The online KEGG, HMDB database was used to annotate the metabolites by matching the exact molecular mass data (m/z) of samples with those from database. If a mass difference between observed and the database value was less than 10 ppm, the metabolite would be annotated and the molecular formula of metabolites would further be identified and validated by the isotopic distribution measurements. We also used a in-house fragment spectrum library of metabolites to validate the metabolite identidification.
"],"repository":["MetaboLights"],"study_status":["Public"],"ptm_modification":[""],"instrument_platform":["Liquid Chromatography MS - negative - reverse-phase","Liquid Chromatography MS - positive - reverse-phase"],"chromatography_protocol":["An ACQUITY UPLC HSS T3 column (100 mm×2.1 mm, 1.8 um, Waters) was used for separation. The mobile phase consists of phase A (5 mmol/L ammonium acetate + 5 mmol/L acetic acid + water) and phase B (acetonitrile). Gradient elution conditions were set as follows: 0~0.8 min, 2% B; 0.8~2.8 min , 2%~70% B; 2.8~5.6 min , 70%~90% B; 5.6~6.4 min , 90%~100% B; 6.4~8.0 min , 100% B;8.0~8.1 min , 100%~2% B; 8.1~10.0 min , 2% B. The flow rate is 0.35 mL/min. The injection volume for each sample was 4 μL. The column oven was maintained at 40°C.
"],"publication":["A bovine plasma-derived serum substitute enables continuous manufacturing of cultivated fish biomass."],"submitter_name":["Xuan Zhou"],"submitter_affiliation":["Ocean University of China"],"organism_part":["dorsal muscle tissue"],"technology_type":["mass spectrometry assay"],"disease":[""],"extraction_protocol":["Weigh 50 mg (±5 mg) of the sample and add 500 μL of 80 % icy methanol solution, sonicated and vortexed it. Incubated for 30 min at -20C to precipitate proteins, and centrifuged at 20000 g for 10 min at 4°C, supernatant centrifuged for 5 min again. The supernatant was transferred to a fresh vial for UPLC-HRMS analysis. The quality control (QC) sample was prepared by mixing an equal aliquot of the supernatant of samples.
"],"organism":["Larimichthys crocea"],"full_dataset_link":["https://www.ebi.ac.uk/metabolights/MTBLS14288"],"author":["Xuan Zhou. Qingdao Institute of Marine Bioresources for Nutrition and Health Innovation. 17852728285@163.com."],"data_transformation_protocol":["The acquired MS data pretreatments including peak picking, peak grouping, retention time correction, second peak grouping, and annotation of isotopes and adducts was performed using XCMS software. LC-MS raw data files were converted into mzXML format and then processed by the XCMS, CAMERA and metaX toolbox implemented with the R software. Each ion was identified by combining retention time (RT) and m/z data. Intensities of each peaks were recorded and a three dimensional matrix containing arbitrarily assigned peak indices (retention time-m/z pairs), sample names (observations) and ion intensity information (variables) was generated.
"],"study_factor":["Treatment"],"submitter_email":["17852728285@163.com"],"sample_collection_protocol":["Three-month-old juvenile large yellow croaker (Larimichthys crocea) were used as the source, and the dorsal muscle tissue from their back was dissected and collected to isolate muscle satellite cells for subsequent cell culture experiments.
"],"omics_type":["Metabolomics"],"study_design":["Metabolomics","Larimichthys crocea","untargeted analysis","Waters ACQUITY UPLC I-Class System","meat (chopped)","experimental blank","dorsal muscle tissue","AB SCIEX TripleTOF 6600","untargeted metabolite profiling"],"curator_keywords":["Metabolomics","Larimichthys crocea","untargeted analysis","Waters ACQUITY UPLC I-Class System","meat (chopped)","experimental blank","dorsal muscle tissue","AB SCIEX TripleTOF 6600","untargeted metabolite profiling"],"mass_spectrometry_protocol":["Please update this protocol description operated in both positive and negative electrospray ionization mode. ESI temperature is 500C. The voltage is +5000 volts in positive ion mode and -4500 volts in negative ion mode. The curtain gas pressure of the ion source is 30 psi, Gas 1 (Auxiliary gas) and Gas 2 (Sheath gas) pressures are both set to 60 psi. The mass spectrometric data were obtained with full scan and information dependent acquisition (IDA) modes. In one acquisition cycle, the full scan acquisition range is 60-1200 Da, and the full scan acquisition time is 150 ms. Then, the top 12 signal ions with a signal accumulation intensity of more than 100 were selected from the full scan for IDA scanning, the IDA acquisition range is 25-1200 Da, and the acquisition time is 30 ms. Dynamic exclusion is set to 4 s.
"],"additional_accession":[]},"is_claimable":false,"name":"A bovine plasma-derived serum substitute enables continuous manufacturing of cultivated fish biomass","description":"Cultivated fish meat offers a sustainable solution to marine stock depletion, yet industrial scalability is limited by the prohibitive cost of fetal bovine serum (FBS). We developed a cost-effective bovine plasma-derived serum substitute (bPCPE-LTUS) through low-temperature ultrasonication. This physical processing method attenuates complement and enzymatic liabilities while preserving essential growth-promoting cytokines and carrier proteins. Proteomic profiling identified a distinct secreted factor-enriched landscape in bPCPE-LTUS that effectively supports cell adhesion and proliferation. Long-term culture assays and transcriptomic analysis confirmed that 5% bPCPE-LTUS maintains stable expansion (>100 passages) and preserves the capacity for targeted myogenic and adipogenic differentiation in fish satellite cells. By integrating this supplement with edible gelatin microcarriers in a continuous 16 L stirred-tank bioreactor workflow, we achieved a cumulative output of 4.1 × 1010 cells and 1,962.18 g of wet biomass over a 47-day production campaign. The harvested biomass was formulated into structured fish cakes exhibiting nutritional profiles and textures comparable to conventional fillets. Crucially, bPCPE-LTUS enabled a 30-fold reduction in production costs, decreasing from over US$1,553.67/lb to US$50.48/lb. This study establishes a technically robust and economically viable pathway toward the low-cost production of cultivated seafood.","dates":{"publication":"2026-09-25","submission":"2026-04-15"},"accession":"MTBLS14288","cross_references":{}}