MetaboLightsapplication/xmlftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/m_MTBLS14254_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/m_MTBLS14254_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/a_MTBLS14254_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/a_MTBLS14254_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254/s_MTBLS14254.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14254<p>Metabolite feature identification and annotation were performed using Mzmine3 together with reference databases including HMDB, MassBank, and MetFrag. Significant metabolites were further interpreted using pathway-level analyses in MetaboAnalyst and KEGG-based analyses as described in the vendor reports.</p>MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase<p>Chromatographic separation was performed using an ACQUITY UPLC system (Waters) fitted with an ACQUITY UPLC HSS T3 column (100 × 2.1 mm, 1.8 µm; Waters). The mobile phase consisted of solvent A, 0.05% formic acid in water, and solvent B, acetonitrile. Gradient elution was as follows: 0-1 min, 5% B; 1-12 min, 5%-95% B; 12-13.5 min, 95% B; 13.5-13.6 min, 95%-5% B; 13.6-16 min, 5% B. The flow rate was 0.3 mL/min. Column temperature was maintained at 40°C and the sample manager temperature was set to 4°C.</p>Inflammatory cytokines induce novel cancer dependencies.Collins collins@broadinstitute.orgBroad Institutecell culturemass spectrometry assay<p>Samples were thawed and extracted by adding 800 µL of 80% methanol to each tube. Samples were vortexed for 60 s, sonicated for 30 min at 4°C, incubated at -20°C for 1 h, and centrifuged at 12000 rpm for 15 min at 4°C. A 200 µL aliquot of supernatant was transferred to an LC-MS vial, and 5 µL of DL-o-Chlorophenylalanine (0.14 mg/mL) was added prior to analysis. Pooled quality control (QC) samples were prepared by mixing equal amounts of extract from each sample and processing them using the same preparation procedure as the experimental samples.</p>Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14254Collins Cheruiyot. Broad Institute. 300 Binney St., Cambridge, MA 02141. collins_cheruiyot@g.harvard.edu.<p>Raw data were acquired and aligned using Compound Discoverer 3.0 (Thermo) based on ion m/z values and retention times. Features detected in ESI+ and ESI− modes were merged and imported into SIMCA-P version 14.1 for multivariate statistical analysis. Principal component analysis (PCA) was used for unsupervised visualization and outlier assessment, followed by partial least squares discriminant analysis (PLS-DA) or orthogonal PLS-DA (OPLS-DA) for supervised modeling. Biomarkers were filtered using VIP values greater than 1.5 together with t-test p values less than 0.05.</p>TreatmentGenotypecollins_cheruiyot@g.harvard.edu<p>Murine KPC tumor cell pellets were used for untargeted metabolomics analysis. KPC control and FITM2 knockout cells were cultured in vitro and treated under the indicated experimental conditions, including PBS or IFNγ treatment. For the submitted study, 24 total samples were analyzed, corresponding to 4 conditions with 6 biological replicates each. After treatment, samples were collected as cell pellets, flash-frozen, and stored frozen prior to shipment for LC-MS analysis. Sample type was recorded by the vendor as murine cell pellet.