MetaboLights

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ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/m_MTBLS14268_LC-MS_negative_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/m_MTBLS14268_LC-MS_positive_reverse-phase_v2_maf.tsvftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/i_Investigation.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/a_MTBLS14268_LC-MS_negative_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/a_MTBLS14268_LC-MS_positive_reverse-phase.txtftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268/s_MTBLS14268.txtprimaryOK200ftp://ftp.ebi.ac.uk/pub/databases/metabolights/studies/public/MTBLS14268 The metabolic identification information was was obtained by searching the laboratory’s mzCloud, MZvault and Chemspider, at the same time, theoretical fragment are integrated and quantified.MetaboLightsPublicLiquid Chromatography MS - negative - reverse-phaseLiquid Chromatography MS - positive - reverse-phase The LC/MS system for metabolomics analysis is composed of Ultim3000 high performance liquid tandem Orbitrap Exploris 480 high resolution mass spectrometer. The column used is purchased from Waters Acquity UPLC HSS T3 column (1.8um 2.1*100mm) Positive ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile Negative ion mode: mobile phase A: 0.1% formic acid aqueous solution; mobile phase B: 0.1% formic acid acetonitrile Injection volume 1μLGinsenoside Rd2 improves allergic asthma by inhibiting PTGS2 enzyme activity.The Affiliated Hospital to Changchun University of Chinese MedicineWeijia LiSerummass spectrometry assay(1) Weigh 100 μL of the sample, add 500 μL of extraction solution containing internal standard (methanol:acetonitrile = 1:1, internal standard concentration 20 mg/L), and vortex for 30 seconds. (2) Sonicate for 10 min (in an ice-water bath). (3) Let stand at -20°C for 1 hour. (4) Centrifuge the sample at 12,000 rpm for 15 min at 4°C. (5) Carefully transfer 500 μL of the supernatant to an EP tube. (6) Dry the extract in a vacuum concentrator. (7) Reconstitute the dried metabolites with 160 μL of extraction solution (acetonitrile:water = 1:1). (8) Vortex for 30 seconds and sonicate in an ice-water bath for 10 minutes. (9) Centrifuge the sample at 12,000 rpm for 15 min at 4°C. (10) Carefully transfer 120 μL of the supernatant into a 2 mL injection vial. Take 10 μL from each sample and mix to prepare the QC sample for instrument analysis.Mus musculushttps://www.ebi.ac.uk/metabolights/MTBLS14268Weijia Li. Research Center of Traditional Chinese Medicine, The Affiliated Hospital to Changchun University of Chinese Medicine. 251459548@qq.com.The original data (.raw) file acquisited by LC-MS was imported by CD search Library software. Peak extraction, peak alignment, m/z and retention time correction were respectively performed by program. The peak alignment was performed on different samples based on a retention time deviation of 0.2 min and a mass deviation of 5 ppm for more accurate identification. The peak extraction was performed based on the set mass deviation of 5 ppm, signal intensity deviation of 30%, S/N ≥ 3, signal intensity ≥ 100000, and additive ions. The peak area was quantified, and the target ions were integrated.Treatment251459548@qq.comBlood samples were collected via the orbital sinus under appropriate anesthesia in accordance with institutional animal care guidelines. The samples were allowed to clot at room temperature for 30 minutes, followed by centrifugation at 4000 rpm for 10 minutes. The supernatant (serum) was collected for subsequent analysis.MetabolomicsMetabolomicsThermo Scientific LTQ OrbitrapMus musculusuntargeted analysisSerumginsenoside RdPTGS2Dionex UltiMate 3000arachidonic acid metabolic pathwayuntargeted metabolite profilingMetabolomicsThermo Scientific LTQ OrbitrapMus musculusuntargeted analysisSerumginsenoside RdPTGS2Dionex UltiMate 3000arachidonic acid metabolic pathwayuntargeted metabolite profiling The Q Orbitrap mass spectrometer was used for its ability to acquire MS/MS spectra on a data-dependent basis (DDA) during an LC/MS experiment. Scanning range selection m/z 67-1000. The parameters of the ESI ion source are as follows: Spray voltage: 3500V (positive ion mode) or -2500V (negative ion mode); Sheath gas flow rate:50 arb; Aux Gas flow rate:10 arb; Sweep gas flow rate:1arb; Ion Transfer Tube Temp:325°C; Vaporizer Temp 350°C.falseGinsenoside Rd2 improves allergic asthma by inhibiting PTGS2 enzyme activityThis study aims to elucidate the molecular mechanisms underlying the therapeutic effects of mountain-grown ginseng in allergic asthma and to systematically identify and characterize bioactive monomeric compounds responsible for these effects. We found that total saponins from mountain-grown ginseng significantly alleviated asthmatic phenotypes. Further multi-omics analyses revealed that these effects may be mediated through modulation of the arachidonic acid metabolic pathway, particularly by regulating the enzymatic activity of the rate-limiting enzyme PTGS2, thereby suppressing the production of PGE2. Subsequent component identification using high-performance liquid chromatography, combined with computational modeling, identified ginsenoside Rd2 as a key bioactive compound. In vitro cellular experiments, together with site-directed mutagenesis of the predicted binding sites, further confirmed that Rd2 exerts inhibitory effects on PTGS2 enzymatic activity through specific molecular interactions. Collectively, these findings provide mechanistic insights into the anti-asthmatic effects of mountain-grown ginseng and highlight Rd2 as a promising bioactive compound targeting PTGS2, offering potential therapeutic value for allergic asthma.2026-04-132026-04-13MTBLS14268