Metabolite identification was performed based on the Metware database (MWDB), using information from retention time, precursor ion (Q1), product ion (Q3), and fragmentation patterns obtained by mass spectrometry.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["Metabolite separation was performed on a Agilent SB-C18 column (1.8 um, 2.1 x 100 mm). The mobile phase consisted of water with 0.1% formic acid (A) and acetonitrile with 0.1% formic acid (B). The gradient program was as follows: 0 min, 5% B; 0-9 min, 5-95% B; 9 min, 95% B; 9-10 min, 95% B; 10-11.1 min, 5% B, 11.1-14 min, 5% B. The flow rate was 0.35 mL/min, the column temperature was set at 40 C, and the injection volume was 2 uL.
"],"publication":["Bio-organic fertilizer alleviates tobacco continuous cropping obstacles by coordinately reprogramming plant metabolism and the rhizosphere microbiome."],"submitter_affiliation":["zhejiang university"],"submitter_name":["Dan Chen"],"organism_part":["Root","soil"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Metabolites in rhizosphere soil were extracted with deionized water (w: v = 1: 5) by oscillation for 3 h. After centrifugation at 8000 rpm for 5 min, the supernatant was filtered through 0.22 uM filters, freeze-dried, and reconstituted with 70% methanol. The extracts were vortexed, sonicated in an ice-water bath, centrifuged (12,000 rpm, 3 min, 4 C), and filtered prior to UPLC-MS/MS analysis. For tobacco roots, 50 mg of freeze-dried powder was extracted with 1 mL of 70% methanol and 500 uL petroleum ether, followed by vortexing and centrifugation (12,000 rpm, 10 min, 4 C). The supernatant was filtered (0.22 um, PTFE) before UPLC-MS/MS analysis. Widely targeted metabolomic analyses were conducted by Wuhan Meiwei Metabolic Biotechnology Co., Ltd. (Wuhan, China). Quality control (QC) samples were prepared by pooling extracts from all samples to evaluate the reproducibility of the analytical process, and one QC sample was injected after every ten samples.
"],"organism":["Nicotiana tabacum"],"data_transformation_protocol":["The raw metabolite data were processed by peak area integration using Analyst software. The resulting data matrix was normalized to reduce systematic variations. Subsequently, log2 transformation was applied to improve data normality and reduce heteroscedasticity. The data were further scaled using unit variance (UV) scaling prior to multivariate statistical analysis.
"],"study_factor":["Treatment"],"metabolights_link":["https://www.ebi.ac.uk/metabolights/MTBLS14267"],"submitter_email":["dancyjz@zju.edu.cn"],"sample_collection_protocol":["Three fertilizer treatments were designed in a rhizobox experiment, each with four replicates: (1) CF, chemical fertilizer (N: P2O5: K2O = 12: 6: 24); (2) OF, combination of chemical and organic fertilizers; and (3) BF, combination of chemical and bio-organic fertilizers. Tobacco seedlings were cultivated in an artificial climate chamber and irrigated with deionized water every 3 days. At 30 and 60 days, approximately 50 g of rhizosphere soil was collected from the middle layer of each rhizobox. Tobacco roots were carefully washed with tap water followed by deionized water to remove adhering soil, and then frozen in liquid nitrogen and stored at ultra-low temperature freezer.