</p>MetabolomicsMetabolomicsMus musculuscompound treatment designThermo Scientific Q Exactivecell culturestudy reference materialuntargeted analysisWaters ACQUITY UPLC systemgenotype designuntargeted metabolite profilingexperimental sampleMetabolomicsMus musculuscompound treatment designThermo Scientific Q Exactivecell culturestudy reference materialuntargeted analysisWaters ACQUITY UPLC systemgenotype designuntargeted metabolite profilingexperimental sample<p>Mass spectrometric detection was performed on a Q Exactive MS system (Thermo) with electrospray ionization in positive mode. Source settings for ESI+ were: heater temperature 300°C, sheath gas flow rate 45 arbitrary units, auxiliary gas flow rate 15 arbitrary units, sweep gas flow rate 1 arbitrary unit, spray voltage 3.0 kV, capillary temperature 350°C, and S-Lens RF level 30%. </p>MG(20:5/0:0/0:0)LysoPE(0:0/18:0)GlycerylphosphorylethanolamineLysoPE(20:2/0:0)LysoPE(22:2/0:0)2-Aminonicotinic acid5-Octadecynoic acidPalmitic acidMG(P-18:0/0:0/0:0)Vitamin AHistidineLysoPE(0:0/20:5)Prolinamide4-AminophenolDG(20:5-3OH(5,6,15)/0:0/20:0)Prolyl-AsparagineOctadeca-9,11,13-trienoic acidThreonine2-DodeceneGlycyl-Cysteine4-OH-Retinal6-Hydroxyhexanoic acidMG(0:0/20:3/0:0)Glyceryl monolinoleate6-HydroxyphenylpropionylglycineStearaldehydeLysoPC(22:6/0:0)LysoPE(20:4/0:0)LysoPC(20:3/0:0)2-Hydroxyundec-7-enoylcarnitineDihydrouracilLysineSitostanol-betaGlycerophosphocholine4-Hydroxybenzaldehyde4-HydroxyprolineMG(18:3/0:0/0:0)N-Carbamoyl-2-amino-2-(4-hydroxyphenyl)acetic acidLysoPE(0:0/16:0)4-AcetylbutyrateHomolanthionineCarnosine3-(3-Amino-3-carboxypropyl)uridinePhe-Pro-IleLysoPE(16:1/0:0)CystinePyrimidineLysoPE(18:2/0:0)OctadecanamideMevalonic acidArginineLinoleamide11,12-Dihydroxy-7,14-dimethoxy-8,11,13-abietatrien-20,6-olidePyroglutamic acidLysoPC(20:2/0:0)2'-HydroxyacetanilideLysoPA(P-16:0/0:0)Decyl alcoholGlutathioneNorspermidineCreatinineNiacinamideLysoPC(18:3/0:0)6-MethylquinolinePhenylalanine2-Chloro-1-propanolLysoPE(22:6/0:0)4-Oxo-proline5-HydroxylysineLysoPE(0:0/18:3)3-Hydroxyundecanoyl carnitineLycopersiconolLysoPC(14:0/0:0)LysoPC(18:0/0:0)7-MethylguanineCPA(18:0/0:0)Diethyl oxalpropionate1-TrideceneArachidyl carnitineN-Myristoyl Aspartic acidCholineOleamideGalabiosylceramide (d18:1/16:0)DimethylarginineAsparaginePalmitaldehydeUracilGyromitrinGlycerol 3-phosphateNorleucineGuanosine3-Methylbutyl formateCPA(18:1/0:0)LysoPC(0:0/16:0)3-Mercaptopyruvic acid3-Palmitoyl-sn-glycerolLysoPE(0:0/18:4)Lithocholyltaurine5-AndrostenetriolIndoleacetaldehydeLysoPC(18:2/0:0)Malic acidPersicaxanthin4-hydroxysphinganineCytosineGlutamylleucine24-Propylcholestan-3-olPalmoxiric acidEthosuximidePterin6-MethylcoumarinN,N-Dimethylsphingosine2-Hydroxy-3-methylbutyric acidGuanidinosuccinic acidPhosphorylcholinePalmitoylcarnitine2-Hydroxy-3-methylpentanoic acid1-HeptadeceneCarnitineHistamine1,3-OctadieneLysoPC(16:1/0:0)LysoPC(18:1/0:0)LysoPE(P-18:1/0:0)Hydroxypropionic acidHypoxanthineMG(O-18:0/0:0/0:0)11-HexadecenalOrnithineAdipic acidN-Palmitoyltaurine1,2,4-NonadecanetriolIsovalerylglycineAll-trans-13,14-dihydroretinolIndoleSerineProline1-Phenyl-1-pentanone4-Nitrophenyl acetateLysoPE(18:1/0:0)Glyoxylic acidLysoPE(P-18:0/0:0)LysoPA(0:0/18:2)SM(d18:1/16:0)Undecylenic acidAnhydroretinolDodec-9-enedioylcarnitineLysoPE(0:0/20:1)LysoPC(20:1/0:0)GlycerophosphoinositolAdenineAcetyl citrateMethylcarbamodithioic acidCyclohexanecarboxylic acidCPA(16:0/0:0)PregnanetriolLysoPE(20:3/0:0)Norvaline1-HydroxyisoquinolineInosinePurine3-Hydroxyphenylacetic acidGlutaminefalseUntargeted metabolomics profiling to determine FITM2-dependent metabolic rewiring under IFNγ stimulationThis study aimed to define metabolic alterations associated with FITM2 loss in tumor cells exposed to inflammatory cytokine signaling. KPC control and FITM2 knockout cells were treated with IFNγ and subjected to untargeted metabolomics profiling using LC–MS/MS. Data from positive and negative ionization modes were integrated to generate a unified metabolite dataset. Differential abundance analysis was performed to identify metabolites significantly altered between conditions, and pathway enrichment analysis was conducted to uncover