","HMDB0000532","HMDB0000875","HMDB0000097","HMDB0000247","HMDB0000943","HMDB0002064","HMDB0000238","HMDB0013716","HMDB0000765","HMDB0000087","HMDB0002271","HMDB0000201","HMDB0006029","HMDB0001861","HMDB0000472","HMDB0004824","HMDB0003431","HMDB0000883","HMDB0000158","HMDB0000092","HMDB0000107","HMDB0000251","HMDB0000267","HMDB0000235","HMDB0000716","HMDB0094692","HMDB0000162","HMDB0001514","HMDB0000870","HMDB0000925","HMDB0001906","HMDB0245095","HMDB0036458","HMDB0000613","HMDB0000991","HMDB0006028","HMDB0000622","HMDB0000271","HMDB0000001","HMDB0000725","HMDB0000056","HMDB0000161","HMDB0003464","HMDB0000867","HMDB0003072","HMDB0000062","HMDB0000167","HMDB0000128","HMDB0060460","HMDB0001149","HMDB0000719","HMDB0029430","HMDB0001161","HMDB0000123","HMDB0000479","HMDB0003070","HMDB0000641","HMDB0000355","HMDB0003355","HMDB0000177","HMDB0000208","HMDB0000112","HMDB0000187","HMDB0000168","HMDB0001982","HMDB0000446","HMDB0000055","HMDB0000211","HMDB0000679","HMDB0000939","HMDB0001844","HMDB0000661","HMDB0002393","HMDB0000625","HMDB0002092","HMDB0062660","HMDB0002757","HMDB0001901","HMDB0000740","HMDB0000186","HMDB0000904","HMDB0029415","HMDB0000426","HMDB0000975","HMDB0000691","HMDB0002006","HMDB0000254","HMDB0000606","HMDB0000812","HMDB0001138","HMDB0005765","HMDB0000148","HMDB0000194","HMDB0001248","HMDB0000156","HMDB0000191","HMDB0002329","HMDB0006248","HMDB0000742","HMDB0002284","HMDB0000033","HMDB0000510","HMDB0001539","HMDB0001227","HMDB0000905","HMDB0002520","HMDB0000192","HMDB0001185","HMDB0001409","HMDB0000663","HMDB0001178","HMDB0000045","HMDB0000121","HMDB0000126","HMDB0003269","HMDB0001517","HMDB0006557","HMDB0001413","HMDB0000304","HMDB0000290","HMDB0000072","HMDB0000288","HMDB0001370","HMDB0001548","HMDB0000175","HMDB0001341","HMDB0000286","HMDB0000224","HMDB0000229","HMDB0000193","HMDB0011747","HMDB0000302","HMDB0000958","HMDB0001325","HMDB0001078","HMDB0000124","HMDB0000379","HMDB0001586","HMDB0000094","HMDB0000095","HMDB0001397","HMDB0000263","HMDB0000362","HMDB0001254","HMDB0000272","HMDB0004110","HMDB0001532","HMDB0000295","HMDB0001401","HMDB0001107","HMDB0000214","HMDB0000645","HMDB0002666","HMDB0000960","HMDB0001163","HMDB0000517","HMDB0001554","HMDB0000935","HMDB0000450","HMDB0000182","HMDB0000189","HMDB0006024","HMDB0000206","HMDB0000002","HMDB0000258","HMDB0002005","HMDB0001209","HMDB0000086","HMDB0034367","HMDB0011172","HMDB0001243","HMDB0001067","HMDB0001109","HMDB0001095","HMDB0000998","HMDB0000225","HMDB0000202","HMDB0000306","HMDB0001866","HMDB0000291","HMDB0005066","HMDB0005065","HMDB0006469","HMDB0000848","HMDB0028830","HMDB0028922","HMDB0028988","HMDB0029562","HMDB0028848","HMDB0013243","HMDB0002013","HMDB0013128","HMDB0000688","HMDB0000756","HMDB0000791","HMDB0000651","HMDB0000653","HMDB0003075","HMDB0012308","HMDB0000068","HMDB0001972","HMDB0000073","HMDB0005807","HMDB0000634","HMDB0000259","HMDB0003164","HMDB0001987","HMDB0000036","HMDB0001891","HMDB0013678","HMDB0000022","HMDB0001856","HMDB0001065","HMDB0003152","HMDB0001015","HMDB0000407","HMDB0000682","HMDB0001983","HMDB0000881","HMDB0000755","HMDB0004230","HMDB0000752","HMDB0000866","HMDB0000448","HMDB0004461","HMDB0012275","HMDB0011745","HMDB0000397","HMDB0002466","HMDB0000500","HMDB0000228","HMDB0002107","HMDB0001889","HMDB0003320","HMDB0000714","HMDB0033731","HMDB0000707","HMDB0000821","HMDB0000303","HMDB0000842","HMDB0000484","HMDB0004370","HMDB0001173","HMDB0001964","HMDB0001847","HMDB0011718","HMDB0000555","HMDB0029739","HMDB0000030","HMDB0000826","HMDB0000671","HMDB0060412","HMDB0015687","HMDB0004078","HMDB0000779","HMDB0001624","HMDB0003701","HMDB0011756","HMDB0000512","HMDB0029737","HMDB0000715","HMDB0000893"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