"],"omics_type":["Metabolomics"],"study_design":["ultra-performance liquid chromatography-mass spectrometry","Multi-omics study","Metabolomics","SCIEX ExionLC AD","Root","targeted analysis","AB SCIEX QTRAP 4500","soil","targeted metabolite profiling","Nicotiana tabacum","microbiome"],"curator_keywords":["ultra-performance liquid chromatography-mass spectrometry","Multi-omics study","Metabolomics","SCIEX ExionLC AD","Root","targeted analysis","AB SCIEX QTRAP 4500","soil","targeted metabolite profiling","Nicotiana tabacum","microbiome"],"mass_spectrometry_protocol":["Mass spectrometry analysis was performed using a triple quadrupole-linear ion trap mass spectrometer (QTRAP, AB Sciex) equipped with an electrospray ionization (ESI) source. The instrument was operated in both positive and negative ion modes. The ESI source parameters were set as follows: source temperature 500 C; ion spray voltage 5500 V (positive) and -4500 V (negative); curtain gas 25 psi; ion source gas I and gas II were set to 50 and 60 psi, respectively. Metabolites were detected using scheduled multiple reaction monitoring (MRM) mode. Declustering potential (DP) and collision energy (CE) were optimized for individual MRM transitions.
"],"metabolite_name":["Coniferaldehyde","Lys-Glu-Ser","N-acetyltyramine1-O-beta-glucoside","1-Feruloyl-sn-glycerol","Methyl linolenate","Phe-Ile","Murrayacarpin A glucoside","Dehydronerolisovalerate","Asp-Glu-Leu","Cholest-5-en-3-ol (Cholesterol)","Lys-Asp*","Leu-Glu-Ile","Prolylproline","1(10)E,4E-Germacrone","1-Linolenoylglycerol","linoleoyl ethanolamine","Eugenyl formate","Crotonoside; 2-Hydroxyadenosine","1-Oleoyl-Sn-Glycerol","LysoPE 17:1(2n isomer)*","N-Feruloyloctopamine*","Cis-Zeatin-7-N-glucoside*","Isoguanine","NG,NG-Dimethyl-L-arginine*","(6-amino-3-oxohexan-2-yl)proline","Asn-Ile","Calystegine","3,5-di-tert-Butyl-4-hydroxyacetophenone","Bis(Caffeoyl)Spermidine","Val-Tyr-Asp","N6-Acetyl-L-lysine","(1-Methylpyrrolidin-2-yl)aceticacid*","Symplocosin*","7,8-Dihydroxy-4-methylcoumarin","Isopentenyladenine9-Glucoside","Isogermafurenolide*","N-(1-Deoxy-1-fructosyl)Tyrosine","LysoPE 15:1*","L-Homomethionine","L-Theanine","Pro-Asp-Lys","2-Aminotetradecane-1,5,13-triol","2-Keto-1(5)-¦Â-patchoulene-4¦Â-ol","Hemileptaglin","6-Hydroxykynurenic acid*","7-hydroxy-N-trans-ferulic tyramine","Methylephedrine","4-Methyl-5-thiazoleethanol","L-Valyl-L-Leucine","11S-nootkatone-11,12-diol","N5-(4-Methoxybenzyl)glutamine","N-Methyl-L-proline","Costal","1-Linoleoylglycerol-2,3-di-O-glucoside*","2-AminoicoSane-1,5,19-triol","7-methoxy-1-[(4-methoxyphenyl)methyl]-2-methyl-3,4-dihydro-1h-isoquinolin-6-ol","Phe-Gln","Hydroxythreonine xyloside","N-Nonanoyl nornicotine","L-Ornithine","1-Hydroxyanthraquinone","LysoPC 16:2*","10-Hydroxymethyllycaconitine","Succinyladenosine","hex-2-enylbenzoate","LysoPE 17:1*","Damascenone","2-Methoxy-6-undecyl-1,4-benzoquinone","LysoPC 19:1","Phe-Glu","Scopoletin 7-O-¦Â-D-Sophoroside*","Pro-Val-Leu","Methyl Hydroxycinnamate","7-Methoxy-3-[1-(3-pyridyl)methylidene]-4-chromanone","1-O-Galloyl-¦Â-D-glucose","1¦Â-Hydroxy-¦Á-cyperone","D-Glucosyl Indole-3-Carboxylate","Elemicin","Lys-His","2,6-diamino-7-methyl-5-oxooctanoic acid","Allyl salicylate","Ser-Val-Val","Senkyunolide C*","L-Glutaminyl-L-isoleucine","1-(Dihydroxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-(5-8)-dimethoxy-1,2dihydronaphthalene-2,3-dicarboxamide","N-Feruloylphenylalanine*","Butyl beta-D-glucoside","Thr-Val-Phe","Indole","Gly-Tyr*","2-aminodocoSane-1,6,19,20,21-pentaol","Octadeca-11E,13E,15Z-trienoic acid","Isoleucyl-phenylalanyl-threonine","Benzamide","L-Prolyl-L-Phenylalanine","Phe-Thr","Dibutylamine","Lycoperodine-1","L-Carnitine","2-Aminotetradecane-1,11,13-triol","Cyclo(Pro-Leu)","Met-Thr","Loliolide","10-Dehydrogeniposide","Allopurinol","Sinapaldehyde-4-O-Glucoside","Isololiolide","Isofraxidin-7-O-glucoside*","Glu-Thr-Leu","Precocene II","Matairesinol","LysoPC 20:2(2n isomer)","Methyleugenol","N-Feruloyltyramine; Moupinamide*","Leu-Val-Val","Phenyl salicylate","3-pyridine-methanol-O-¦Â-D-glucopyranosyl","Benzoic acid","L-Leucyl-L-Leucine","L-Phenylalanine","4-Hydroxy-8-sphingenine","Riboprine","N-Acetyl-D-galactosamine","N-Methylpyrrolidone","Zanthoxyloside*","11,12-cis-13,14-Dihydroretinol","LysoPC 17:1","DIMBOA