MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/m_MTBLS14207_LC-MS_alternating_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/m_MTBLS14207_LC-MS_alternating_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/a_MTBLS14207_LC-MS_alternating_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/a_MTBLS14207_LC-MS_alternating_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207/s_MTBLS14207.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14207<p>The processed data were analyzed by R package (ropls), where it was subjected to multivariate data analysis, including Pareto-scaled principal component analysis (PCA) and orthogonal partial least-squares discriminant analysis (OPLS-DA). The 7-fold cross-validation and response permutation testing were used to evaluate the robustness of the model. The variable importance in the projection (VIP) value of each variable in the OPLS-DA model was calculated to indicate its contribution to the classification. Student's t test was applied to determine the significance of differences between two groups of independent samples. p value &lt; 0.05 were used to screen significant changed metabolites. Pearson's correlation analysis was performed to determine the correlation between two variables.Commercial databases including was used for pathway enrichment analysis.</p>MetaboLightsPublicLiquid Chromatography MS - alternating - hilicLiquid Chromatography MS - alternating - reverse-phase<p>Analyses were performed using an UHPLC (1290 Infinity LC, Agilent Technologies) coupled to a QTRAP MS (6500+, AB Sciex) in Shanghai Applied Protein Technology Co., Ltd. The analytes were separated on HILIC (Waters UPLC BEH Amide column, 2.1 mm 100 mm, 1.7 um) and C18 columns (Waters UPLC BEH C18-2.1x100 mm, 1.7 um).</p><p>For HILIC separation, the column temperature was set at 35 degrees Celsius and the injection volume was 2 uL. Mobile phase A: 90% H2O + 2 mM ammonium formate + 10% acetonitrile , mobile phase B: 0.4% formic acid in acetonitrile. A gradient (85% B at 0-1 min, 80% B at 3-4 min, 70% B at 6 min, 50% B at 10-15.5 min, 85% B at 15.6 -23 min ) was then initiated at a flow rate of 300 μL/min.</p><p>For RPLC separation, the column temperature was set at 40 degrees Celsius and the injection volume was 2 uL. Mobile phase A: 5 mM ammonium acetate in water, mobile phase B: 99.5% acetonitrile.A gradient (5% B at 0 min, 60% B at 5 min, 100% B at 11-13 min, 5% B at 13.1-16 min ) was then initiated at a flow rate of 400 uL/min. The sample was placed at 4 degrees Celsius during the whole analysis process.</p>ACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload by regulating histone isobutyrylation.Xinyan ZhaoInstitute of Basic Medical Sciences, Chinese Academy of Medical SciencesHeartmass spectrometry assay<p>The animal tissues (e.g., mice liver) were quickly frozen in liquid nitrogen immediately after dissection. Then the tissue was cut on dry ice (~80 mg) into an Eppendorf tube (2 mL). To extract metabolites from tissue samples, 1000 uL of cold Methanol / acetonitrile / water (2:2:1, v/v) extraction solvent was added to 80 mg sample, and adequately vortexed. For absolute quantification of the metabolites, stock solutions of stable-isotope internal standards were added to the extraction solvent simultaneously. The samples was homogenized by MP homogenizer (24 x 2, 6.0 M/S, 60 s, twice) and sonicated at 4 degrees Celsius (30min/once, twice) then centrifuged at 14,000 g for 20 minutes at 4degrees Celsius and the supernatant was dried in a vacuum centrifuge at 4°C. For LC-MS analysis, the samples were re-dissolved in 100 uL acetonitrile/water (1:1, v/v) solvent and centrifuged at 14000 g at 4degrees Celsius for 15 min, then the supernatant was injected.