MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/m_MTBLS14243_LC-MS_negative_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/m_MTBLS14243_LC-MS_positive_hilic_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/a_MTBLS14243_LC-MS_negative_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/a_MTBLS14243_LC-MS_positive_hilic.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243/s_MTBLS14243.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14243Metabolites were identified by searching in-house, public, and predictive databases, with stringent filtering (score 0.5, QC CV <0.3) and merging of ionization modes (PubChem, HMDB, Metlin, KEGG).MetaboLightsPublicLiquid Chromatography MS - positive - hilicLiquid Chromatography MS - negative - hilicAll samples were for two LC/MS methods. One aliquot was analyzed using positive ion conditions and was eluted from T3 column (Waters ACQUITY Premier HSS T3 Column 1.8 µm, 2.1 mm all_fetch_status all_status eb_eye_copy_status eb_eye_entry_counts eb_eye_fetch_status eb_eye_metabolights_complete.xml eb_eye_metabolights_compounds.copy eb_eye_metabolights_compounds.xml eb_eye_metabolights_studies.copy e_fetch_status europe_PMC_metabolights_studies.copy europe_PMC_metabolights_studies.xml head.xml studies.copy study.xml tail.xml thomsonreuters_metabolights_studies.copy thomsonreuters_metabolights_studies.xml 100 mm) using 0.1 % formic acid in water as solvent A and 0.1 % formic acid in acetonitrile as solvent B in the following gradient: 5 to 20 % in 2 min, increased to 60 % in the following 3 mins, increased to 99 % in 1 min and held for 1.5 min, then come back to 5 % mobile phase B witnin 0.1 min, held for 2.4 min. The analytical conditions were as follows, column temperature, 40 °C; flow rate, 0.4 mL/min; injection volume, 3 μL; Another aliquot was using negative ion conditions and was the same as the elution gradient of positive mode.Cross-Species Mitochondrial Rescue by Gastrodia elata-Derived Extracellular Vesicles Protects the Stressed Mammalian Heart.Ziyi YinHuazhong Agricultural Universityheartmass spectrometry assayThe sample stored at -80 °C refrigerator was thawed on ice. The thawed sample was homogenized by a grinder (30 HZ) for 20 s. A 400 μL solution (Methanol : Water = 7:3, V/V) containing internal standard was added in to 20 mg grinded sample, and shaked at 1500 rpm for 5 min. After placing on ice for 15 min, the sample was centrifuged at 12000 rpm for 10 min (4 °C). A 300 μL of supernatant was collected and placed in -20 °C for 30 min. The sample was then centrifuged at 12000 rpm for 3 min (4 °C). A 200 μL aliquots of supernatant were transferred for LC-MS analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14243Min Zhang. College of Biomedicine and Health, College of Life science and Technology, Huazhong Agricultural University,. minzhang@mail.hzau.edu.cn.Ziyi Yin. College of Biomedicine and Health, College of Life science and Technology, Huazhong Agricultural University. 2411612041@qq.com.Raw MS data were converted to mzML format using ProteoWizard and processed by XCMS for peak extraction and alignment. Missing values were imputed using KNN or 1/5 minimum value strategies, followed by SVR-based peak area correction.PCA: Unsupervised PCA (principal component analysis) was performed by statistics function prcomp within R (www.r-project.org). The data were processed by UV (unit variance scaling) before PCA.Hierarchical Cluster Analysis and Pearson Correlation Coefficients: The HCA (hierarchical cluster analysis) results of samples and metabolites were presented as heatmaps with dendrograms, while pearson correlation coefficients (PCC) between samples were calculated by the cor function in R and presented as only heatmaps. Both HCA and PCC were carried out by R package ComplexHeatmap. For HCA, normalized signal intensities of metabolites (unit variance scaling) are