glucoside","3-Ethylidene-7-hydroxy-6-methoxyphthalide","D-Pantothenic Acid 4'-O-Beta-Glucoside","7-oxo-11-Dodecenoic acid","N-trans-feruloyl-3'-O-methyl dopamine*","Glutinosone","2-Aminohexadecane-1,5,15-triol","2-Aminotetradecan-1-ol*","Tyrosylleucine","DL-Tryptophan","L-Prolyl-L-Leucine","5-hydroxy-7-(1',2'-dihydroxypropyl)-2-methyl-chromone","N',N'',N'''-p-Coumaroyl-cinnamoyl-caffeoyl spermidine","Lys-Asn","3,4-dihydroxyallylbenzene","Guanine","pithecellobiumol B","O-Acetyl-L-carnitine","Valyl-alanyl-phenylalanine","5,8-Dihydroxy-2-[2-(4'-methoxyphenethyl)]chromone","2,6-Dimethoxy-1,4-benzoquinone*","Pro-Val-Gly","N-Formyl nornicotine","LysoPC 15:1","8-hydroxyquinoline","Phenylethyl Primeveroside","LysoPE 20:3(2n isomer)*","D-Threose","LysoPC 16:1*","N-Acetyl-L-Arginine","Cyclo(Pro-Pro)","1-Methyladenine","Val-Ile-Asp","7-Oxodehydroabietic acid","N-Isobutyl Decanamide","3-Oxo-Alpha-Ionol 3'-(6''-Malonyl)Glucoside","LysoPE 15:1(2n isomer)*","2-(Acetylamino)-3-phenyl-2-propenoic acid","Asn-Ile-Asp","14,15-Dehydrocrepenynic acid","Pipecolic acid","N-Octanoyl nornicotine","p-Coumaroylputrescine","Leu-Ala-Ile*","Octadeca-9,12,15-trienoic acid","Indole-3-carboxaldehyde","Punicic acid (9Z,11E,13Z-octadecatrienoic acid)","Pro-Thr-Ile","LysoPE 16:1(2n isomer)*","Furanodienone","Fraxidin (8-Hydroxy-6,7-dimethoxycoumarin)","N-benzylformamide*","Met-Phe","Thr-Phe-Ser","Nicotinic acid (Vitamin B3)","Scopoletin 7-O-(6''-Malonylglucoside)","Diisobutyl phthalate*","Lys-Tyr","LysoPE 17:0","N-trans-coumarinyl tyramine*","LysoPE 15:0*","13(s)-hydroperoxy-(9z,11e,15z)-octadecatrienoic acid","Methylillicinone F","Gly-Val-Ala","Acropyrone","Menatetrenone (Vitamin K2)","N-Feruloylphenylacetamide glucoside","LysoPE 18:1(2n isomer)*","L-Isoleucine*","LysoPE 18:2(2n isomer)","2-Linoleoylglycerol-1,3-di-O-glucoside*","3¦Â-Hydroxyatractylon*","2-Amino-4,5-dihydro-1H-imidazole-4-acetic acid","Riboflavin (Vitamin B2)","Spathulenol","9-Arabinosyladenine*","Dimethylfraxetin; 6,7,8-Trimethoxycoumarin*","N-trans-feruloyl-3-methoxytyramine*","Gramine","¦Ã-Aminobutyric acid","3,5-Dimethyl-2,3-dihydrobenzofuran","Fagaramide","Dihydroisopelletierine","Glycerol 9,11,13-octadecatrienoyl ester*","Trp-Thr","Ser-Ser-Gly","Gly-Val-Phe","Quercetin-3-O-glucoside (Isoquercitrin)","N(6),N(6)-Dimethyl-L-lysine","9-Oxo-10,12-Octadecadienoic Acid","Pinolenic acid","3-Indoleacrylic acid*","naphthisoxazol A*","Gly-Glu-Leu","Ergosta-5,24(28)-diene-3,20,26-triol (Podecdysone C)*","Cinnamoyltyramine","Asp-Ile-Asp","N'-Formylkynurenine","3-Hydroxyurs-12,19-dien-28-oic acid (Sanguisorbigenin)","Dehydrodiconiferyl alcohol-gamma'-O-glucoside*","4-Hydroxysphinganine; Phytosphingosine","12(13)Ep-9-KODE","Ala-Phe-Thr","Gln-Gly","Retronecine","Agmatine","2-aminodocoSane-1,5,7,21-tetraol","Trigonelline","Dicumarol","Leu-Ile-Glu","Isovanillin","6,8-Dihydroxy-2-[2-(4-methoxyphenyl)ethyl]chromone","Cryptomerione","LysoPC 19:2(2n isomer)","2-g-Linoleoyl-glycerol*","Butyl isobutyl phthalate*","Zarzissine","Isobavachalcone glucoside","Isofuranodiene","cis-Zeatin-O-glucoside*","5-(2-hydroxypropyl)-3H-2-benzofuran-1-one","4-(2-aminoethenyl)phenol*","Dibutyl phthalate*","Hydroxycotinine*","L-Alanyl-L-tryptophan","1-ethyl-3-hydroxy-5-(pyridin-3-yl)pyrrolidin-2-one","LysoPE 20:4*","N-Feruloyltyramine 4'-glucoside","Neoligustilide","Ile-Gly-Ile","trans-3'-Hydroxycotinine-O-glucoside","Leu-Glu-Phe","Indole-3-acetic acid (IAA)","Oleamide (9-Octadecenamide)","Octadec-2-enamide","Curcumol","2-aminohexadec-4-ene-1,3-diol","Pro-Leu-Thr","4-hydroxy-L-Isoleucine","balanophon n","N-Feruloyltyramine 4-glucoside","p-Coumaroylspermidine","Ala-Ile-Ala","Tyr-Ala","Caffeoyl-dihydrocaffeoyl spermidine","L-Valine","Myosmine","9-O-¦Â-D-Glucoside of 3-hydroxy-7,8-didehydro-¦Â-ionol*","Vidarabine","1-¦Á-Linolenoyl-glycerol*","N5-(1-Iminoethyl)-L-ornithine","Phloroglucinol","4-Hydroxypyridine","Kaempferol-3-O-glucoside (Astragalin)*","1-acetyl-5-(pyridin-3-yl)-3,4-dihydro-2H-pyrrol-1-ium","Pseudosinin M","Nootkatone*","3-Chloroaniline","DL-isoleucyl-DL-isoleucyl-DL-isoleucine","Cytosine","Beauvericin","solajiangxins H","N6-Methyl-2'-deoxyadenosine","7-epi-teucrenone","3-O-Glucoside-3-hydroxy-y-butyrolactone","Kaempferol-4'-O-glucoside*","3-Succinoylpyridine","Ser-Gly-Ile","N-trans-ferulic tyramine*","7-Hydroxy-1-(3,4-dihydroxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-6,8-dimethoxy-1,2-dihydronaphthalene-2,3-dicarboxamide","LysoPE 18:3(2n isomer)","Val-Leu-Gln","Canagaterpene III","Fraxetin glucose glucoside","2-AminoicoSane-1,6,18,19,20-pentaol","N-Cis-Feruloyl-3'-O-methyldopamine","2¦Â-Methoxy-14-nor-¦Â-patchoulane-1(5)-ene-4-one","Nicotine","Trp-Phe","Leu-Phe-Asp","N-[2-(3,4-Dihydroxyphenyl)-2-hydroxyethyl]-3-(4-methoxyphenyl)-2-propenamide*","N-(beta-D-Glucosyl)nicotinate","¦Á-Ionone","Met-Tyr","Phe-Gln-Ser","N-Butanoyl nornicotine*","L-Tyramine","N-acetyl-nornicotine","Isoscopoletin-¦Â-D-glucoside","Fraxetin-8-O-glucoside (Fraxin)","Met-Asn","Gly-Leu-Asp","L-Seryl-L-Isoleucine","L-Homocystine","Leu-Asp-Gln","Avicennol*","LysoPE 16:0(2n isomer)","L-Proline*","Myricatomentogenin","Ditartaroyl-hydroxycoumarin","5,6,7-Trimethoxycoumarin*","Ala-Ile-Leu","3-(3,4-Dihydroxyphenyl)-N-[2-(4-hydroxyphenyl)ethyl]prop-2-enamide*","Ile-Gln-Leu","Atractylenolide ¢ò*","LysoPC 20:5","LysoPC 20:4","LysoPC 20:3","LysoPC 20:2","6-Aminocaproic acid","1,4-Benzodioxin-6-propanol","Glechomanolide*","3-hydroxy-1-methylpyrrolidin-2-one*","Trans-Zeatin-1-O-Glucoside","¦Ã-Glutamyl tyramine","L-Phenylalaninol","Deoxypodophyllotoxin","L-Methionine Sulfoxide","Horsfieldin*","2-Aminohexadecane-1,4-diol","Hexadecylsphingosine","2,4,2',4'-tetrahydroxy-3'-prenylchalcone","2-Aminotetradecane-1,5,11-triol","Trp-Arg","3-(3',5'-Dimethoxy-4'-hydroxy-benzylidene')-2-indolinone","Strictamine","N-Decanoyl nornicotine","Dihydrojasmone","Artemisinic acid","Lancerin; 4-C-Glucosyl-1,3,7-Trihydroxyxanthone","5,8-dimethoxy-3-methylnaphthalen-1-ol","Gly-Val-Val","Ethyl 3-coumarincarboxylate","8-methylnaphthalene-1,2-diol","Asp-Leu-Phe","Ala-Ser","1-Monomyristin","Adenosine*","Glucovanillin","Cyclo(VVFF)","LysoPC 17:0(2n isomer)*","LysoPE 14:0(2n isomer)*","Arg-Leu","1,2,5,7,8-pentahydroxy-3-methylanthracene-9,10-dione","Glycyl-tryptophan","4-methoxybenzamide","L-Alanyl-L-leucine","3,5-Di-tert-butylbenzoic acid","N-Acetylputrescine","5-Aminovaleric acid","2-Phenylethylamine","N-Methyl-4-aminobutyric acid","N-Feruloylputrescine","Glu-Phe-Ala","2'-Deoxyadenosine*","epiloliolide","9-Oxooctadeca-10,12-Dienoic Acid","acetophenone","L-Glutamine","Choline","2-Amino-7-methyltridecan-1-ol*","Homoproline","N-Monocinnamoylputrescine*","10,14,16-Octadecatrien-12-Ynoic Acid","N-(3-hydroxy-4-methoxyphenethyl)-4-hydroxybutanamide","3-Hydroxy-1-(4-Hydroxy-3-Methoxyphenyl)Propan-1-One","(6R,9R)-3-Oxo-¦Á-ionol-¦Â-D-glucoside*","DL-Methionine","N-Feruloylputrescine N-glucoside","Candicine","Sesamin","Ser-Phe-Met","Indoline","Isofraxetin","Aniline","Eleutheroside B1*","Val-Ala-Ile","Phe-Gly-Gly","N,N-Dimethylglycine*","Alnusonol","3,4-Dihydroxy-L-phenylalanine (L-Dopa)","3-(triazan-2-yl)propyl L-prolinate","Nicotine N-glucoside","Ent-16beta-Methoxy-19-Kauranoic Acid","6-Methylnicotine","2-Aminopurine","cis-Zeatin riboside","Hyp-Ser","isoacolamone","LysoPE 20:3*","2-(Dimethylamino)guanosine*","Glu-Ser","¦¡-hydroxyquinoline","Pro-Asn","Pro-Asp","L-¦Ã-Glutamyl-L-leucine","Eriodictyol-7-O-glucoronide","L-Aspartic acid","Cotinine-glucoside*","L-Homocysteine","LysoPC 18:1(2n isomer)","2-¦Á-Linolenoyl-glycerol*","LysoPC 16:1(2n isomer)*","1,8,15-triazacyclohenicosane-2,9,16-trione","6,7-dimethoxy-2-[2-(4'-hydroxy-3'-methoxyphenyl)ethyl]chromone","linolenoyl