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14207Xinyan Zhao. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. lily2000222@126.com.Xin Sun.Kexin Si.Heping Wang.Xiang Wei.Yuefei Zhang.Depei Liu. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences. liudp@pumc.edu.cn.Xiaoqiang Tang. West China Second University Hospital, Sichuan University. tangxiaoqiang@scu.edu.cn.Houzao Chen. Institute of Basic Medical Sciences Chinese Academy of Medical Sciences. chenhouzao@ibms.cams.cn.Jingyi Wang. Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences. jywangburn@163.com.Dingsheng Jiang.Huiyu Wang.<p>MultiQuant or Analyst was used for quantitative data processing. The QCs were processed together with the biological samples. Metabolites in QCs with coefficient of variation (CV) less than 30 % were denoted as reproducible measurements.</p>Genotypelily2000222@126.com<p>Acad8flox/flox mice were obtained from the Experimental Animal Tech Co. of Saiye (Beijing, China). The Myh6-Cre (αMHC-MerCreMer) transgenic mice were obtained from Jackson Laboratory. Male C57BL/6 mice (8 weeks old) were obtained from Vital River Laboratory Animal Technology Co., Ltd. Acad8cKO mice were generated by crossing Acad8flox/flox mice with Myh6-Cre (αMHC-MerCreMer) transgenic mice. Conditional cardiomyocyte-specific Acad8 knockout was induced by tamoxifen treatment. Mice (6-8 weeks old, body weight &gt; 22 g) were intraperitoneally administered either vehicle (oil) or tamoxifen (40 mg/kg) every other day for a total of two doses to induce Cre activity. Then, we harvested hearts from both Myh6-Cre and <em>Acad8cKO </em>mice that were subjected to TAC surgery.</p>MetabolomicsHeartMetabolomicsAB SCIEX QTRAP 6500+Mus musculustargeted analysisMousecardiac hypertrophyAgilent 1290 Infinity LCTimepointpooled sampleChinese Academy of Medical Sciences & Peking Union Medical CollegeHeartMetabolomicsAB SCIEX QTRAP 6500+Mus musculustargeted analysisMousecardiac hypertrophyTimepointAgilent 1290 Infinity LCpooled sampleChinese Academy of Medical Sciences & Peking Union Medical College<p>6500+ QTRAP (AB SCIEX) was performed in positive and negative switch mode. The ESI positive source conditions were as follows: Source temperature: 580 degrees Celsius Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain Gas (CUR): 35; IonSpray Voltage (IS): +4500 V; The ESI negative source conditions were as follows: Source temperature: 580 degrees Celsius Ion Source Gas1 (GS1): 45; Ion Source Gas2 (GS2): 60; Curtain gas (CUR): 35; IonSpray Voltage(IS): -4500 V. MRM method was used for mass spectrometry quantitative data acquisition. The MRM ion pairs are showed in the attached file. A polled quality control (QC) samples were set in the sample queue to evaluate the stability and repeatability of the system.