visualized as a color spectrum.Differential metabolites selected: For two-group analysis, differential metabolites were determined by VIP (VIP > 1) and P-value (P-value < 0.05, Student's t test). For multi-group analysis, differential metabolites were determined by VIP (VIP > 1) and P-value (P-value < 0.05, ANOVA). VIP values were extracted from OPLS-DA result, which also contain score plots and permutation plots, was generated using R package MetaboAnalystR. The data were processed by log2 transformation and Zero-centered before OPLS-DA. In order to avoid overfitting, a permutation test (200 permutations) was performed.KEGG annotation and enrichment analysis: Identified metabolites were annotated using KEGG Compound database (http://www.kegg.jp/kegg/compound/), annotated metabolites were then mapped to KEGG Pathway database (http://www.kegg.jp/kegg/pathway.html).Group2411612041@qq.com2025.11: A/A Mice were pre-treated with epinephrine and subjected to 21 days of social isolation (SI) or group housing (GH). GE-EVs or PBS (Ctrl) were administered every 3 days. Fresh hearts were excised, snap-frozen in liquid nitrogen, and stored at -80°C.MetabolomicsThermo Scientific Vanquish UHPLC SystemMetabolomicsMus musculusHeart tissueuntargeted metabolomics70-1000untargeted analysisThermo Scientific Q Exactive HF-Xmzmineheartcardiovascular disorderThermo Scientific Vanquish UHPLC SystemMetabolomicsMus musculusHeart tissueuntargeted metabolomics70-1000untargeted analysisThermo Scientific Q Exactive HF-Xmzmineheartcardiovascular disorderAll the methods alternated between full scan MS and data dependent MSn scans using dynamic exclusion. MS analyses were carried out using electrospray ionization in the positive ion mode and negative ion mode using full scan analysis over m/z 70-1000 at 60000 resolution. Additional MS settings are: ion spray voltage, 3.8 KV or 3.4 KV in positive or negative modes, respevtively; Sheath gas (Arb), 60; Aux gas, 20; Ion transfer tube temperature, 320 °C; Vaporizer temperature, 350 °C; Collision energy, 30,40,50 V; Top N vs Top speed, 10.6-Phosphogluconic-acidSuccinyl-CoALactateSuccinic-AcidGuanosineFructose-1,6-bisphosphatec-di-AMPOxaloacetateGlucuronic-acidDL-Glyceric-AcidADPNicotinaMide-adenine-dinucleotide(NAD)GluconatePhosphoenolpyruvic-acidL-AlanineD-Ribose-5-phosphate-disodiumItaconic-acid2-Phospho-D-glycerateAlpha-Ketoglutaric-Acid2-Oxoadipic-acidTyrosineL-LeucineAMPL-CystineThreonineFlavin-mononucleotideD-Erythrose-4-phosphateD-Fructose-6-phosphate3-phenyllactic-acidGlyceraldehyde-3-phosphateIMPD-Ribulose-5-phosphateArgininosuccinic-acidPhosphorylethanolamineLysineFumaric-acidL-Glutamic-acidUMPD-Glucose-1-phosphateOrnithinedTMPTrehalose-6-phosphateCitric-acidD-Glucose-6-phosphateSerineL-2-Hydroxyglutaric-acid-disodiumCyclic-AMPL-AsparagineDihydroxyacetone-phosphateUDP-GlcNAcMalic-acidD(+)-GlucoseL-citrullineGlycerol-3-phosphateArginineD-ribulose-1,5-bisphosphate2,3-DPGIsocitric-acidGlycolic-acidPyruvic-acidSedoheptulose-7-phosphatecis-Aconitic-acidAdenineAcetyl-CoAdCMPD-Mannose-6-phosphatedAMPCysteic-acidXylulose-5-phosphateTriphosphate-guanosine3-phosphoglycerateInosinedUMPDihydronicotinamide-adenine-dinucleotide-phosphate(NADPH)ATPUracilUreidopropionateL-AspartateGuanosine-diphosphateGlutaminefalseCross-Species Mitochondrial Rescue by Gastrodia elata-Derived Extracellular Vesicles Protects the Stressed Mammalian HeartIn this study, we developed Gastrodia elata (G. elata)-derived extracellular vesicles (GE-EVs) to achieve functional rescue of the stressed mammalian heart. Using RBM24 phosphorylation-deficient (S181A, A/A) mice, we demonstrated that GE-EVs have a profound cross-species rescue effect on mitochondria. GE-EVs therapy restores mitochondrial integrity and ATP synthesis through mitochondrial quality control, thereby reversing myocardial hypoxia and attenuating ROS production.2026-04-092026-04-09MTBLS14243