ethanolamine","Arg-Trp","Meso-dihydroguaiaretic acid","2-Methylthio-cis-zeatin riboside","Val-Ile-Gly","Camalexin","MG(18:3(9Z,12Z,15Z)/0:0/0:0)","Piperidine","Asn-Leu","2',4'-Dimethylacetophenone","Isoleucyl-Glutamyl-Leucine","Ser-Trp","9-Hydroxyselina-4,11-dien-14-al","p-Coumaric acid","Costunolide*","Asp-Ile-Leu","Monopalmitin*","Octadeca-2,9,12,15-tetraen-1-ol","2-Aminododecane-1,4-diol","Lithospermoside","Ehretioside B","Trp-Asn","N-Cis-Feruloyltyramine*","L-Isoleucyl-L-Aspartate","L-Alanyl-L-Phenylalanine","(3-(carboxyamino)-2-methylpropanoyl)phenylalanine","Lumazine","L-Aspartyl-L-Phenylalanine","N,N-cinnamoylbutanediamine*","Leu-Pro","Damnacanthol-11-methyl ether","2-Aminohexadecane-1,15-diol","L-Glutamine tert-butyl ester","Phellodendroside","Gly-Met-Met","10-nitro-9E-octadecenoic acid","1,7-bis(4-hydroxy-3-methoxyphenyl)hept-1-ene-3-ol","LysoPC 15:0(2n isomer)*","Nicotinate D-ribonucleoside","L-Dihomomethionine","Tetradecasphinganine","Calystegine B2","Val-Trp","alanine betaine","Scoparone","Biotin","Cyclo(Pro-Glu)","L-Homophenylalanine","5'-Deoxy-5'-(methylthio)adenosine","Hexaethyleneglycol","2-Phenylacetamide*","Guvacoline","Leu-Asp","Polygodial*","N-(4-Aminobutyl)benzamide","LysoPE 16:1*","3-ethyl-7-hydroxy-6-methoxyphthalide","N-Feruloylphenylacetamide","2-Ethynyl-5-methoxybenzo-1,4-quinone","Anabasine-glucoside","3'-Hydroxycotinine","Cinnamic acid","Epigoitrin","5-Hydroxy-2-pyrrolidinone","Nicotine-N-glucuronide*","4,5,6-Trihydroxy-2-cyclohexen-1-ylideneacetonitrile","4-Hydroxyderricin","Luteolin-7-O-glucoside (Cynaroside)*","Diferuloylcadaverine","Phellamurin","Cyclo(L-Phe-trans-4-hydroxy-L-Pro)","2'-O-Methyladenosine","Dicaffeoylshikimic acid","Lignans Machilin F","Stachydrine*","¦Ã-Glu-Trp","8-hydroxy-1,4a-dimethyl-7-(prop-1-en-2-yl)-4,4a,5,6,7,8-hexahydronaphthalen-2(3H)-one*","Cordycepin (3'-Deoxyadenosine)*","h-gamma-Glu-leu-oh","2-Feruloyl-sn-glycerol","Thr-Asn-Gly","N-Acetylcadaverine","Trans-4-Hydroxy-L-proline","methylalpha-aspartylphenylalaninate*","N-Acetyl-5-hydroxytryptamine","L-Norleucine*","Nicotinic Acid Methyl Ester(Methyl Nicotinate)","8-Azaguanine","Guanosine","N-Feruloyl-Cadaverine","Atractylenolide I","5-Methyl-2'-Deoxycytidine","2-Aminotetradecane-1,4-diol","N-Monomethyl-L-arginine*","1-methyl-1,3a,8,8a-tetrahydropyrrolo[2,3-b]indole","2-Aminohexadecane-1,16,16-triol","Tyr-Cys-Leu","H-Leu-Trp-OH","L-Pipecolic Acid","6-Hydroxynicotine","Tebuconazole","Phenylethanolamine","6-Methoxycoumarin","2-Aminoisobutyric acid","Scopoletin-7-O-xylosyl(1¡ú6)glucoside£¨Fabiatrin£©*","Tributyl acetylcitrate","Nicosesquiterpene E","LysoPC 17:0*","Nicosesquiterpene A","1,7-Bis-(4-hydroxyphenyl)-2,4,6-heptatrienone","Dihydroisoalantolactone","Neocnidilide*","2-Aminohexadecane-1,5,6-triol","Ala-Phe-Leu","LysoPC 16:2(2n isomer)*","Met-Ser","Isocurcumenol","Curdione","O-Phosphocholine","14-Amino-15-hydroxy-11-methylpentadecanoic acid","2-Aminooctadecane-1,5,17-triol","N-Hydroxyoctanoyl nornicotine glucoside","p-Coumaroyltyramine*","3-Hydrocinnamyl-6-CinnamylGlucose","Phe-Ser","10-Formyltetrahydrofolic Acid","Isocyperol","Glu-Val-Ile","Dehydrodiconiferyl alcohol","Val-Ile-Val","LysoPC 18:4","LysoPC 18:3","saikolignanoside D*","LysoPC 18:0","2-Methoxy-4-methylphenol","LysoPC 18:2","LysoPC 18:1","5,7-Dihydroxy-4-methylcoumarin","N-Methyl-¦Á-aminoisobutyric acid","Ser-Glu-Leu","1-Pyrrolizino-3-(3,4-Dihydroxyphenyl)-2-Propene-1-One","Tetradecyldiethanolamine","15(R)-Hydroxylinoleic Acid*","LysoPC 17:2*","N-Caffeoyltyramine; Typheramide*","D-Allo-Isoleucine*","3-(Pyrazol-1-yl)-L-alanine","Luteolin-4'-O-glucoside*","L-Tyrosine","Nornicotine","Dehydrocostuslactone","N-Trans-Sinapoyltyramine","Thr-Trp","2-¦Á-Linolenoyl-glycerol-1,3-di-O-glucoside","N-Feruloyloctopamine glucoside","N-(4-hydroxyphenethyl)cinnamamide","Thymol