</p>Taurodeoxycholic acid (TDCA)Docosanoic AcidCorticosteroneMyristelaidic acidPalmitic acidArachidonic acidProtocatechuic acidKynurenateN-Acetylmethionine2-Pyrocatechuic acidCortexoloneQuinolineIndole-3-methyl acetate5-ValerolactoneElaidic Acid11Z,14Z,17Z-Eicosatrienoic AcidCaffeineMyristoleic acidm-Aminobenzoic acidBenzenebutanoic acidPalmitoylethanolamideIndole-3-lactic AcidFlavone4-Hydroxyhippuric acid4-Hydroxybenzoic acidBeta-Hyodeoxycholic acid (¦Â-HDCA)Gamma-Linolenic acidIsolithocholic acid (IsoLCA)Hyocholic acid (HCA)Sebacic acidCinnavalininate7-Ketolithocholic acid (7-KLCA)PhenolTauro-¦Á-muricholic acid (¦Á-TMCA)7-Dehydrocholic acid (7-DHCA)Retinoic acidTheophylline4-HydroxybenzaldehydeHexanoylcarnitineUndecanoic acid6-MethylthiopurineRicinoleic acidSuberic acidIsodeoxycholic acid (IsoDCA)Methylglutaric acidPyrrole-2-carboxylic acid10Z-Heptadecenoic acidButyrylcarnitineDeoxycholic acid (DCA)Palmitelaidic acid5'-Methylthioadenosine3-MethoxytyramineQuinaldic acidOleic acid10E-Heptadecenoic acidMurideoxycholic acidIndoleacetic acidTaurolithocholic acid (TLCA)Erucic Acid3-Methyladipic acid10Z-Pentadecenoic AcidPentadecanoic acid3-Dehydrocholic acid (3-DHCA)gamma-Glutamyl-phenylalanine5,6-DimethylbenzimidazoleN-AcetylphenylalanineHydroxyphenyllactic acidUrsodeoxycholic acid (UDCA)N-MethylnicotinamideDopamineSerotoninChenodeoxycholic acid £¨CDCA£©Petroselinic acidPhenylacetylglycineLinoelaidic acidp-Cresyl sulfateTauro-¦Â-muricholic acid (¦Â-TMCA)DecanoylcarnitineN-Formyl-methionineLinoleyl carnitineN-AcetylleucineLipoamideSalicylamide13Z,16Z-Docosadienoic AcidLeucylphenylalanineValerylcarnitineTryptamineGlycocholic acid £¨GCA£©10Z-Nonadecaenoic acid12-hydroxystearic acidConjugated linoleic acids (CLA)MelatonineIndole-3-acetamideGlycoursodeoxycholic acid (GUDCA)Glycolithocholic acid-3-SulfateOctanoylcarnitinePhenylalanylalanineVanillic acidTryptophanolCholesterol sulfateIndole-3-carboxaldehydeIndoleacrylic acidStearic acidTetradecanoylcarnitineLeucylalanine3-Phenylbutyric acidPhthalic acidGallic acidOleylcarnitine2-Hydroxycaproic acid4Z,7Z,10Z,13Z,16Z-Docosapentaenoic AcidIndoxyl sulfate2-Hydroxy-3-methylbutyric acidCholic acid (CA)3-MethylindoleBeta-Ursodeoxycholic acid (¦Â-UDCA)Heneicosanoic acidN-AcetyltyrosineMyristic acid2-Hydroxy-2-methylbutyric acidUrsocholic acid (UCA)Phenyllactic acidXanthurenateAdipic acidTaurocholic acid (TCA)5'-DeoxyadenosineVitamin B73-Hydroxybenzoic acidHippuric acidIsovalerylcarnitine3-Aminosalicylic acid7Z,10Z,13Z,16Z-Docosatetraenoic acidPhenylethylamineCaffeateDodecanoic acidBenzamideAlpha-Linolenic Acid4-Hydroxyphenylpyruvic acidDihomo-gamma-linolenic acidAzelaic acidBeta-Muricholic acid (¦Â-MCA)Tridecanoic acid4Z,7Z,10Z,13Z,16Z,19Z-Docosahexaenoic Acid (DHA)3-MethyloxindoleHyodeoxycholic acid £¨HDCA£©Taurochenodeoxycholic acid (TCDCA)11Z-Eicosenoic AcidEpinephrineN-MethyltryptamineLithocholic acid £¨LCA£©Palmitoleic AcidDethiobiotin2',4'-DihydroxyacetophenoneChlorogenic acid5Z,8Z,11Z,14Z,17Z-Eicosapentaenoic Acid2-Hydroxycinnamic acid2-HydroxyphenethylamineLinoleic acidIndole-3-carboxylic acidNordeoxycholic acid (NorDCA)Heptadecanoic acidCitraconic acid1-OleylglycerolVanillinStearylcarnitineLumichromeGlycyl-PhenylalanineGlutamyltryptophanfalseACAD8 deficiency promotes pathological cardiac hypertrophy in response to pressure overload by regulating histone isobutyrylationBranched-chain amino acids play critical roles in cardiac physiology and diseases. Genetic deficiency in the valine catabolic enzyme ACAD8 is clinically associated with isobutyryl-CoA deregulation and cardiomyopathy in humans. To explore the mechanism underlying cardiac pathological hypertrophy in Acad8cKO mice, we performed targeted metabolomic analysis of hearts from both Myh6-Cre and Acad8cKO mice that were subjected to TAC surgery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