metabolic pathways perturbed by FITM2 deficiency under IFNγ stimulation.2026-04-112026-04-11MTBLS14254HMDB0000131HMDB0002217HMDB0002113HMDB0000251HMDB0012228HMDB0002104HMDB0001844HMDB0000525HMDB0000191HMDB0000156HMDB0000943HMDB0000641HMDB0041683HMDB0000710HMDB0000336HMDB0000020HMDB0001190HMDB0304424HMDB0005175HMDB0000500HMDB0029739HMDB0000893HMDB0000517HMDB0001209HMDB0000707HMDB0000158HMDB0000118HMDB0041600HMDB0062181HMDB0001370HMDB0011745HMDB0001874HMDB0000127HMDB0000621HMDB0340971HMDB0060618HMDB0243595HMDB0002096HMDB0062176HMDB0000929HMDB0000881HMDB0030402HMDB0000684HMDB0012149HMDB0010727HMDB0252811HMDB0000210HMDB0001104HMDB0246742HMDB0000806HMDB0001982HMDB0028781HMDB0062175HMDB0060649HMDB0000192HMDB0000826HMDB0000296HMDB0000767HMDB0028757HMDB0000220HMDB0000853HMDB0004813HMDB0011171HMDB0004821HMDB0060038HMDB0302278HMDB0246178HMDB0112186HMDB0001388HMDB0028991HMDB0000706HMDB0012535HMDB0000673HMDB0028988HMDB0061661HMDB0000133HMDB0000299HMDB0000827HMDB0005923HMDB0000343HMDB0031711HMDB0028882HMDB0029156HMDB0031103HMDB0244806HMDB0013622HMDB0001563HMDB0030979HMDB0255539HMDB0001043HMDB0060039HMDB0000125HMDB0001530HMDB0005060HMDB0028831HMDB0002231HMDB0033361HMDB0034382HMDB0002212HMDB0028755HMDB0002656HMDB0252073HMDB0253021HMDB0245974HMDB0062725HMDB0028830HMDB0061714HMDB0253281HMDB0012788HMDB0002664HMDB0001403HMDB0244553HMDB0033372HMDB0006590HMDB0034475HMDB0001397HMDB0247077HMDB0002183HMDB0029799HMDB0254974HMDB0260490HMDB0014495HMDB0011534HMDB0061664HMDB0011533HMDB0039733HMDB0003034HMDB0011539HMDB0242007HMDB0060044HMDB0059736HMDB0038423HMDB0092902HMDB0304947HMDB0007007HMDB0011545HMDB0011544HMDB0011550HMDB0011144HMDB0000277HMDB0011503HMDB0011152HMDB0011504HMDB0240599HMDB0010380HMDB0011508HMDB0011507HMDB0003080HMDB0007851HMDB0011519HMDB0011517HMDB0011514HMDB0011513HMDB0011512HMDB0012108HMDB0062305HMDB0010379HMDB0011510HMDB0011479HMDB0011496HMDB0011525HMDB0243890HMDB0093047HMDB0242388HMDB0010383HMDB0011489HMDB0007077HMDB0112110HMDB0010387HMDB0010386HMDB0000286HMDB0010385HMDB0041032HMDB0061695HMDB0007041HMDB0112117HMDB0112152HMDB0010395HMDB0010393HMDB0010392HMDB0259130HMDB0061693HMDB0007097HMDB0000290HMDB0288846HMDB0295507HMDB0112109HMDB0252757HMDB0295595HMDB0295927HMDB0299640HMDB0007862HMDB0272111HMDB0000167HMDB0000700HMDB0031335HMDB0033952HMDB0034163HMDB0003361HMDB0000097HMDB0000187HMDB0001169HMDB0000630HMDB0000870HMDB0000300HMDB0000562HMDB0000076HMDB0253910HMDB0000119HMDB0000162HMDB0000738HMDB0013716HMDB0000407HMDB0001366HMDB0011718HMDB0039221HMDB0246561HMDB0000267HMDB0061881HMDB0000725HMDB0001645HMDB0000168HMDB0000317HMDB0012843HMDB0000214HMDB0000034HMDB0000157HMDB0001406HMDB0033115HMDB0243900HMDB0000448HMDB0000182HMDB0031342HMDB0061919HMDB0011634HMDB0000177HMDB0000678HMDB0001368HMDB0000062HMDB0000450HMDB0031208HMDB0000802HMDB0000897HMDB0000159HMDB0000126HMDB0000440HMDB0003157HMDB0011624HMDB0001565HMDB0033724HMDB0061819HMDB0028838HMDB0251395HMDB0030930HMDB0000114HMDB0000033HMDB0029012HMDB0031813HMDB0040966HMDB0032306HMDB0059808HMDB0000086HMDB0028823HMDB0243751HMDB0302312HMDB0001551HMDB0000195HMDB0062447HMDB0340921HMDB0062656HMDB0031097HMDB0002117HMDB0034146HMDB0256092HMDB0000305HMDB0011618HMDB0002034HMDB0002384HMDB0304734HMDB0011649HMDB0029583HMDB0006070HMDB0032112HMDB0011570HMDB0242115HMDB0241170HMDB0061637HMDB0011153HMDB0011143HMDB0013645HMDB0011580HMDB0034952HMDB0242130HMDB0241226HMDB0031933HMDB0007003HMDB0011154HMDB0000222HMDB0304808HMDB0007005HMDB0007004HMDB0094727HMDB0011473HMDB0006460HMDB0007852HMDB0240598HMDB0011478HMDB0011505HMDB0011129HMDB0034814HMDB0011480HMDB0240262HMDB0000722HMDB0011515HMDB0011482HMDB0010384HMDB0011526HMDB0010391HMDB0010404HMDB0011522HMDB0296374HMDB0004833HMDB0010169HMDB0240594HMDB0242048HMDB0245964HMDB0304183HMDB0000550HMDB0246538HMDB0032394HMDB0014731HMDB0061680HMDB0000227