methyl ether","Glu-Pro-Leu","Neochlorogenic acid (5-O-Caffeoylquinic acid)","N,N'-Dimethylarginine;SDMA*","Cannabisin F","Triethylamine","Isoscopoletin (6-Hydroxy-7-Methoxycoumarin)","Isofraxidin","Ala-Ile-Asp","1,2,3,14,20,22,25-Heptahydroxycholest-7-en-6-one (Integristerone A)","Integerrimine","Rengyol","1-g-Linoleoyl-glycerol*","LysoPC 16:0","Val-Pro","2,5-dimethyl-2,3,3a,7a-tetrahydro-1H-indene-1,3-diol","1-Linoleoylglycerol*","Senkyunolide K","Senkyunolide M","Phe-Glu-Thr","N-Glucosylpicolinate","Ile-His","Senkyunolide A","Ala-Leu-Leu*","Senkyunolide B","L-Cyclopentylglycine","5,6,7,7a-tetrahydro-4,4,7a-trimethyl-2(4H)-benzofuranone","N6-(2-Hydroxyethyl)adenosine*","neopetasane*","3-(4-hydroxyphenyl)-n-[2-(4-hydroxyphenyl)ethyl]prop-2-enimidic acid","14-hydroxy-¦Á-humulene*","LysoPC 18:0(2n isomer)","Lyciumamide C","Ile-Val-Gly","4-Nitrophenol","Hydroxystyrene","Glu-Ile-Glu","Ala-Glu-Leu","Ile-Val-Glu","4-Methoxy-5-(hydroxymethyl)-2H-1-benzopyran-2-one","LysoPC 17:2(2n isomer)*","4-hydroxy-2-oxo-1,2-dihydroquinoline-3-carboxylic acid*","His-Glu","Balanophonin B","N,N-DimethyldecylamineN-oxide","3-(2-oxopropyl)-3-hydroxyindolin-2-one","Ser-Leu-Leu","N-Ethylglycine*","Methyl 3-aminopropanoate","Maesopsin","dihydro-N-feruloyltyramine","LysoPE 18:3","Monolinolenin*","2-Hexylphosphoric Acid","LysoPE 18:2","3-(((S)-1-carboxy-2-phenylethyl)amino)-2,5-dihydroxycyclopentane-1-carboxylic acid","5-Hydroxyflavone","3-Oxooleana-11,13(18)-dien-28-oic acid","3-Hydroxy-4-methoxybenzoic acid; Isovanillic Acid","6-Chloropurine","Betaine","Isochlorogenic acid C","Dihydroferuloylputrescine","abutasterone*","Curcumenol*","Butylphthalide","LysoPC 14:0","Ethyl isobutyrate","Daminozide","2(4H)-benzofuranone","¦Á-Cyperone","Epinootkatol","Trimethyllysine","Pyridine-4-formyl-O-¦Â-D-glucopyranoside","Cytidine","6,9-dihydroxymegastigmasta-5,7-dien-3-one9-O-beta-D-gentiobioside","Ser-Val-Leu","6-Methylflavone","Nicotine1-N-oxide","Isoquinoline","6-Hydroxy-N-methylmyosmine","9,11,13,15-Octadecatetraenal","L-Homocysteinesulfinic acid","2-Palmitoylglycerol*","Leu-Val-Leu","Pyran[3,4-b]indole-2-ketone","Pentaethyleneglycol","Acutissimalignan B*","Eugenol","N-Trans-feruloyl-3-O-methyldopamine*","4-amino-5-(butylamino)-5-oxopentanoic acid","Octylamine","2,5-Dimethoxybenzoquinone*","1-Arabinosyluracil","LysoPC 18:3(2n isomer)","Leu-Arg","LysoPE 14:0*","Ribosyladenosine","Pogostone","LysoPC 15:0*","3,3'-Oxydipropyl dibenzoate","5,8,11,14-Pentadecanoamide","Grossamide","Ranunculinin derivative","LysoPE 16:0","Asp-Ile-Glu","N-ethylcytisine","Isonicotinic acid","Ferulic acid methyl ester","Ile-Asn","N-propionyl-methyl-nornicotine","2-O-¦Á-D-glucosyl-1-deoxynojirimycin","N-Glycyl-L-leucine*","Matairesinol-4'-O-glucoside (Matairesinoside)*","Scopoletin-7-O-glucoside (Scopolin)","Elemol","4-Methylazetidine-2-Carboxylic acid*","4-Guanidinobutyric acid","Anabasine","2'-Deoxyinosine","Methyl 4-caffeoylquinate","L-Glycyl-L-phenylalanine*","LysoPC 18:2(2n isomer)","2-(4,5-Dihydroxy-2-(3-((4-hydroxyphenethyl)amino)-3-oxopropyl)phenyl)-3-(4-hydroxy-3,5-dimethoxyphenyl)-N-(4-hydroxyphenethyl)acrylamide","Oxoassoanine N-oxide","Tyr-Gly*","Gln-Val-Gln","2,2-dimethylchromene-6-carboxylic acid*","N-Acetyl-L-Methionine","Ile-Tyr","2'-Deoxycytidine","Dendronobilin I","N-Feruloyl-3-methoxytyramine*","Met-Met","Methoxyindoleacetic acid","Canusesnol F","JiangxiBaiyingsu I","3-O-caffeoyl quininyl arabinoside","N-(4-oxopentyl)-acetamide*","Cuscohygrine","Gly-Val-Leu","rosacorenol*","Asp-Leu-Ser","Lauryldiethanolamine","2-Aminooctadecane-1,16,18,18-tetraol","4-Hydroxycinnamic acid p-hydroxyphenethylamine","Lys-Phe","1-Monolinolenoyl-Rac-Glycerol*","Phellodensin F","5'-Deoxyadenosine*","Isofraxidin 7-O-Apiosylglucoside*","L-Phenylalanyl-L-phenylalanine","Serotonin","Gly-Leu-Val","4-amino-5-oxo-5-(pentylamino)pentanoic acid","2-[(5s)-5-hydroxyhexa-1,3-dien-1-yl]-5-methoxy-4-[(3r)-3-methoxybutanoyl]-2-methylfuran-3-one","1-Cis-5-trans-germacrone","tortoside f","Tyr-Gly-Thr","Cycloleucine","L-Homocitrulline","LysoPE 18:0","L-Praziquanamine","N-Benzylmethylene isomethylamine","Dehydrodiconiferyl alcohol-4-O-glucoside*","Arginine methyl ester*","N-Feruloylspermidine","L-Leucyl-L-phenylalanine","(S)-2-Phenyloxirane","Tetraethyleneglycol","Pro-Asn-Leu","11,12-O-Isopropyfidenesolajiangxin F","2-Methoxy-4-ethenylphenol","dihydrosesamin*","Glu-Ile-Val","Homoarginine","2-Linoleoylglycerol*","m-Aminophenylacetylene","Gly-Ile*","Roupelliol","Pyridoxine-5'-O-glucoside","Lauramine oxide","Hispanolone","Coumarin","Octadecadienamide","Leu-Thr-Ala","Glycidyl Linoleate","Gly-Phe-Glu","3-Indolepropionic acid","Lys-Ala","Spermine","2-Phenylethanol","Hypoxanthine","N6-methyladenosine","N-Hexanoyl nornicotine","N,N-Dimethyl-5-methoxytryptamine","1,4-Benzoquinone","9-Hydroxyselina-3,11-dien-12-al","L-Arginine","Glu-Phe*","Ser-Ile-Asp","N-Acetyl-DL-phenylalanine","1,3-O-Dicaffeoylquinic Acid (Cynarin)","Met-Ile-Glu","Cyclo(Ala-Gly)","Rishitin","Decursinol","Dehydrophytosphingosine","O-Feruloyl 4-hydroxycoumarin","N-methylphenylalanine","7,3',4'-Trihydroxyquercetin glucoside","Phe-Thr-Val","9,12-Octadecadien-6-Ynoic Acid","3,5-Dihydro-2H-Furo[3,2-C]Quinolin-4-One*","Cytarabine","Styrylamine","5,7-Dihydroxychromone glucoside","4-Hydroxy-3-methoxy-¦Â-phenethylamine","12,13-Dehydrogeranylgeraniol","2-Methoxy-1-benzofuran-5-carbaldehyde","¦Ã-glutamylmethionine","Adenine","Glu-Ile","10¦Á-9-oxoageraphorone","2-AminoicoSane-1,5,7,19-tetraol","¦Â-Nicotyrine","N6-Threonylcarbamoyladenosine","Lumichrome","Cimicifugamide","1-(3,4-Dihydroxyphenyl)-7-(4-hydroxyphenyl)hept-1-ene-3,5-dione","2-Pentyl-3-phenyl-2-propenal","Phylloquinone (Vitamin K1)","2-Aminooctadecane-1,4-diol","Ethyl phenylacetate","2-aminotetracoSane-1,6,7,23-tetraol*","(5-8)-Hydroxy-1-(hydroxylmethoxyphenyl)-N2,N3-bis(4-hydroxyphenethyl)-(5-8)-dimethoxy-1,2-dihydronaphthalene-2,3-dicarboxamide","Histidinol","Anhydro-¦Â-rotunol*","1-O-Feruloylquinic acid","1-epi-10¦Â-Hydroxycadin-4-en-15-al","Cis-5'-(2-oxopropyl)-nicotine*","Lys-Gly","Ile-Phe","1-(Hydroxymethyl)-5-pyridin-3-ylpyrrolidin-2-one","Ile-Ile-Asp","N1,N10-Bis(p-coumaroyl)spermidine","Caffeine","Thiamine (Vitamin B1)","Glu-Leu-Lys","Nootkatol","GrossamideK","Maltotriose","L-Methionine","4,5-Dihydrovmifoliol","Val-Pro-Leu","Gly-Phe*","Methyldopa","L-Valyl-L-Phenylalanine","2-g-linolenoyl-glycerol","Caffeoylmethylgluconamide","N-Trans-Feruloyl-3'-O-methyldopamine*","2-Amino-1,3-eicosanediol","LysoPC 16:0(2n isomer)","5-[(2r,3s,4s,5s)-5-(2h-1,3-benzodioxol-5-yl)-3,4-dimethyloxolan-2-yl]-2-methoxyphenol*","S-Methyl-L-cysteine","1-Hydroxybisabola-2,10-dien-4-one","Dimethyl phthalate","Ile-Trp","Mesitaldehyde","6-[(7-hydroxy-2-methyl-6-oxo-3,4-dihydroisoquinolin-1-yl)methyl]-2,3-dimethoxybenzoic acid","N-(p-hydroxybenzyl) adenosine","Sophoridine","Melongenaterpene H","N7-Methylguanosine","Pyroglutamic acid","3,4'-Dihydroxy-3',5'-dimethoxypropiophenone","tetrahydrochromone M","1-[(1,3-Benzodioxol-5-yl)carbonyl]-5-methoxyindoline","2,5-Dimethyl-7-hydroxychromone glucoside","N-Methylnicotinamide","Lys-Cys-Gln","LysoPE 20:4(2n isomer)*","Val-Leu-Asn","Hexadecyl ethanolamine","Thr-Leu-Glu","LysoPE 18:1*","1-Amino-1-cyclobutane-carboxylic-acid*","Dihydrocaffeoylputrescine","7-(4-Hydroxyphenyl)-1-phenyl-4-hepten-3-one","Cyclo(Phe-Glu)","Umckalin (7-hydroxy-5,6-dimethoxycoumarin)","LysoPE 15:0(2n isomer)*","2,5-O-Di-CinnamylSorbitol","3-amino-2-naphthoic acid*","9¦Â-hydroxyeudesma-4,11(13)-dien-12-al*","Thymidine","1-Methoxyphaseollin","2,6-Di-Tert-Butyl-1,4-Benzoquinone","3,5-Bis(3-methoxy-4-hydroxyphenyl)-2,3-dihydro-2(1H)-pyridinone*","Trans-3-Hydroxycotinine*","2-Ethyl-3-methylmaleimide-N-glucoside","4-MethoxycinnaMaldehyde","2-Picoline; 2-Methylpyridine","isoshyobunone","4-Methylbenzoic acid","N-(8-hydroxyoctyl)nornicotine","(6R,9R)-3-Oxo-¦Á-ionol-¦Â-D-malonyl-glucoside","cyclo-(Gly-Phe)","Alanylproline","N-(2-Hydroxy-4-methoxyphenyl)acetamide","cis-Jasmone","5-Hydroxy-DL-tryptophan(5-HTP)","2,3'-Bipyridine","Histamine","alpha-hexylcinnamaldehyde","9-Alpha-Ribofuranosyladenine*","2-Piperidone","1-carboxy-5-(pyridin-3-yl)-3,4-dihydro-2H-pyrrol-1-ium","Tyr-Val","Calycosin-7-O-glucoside","Asp-Lys*","2-Aminododecane-1,3,4-triol","N-Feruloyl dopamine*","¦Â-Ionone","9-Oxo-12Z-Octadecenoic acid*","3-Methylcytidine","Vanillin acetate","Santalic acid","DL-Leucine*","LysoPE 20:5","L-Leucine*","9,19-Cyclolanost-24-en-3-ol (Cycloartenol)","H-Gly-ala-phe-OH","LysoPE 20:2","6,7-dimethyl-4-(2,3,4,5-tetrahydroxypentyl)-1H-quinoxaline-2,3-dione","L-canavanine","Procurcumenol*","7-Methoxy-coumarin-8-yl-acetaldehyde","1-Cis-5-cis-germacrone","His-Val-Ile","cis-N-p-Coumaroyltyramine*","1-¦Á-Linolenoyl-glycerol-2,3-di-O-glucoside","Cnidilide*","2'-Deoxyguanosine","Ser-Val-Ser","2-aminotetracoSane-1,5,21,23-tetraol*","Isatin","ethyl-L-arginine","Phe-Ala","N-p-hydroxybenxoylputrescine"],"additional_accession":[]},"is_claimable":false,"name":"Bio-organic fertilizer alleviates tobacco continuous cropping obstacles by coordinately reprogramming plant metabolism and the rhizosphere microbiome","description":"Background Crop continuous cropping obstacles (CCO) severely threaten soil ecosystem health and sustainable agricultural production. Although bio-organic fertilizer (BF) has been widely applied to mitigate crop CCO, the underlying mechanisms remain poorly understood. Herein, an Aspergillus-amended BF was developed using an antagonistic strain isolated from tobacco rhizosphere. By integrating plant physiological responses with plant and rhizosphere metabolomics and microbiome analyses, we investigated the mechanisms underlying BF-mediated alleviation of tobacco CCO. Results We show that novel Aspergillus-amended BF markedly alleviates tobacco CCO by coordinately reprogramming host metabolism and the rhizosphere microbiome. BF application activated tobacco systemic acquired resistance through phenylalanine-derived salicylic acid signaling, as evidenced by the induction of PR genes, reactive oxygen species accumulation, and redox homeostasis regulation. Concurrently, BF significantly reshaped the composition, functional potential, and interaction networks of the rhizosphere microbiome, suppressing pathogen survival and virulence while enriching beneficial taxa, including Aspergillus, Flavobacterium, Pseudomonas, and Galbibacter, along with functional pathways related to quorum sensing and ABC transporters. BF also enhanced positive microbial interactions, thereby strengthening microbial network stability. Critically, microbiome reconstitution assays demonstrated that this protective effect depended on viable rhizosphere microbial communities, highlighting a causal role of the microbiome. Field trials further confirmed the efficacy of BF in mitigating tobacco CCO, resulting in a 31.0% reduction in disease incidence and significant improvements in economic traits compared with chemical fertilizer alone. Conclusions Collectively, our findings reveal a coordinated host-microbiome mechanism underlying BF-mediated alleviation of tobacco CCO and highlight microbiome priming as a promising strategy for microbiome-based disease suppression and sustainable agricultural production.","dates":{"publication":"2026-04-13","submission":"2026-04-13"},"accession":"MTBLS14267","cross_references":{"KEGG":["Cmmn012461","Cmbn003996","Cmsn002067","Cmjn004337","Cmyn001328","Cmsp002787","Cmsp003083","Cmyp009787"]}}